Mercurial > repos > artbio > gsc_high_dimensions_visualisation
comparison high_dim_visu.xml @ 4:8e17c31c536a draft
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_high_dimension_visualization commit 1282ac9de7c926ab251f88afb2453f52c8b14200
author | artbio |
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date | Thu, 11 Jul 2019 12:31:28 -0400 |
parents | 8e44c9e18a56 |
children | 569334568afa |
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1 <tool id="high_dimensions_visualisation" name="Generate PCA, tSNE and HCPC" version="0.9.3"> | 1 <tool id="high_dimensions_visualisation" name="Generate PCA, tSNE and HCPC" version="0.9.4"> |
2 <description>from highly dimensional expression data</description> | 2 <description>from highly dimensional expression data</description> |
3 <requirements> | 3 <requirements> |
4 <requirement type="package" version="1.3.2=r3.3.2_0">r-optparse</requirement> | 4 <requirement type="package" version="1.6.2=r35h6115d3f_0">r-optparse</requirement> |
5 <requirement type="package" version="1.39=r3.3.2_0">r-factominer</requirement> | 5 <requirement type="package" version="1.42=r35h6115d3f_0">r-factominer</requirement> |
6 <requirement type="package" version="1.0.5=r3.3.2_0">r-factoextra</requirement> | 6 <requirement type="package" version="1.0.5">r-factoextra</requirement> |
7 <requirement type="package" version="0.13=r3.3.2_0">r-rtsne</requirement> | 7 <requirement type="package" version="0.15=r351he1b5a44_0">r-rtsne</requirement> |
8 <requirement type="package" version="2.2.1=r3.3.2_0">r-ggplot2</requirement> | 8 <requirement type="package" version="0.4.7=r351h6115d3f_0">r-ggfortify</requirement> |
9 <requirement type="package" version="0.4.1=r3.3.2_0">r-ggfortify</requirement> | 9 <requirement type="package" version="1.1.9=r351h0357c0b_0">r-clusterr</requirement> |
10 </requirements> | 10 </requirements> |
11 <stdio> | 11 <stdio> |
12 <exit_code range="1:" level="fatal" description="Tool exception" /> | 12 <exit_code range="1:" level="fatal" description="Tool exception" /> |
13 </stdio> | 13 </stdio> |
14 <command detect_errors="exit_code"><![CDATA[ | 14 <command detect_errors="exit_code"><![CDATA[ |
51 --HCPC_itermax '$visualisation.HCPC_itermax' | 51 --HCPC_itermax '$visualisation.HCPC_itermax' |
52 --HCPC_min '$visualisation.HCPC_min' | 52 --HCPC_min '$visualisation.HCPC_min' |
53 --HCPC_max '$visualisation.HCPC_max' | 53 --HCPC_max '$visualisation.HCPC_max' |
54 --HCPC_clusterCA '$visualisation.HCPC_clusterCA' | 54 --HCPC_clusterCA '$visualisation.HCPC_clusterCA' |
55 --HCPC_kk '$visualisation.HCPC_kk' | 55 --HCPC_kk '$visualisation.HCPC_kk' |
56 #if $visualisation.res_clustering == "yes": | |
57 --HCPC_clust '$HCPC_clust' | |
58 #end if | |
56 #end if | 59 #end if |
57 | 60 |
58 #if $visualisation.visu_choice == "PCA": | 61 #if $visualisation.visu_choice == "PCA": |
59 --PCA_npc '$visualisation.PCA_npc' | 62 --PCA_npc '$visualisation.PCA_npc' |
63 --PCA_x_axis '$visualisation.PCA_x_axis' | |
64 --PCA_y_axis '$visualisation.PCA_y_axis' | |
60 #end if | 65 #end if |
61 | 66 |
62 | 67 #if $visualisation.visu_choice == "HCPC" and $factor_condition.factor_choice == "Yes": |
68 --mutual_info '$mutual_info' | |
69 #end if | |
70 | |
63 --pdf_out '$pdf_out' | 71 --pdf_out '$pdf_out' |
64 | 72 |
65 ]]></command> | 73 ]]></command> |
66 <inputs> | 74 <inputs> |
67 <param name="input" type="data" format="txt,tabular" label="expression data"/> | 75 <param name="input" type="data" format="txt,tabular" label="expression data"/> |
109 </param> | 117 </param> |
110 <param name="Rtsne_pca_scale" type="select" label="Scalling data" help="Should data be scaled before pca is applied? " > | 118 <param name="Rtsne_pca_scale" type="select" label="Scalling data" help="Should data be scaled before pca is applied? " > |
111 <option value="TRUE">Yes</option> | 119 <option value="TRUE">Yes</option> |
112 <option value="FALSE" selected="true">False</option> | 120 <option value="FALSE" selected="true">False</option> |
113 </param> | 121 </param> |
