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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/bamparse commit 4e42cba873625fad03423e65dfffbf4afa91598c
author artbio
date Fri, 13 Oct 2017 02:59:36 -0400
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1 <tool id="bamparse" name="Count alignments" version="2.0.0">
2 <description>in a BAM file</description>
3 <requirements>
4 <requirement type="package" version="1.1.2">bowtie</requirement>
5 <requirement type="package" version="1.11.2">numpy</requirement>
6 <requirement type="package" version="0.11.2.1">pysam</requirement>
7 </requirements>
8 <stdio>
9 <exit_code range="1:" level="fatal" description="Tool exception" />
10 </stdio>
11 <command detect_errors="exit_code"><![CDATA[
12 mkdir outputdir &&
13 #for $file in $input_list
14 samtools index '$file' &&
15 #end for
16 python $__tool_directory__/bamparse.py
17 --alignments
18 #for $file in $input_list
19 '$file'
20 #end for
21 --labels
22 #for $file in $input_list
23 '$file.element_identifier'
24 #end for
25 --polarity '$polarity'
26 --number '$output_option'
27 ]]></command>
28 <inputs>
29 <param name="input_list" type="data" format="bam" label="Select multiple alignments to parse" multiple="true"/>
30 <param name="polarity" type="select" label="how to count sense and antisense reads">
31 <option value="both">count both sense and antisense reads</option>
32 <option value="sense">count only sense reads</option>
33 <option value="antisense">count only antisense reads</option>
34 </param>
35 <param name="output_option" type="select" display="radio" label="Select the number of files for results"
36 help="Results can be returned either as a single multi-column table or in separate two-column
37 datasets (useful for DESeq subsequent analysis)">
38 <option value="unique">A multi-column table</option>
39 <option value="multiple">One separate two-columns dataset per input bam alignment</option>
40 </param>
41 </inputs>
42 <outputs>
43 <data name="output" format="tabular" label="Counts">
44 <discover_datasets pattern="(?P&lt;designation&gt;.*)\.tabular" ext="tabular" visible="true" assign_primary_output="true" directory="outputdir"/>
45 </data>
46 </outputs>
47 <help>
48
49 **What it does**
50
51 Counts the number of reads aligned to each reference (@SN, reference NAME) in one or
52 several BAM alignments.
53
54 Sense, antisense or both sense and antisense alignments can be counted
55
56 The library labels in the returned count table are taken from the input bam datasets
57 names in the Galaxy history.
58 </help>
59 <tests>
60 <test>
61 <param name="input_list" value="alignment1.bam,alignment2.bam" ftype="tabular" />
62 <param name="polarity" value="both" />
63 <param name="output_option" value="unique" />
64 <output name="output" ftype="tabular" file="table.tabular" />
65 </test>
66 <test>
67 <param name="input_list" value="alignment1.bam,alignment2.bam" ftype="tabular" />
68 <param name="polarity" value="both" />
69 <param name="output_option" value="multiple" />
70 <output name="output" ftype="tabular" file="table0.tabular">
71 <discovered_dataset designation="table1" ftype="tabular" file="table1.tabular" />
72 </output>
73 </test>
74 </tests>
75 </tool>