changeset 14:c163574c246f draft

"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mircounts commit 51dc6c56c7d95fc229ffee958354211cd454fd36"
author artbio
date Sun, 09 May 2021 17:10:07 +0000
parents b045c30fb768
children ffcd42f85b61
files coverage_plotting.R mircounts.xml test-data/aga.22.gff3 test-data/aga.bam test-data/aga_lattice_dataframe.tab test-data/aga_mir_coverage.pdf test-data/clipped.out.bam test-data/lattice_clipped_dataframe.tab test-data/lattice_unclipped_dataframe.tab test-data/mir_clipped_coverage.pdf test-data/mir_unclipped_coverage.pdf test-data/mouse.19.gff3 test-data/mouse.bam test-data/mouse_lattice_dataframe.tab test-data/mouse_mir_coverage.pdf test-data/translated_dme.22.gff3 test-data/translated_dme.gff3 test-data/unclipped.out.22.bam test-data/unclipped.out.bam
diffstat 19 files changed, 118119 insertions(+), 118115 deletions(-) [+]
line wrap: on
line diff
--- a/coverage_plotting.R	Fri Oct 18 19:18:50 2019 -0400
+++ b/coverage_plotting.R	Sun May 09 17:10:07 2021 +0000
@@ -1,53 +1,56 @@
 #!/usr/bin/env Rscript
 
 # Help to be printed
-hlp_description = "This script takes a dataframe containing the coverage values for different miRNAs and plots them."
-hlp_usage = "Usage : coverage_ploting.R --dataframe [FILE] --type ['relative' or 'absolute'] --output [FILE]"
-hlp_dataframe = "--dataframe\tFILE\tThis is a dataframe containing coverage values obtained from mircounts.py"
-hlp_type = "--type\t\tSTRING\tType of ploting, either relative or absoute coverage values (default='relative')"
-hlp_output = "--output\tFILE\tFile to output the pdf to\n"
+hlp_description <- "This script takes a dataframe containing the coverage values for different miRNAs and plots them."
+hlp_usage <- "Usage : coverage_ploting.R --dataframe [FILE] --type ['relative' or 'absolute'] --output [FILE]"
+hlp_dataframe <- "--dataframe\tFILE\tThis is a dataframe containing coverage values obtained from mircounts.py"
+hlp_type <- "--type\t\tSTRING\tType of ploting, either relative or absoute coverage values (default='relative')"
+hlp_output <- "--output\tFILE\tFile to output the pdf to\n"
 
-hlp = paste(hlp_description,hlp_usage,hlp_dataframe,hlp_type,hlp_output,sep="\n")
-#print(hlp)
+hlp <- paste(hlp_description, hlp_usage, hlp_dataframe, hlp_type, hlp_output, sep = "\n")
 
 # Setup R error handling to go to stderr
-options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } )
+options(show.error.messages = F,
+    error = function() {
+        cat(geterrmessage(), file = stderr()); q("no", 1, F)
+    }
+)
 
 library(optparse)
 library(lattice)
 
 # Get arguments
 option_list <- list(
-    make_option(c("-d", "--dataframe"), type="character",
-                help="Dataframe containing coverage values obtained from mircounts.py"),
-    make_option(c("-t", "--type"), type="character", default="relative",
-                help="Type of plotting, either relative or absoute coverage values (default='relative')"),
-    make_option(c("-o", "--output"), type="character", help="File to output the pdf to")
+    make_option(c("-d", "--dataframe"), type = "character",
+                help = "Dataframe containing coverage values obtained from mircounts.py"),
+    make_option(c("-t", "--type"), type = "character", default = "relative",
+                help = "Type of plotting, either relative or absoute coverage values (default = 'relative')"),
+    make_option(c("-o", "--output"), type = "character", help = "File to output the pdf to")
     )
-parser <- OptionParser(usage = "%prog [options] file", option_list=option_list)
-args = parse_args(parser)
+parser <- OptionParser(usage = "%prog [options] file", option_list = option_list)
+args <- parse_args(parser)
 
-if ( !('dataframe' %in% names(args)) || !('output' %in% names(args))) {
+if (!("dataframe" %in% names(args)) || !("output" %in% names(args))) {
     stop("'--dataframe' and '--output' parametters are not optional. Please retry.")
