Mercurial > repos > aaronpetkau > assemblystats
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author | aaronpetkau |
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date | Sat, 04 Jul 2015 09:43:13 -0400 |
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<tool id="assemblystats" name="assemblystats" version="1.0.2"> <description>Summarise an assembly (e.g. N50 metrics)</description> <requirements> </requirements> <command interpreter="python"> assembly_stats_txt.py '$input_type' '$stats.extra_files_path' '$input_type' '$bucket' '$input' '$stats' '$sortedcontigs' '$histogrampng' '$summedcontigspng' '$histogramdata' '$summedcontigdata' </command> <inputs> <param help="Is this from an genomic (contig) or transcriptomic assembly (isotig) or are these raw reads (read)" label="Type of read" name="input_type" type="select"> <option selected="yes" value="contig">Contig (if from genomic assembly)</option> <option value="isotig">Isotig (if from transcriptomic assembly)</option> <option value="read">Raw reads from sequencer in FASTA format (useful for 454 data)</option> </param> <param falsevalue="" help="Use this to specify whether or not bin sizes of 1 should be used when plotting histograms" label="Output histogram with bin sizes=1" name="bucket" truevalue="-b" type="boolean" /> <param format="fasta" label="Source file in FASTA format" name="input" type="data" /> <param checked="false" help="If checked, all output files will be displayed. If not checked, only the file 'Assembly Statistics' will be provided." label="Return all output files" name="all_outputs" type="boolean" /> </inputs> <outputs> <data format="tabular" label="Assembly statistics - $input.display_name" name="stats" /> <data format="fasta" label="Sorted contigs - $input.display_name" name="sortedcontigs"> <filter>all_outputs is True</filter> </data> <data format="png" label="Histogram of contig sizes - $input.display_name" name="histogrampng"> <filter>all_outputs is True</filter> </data> <data format="png" label="Cumulative sum of contig sizes - $input.display_name" name="summedcontigspng"> <filter>all_outputs is True</filter> </data> <data format="tabular" label="Histogram data - $input.display_name" name="histogramdata"> <filter>all_outputs is True</filter> </data> <data format="tabular" label="Cumulative sum of contig size data - $input.display_name" name="summedcontigdata"> <filter>all_outputs is True</filter> </data> </outputs> <help> **Summarise assembly overview** This script is used to give summary statistics of an assembly or set of reads. Typically this is run after an assembly to evaluate gross features. # Gives back # - N50 # - num of contigs > 1 kb # - num of contigs # - Read or Contig Histogram and graphs. # - Summed contig length (by number of contigs, in sorted order) </help> </tool>