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1 <tool id='align_and_estimate_abundance' name='Align and Estimate Abundance' version='r20140717'>
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2
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3 <description> using utility align_and_estimate_abundance.pl</description>
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4
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5 <requirements>
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6 <requirement type="package" version="r20140717">trinityrnaseq</requirement>
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7 <requirement type="package" version="0.12.7">bowtie</requirement>
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3
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8 <requirement type="package" version="2.2.5">bowtie2</requirement>
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2
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9 <requirement type="package" version="1.2">samtools</requirement>
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0
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10 <requirement type="package" version="1.5.1">eXpress</requirement>
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11 </requirements>
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12
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13 <command> \$TRINITY_HOME/util/align_and_estimate_abundance.pl
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14 --transcripts $transcripts
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15 --est_method $est_method
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16 --aln_method $aln_method
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17 --prep_reference
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18
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19 ## Inputs.
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20 #if str($reads.paired_or_single) == "paired":
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21
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22 --left $reads.left_input --right $reads.right_input
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23
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24 #if $reads.left_input.ext == 'fa':
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25 --seqType fa
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26 #else:
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27 --seqType fq
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28 #end if
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29
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30 ## Additional parameters.
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31 #if str($reads.optional.use_options) == "yes":
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32
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33 #if str($reads.optional.library_type) != "None":
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34 --SS_lib_type $reads.optional.library_type
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35 #end if
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36
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37
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38
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39 #end if
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40
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41 #else:
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42 --single $reads.input
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43
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44 #if str($reads.input.ext) == 'fa':
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45 --seqType fa
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46 #else:
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47 --seqType fq
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48 #end if
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49
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50 ## Additional parameters.
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51 #if str($reads.optional.use_option) == "yes":
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52
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53 #if str($reads.additional_params.library_type) != "None":
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54 --SS_lib_type $reads.optional.library_type
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55 #end if
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56
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57 #end if
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58 #end if
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59
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60 ## direct to output
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61 > $align_and_estimate_abundance_log 2>&1
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62
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63 </command>
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64
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1
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65 <inputs>
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66
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67 <!-- Required -->
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68 <param name="transcripts" type="data" format="fasta" label="Transcripts [ in fasta format ]" help="--transcripts < string > ; transcript fasta file"/>
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69
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70 <param name="est_method" type="select" display="radio" label="Choose abundance estimation method" help="">
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71 <option value="RSEM" selected="True">RSEM</option>
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72 <option value="eXpress">eXpress</option>
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73 </param>
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74
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75 <param name="aln_method" type="select" display="radio" label="Choose alignment method" help="">
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76 <option value="bowtie" selected="True">bowtie</option>
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77 <option value="bowtie2">bowtie2</option>
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78 </param>
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79
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80 <conditional name="reads">
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81 <param name="paired_or_single" type="select" label="Paired or Single-end reads?">
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82 <option value="paired">Paired</option>
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83 <option value="single">Single</option>
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84 </param>
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85
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86
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87 <when value="paired">
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88 <param format="fasta,fastq" name="left_input" type="data" label="Left/Forward strand reads" help=""/>
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89 <param format="fasta,fastq" name="right_input" type="data" label="Right/Reverse strand reads" help=""/>
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90
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91 <param name="seqtype" type="select" label="--seqType" help="">
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92 <option value='fq' selected='true'>fastq</option>
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93 <option value='fa'>fasta</option>
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94 </param>
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95
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96 <conditional name='optional'>
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97 <param name='use_options' type='select' label='Use Optional parameters?'>
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98 <option value='no'>NO</option>
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99 <option value='yes'>YES</option>
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100 </param>
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101 <when value='no'/>
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102 <when value='yes'>
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103 <param name="library_type" type="select" label="Strand-specific Library Type">
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104 <option value="None">None</option>
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105 <option value="FR">FR</option>
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106 <option value="RF">RF</option>
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107 </param>
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108
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109
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110
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111 </when>
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112 </conditional>
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113 </when>
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114 <when value="single">
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115 <param format="fasta,fastq" name="input" type="data" label="Single-end reads" help=""/>
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116 <param name="seqtype" type="select" label="--seqType" help="">
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117 <option value='fq' selected='true'>fastq</option>
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118 <option value='fa'>fasta</option>
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119 </param>
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120
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121 <conditional name='optional'>
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122 <param name='use_options' type='select' label='Use Optional parameters?'>
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123 <option value='no'>NO</option>
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124 <option value='yes'>YES</option>
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125 </param>
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126 <when value='no'/>
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127 <when value='yes'>
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128 <param name="library_type" type="select" label="Strand-specific Library Type">
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129 <option value="None">None</option>
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130 <option value="F">F</option>
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131 <option value="R">R</option>
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132 </param>
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133 </when>
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134 </conditional>
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135 </when>
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136 </conditional>
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137
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138 </inputs>
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139
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140 <outputs>
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141 <data format="txt" name="align_and_estimate_abundance_log" label="${tool.name} on ${on_string}: log" />
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142 <data format="bam" name="bowtie_bam" label="${tool.name} on ${on_string}: bam" from_work_dir="bowtie.bam" />
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143 <data format="tabular" name="rsem_isoforms" label="${tool.name} on ${on_string}: isoforms" from_work_dir="RSEM.isoforms.results"/>
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144 <data format="tabular" name="rsem_genes" label="${tool.name} on ${on_string}: genes" from_work_dir="RSEM.genes.results"/>
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145 </outputs>
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146 </tool>
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147
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