Mercurial > repos > anton > vcfgeno2haplo
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author | anton |
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date | Mon, 15 Sep 2014 14:51:43 -0400 |
parents | a6cd013745fd |
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<tool id="vcfgeno2haplo" name="VCFgenotype-to-haplotype:" version="0.0.2"> <requirements> <requirement type="package" version="8a5602bf07">vcflib</requirement> </requirements> <description>Convert genotype-based phased alleles into haplotype alleles</description> <command> #set $reference_fasta_filename = "localref.fa" #if str( $reference_source.reference_source_selector ) == "history": ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && #else: #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) #end if vcfgeno2haplo ${output_option} -w ${window_size} -r "${reference_fasta_filename}" "${input_vcf}" > "${out_file1}"</command> <inputs> <conditional name="reference_source"> <param name="reference_source_selector" type="select" label="Choose the source for the reference genome"> <option value="cached">Locally cached</option> <option value="history">History</option> </param> <when value="cached"> <param name="ref_file" type="select" label="Select reference genome"> <options from_data_table="fasta_indexes"> <!--<filter type="data_meta" key="dbkey" ref="input_bam" column="value"/>--> </options> <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> </param> <param name="input_vcf" type="data" format="vcf" label="Select VCF dataset"> <!-- Validators are commented to allow users apply too to any build. May need to be revised in the future <validator type="unspecified_build" /> <validator type="dataset_metadata_in_data_table" table_name="fasta_indexes" metadata_name="dbkey" metadata_column="1" message="Sequences are not currently available for the specified build." /> --> </param> </when> <when value="history"> <!-- FIX ME!!!! --> <param name="ref_file" type="data" format="fasta" label="Using reference file" /> <param name="input_vcf" type="data" format="vcf" label="Select VCF dataset" /> </when> </conditional> <param name="window_size" type="text" size="4" value="30" label="compare records up to this many bp away" help="--window-size option (default = 30)" /> <param name="output_option" type="boolean" truevalue="-o" falsevalue="" label="What to report?" help="--only-variants option" /> <!-- <option value=" ">Output entire haplotype</option> <option value="-o">Don't output the entire haplotype, just concatenate REF/ALT strings (delimited by ":")</option> </param> --> </inputs> <outputs> <data format="vcf" name="out_file1" /> </outputs> <stdio> <exit_code range="1:" level="fatal" /> </stdio> <tests> <test> <param name="reference_source_selector" value="history" /> <param name="output_option" value="true" /> <param name="window_size" value="5000" /> <param name="input_vcf" value="vcflib-phix.vcf"/> <param name="ref_file" value="vcflib-test-genome-phix.fa" /> <output name="out_file1" file="vcfgeno2haplo-test1.vcf"/> </test> </tests> <help> Convert genotype-based phased alleles within a window size specified by -w option into haplotype alleles. Will break haplotype construction when encountering non-phased genotypes on input. The options are:: -w, --window-size N Merge variants at most this many bp apart (default 30) -o, --only-variants Don't output the entire haplotype, just concatenate REF/ALT strings (delimited by ":") ---- Vcfgeno2haplo is a part of VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib). </help> </tool>