 }
 
 # Plot
-coverage = read.delim(args$dataframe, header=T)
-if (args$type =="relative") {
-    graph = xyplot(Norm_count~Norm_offset | Mir_hairpin, data=coverage, col=c("darkblue"), type="l", lwd=1.5,
-                   scales=list(x=list(cex=.5), y=list(cex=.5)), par.strip.text=list(cex=.5),
-                   strip=strip.custom(which.given=1, bg="lightblue"), layout=c(4,15),
-                   as.table=T, xlab="Normalized Counts", ylab="Normalized coordinates",
-                   main="miRNA coverage maps")
+coverage <- read.delim(args$dataframe, header = T)
+if (args$type == "relative") {
+    graph <- xyplot(Norm_count ~ Norm_offset | Mir_hairpin, data = coverage, col = c("darkblue"), type = "l", lwd = 1.5,
+                   scales = list(x = list(cex = .5), y = list(cex = .5)), par.strip.text = list(cex = .5),
+                   strip = strip.custom(which.given = 1, bg = "lightblue"), layout = c(4, 15),
+                   as.table = T, xlab = "Normalized Counts", ylab = "Normalized coordinates",
+                   main = "miRNA coverage maps")
 } else {
-    graph = xyplot(Count~Offset | Mir_hairpin, data=coverage, col=c("darkblue"), type="l", lwd=1.5,
-                   scales=list(x=list(cex=.5), y=list(cex=.5)), par.strip.text=list(cex=.5),
-                   strip=strip.custom(which.given=1, bg="lightblue"), layout=c(4,15),
-                   as.table=T, xlab="Counts", ylab="Coordinates",
-                   main="miRNA coverage plots")
+    graph <- xyplot(Count ~ Offset | Mir_hairpin, data = coverage, col = c("darkblue"), type = "l", lwd = 1.5,
+                   scales = list(x = list(cex = .5), y = list(cex = .5)), par.strip.text = list(cex = .5),
+                   strip = strip.custom(which.given = 1, bg = "lightblue"), layout = c(4, 15),
+                   as.table = T, xlab = "Counts", ylab = "Coordinates",
+                   main = "miRNA coverage plots")
 }
 
 # PDF output
-pdf(file=args$output, paper="special", height=11.69, width=8.2677)
+pdf(file = args$output, paper = "special", height = 11.69, width = 8.2677)
 plot(graph, newpage = T)
 dev.off()
--- a/mircounts.xml	Fri Oct 18 19:18:50 2019 -0400
+++ b/mircounts.xml	Sun May 09 17:10:07 2021 +0000
@@ -1,17 +1,18 @@
-<tool id="mircounts" name="miRcounts" version="1.4.0">
+<tool id="mircounts" name="miRcounts" version="1.5.1">
     <description> Counts miRNA alignments from small RNA sequence data</description>
     <requirements>
-        <requirement type="package" version="1.2.0">bowtie</requirement>
-        <requirement type="package" version="1.9=h10a08f8_12">samtools</requirement>
-        <requirement type="package" version="0.15.3=py27hda2845c_1">pysam</requirement>
-        <requirement type="package" version="1.6.4=r36h6115d3f_0">r-optparse</requirement>
-        <requirement type="package" version="0.20_38=r36hcdcec82_1002">r-lattice</requirement>
+        <requirement type="package" version="1.34=ha1f6473_0">tar</requirement>
+        <requirement type="package" version="1.3.0=py39h176da8b_2">bowtie</requirement>
+        <requirement type="package" version="1.12=h9aed4be_1">samtools</requirement>
+        <requirement type="package" version="0.16.0.1=py39h051187c_3">pysam</requirement>
+        <requirement type="package" version="1.6.6=r40h6115d3f_1">r-optparse</requirement>
+        <requirement type="package" version="0.20_44=r40hcfec24a_0">r-lattice</requirement>
     </requirements>
     <stdio>
         <exit_code range="1:" level="warning" description="Tool exception" />
     </stdio>
     <command detect_errors="exit_code"><![CDATA[
-        tar -xvzf  '$__tool_directory__'/mirbase.tar.gz &&
+        tar -xf  '$__tool_directory__'/mirbase.tar.gz &&
         python '$__tool_directory__'/mature_mir_gff_translation.py
                                                 --gff_path mirbase/${mirbase_version}/genomes/${genomeKey}.gff3
                                                 --output $gff3 && ## transcode the mature miR genome coordinates into coordinates relative to the corresponding "miRNA_primary_transcript".
@@ -144,12 +145,12 @@
             <param name="display" value="relative"/>
             <param name="output_premir_counts" value="True"/>
             <param name="output_mir_counts" value="True"/>
-            <output name="output" file="aga.bam"/>
-            <output name="gff3" file="aga.22.gff3" lines_diff="22"/>
-            <output name="pre_mir_count_file" file="aga_pre_mirs_count.22.tab"/>
-            <output name="mir_count_file" file="aga_mirs_count.22.tab"/>
-            <output name="latticePDF" file="aga_mir_coverage.pdf"/>
-            <output name="coverage_dataframe" file="aga_lattice_dataframe.tab"/>
+            <output name="output" file="aga.bam" compare="sim_size" />
+            <output name="gff3" file="aga.22.gff3" lines_diff="22" ftype="gff3" />
+            <output name="pre_mir_count_file" file="aga_pre_mirs_count.22.tab" ftype="tabular" />
+            <output name="mir_count_file" file="aga_mirs_count.22.tab" ftype="tabular" />
+            <output name="latticePDF" file="aga_mir_coverage.pdf" ftype="pdf" />
+            <output name="coverage_dataframe" file="aga_lattice_dataframe.tab" ftype="tabular" />
         </test>
         <test>
             <param name="cutoption" value="yes" />
@@ -165,12 +166,12 @@
             <param name="display" value="relative"/>
             <param name="output_premir_counts" value="True"/>
             <param name="output_mir_counts" value="True"/>
-            <output name="output" file="mouse.bam"/>
