Mercurial > repos > aaronpetkau > flash
diff assembly_stats_txt.xml @ 0:44c401ebc424 draft
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author | aaronpetkau |
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date | Sat, 04 Jul 2015 08:57:35 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/assembly_stats_txt.xml Sat Jul 04 08:57:35 2015 -0400 @@ -0,0 +1,60 @@ +<tool id="assemblystats" name="assemblystats" version="1.0.2"> + <description>Summarise an assembly (e.g. N50 metrics)</description> + <requirements> + </requirements> + <command interpreter="python"> + assembly_stats_txt.py + '$input_type' '$stats.extra_files_path' + '$input_type' + '$bucket' + '$input' + '$stats' + '$sortedcontigs' + '$histogrampng' + '$summedcontigspng' + '$histogramdata' + '$summedcontigdata' + + </command> + <inputs> + <param help="Is this from an genomic (contig) or transcriptomic assembly (isotig) or are these raw reads (read)" label="Type of read" name="input_type" type="select"> + <option selected="yes" value="contig">Contig (if from genomic assembly)</option> + <option value="isotig">Isotig (if from transcriptomic assembly)</option> + <option value="read">Raw reads from sequencer in FASTA format (useful for 454 data)</option> + </param> + <param falsevalue="" help="Use this to specify whether or not bin sizes of 1 should be used when plotting histograms" label="Output histogram with bin sizes=1" name="bucket" truevalue="-b" type="boolean" /> + <param format="fasta" label="Source file in FASTA format" name="input" type="data" /> + <param checked="false" help="If checked, all output files will be displayed. If not checked, only the file 'Assembly Statistics' will be provided." label="Return all output files" name="all_outputs" type="boolean" /> + </inputs> + <outputs> + <data format="tabular" label="Assembly statistics - $input.display_name" name="stats" /> + <data format="fasta" label="Sorted contigs - $input.display_name" name="sortedcontigs"> + <filter>all_outputs is True</filter> + </data> + <data format="png" label="Histogram of contig sizes - $input.display_name" name="histogrampng"> + <filter>all_outputs is True</filter> + </data> + <data format="png" label="Cumulative sum of contig sizes - $input.display_name" name="summedcontigspng"> + <filter>all_outputs is True</filter> + </data> + <data format="tabular" label="Histogram data - $input.display_name" name="histogramdata"> + <filter>all_outputs is True</filter> + </data> + <data format="tabular" label="Cumulative sum of contig size data - $input.display_name" name="summedcontigdata"> + <filter>all_outputs is True</filter> + </data> + </outputs> + <help> +**Summarise assembly overview** + +This script is used to give summary statistics of an assembly or set of reads. Typically this is run after an assembly to evaluate gross features. + + +# Gives back +# - N50 +# - num of contigs > 1 kb +# - num of contigs +# - Read or Contig Histogram and graphs. +# - Summed contig length (by number of contigs, in sorted order) + </help> +</tool>