114 <param name="Rtsne_normalize" type="select" label="Normalisation of data" | 122 <param name="Rtsne_normalize" type="select" label="Normalisation of data" |
115 help="Should variables (gene expressions) be normalized internally prior to distance calculations? " > | 123 help="Should variables (gene expressions) be normalized internally prior to distance calculations? " > |
116 <option value="TRUE" selected="true">Yes</option> | 124 <option value="TRUE" selected="true">Yes</option> |
117 <option value="FALSE">False</option> | 125 <option value="FALSE">False</option> |
118 </param> | 126 </param> |
119 <param name="Rtsne_perplexity" value="10.0" type="float" label="perplexity (t-SNE)" help="should be less than ((nbr observations)-1)/3" /> | 127 <param name="Rtsne_perplexity" value="10.0" type="float" label="perplexity (t-SNE)" help="should be less than ((nbr observations)-1)/3" /> |
120 <param name="Rtsne_theta" value="1.0" type="float" label="theta (t-SNE)"/> | 128 <param name="Rtsne_theta" value="1.0" type="float" label="theta (t-SNE)"/> |
121 <param name="Rtsne_exaggeration_factor" value="12.0" type="float" label="Exageration factor" help="Exaggeration factor used to multiply the P matrix in the first part of the optimization" /> | 129 <param name="Rtsne_exaggeration_factor" value="12.0" type="float" label="Exageration factor" help="Exaggeration factor used to multiply the P matrix in the first part of the optimization" /> |
122 <param name="Rtsne_max_iter" value="1000" type="integer" label="Number of iterations (default: 1000)" | 130 <param name="Rtsne_max_iter" value="1000" type="integer" label="Number of iterations (default: 1000)" |
152 <option value="rows" selected="true">Rows</option> | 160 <option value="rows" selected="true">Rows</option> |
153 <option value="cols">Columns</option> | 161 <option value="cols">Columns</option> |
154 </param> | 162 </param> |
155 <param name="HCPC_kk" value="-1" type="text" label="kk, Number of clusters used in a Kmeans preprocessing " | 163 <param name="HCPC_kk" value="-1" type="text" label="kk, Number of clusters used in a Kmeans preprocessing " |
156 help="No k-means consolidation is done if a kk value is provided (default=-1)" /> | 164 help="No k-means consolidation is done if a kk value is provided (default=-1)" /> |
165 <param label="Return HCPC clustering table" name="res_clustering" type="select"> | |
166 <option value="no" selected="True">No</option> | |
167 <option value="yes">Yes</option> | |
168 </param> | |
157 </when> | 169 </when> |
158 <when value="PCA"> | 170 <when value="PCA"> |
159 <param name="PCA_npc" value="5" type="integer" label="Number of principal components to keep" help="The number of dimensions which are kept for PCA analysis (default=5)" /> | 171 <param name="PCA_npc" value="5" type="integer" label="Number of principal components to keep" help="The number of dimensions which are kept for PCA analysis (default=5)" /> |
172 <param name="PCA_x_axis" value="1" type="integer" label="First principal component to plot" help="X axis for PCA plot (default=1)" /> | |
173 <param name="PCA_y_axis" value="2" type="integer" label="Second principal component to plot" help="Y axis for PCA plot (default=2)" /> | |
160 </when> | 174 </when> |
161 </conditional> | 175 </conditional> |
162 <param label="Return scatter plot table coordinates" name="coord" type="select"> | 176 <param label="Return scatter plot table coordinates" name="coord" type="select"> |
163 <option value="no" selected="True">No</option> | 177 <option value="no" selected="True">No</option> |
164 <option value="yes">Yes</option> | 178 <option value="yes">Yes</option> |
168 <outputs> | 182 <outputs> |
169 <data name="pdf_out" format="pdf" label="${visualisation.visu_choice} of ${on_string}" /> | 183 <data name="pdf_out" format="pdf" label="${visualisation.visu_choice} of ${on_string}" /> |