-            <output name="gff3" file="mouse.19.gff3" lines_diff="22"/>
-            <output name="pre_mir_count_file" file="mouse_pre_mirs_count.29.tab"/>
-            <output name="mir_count_file" file="mouse_mirs_count.29.tab"/>
-            <output name="latticePDF" file="mouse_mir_coverage.pdf"/>
-            <output name="coverage_dataframe" file="mouse_lattice_dataframe.tab"/>
+            <output name="output" file="mouse.bam" compare="sim_size"/>
+            <output name="gff3" file="mouse.19.gff3" lines_diff="22" ftype="gff3" />
+            <output name="pre_mir_count_file" file="mouse_pre_mirs_count.29.tab" ftype="tabular" />
+            <output name="mir_count_file" file="mouse_mirs_count.29.tab" ftype="tabular" />
+            <output name="latticePDF" file="mouse_mir_coverage.pdf" ftype="pdf" />
+            <output name="coverage_dataframe" file="mouse_lattice_dataframe.tab" ftype="tabular" />
         </test>
         <test>
             <param name="cutoption" value="yes" />
@@ -185,10 +186,10 @@
             <param name="plottingOption" value="no"/>
             <param name="output_premir_counts" value="True"/>
             <param name="output_mir_counts" value="True"/>
-            <output name="output" file="unclipped.out.22.bam"/>
-            <output name="gff3" file="translated_dme.22.gff3" lines_diff="22"/>
-            <output name="pre_mir_count_file" file="pre_mirs_unclipped_count.22.tab"/>
-            <output name="mir_count_file" file="mirs_unclipped_count.22.tab"/>
+            <output name="output" file="unclipped.out.22.bam" compare="sim_size" />
+            <output name="gff3" file="translated_dme.22.gff3" lines_diff="22" ftype="gff3" />
+            <output name="pre_mir_count_file" file="pre_mirs_unclipped_count.22.tab" ftype="tabular" />
+            <output name="mir_count_file" file="mirs_unclipped_count.22.tab" ftype="tabular" />
         </test>
          <test>
             <param name="cutoption" value="yes" />
@@ -203,10 +204,10 @@
             <param name="plottingOption" value="no"/>
             <param name="output_premir_counts" value="True"/>
             <param name="output_mir_counts" value="True"/>
-            <output name="output" file="unclipped.out.bam" />
-            <output name="gff3" file="translated_dme.gff3" lines_diff="22"/>
-            <output name="pre_mir_count_file" file="pre_mirs_unclipped_count.tab"/>
-            <output name="mir_count_file" file="mirs_unclipped_count.tab"/>
+            <output name="output" file="unclipped.out.bam" compare="sim_size" />
+            <output name="gff3" file="translated_dme.gff3" lines_diff="22"  ftype="gff3" />
+            <output name="pre_mir_count_file" file="pre_mirs_unclipped_count.tab" ftype="tabular" />
+            <output name="mir_count_file" file="mirs_unclipped_count.tab" ftype="tabular" />
         </test>
         <test>
             <param name="cutoption" value="yes" />
@@ -222,12 +223,12 @@
             <param name="display" value="relative"/>
             <param name="output_premir_counts" value="True"/>
             <param name="output_mir_counts" value="True"/>
-            <output name="output" file="unclipped.out.bam"/>
-            <output name="gff3" file="translated_dme.gff3"  lines_diff="22"/>
-            <output name="pre_mir_count_file" file="pre_mirs_unclipped_count.tab"/>
-            <output name="mir_count_file" file="mirs_unclipped_count.tab"/>
-            <output name="latticePDF" file="mir_unclipped_coverage.pdf"/>
-            <output name="coverage_dataframe" file="lattice_unclipped_dataframe.tab"/>
+            <output name="output" file="unclipped.out.bam" compare="sim_size" />
+            <output name="gff3" file="translated_dme.gff3"  lines_diff="22" ftype="gff3" />
+            <output name="pre_mir_count_file" file="pre_mirs_unclipped_count.tab" ftype="tabular" />
+            <output name="mir_count_file" file="mirs_unclipped_count.tab" ftype="tabular" />
+            <output name="latticePDF" file="mir_unclipped_coverage.pdf" ftype="pdf"/>
+            <output name="coverage_dataframe" file="lattice_unclipped_dataframe.tab" ftype="tabular" />
         </test>
         <test>
             <param name="cutoption" value="no" />
@@ -239,12 +240,12 @@
             <param name="display" value="absolute"/>
             <param name="output_premir_counts" value="True"/>
             <param name="output_mir_counts" value="True"/>
-            <output name="output" file="clipped.out.bam"/>
-            <output name="gff3" file="translated_dme.gff3" lines_diff="22"/>
-            <output name="pre_mir_count_file" file="pre_mirs_clipped_count.tab"/>
-            <output name="mir_count_file" file="mirs_clipped_count.tab"/>
-            <output name="latticePDF" file="mir_clipped_coverage.pdf"/>
-            <output name="coverage_dataframe" file="lattice_clipped_dataframe.tab"/>
+            <output name="output" file="clipped.out.bam" compare="sim_size" />
+            <output name="gff3" file="translated_dme.gff3" lines_diff="22" ftype="gff3" />
+            <output name="pre_mir_count_file" file="pre_mirs_clipped_count.tab" ftype="tabular" />
+            <output name="mir_count_file" file="mirs_clipped_count.tab" ftype="tabular" />
+            <output name="latticePDF" file="mir_clipped_coverage.pdf" ftype="pdf" />
+            <output name="coverage_dataframe" file="lattice_clipped_dataframe.tab" ftype="tabular" />
         </test>
     </tests>
     <help>
--- a/test-data/aga.22.gff3	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/aga.22.gff3	Sun May 09 17:10:07 2021 +0000
