# HG changeset patch # User antmarge # Date 1490831725 14400 # Node ID 2efae0932a7a68f03e33a59798e4c0a09d6aed84 # Parent d37b0646ed292b2872d8f6566aa77b1e7838de09 Uploaded diff -r d37b0646ed29 -r 2efae0932a7a compGenes.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/compGenes.xml Wed Mar 29 19:55:25 2017 -0400 @@ -0,0 +1,51 @@ + + + + + compare gene aggregate fitness from two different experiments + + + perl + perl_getopt_long + perl_statistics_distributions + + + + compGenes.pl + -input1 $input1 + -input2 $input2 + -l1 $l1 + -l2 $l2 + -o $outfile + + + + + + + + + + + + + + + **What it does** + + This tool compares genes for an organism under different conditions. It takes two geneAgregate outputs and compares them calculating the difference in mean fitness for each gene. Example usage: compare organism in presence of control vs antibiotic. For different strains/genomes, where gene ids are not the same, use compStrains.pl; + + + **The options explained** + + Input 1 and 2: These are the csv (comma separated values) files containing the gene fitness values. Since they should have been produced by the "Aggregate Fitness" tool, each line besides the header should represent the following information for a gene: Locus, Fitness, NumberofInsertions, StandardDev of insertion fitness, Standard Error of insertion fitness, and Marking + + Label 1 and 2: Labels for the column headers for files in the comparison + + + The name of your output file: self-explanatory. Remember to have it end in ".csv". + + + + +