170 <data name="table_coordinates" format="tabular" label="Scatter plot coordinates from ${visualisation.visu_choice} of ${on_string}" > | 184 <data name="table_coordinates" format="tabular" label="Scatter plot coordinates from ${visualisation.visu_choice} of ${on_string}" > |
171 <filter>coord == 'yes'</filter> | 185 <filter>coord == 'yes'</filter> |
172 </data> | 186 </data> |
187 <data name="mutual_info" format="txt" label="External validation of clustering from ${visualisation.visu_choice} of ${on_string}" > | |
188 <filter>visualisation['visu_choice'] == 'HCPC' and factor_condition['factor_choice'] == 'Yes'</filter> | |
189 </data> | |
190 <data name="HCPC_clust" format="tabular" label="Clustering table from ${visualisation.visu_choice} of ${on_string}" > | |
191 <filter>visualisation['visu_choice'] == 'HCPC' and visualisation['res_clustering'] == 'yes'</filter> | |
192 </data> | |
173 </outputs> | 193 </outputs> |
174 <tests> | 194 <tests> |
175 <!-- test PCA --> | 195 <!-- test PCA --> |
176 <test> | 196 <test> |
177 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 197 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
185 <param name="labels" value="no" /> | 205 <param name="labels" value="no" /> |
186 <param name="visu_choice" value="PCA" /> | 206 <param name="visu_choice" value="PCA" /> |
187 <param name="factor_choice" value="No" /> | 207 <param name="factor_choice" value="No" /> |
188 <output name="pdf_out" file="pca.nolabels.pdf" ftype="pdf"/> | 208 <output name="pdf_out" file="pca.nolabels.pdf" ftype="pdf"/> |
189 </test> | 209 </test> |
210 <!-- test PCA PC2 vs PC3 --> | |
211 <test> | |
212 <param name="input" value="cpm_input.tsv" ftype="txt"/> | |
213 <param name="labels" value="no" /> | |
214 <param name="visu_choice" value="PCA" /> | |
215 <param name="factor_choice" value="No" /> | |
216 <param name="PCA_x_axis" value="2" /> | |
217 <param name="PCA_y_axis" value="3" /> | |
218 <output name="pdf_out" file="pca.2vs3.pdf" ftype="pdf"/> | |
219 </test> | |
220 | |
190 <!-- test Coordinates tables on PCA --> | 221 <!-- test Coordinates tables on PCA --> |
191 <test> | 222 <test> |
192 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 223 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
193 <param name="labels" value="no" /> | 224 <param name="labels" value="no" /> |
194 <param name="visu_choice" value="PCA" /> | 225 <param name="visu_choice" value="PCA" /> |
211 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 242 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
212 <param name="labels" value="no" /> | 243 <param name="labels" value="no" /> |
213 <param name="visu_choice" value="PCA" /> | 244 <param name="visu_choice" value="PCA" /> |
214 <param name="factor_choice" value="Yes" /> | 245 <param name="factor_choice" value="Yes" /> |
215 <param name="factor" value="2-lev_factor.tsv" ftype="txt"/> | 246 <param name="factor" value="2-lev_factor.tsv" ftype="txt"/> |
216 <output name="pdf_out" file="pca.nolabels.2-lev-factor.pdf" ftype="pdf"/> | 247 <output name="pdf_out" file="pca.nolabels.2-lev-factor.pdf" compare="sim_size" ftype="pdf"/> |
248 </test> | |
249 <!-- test numerical factor contrasting on PCA --> | |
250 <test> | |
251 <param name="input" value="cpm_input.tsv" ftype="txt"/> | |
252 <param name="labels" value="no" /> | |
253 <param name="visu_choice" value="PCA" /> | |
254 <param name="factor_choice" value="Yes" /> | |
255 <param name="factor" value="numeric_factor.tsv" ftype="txt"/> | |
256 <output name="pdf_out" file="pca.nolabels.numerical-factor.pdf" compare="sim_size" ftype="pdf"/> | |
217 </test> | 257 </test> |
218 <test> | 258 <test> |
219 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 259 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
220 <param name="labels" value="no" /> | 260 <param name="labels" value="no" /> |
221 <param name="visu_choice" value="PCA" /> | 261 <param name="visu_choice" value="PCA" /> |
222 <param name="factor_choice" value="Yes" /> | 262 <param name="factor_choice" value="Yes" /> |
223 <param name="factor" value="shuffled_factor.tsv" ftype="txt"/> | 263 <param name="factor" value="shuffled_factor.tsv" ftype="txt"/> |