@@ -11,7 +11,7 @@
 # rather a predicted stem-loop portion that includes the precursor 
 # miRNA. Mature sequences have type "miRNA".
 #
-# generated by mature_mir_gff_translation.py                             2019-10-18 20:28:58.142506
+# generated by mature_mir_gff_translation.py                             2021-05-09 00:34:02.737449
 #
 aga-bantam	.	miRNA_primary_transcript	1	111	.	+	.	ID=MI0001599;Alias=MI0001599;Name=aga-bantam
 aga-bantam	.	miRNA	70	92	.	+	.	ID=MIMAT0001494;Alias=MIMAT0001494;Name=aga-bantam;Derives_from=MI0001599;Parent_mir_Name=aga-bantam
Binary file test-data/aga.bam has changed
--- a/test-data/aga_lattice_dataframe.tab	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/aga_lattice_dataframe.tab	Sun May 09 17:10:07 2021 +0000
@@ -1,803 +1,803 @@
 Mir_hairpin	Offset	Norm_offset	Count	Norm_count
-aga-bantam	1	0.00900900900901	0.0	0.0
-aga-bantam	2	0.018018018018	0.0	0.0
-aga-bantam	3	0.027027027027	0.0	0.0
-aga-bantam	4	0.036036036036	0.0	0.0
-aga-bantam	5	0.045045045045	0.0	0.0
-aga-bantam	6	0.0540540540541	0.0	0.0
-aga-bantam	7	0.0630630630631	0.0	0.0
-aga-bantam	8	0.0720720720721	0.0	0.0
-aga-bantam	9	0.0810810810811	0.0	0.0
-aga-bantam	10	0.0900900900901	0.0	0.0
-aga-bantam	11	0.0990990990991	0.0	0.0
-aga-bantam	12	0.108108108108	0.0	0.0
-aga-bantam	13	0.117117117117	0.0	0.0
-aga-bantam	14	0.126126126126	0.0	0.0
-aga-bantam	15	0.135135135135	0.0	0.0
-aga-bantam	16	0.144144144144	0.0	0.0
-aga-bantam	17	0.153153153153	0.0	0.0
-aga-bantam	18	0.162162162162	0.0	0.0
-aga-bantam	19	0.171171171171	0.0	0.0
-aga-bantam	20	0.18018018018	0.0	0.0
-aga-bantam	21	0.189189189189	0.0	0.0
-aga-bantam	22	0.198198198198	0.0	0.0
-aga-bantam	23	0.207207207207	0.0	0.0
-aga-bantam	24	0.216216216216	0.0	0.0
-aga-bantam	25	0.225225225225	0.0	0.0
-aga-bantam	26	0.234234234234	0.0	0.0
-aga-bantam	27	0.243243243243	157.0	0.0172888448409
-aga-bantam	28	0.252252252252	177.0	0.0194912454575
-aga-bantam	29	0.261261261261	190.0	0.0209228058584
-aga-bantam	30	0.27027027027	190.0	0.0209228058584
-aga-bantam	31	0.279279279279	190.0	0.0209228058584
-aga-bantam	32	0.288288288288	190.0	0.0209228058584
-aga-bantam	33	0.297297297297	190.0	0.0209228058584
-aga-bantam	34	0.306306306306	190.0	0.0209228058584
-aga-bantam	35	0.315315315315	190.0	0.0209228058584
-aga-bantam	36	0.324324324324	190.0	0.0209228058584
-aga-bantam	37	0.333333333333	190.0	0.0209228058584
-aga-bantam	38	0.342342342342	190.0	0.0209228058584
-aga-bantam	39	0.351351351351	190.0	0.0209228058584
-aga-bantam	40	0.36036036036	190.0	0.0209228058584
-aga-bantam	41	0.369369369369	190.0	0.0209228058584
-aga-bantam	42	0.378378378378	190.0	0.0209228058584
-aga-bantam	43	0.387387387387	190.0	0.0209228058584
-aga-bantam	44	0.396396396396	190.0	0.0209228058584
-aga-bantam	45	0.405405405405	190.0	0.0209228058584
-aga-bantam	46	0.414414414414	190.0	0.0209228058584
-aga-bantam	47	0.423423423423	190.0	0.0209228058584
-aga-bantam	48	0.432432432432	189.0	0.0208126858276
-aga-bantam	49	0.441441441441	180.0	0.01982160555
-aga-bantam	50	0.45045045045	159.0	0.0175090849025
-aga-bantam	51	0.459459459459	0.0	0.0
-aga-bantam	52	0.468468468468	0.0	0.0
-aga-bantam	53	0.477477477477	0.0	0.0
-aga-bantam	54	0.486486486486	0.0	0.0
-aga-bantam	55	0.495495495495	0.0	0.0
-aga-bantam	56	0.504504504505	0.0	0.0
-aga-bantam	57	0.513513513514	0.0	0.0
-aga-bantam	58	0.522522522523	0.0	0.0
-aga-bantam	59	0.531531531532	0.0	0.0
-aga-bantam	60	0.540540540541	0.0	0.0
