# HG changeset patch # User artbio # Date 1562866280 14400 # Node ID bcbd7179d8ecd9c9e2c0cea115da8ff1da4eb963 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_center_scale commit b839b440f0760ff9cd75969d418432702947a669 diff -r 000000000000 -r bcbd7179d8ec center_scale.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/center_scale.R Thu Jul 11 13:31:20 2019 -0400 @@ -0,0 +1,101 @@ +options( show.error.messages=F, + error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) +loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") +warnings() +library(optparse) + +# Arguments +option_list = list( + make_option( + '--data', + default = NA, + type = 'character', + help = "Input file that contains values to transform. Must be tabular separated, + with columns and row names, variables in rows, observations in columns [default : '%default' ]" + ), + make_option( + '--center', + default = TRUE, + type = 'logical', + help = "center data to the mean [default : '%default' ]" + ), + make_option( + '--scale', + default = TRUE, + type = 'logical', + help = "scale data to standard deviation [default : '%default' ]" + ), + make_option( + '--factor', + default = '', + type = 'character', + help = "A two-column observations|factor_levels table, to group observations to be transformed by levels [default : '%default' ]" + ), + make_option( + '--output', + default = 'res.tab', + type = 'character', + help = "Table of transformed values [default : '%default' ]" + ) +) + +transform <- function(df, center=TRUE, scale=TRUE) { + transfo <- scale( + t(df), + center=center, + scale=center + ) + return(as.data.frame(t(transfo))) +} + +opt = parse_args(OptionParser(option_list = option_list), + args = commandArgs(trailingOnly = TRUE)) + +data = read.table( + opt$data, + check.names = FALSE, + header = TRUE, + row.names = 1, + sep = '\t' +) + +if (opt$factor != '') { + data.factor = read.table( + opt$factor, + check.names = FALSE, + header = TRUE, + sep = '\t' + ) + colnames(data.factor) <- c("cellid", "level") + data.transformed <- data.frame(row.names=rownames(data), stringsAsFactors=FALSE) + for (group in levels(data.factor$level)){ + subcells <- as.data.frame(subset(data.factor, level==group, select=cellid)) + subdata <- as.data.frame(subset(data, select=subcells$cellid)) + subdata.transformed <- transform(subdata, center=opt$center, scale=opt$scale) + data.transformed <- cbind(data.transformed, subdata.transformed) + } +} else { + data.transformed <- transform(data, center=opt$center, scale=opt$scale) +} + + +write.table( + cbind(gene=rownames(data.transformed), data.transformed), + opt$output, + col.names = TRUE, + row.names = FALSE, + quote = F, + sep = "\t" +) + + + + + + + + + + + + diff -r 000000000000 -r bcbd7179d8ec center_scale.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/center_scale.xml Thu Jul 11 13:31:20 2019 -0400 @@ -0,0 +1,105 @@ + + + + r-optparse + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +**What it does** + +The tool perform various normalization operations on a data table, including mean centering, +standard deviation rescaling, or both (standardization). + +In addition, these operations can be performed on subsets of observations, is the user provides +a two-column table that maps observations to groups (factor levels). + +**Inputs** + +A data table with observations (samples, library sequencings) in columns (library names +are column names) and variables (genes) in rows (gene names are row names). + +Optionally, a two-column table may be used to map observations to a factor level. In this +case, normalization operations will be performed by group having same level. + +**Outputs** + +A table with same numbers of columns and rows as input table, and transformed values. + + + + + @Manual{, + title = {R: A Language and Environment for Statistical Computing}, + author = {{R Core Team}}, + organization = {R Foundation for Statistical Computing}, + address = {Vienna, Austria}, + year = {2014}, + url = {http://www.R-project.org/}, + } + + + diff -r 000000000000 -r bcbd7179d8ec test-data/full_center.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/full_center.tab Thu Jul 11 13:31:20 2019 -0400 @@ -0,0 +1,24 @@ +gene V1 V2 V3 V4 V5 V6 V7 V8 V9 V10 V11 V12 V13 V14 V15 V16 V17 V18 V19 V20 V21 V22 V23 V24 V25 V26 V27 V28 V29 V30 V31 V32 V33 V34 V35 V36 V37 V38 V39 V40 V41 V42 V43 V44 V45 V46 V47 V48 V49 V50 V51 V52 V53 V54 V55 V56 V57 V58 V59 V60 V61 V62 V63 V64 V65 V66 V67 V68 V69 V70 V71 V72 V73 V74 V75 V76 V77 V78 V79 V80 V81 V82 V83 V84 V85 V86 V87 V88 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