224 <output name="pdf_out" file="pca.nolabels.factors.pdf" ftype="pdf"/> | 264 <output name="pdf_out" file="pca.nolabels.factors.pdf" compare="sim_size" ftype="pdf"/> |
225 </test> | 265 </test> |
226 <!-- test HCPC --> | 266 <!-- test HCPC --> |
227 <test> | 267 <test> |
228 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 268 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
229 <param name="labels" value="yes" /> | 269 <param name="labels" value="yes" /> |
230 <param name="visu_choice" value="HCPC" /> | 270 <param name="visu_choice" value="HCPC" /> |
231 <param name="HCPC_npc" value="5"/> | 271 <param name="HCPC_npc" value="5"/> |
232 <param name="HCPC_ncluster" value="-1"/> | 272 <param name="HCPC_ncluster" value="-1"/> |
233 <output name="pdf_out" file="hcpc.labels.pdf" ftype="pdf"/> | 273 <output name="pdf_out" file="hcpc.labels.pdf" compare="sim_size" ftype="pdf"/> |
234 </test> | 274 </test> |
235 <!-- test factor contrasting on HCPC --> | 275 <!-- test factor contrasting on HCPC --> |
236 <test> | 276 <test> |
237 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 277 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
238 <param name="labels" value="no" /> | 278 <param name="labels" value="no" /> |
239 <param name="visu_choice" value="HCPC" /> | 279 <param name="visu_choice" value="HCPC" /> |
240 <param name="HCPC_npc" value="5"/> | 280 <param name="HCPC_npc" value="5"/> |
241 <param name="HCPC_ncluster" value="-1"/> | 281 <param name="HCPC_ncluster" value="-1"/> |
282 <param name="res_clustering" value="yes"/> | |
242 <param name="factor_choice" value="Yes" /> | 283 <param name="factor_choice" value="Yes" /> |
243 <param name="factor" value="factor.tsv" ftype="txt"/> | 284 <param name="factor" value="factor.tsv" ftype="txt"/> |
244 <output name="pdf_out" file="hcpc.nolabels.factor.pdf" ftype="pdf"/> | 285 <output name="pdf_out" file="hcpc.nolabels.factor.pdf" compare="sim_size" ftype="pdf"/> |
286 <output name="mutual_info" file="hcpc.factor.extval.txt" ftype="txt"/> | |
287 <output name="HCPC_clust" file="hcpc.clusters.tab" ftype="tabular"/> | |
245 </test> | 288 </test> |
246 <test> | 289 <test> |
247 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 290 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
248 <param name="labels" value="no" /> | 291 <param name="labels" value="no" /> |
249 <param name="HCPC_npc" value="5"/> | 292 <param name="HCPC_npc" value="5"/> |
250 <param name="HCPC_ncluster" value="-1"/> | 293 <param name="HCPC_ncluster" value="-1"/> |
251 <param name="visu_choice" value="HCPC" /> | 294 <param name="visu_choice" value="HCPC" /> |
252 <output name="pdf_out" file="hcpc.nolabels.pdf" ftype="pdf"/> | 295 <output name="pdf_out" file="hcpc.nolabels.pdf" compare="sim_size" ftype="pdf"/> |
253 </test> | 296 </test> |
254 <test> | 297 <test> |
255 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 298 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
256 <param name="labels" value="yes" /> | 299 <param name="labels" value="yes" /> |
257 <param name="visu_choice" value="HCPC" /> | 300 <param name="visu_choice" value="HCPC" /> |
269 <param name="coord" value="yes" /> | 312 <param name="coord" value="yes" /> |
270 <param name="HCPC_method" value="single"/> | 313 <param name="HCPC_method" value="single"/> |
271 <param name="HCPC_metric" value="euclidian"/> | 314 <param name="HCPC_metric" value="euclidian"/> |
272 <param name="HCPC_npc" value="4" /> | 315 <param name="HCPC_npc" value="4" /> |
273 <param name="HCPC_clusterCA" value="cols" /> | 316 <param name="HCPC_clusterCA" value="cols" /> |
274 <output name="pdf_out" file="hcpc-3.labels.pdf" ftype="pdf"/> | 317 <output name="pdf_out" file="hcpc-3.labels.pdf" compare="sim_size" ftype="pdf"/> |
275 <output name="table_coordinates" file="hcpc-3.coord.tab" ftype="tabular"/> | 318 <output name="table_coordinates" file="hcpc-3.coord.tab" ftype="tabular"/> |
276 </test> | 319 </test> |
277 <!-- test t-SNE --> | 320 <!-- test t-SNE --> |