-aga-bantam	61	0.54954954955	0.0	0.0
-aga-bantam	62	0.558558558559	0.0	0.0
-aga-bantam	63	0.567567567568	0.0	0.0
-aga-bantam	64	0.576576576577	0.0	0.0
-aga-bantam	65	0.585585585586	0.0	0.0
-aga-bantam	66	0.594594594595	0.0	0.0
-aga-bantam	67	0.603603603604	0.0	0.0
-aga-bantam	68	0.612612612613	0.0	0.0
-aga-bantam	69	0.621621621622	11.0	0.00121132033917
-aga-bantam	70	0.630630630631	9079.0	0.999779759938
-aga-bantam	71	0.63963963964	9080.0	0.999889879969
-aga-bantam	72	0.648648648649	9080.0	0.999889879969
-aga-bantam	73	0.657657657658	9080.0	0.999889879969
-aga-bantam	74	0.666666666667	9080.0	0.999889879969
-aga-bantam	75	0.675675675676	9080.0	0.999889879969
-aga-bantam	76	0.684684684685	9080.0	0.999889879969
-aga-bantam	77	0.693693693694	9081.0	1.0
-aga-bantam	78	0.702702702703	9081.0	1.0
-aga-bantam	79	0.711711711712	9081.0	1.0
-aga-bantam	80	0.720720720721	9081.0	1.0
-aga-bantam	81	0.72972972973	9081.0	1.0
-aga-bantam	82	0.738738738739	9081.0	1.0
-aga-bantam	83	0.747747747748	9081.0	1.0
-aga-bantam	84	0.756756756757	9081.0	1.0
-aga-bantam	85	0.765765765766	9078.0	0.999669639907
-aga-bantam	86	0.774774774775	9068.0	0.998568439599
-aga-bantam	87	0.783783783784	9059.0	0.997577359322
-aga-bantam	88	0.792792792793	8989.0	0.989868957163
-aga-bantam	89	0.801801801802	8907.0	0.980839114635
-aga-bantam	90	0.810810810811	7590.0	0.835811034027
-aga-bantam	91	0.81981981982	5925.0	0.652461182689
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+aga-mir-iab-4	69	0.8214285714285714	0.0	0.0
+aga-mir-iab-4	70	0.8333333333333334	0.0	0.0
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+aga-mir-iab-4	83	0.9880952380952381	0.0	0.0
 aga-mir-iab-4	84	1.0	0.0	0.0
Binary file test-data/aga_mir_coverage.pdf has changed
Binary file test-data/clipped.out.bam has changed
--- a/test-data/lattice_clipped_dataframe.tab	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/lattice_clipped_dataframe.tab	Sun May 09 17:10:07 2021 +0000
@@ -1,330 +1,330 @@
 Mir_hairpin	Offset	Norm_offset	Count	Norm_count
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 dme-mir-iab-4	68	1.0	0.0	0
-dme-mir-iab-8	1	0.0147058823529	0.0	0
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 dme-mir-iab-8	17	0.25	0.0	0
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 dme-mir-iab-8	34	0.5	0.0	0
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 dme-mir-iab-8	68	1.0	0.0	0
--- a/test-data/lattice_unclipped_dataframe.tab	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/lattice_unclipped_dataframe.tab	Sun May 09 17:10:07 2021 +0000
@@ -1,330 +1,330 @@
 Mir_hairpin	Offset	Norm_offset	Count	Norm_count
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-dme-bantam	6	0.0740740740741	0.0	0.0
-dme-bantam	7	0.0864197530864	0.0	0.0
-dme-bantam	8	0.0987654320988	0.0	0.0
-dme-bantam	9	0.111111111111	0.0	0.0
-dme-bantam	10	0.123456790123	0.0	0.0
-dme-bantam	11	0.135802469136	0.0	0.0
-dme-bantam	12	0.148148148148	0.0	0.0
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-dme-bantam	15	0.185185185185	0.0	0.0
-dme-bantam	16	0.197530864198	0.0	0.0
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-dme-bantam	18	0.222222222222	0.0	0.0
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 dme-mir-iab-8	68	1.0	0.0	0
Binary file test-data/mir_clipped_coverage.pdf has changed
Binary file test-data/mir_unclipped_coverage.pdf has changed
--- a/test-data/mouse.19.gff3	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/mouse.19.gff3	Sun May 09 17:10:07 2021 +0000
