Mercurial > repos > alperkucukural > crisprseek
diff compare2sequences.xml @ 7:ca68df041d0d draft default tip
planemo upload
author | alperkucukural |
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date | Wed, 04 Nov 2015 16:46:58 -0500 |
parents | 10db17004ffa |
children |
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--- a/compare2sequences.xml Wed Nov 04 15:30:22 2015 -0500 +++ b/compare2sequences.xml Wed Nov 04 16:46:58 2015 -0500 @@ -12,45 +12,14 @@ ## code chunk: Load all required libraries quietly library(CRISPRseek, quietly=TRUE, warn.conflicts=FALSE,verbose = FALSE) -compare2Sequences("${s1.inputfile}", "${s2.inputfile}", inputNames=c("Seq1", "Seq2"), REpatternFile = system.file('extdata', 'NEBenzymes.fa', package = 'CRISPRseek'), max.mismatch="${maxmismatch}", searchDirection="${a.searchDirection}", findPairedgRNAOnly=${a.fg.fpg_only}, min.gap = "${a.fg.mingap}", max.gap = "${a.fg.maxgap}", gRNA.name.prefix = "gRNA", PAM.size = "${a.PAMsize}", gRNA.size = "${a.gRNAsize}", PAM = "${a.PAM}", PAM.pattern = "${a.PAMPattern}", outputDir, weights="${a.weights}" ) +compare2Sequences("${inputfile1}", "${inputfile2}", inputNames=c("Seq1", "Seq2"), REpatternFile = system.file('extdata', 'NEBenzymes.fa', package = 'CRISPRseek'), max.mismatch="${maxmismatch}", searchDirection="both", findPairedgRNAOnly=${a.fg.fpg_only}, min.gap = "${a.fg.mingap}", max.gap = "${a.fg.maxgap}", gRNA.name.prefix = "gRNA", PAM.size = "${a.PAMsize}", gRNA.size = "${a.gRNAsize}", PAM = "${a.PAM}", PAM.pattern = "${a.PAMPattern}", outputDir="./", weights="${a.weights}" ) ]]></configfile> </configfiles> <inputs> - <conditional name="s1"> - <param name="input_source" type="select" label="Select Input File Path" help="Sequence input file path that contains sequences to be searched for potential gRNAs"> - <option value="history">History</option> - <option value="FullPath">Free Text</option> - <option value="Fasta">Fasta</option> - </param> - <when value="FullPath"> - <param name="inputfile" type="text" area="true" size="2x60" label="Input File Path" help="Full path in the cluster"/> - </when> - <when value="Fasta"> - <param name="inputfile" type="text" area="true" size="2x60" label="Input Fasta Sequence/s" help="Free text fasta sequences"/> - </when> - <when value="history"> - <param format="fastq,fa,fasta" name="inputfile" type="data" label="Input File Path" /> - </when> - </conditional> - <conditional name="s2"> - <param name="input_source" type="select" label="Select Input File Path" help="Sequence input file path that contains sequences to be searched for potential gRNAs"> - <option value="history">History</option> - <option value="FullPath">Free Text</option> - <option value="Fasta">Fasta</option> - </param> - <when value="FullPath"> - <param name="inputfile" type="text" area="true" size="2x60" label="Input File Path" help="Full path in the cluster"/> - </when> - <when value="Fasta"> - <param name="inputfile" type="text" area="true" size="2x60" label="Input Fasta Sequence/s" help="Free text fasta sequences"/> - </when> - <when value="history"> - <param format="fastq,fa,fasta" name="inputfile" type="data" label="Input File Path" /> - </when> - </conditional> + <param format="fastq,fa,fasta" name="inputfile1" type="data" label="Input File Path" /> + <param format="fastq,fa,fasta" name="inputfile2" type="data" label="Input File Path" /> <param name="maxmismatch" type="text" label="Max Mismatch" value="3" help="Maximum mismatch allowed in off target search, default 3. Warning: will be considerably slower if set > 3"/> - <param name="outputdir" type="text" area="true" size="2x60" label="Output Directory" help="Output directory in the cluster"/> <conditional name="a"> <param name="advanced" type="select" label="Advanced Options"> <option value="No" selected="True">No</option>