278 <test> | 321 <test> |
279 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 322 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
280 <param name="labels" value="yes" /> | 323 <param name="labels" value="yes" /> |
281 <param name="visu_choice" value="tSNE" /> | 324 <param name="visu_choice" value="tSNE" /> |
282 <param name="Rtsne_seed" value="49"/> | 325 <param name="Rtsne_seed" value="49"/> |
283 <param name="Rtsne_perplexity" value="10"/> | 326 <param name="Rtsne_perplexity" value="10"/> |
284 <param name="Rtsne_theta" value="1" /> | 327 <param name="Rtsne_theta" value="1" /> |
285 <output name="pdf_out" file="tsne.labels.pdf" ftype="pdf"/> | 328 <output name="pdf_out" file="tsne.labels.pdf" ftype="pdf" compare="sim_size" delta="500"/> |
286 </test> | 329 </test> |
287 <test> | 330 <test> |
288 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 331 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
289 <param name="labels" value="no" /> | 332 <param name="labels" value="no" /> |
290 <param name="visu_choice" value="tSNE" /> | 333 <param name="visu_choice" value="tSNE" /> |
291 <param name="Rtsne_seed" value="49"/> | 334 <param name="Rtsne_seed" value="49"/> |
292 <param name="Rtsne_perplexity" value="10"/> | 335 <param name="Rtsne_perplexity" value="10"/> |
293 <param name="Rtsne_theta" value="1" /> | 336 <param name="Rtsne_theta" value="1" /> |
294 <output name="pdf_out" file="tsne.nolabels.pdf" ftype="pdf"/> | 337 <output name="pdf_out" file="tsne.nolabels.pdf" ftype="pdf" compare="sim_size" delta="500"/> |
295 </test> | 338 </test> |
296 <test> | 339 <!-- test factor contrasting on t-SNE --> |
297 <param name="input" value="cpm_input.tsv" ftype="txt"/> | |
298 <param name="labels" value="no" /> | |
299 <param name="visu_choice" value="tSNE" /> | |
300 <param name="coord" value="yes" /> | |
301 <param name="Rtsne_seed" value="42"/> | |
302 <param name="Rtsne_perplexity" value="5.0"/> | |
303 <param name="Rtsne_theta" value="1.0" /> | |
304 <param name="Rtsne_dims" value="3" /> | |
305 <param name="Rtsne_exaggeration_factor" value="15.0" /> | |
306 <output name="pdf_out" file="tsne-2.nolabels.pdf" ftype="pdf"/> | |
307 <output name="table_coordinates" file="tsne-2.coord.tab" ftype="tabular"/> | |
308 </test> | |
309 <!-- test factor contrasting on t-SNE --> | |
310 <test> | 340 <test> |
311 <param name="input" value="cpm_input.tsv" ftype="txt"/> | 341 <param name="input" value="cpm_input.tsv" ftype="txt"/> |
312 <param name="labels" value="yes" /> | 342 <param name="labels" value="yes" /> |
313 <param name="visu_choice" value="tSNE" /> | 343 <param name="visu_choice" value="tSNE" /> |
314 <param name="factor_choice" value="Yes" /> | 344 <param name="factor_choice" value="Yes" /> |
315 <param name="factor" value="shuffled_factor.tsv" ftype="txt"/> | 345 <param name="factor" value="shuffled_factor.tsv" ftype="txt"/> |
316 <param name="Rtsne_seed" value="49"/> | 346 <param name="Rtsne_seed" value="49"/> |
317 <param name="Rtsne_perplexity" value="10"/> | 347 <param name="Rtsne_perplexity" value="10"/> |
318 <param name="Rtsne_theta" value="1" /> | 348 <param name="Rtsne_theta" value="1" /> |
319 <output name="pdf_out" file="tsne.labels.factor.pdf" ftype="pdf"/> | 349 <output name="pdf_out" file="tsne.labels.factor.pdf" ftype="pdf" compare="sim_size" delta="500"/> |
350 </test> | |
351 <test> | |
352 <param name="input" value="cpm_input.tsv" ftype="txt"/> | |
353 <param name="labels" value="no" /> | |
354 <param name="visu_choice" value="tSNE" /> | |
355 <param name="Rtsne_seed" value="49" /> | |
356 <param name="coord" value="yes" /> | |
357 <param name="Rtsne_dims" value="3" /> | |
358 <param name="Rtsne_perplexity" value="10"/> | |
359 <param name="Rtsne_theta" value="1" /> | |
360 <param name="Rtsne_normalize" value="FALSE" /> | |
361 <output name="pdf_out" file="tsne-2.nolabels.pdf" ftype="pdf" compare="sim_size" delta="500"/> | |
362 <output name="table_coordinates" file="tsne-2.coord.tab" ftype="tabular" compare="sim_size" delta="500"/> | |