@@ -11,7 +11,7 @@
 # rather a predicted stem-loop portion that includes the precursor 
 # miRNA. Mature sequences have type "miRNA".
 #
-# generated by mature_mir_gff_translation.py                             2019-10-18 20:30:06.659497
+# generated by mature_mir_gff_translation.py                             2021-05-09 00:34:28.737636
 #
 mmu-let-7a-1	.	miRNA_primary_transcript	1	94	.	-	.	ID=MI0000556;accession_number=MI0000556;Name=mmu-let-7a-1
 mmu-let-7a-5p	.	miRNA	61	82	.	-	.	ID=MIMAT0000521;accession_number=MIMAT0000521;Name=mmu-let-7a-5p;derives_from=MI0000556;Parent_mir_Name=mmu-let-7a-1
Binary file test-data/mouse.bam has changed
--- a/test-data/mouse_lattice_dataframe.tab	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/mouse_lattice_dataframe.tab	Sun May 09 17:10:07 2021 +0000
@@ -1,1240 +1,1240 @@
 Mir_hairpin	Offset	Norm_offset	Count	Norm_count
-mmu-let-7a-1	1	0.0106382978723	0.0	0.0
-mmu-let-7a-1	2	0.0212765957447	0.0	0.0
-mmu-let-7a-1	3	0.031914893617	0.0	0.0
-mmu-let-7a-1	4	0.0425531914894	0.0	0.0
-mmu-let-7a-1	5	0.0531914893617	0.0	0.0
-mmu-let-7a-1	6	0.063829787234	0.0	0.0
-mmu-let-7a-1	7	0.0744680851064	0.0	0.0
-mmu-let-7a-1	8	0.0851063829787	0.0	0.0
-mmu-let-7a-1	9	0.0957446808511	0.0	0.0
-mmu-let-7a-1	10	0.106382978723	0.0	0.0
-mmu-let-7a-1	11	0.117021276596	0.0	0.0
-mmu-let-7a-1	12	0.127659574468	0.0	0.0
-mmu-let-7a-1	13	0.13829787234	8.0	1.0
-mmu-let-7a-1	14	0.148936170213	8.0	1.0
-mmu-let-7a-1	15	0.159574468085	8.0	1.0
-mmu-let-7a-1	16	0.170212765957	8.0	1.0
-mmu-let-7a-1	17	0.18085106383	8.0	1.0
-mmu-let-7a-1	18	0.191489361702	8.0	1.0
-mmu-let-7a-1	19	0.202127659574	8.0	1.0
-mmu-let-7a-1	20	0.212765957447	8.0	1.0
-mmu-let-7a-1	21	0.223404255319	8.0	1.0
-mmu-let-7a-1	22	0.234042553191	8.0	1.0
-mmu-let-7a-1	23	0.244680851064	8.0	1.0
-mmu-let-7a-1	24	0.255319148936	8.0	1.0
-mmu-let-7a-1	25	0.265957446809	8.0	1.0
-mmu-let-7a-1	26	0.276595744681	8.0	1.0
-mmu-let-7a-1	27	0.287234042553	8.0	1.0
-mmu-let-7a-1	28	0.297872340426	8.0	1.0
-mmu-let-7a-1	29	0.308510638298	8.0	1.0
-mmu-let-7a-1	30	0.31914893617	8.0	1.0
-mmu-let-7a-1	31	0.329787234043	8.0	1.0
-mmu-let-7a-1	32	0.340425531915	8.0	1.0
-mmu-let-7a-1	33	0.351063829787	8.0	1.0
-mmu-let-7a-1	34	0.36170212766	7.0	0.875
-mmu-let-7a-1	35	0.372340425532	1.0	0.125
-mmu-let-7a-1	36	0.382978723404	0.0	0.0
-mmu-let-7a-1	37	0.393617021277	0.0	0.0
-mmu-let-7a-1	38	0.404255319149	0.0	0.0
-mmu-let-7a-1	39	0.414893617021	0.0	0.0
-mmu-let-7a-1	40	0.425531914894	0.0	0.0
-mmu-let-7a-1	41	0.436170212766	0.0	0.0
-mmu-let-7a-1	42	0.446808510638	0.0	0.0
-mmu-let-7a-1	43	0.457446808511	0.0	0.0
-mmu-let-7a-1	44	0.468085106383	0.0	0.0
-mmu-let-7a-1	45	0.478723404255	0.0	0.0
-mmu-let-7a-1	46	0.489361702128	0.0	0.0
+mmu-let-7a-1	1	0.010638297872340425	0.0	0.0
+mmu-let-7a-1	2	0.02127659574468085	0.0	0.0
+mmu-let-7a-1	3	0.031914893617021274	0.0	0.0
+mmu-let-7a-1	4	0.0425531914893617	0.0	0.0
+mmu-let-7a-1	5	0.05319148936170213	0.0	0.0
+mmu-let-7a-1	6	0.06382978723404255	0.0	0.0
+mmu-let-7a-1	7	0.07446808510638298	0.0	0.0
+mmu-let-7a-1	8	0.0851063829787234	0.0	0.0
+mmu-let-7a-1	9	0.09574468085106383	0.0	0.0
+mmu-let-7a-1	10	0.10638297872340426	0.0	0.0
+mmu-let-7a-1	11	0.11702127659574468	0.0	0.0
+mmu-let-7a-1	12	0.1276595744680851	0.0	0.0
+mmu-let-7a-1	13	0.13829787234042554	8.0	1.0
+mmu-let-7a-1	14	0.14893617021276595	8.0	1.0
+mmu-let-7a-1	15	0.1595744680851064	8.0	1.0
+mmu-let-7a-1	16	0.1702127659574468	8.0	1.0
+mmu-let-7a-1	17	0.18085106382978725	8.0	1.0
+mmu-let-7a-1	18	0.19148936170212766	8.0	1.0
+mmu-let-7a-1	19	0.20212765957446807	8.0	1.0
+mmu-let-7a-1	20	0.2127659574468085	8.0	1.0
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 mmu-let-7d	103	1.0	0.0	0.0
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 mmu-let-7f-1	89	1.0	0.0	0.0
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 mmu-let-7f-2	83	1.0	0.0	0.0
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 mmu-let-7g	11	0.125	3.0	1.0
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 mmu-let-7g	22	0.25	3.0	1.0
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 mmu-let-7g	33	0.375	0.0	0.0
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 mmu-let-7g	44	0.5	0.0	0.0
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 mmu-let-7g	55	0.625	0.0	0.0
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 mmu-let-7g	66	0.75	0.0	0.0
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 mmu-let-7g	77	0.875	0.0	0.0
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 mmu-let-7g	88	1.0	0.0	0.0
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 mmu-let-7i	17	0.2	53.0	1.0
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 mmu-let-7i	34	0.4	0.0	0.0
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 mmu-let-7i	51	0.6	0.0	0.0
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 mmu-let-7i	68	0.8	0.0	0.0
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 mmu-let-7k	110	1.0	0.0	0
 mmu-mir-100	1	0.0125	0.0	0.0
 mmu-mir-100	2	0.025	0.0	0.0
@@ -1316,432 +1316,432 @@
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 mmu-mir-100	79	0.9875	0.0	0.0
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 mmu-mir-374c	49	1.0	0.0	0
 mmu-mir-375	1	0.015625	0.0	0.0
 mmu-mir-375	2	0.03125	0.0	0.0
@@ -35591,1244 +35591,1244 @@
 mmu-mir-375	62	0.96875	0.0	0.0
 mmu-mir-375	63	0.984375	0.0	0.0
 mmu-mir-375	64	1.0	0.0	0.0
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+mmu-mir-99a	38	0.5846153846153846	0.0	0.0
 mmu-mir-99a	39	0.6	0.0	0.0
-mmu-mir-99a	40	0.615384615385	0.0	0.0
-mmu-mir-99a	41	0.630769230769	0.0	0.0
-mmu-mir-99a	42	0.646153846154	0.0	0.0
-mmu-mir-99a	43	0.661538461538	0.0	0.0
-mmu-mir-99a	44	0.676923076923	0.0	0.0
-mmu-mir-99a	45	0.692307692308	0.0	0.0
-mmu-mir-99a	46	0.707692307692	0.0	0.0
-mmu-mir-99a	47	0.723076923077	0.0	0.0
-mmu-mir-99a	48	0.738461538462	0.0	0.0
-mmu-mir-99a	49	0.753846153846	0.0	0.0
-mmu-mir-99a	50	0.769230769231	0.0	0.0
-mmu-mir-99a	51	0.784615384615	0.0	0.0
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+mmu-mir-99a	49	0.7538461538461538	0.0	0.0
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+mmu-mir-99a	51	0.7846153846153846	0.0	0.0
 mmu-mir-99a	52	0.8	0.0	0.0
-mmu-mir-99a	53	0.815384615385	0.0	0.0
-mmu-mir-99a	54	0.830769230769	0.0	0.0
-mmu-mir-99a	55	0.846153846154	0.0	0.0
-mmu-mir-99a	56	0.861538461538	0.0	0.0
-mmu-mir-99a	57	0.876923076923	0.0	0.0
-mmu-mir-99a	58	0.892307692308	0.0	0.0
-mmu-mir-99a	59	0.907692307692	0.0	0.0
-mmu-mir-99a	60	0.923076923077	0.0	0.0
-mmu-mir-99a	61	0.938461538462	0.0	0.0
-mmu-mir-99a	62	0.953846153846	0.0	0.0
-mmu-mir-99a	63	0.969230769231	0.0	0.0
-mmu-mir-99a	64	0.984615384615	0.0	0.0
+mmu-mir-99a	53	0.8153846153846154	0.0	0.0
+mmu-mir-99a	54	0.8307692307692308	0.0	0.0
+mmu-mir-99a	55	0.8461538461538461	0.0	0.0
+mmu-mir-99a	56	0.8615384615384616	0.0	0.0
+mmu-mir-99a	57	0.8769230769230769	0.0	0.0
+mmu-mir-99a	58	0.8923076923076924	0.0	0.0
+mmu-mir-99a	59	0.9076923076923077	0.0	0.0
+mmu-mir-99a	60	0.9230769230769231	0.0	0.0
+mmu-mir-99a	61	0.9384615384615385	0.0	0.0
+mmu-mir-99a	62	0.9538461538461539	0.0	0.0
+mmu-mir-99a	63	0.9692307692307692	0.0	0.0
+mmu-mir-99a	64	0.9846153846153847	0.0	0.0
 mmu-mir-99a	65	1.0	0.0	0.0
-mmu-mir-99b	1	0.0142857142857	0.0	0.0
-mmu-mir-99b	2	0.0285714285714	0.0	0.0
-mmu-mir-99b	3	0.0428571428571	0.0	0.0
-mmu-mir-99b	4	0.0571428571429	0.0	0.0
-mmu-mir-99b	5	0.0714285714286	0.0	0.0