320 </test> | 363 </test> |
321 </tests> | 364 </tests> |
322 <help> | 365 <help> |
323 | 366 |
324 **What it does** | 367 **What it does** |
337 | 380 |
338 This tool returns the visualisation of a dimensional reduction using either: | 381 This tool returns the visualisation of a dimensional reduction using either: |
339 | 382 |
340 * Principal Components Analysis (PCA) | 383 * Principal Components Analysis (PCA) |
341 * Hierarchical Clustering of Principal Components (HCPC) | 384 * Hierarchical Clustering of Principal Components (HCPC) |
342 * t-distributed Stochastic Neighbor Embedding | 385 * t-distributed Stochastic Neighbor Embedding (t-SNE) |
343 | 386 |
344 The tool returns in addition the table of the coordinates of the observations (eg RNAseq libraries) | 387 The tool returns in addition the table of the coordinates of the observations (eg RNAseq libraries) |
345 in the low dim space, which can be used for post-treatment or to further adjust the provided visualisation. | 388 in the low dim space, which can be used for post-treatment or to further adjust the provided visualisation. |
389 | |
390 If HCPC is used, this tool can also return the clustering table. It contains two columns of n observations : | |
391 | |
392 * Observation labels | |
393 * Cluster labels | |
346 | 394 |
347 ** Contrast data with a factor ** | 395 ** Contrast data with a factor ** |
348 The tool offers the possibility to colour data points according to the levels of a factor. | 396 The tool offers the possibility to colour data points according to the levels of a factor. |
349 To use the option "Factor to contrast data", provide a tabulated-separated, two-column table | 397 To use the option "Factor to contrast data", provide a tabulated-separated, two-column table |
350 with first column containing the cell/data library identifiers (same identifiers as those | 398 with first column containing the cell/data library identifiers (same identifiers as those |
351 provided as column headers in the input data table) and second column containing the corresponding | 399 provided as column headers in the input data table) and second column containing the corresponding |
352 factor levels value. This table does not need to be sorted in the same order as in the data | 400 factor levels value (if this vector is numerical, then the color palette used is quantitative). |
401 This table does not need to be sorted in the same order as in the data | |
353 table. It may also contain more identifiers than those provided in the data table. | 402 table. It may also contain more identifiers than those provided in the data table. |
403 | |
404 If HCPC visualisation and constrasting data are chosen, an additional text file is given. It contains | |
405 several metrics of external validation of clustering. It will compare the capacity of HCPC clustering | |
406 to recreate classes contained in the factor data file. If the constrasting factor is quantitative, | |
407 the file will be empty. | |
408 | |
354 | 409 |
355 </help> | 410 </help> |
356 <citations> | 411 <citations> |
357 <citation type="bibtex">@Article{, | 412 <citation type="bibtex">@Article{, |
358 title = {Visualizing High-Dimensional Data Using t-SNE}, | 413 title = {Visualizing High-Dimensional Data Using t-SNE}, |
379 year = {2015}, | 434 year = {2015}, |
380 note = {R package version 0.15}, | 435 note = {R package version 0.15}, |
381 url = {https://github.com/jkrijthe/Rtsne}, | 436 url = {https://github.com/jkrijthe/Rtsne}, |
382 } | 437 } |
383 </citation> | 438 </citation> |
439 <citation type="bibtex">@Manual{, | |
440 title = {{ClusterR}: Gaussian Mixture Models, K-Means, Mini-Batch-Kmeans, | |
441 K-Medoids and Affinity Propagation Clustering}, | |
442 author = {Lampros Mouselimis}, | |
443 year = {2019}, | |
444 note = {R package version 1.1.9}, | |
445 url = {https://github.com/mlampros/ClusterR}, | |
446 } | |
447 </citation> | |
384 </citations> | 448 </citations> |
385 </tool> | 449 </tool> |