-mmu-mir-99b	6	0.0857142857143	0.0	0.0
+mmu-mir-99b	1	0.014285714285714285	0.0	0.0
+mmu-mir-99b	2	0.02857142857142857	0.0	0.0
+mmu-mir-99b	3	0.04285714285714286	0.0	0.0
+mmu-mir-99b	4	0.05714285714285714	0.0	0.0
+mmu-mir-99b	5	0.07142857142857142	0.0	0.0
+mmu-mir-99b	6	0.08571428571428572	0.0	0.0
 mmu-mir-99b	7	0.1	36.0	1.0
-mmu-mir-99b	8	0.114285714286	36.0	1.0
-mmu-mir-99b	9	0.128571428571	36.0	1.0
-mmu-mir-99b	10	0.142857142857	36.0	1.0
-mmu-mir-99b	11	0.157142857143	36.0	1.0
-mmu-mir-99b	12	0.171428571429	36.0	1.0
-mmu-mir-99b	13	0.185714285714	36.0	1.0
-mmu-mir-99b	14	0.2	35.0	0.972222222222
-mmu-mir-99b	15	0.214285714286	36.0	1.0
-mmu-mir-99b	16	0.228571428571	36.0	1.0
-mmu-mir-99b	17	0.242857142857	36.0	1.0
-mmu-mir-99b	18	0.257142857143	36.0	1.0
-mmu-mir-99b	19	0.271428571429	36.0	1.0
-mmu-mir-99b	20	0.285714285714	36.0	1.0
+mmu-mir-99b	8	0.11428571428571428	36.0	1.0
+mmu-mir-99b	9	0.12857142857142856	36.0	1.0
+mmu-mir-99b	10	0.14285714285714285	36.0	1.0
+mmu-mir-99b	11	0.15714285714285714	36.0	1.0
+mmu-mir-99b	12	0.17142857142857143	36.0	1.0
+mmu-mir-99b	13	0.18571428571428572	36.0	1.0
+mmu-mir-99b	14	0.2	35.0	0.9722222222222222
+mmu-mir-99b	15	0.21428571428571427	36.0	1.0
+mmu-mir-99b	16	0.22857142857142856	36.0	1.0
+mmu-mir-99b	17	0.24285714285714285	36.0	1.0
+mmu-mir-99b	18	0.2571428571428571	36.0	1.0
+mmu-mir-99b	19	0.2714285714285714	36.0	1.0
+mmu-mir-99b	20	0.2857142857142857	36.0	1.0
 mmu-mir-99b	21	0.3	36.0	1.0
-mmu-mir-99b	22	0.314285714286	36.0	1.0
-mmu-mir-99b	23	0.328571428571	36.0	1.0
-mmu-mir-99b	24	0.342857142857	36.0	1.0
-mmu-mir-99b	25	0.357142857143	36.0	1.0
-mmu-mir-99b	26	0.371428571429	36.0	1.0
-mmu-mir-99b	27	0.385714285714	36.0	1.0
-mmu-mir-99b	28	0.4	29.0	0.805555555556
-mmu-mir-99b	29	0.414285714286	3.0	0.0833333333333
-mmu-mir-99b	30	0.428571428571	0.0	0.0
-mmu-mir-99b	31	0.442857142857	0.0	0.0
-mmu-mir-99b	32	0.457142857143	0.0	0.0
-mmu-mir-99b	33	0.471428571429	0.0	0.0
-mmu-mir-99b	34	0.485714285714	0.0	0.0
+mmu-mir-99b	22	0.3142857142857143	36.0	1.0
+mmu-mir-99b	23	0.32857142857142857	36.0	1.0
+mmu-mir-99b	24	0.34285714285714286	36.0	1.0
+mmu-mir-99b	25	0.35714285714285715	36.0	1.0
+mmu-mir-99b	26	0.37142857142857144	36.0	1.0
+mmu-mir-99b	27	0.38571428571428573	36.0	1.0
+mmu-mir-99b	28	0.4	29.0	0.8055555555555556
+mmu-mir-99b	29	0.4142857142857143	3.0	0.08333333333333333
+mmu-mir-99b	30	0.42857142857142855	0.0	0.0
+mmu-mir-99b	31	0.44285714285714284	0.0	0.0
+mmu-mir-99b	32	0.45714285714285713	0.0	0.0
+mmu-mir-99b	33	0.4714285714285714	0.0	0.0
+mmu-mir-99b	34	0.4857142857142857	0.0	0.0
 mmu-mir-99b	35	0.5	0.0	0.0
-mmu-mir-99b	36	0.514285714286	0.0	0.0
-mmu-mir-99b	37	0.528571428571	0.0	0.0
-mmu-mir-99b	38	0.542857142857	0.0	0.0
-mmu-mir-99b	39	0.557142857143	0.0	0.0
-mmu-mir-99b	40	0.571428571429	0.0	0.0
-mmu-mir-99b	41	0.585714285714	0.0	0.0
+mmu-mir-99b	36	0.5142857142857142	0.0	0.0
+mmu-mir-99b	37	0.5285714285714286	0.0	0.0
+mmu-mir-99b	38	0.5428571428571428	0.0	0.0
+mmu-mir-99b	39	0.5571428571428572	0.0	0.0
+mmu-mir-99b	40	0.5714285714285714	0.0	0.0
+mmu-mir-99b	41	0.5857142857142857	0.0	0.0
 mmu-mir-99b	42	0.6	0.0	0.0
-mmu-mir-99b	43	0.614285714286	0.0	0.0
-mmu-mir-99b	44	0.628571428571	0.0	0.0
-mmu-mir-99b	45	0.642857142857	0.0	0.0
-mmu-mir-99b	46	0.657142857143	0.0	0.0
-mmu-mir-99b	47	0.671428571429	0.0	0.0
-mmu-mir-99b	48	0.685714285714	0.0	0.0
+mmu-mir-99b	43	0.6142857142857143	0.0	0.0
+mmu-mir-99b	44	0.6285714285714286	0.0	0.0
+mmu-mir-99b	45	0.6428571428571429	0.0	0.0
+mmu-mir-99b	46	0.6571428571428571	0.0	0.0
+mmu-mir-99b	47	0.6714285714285714	0.0	0.0
+mmu-mir-99b	48	0.6857142857142857	0.0	0.0
 mmu-mir-99b	49	0.7	0.0	0.0
-mmu-mir-99b	50	0.714285714286	0.0	0.0
-mmu-mir-99b	51	0.728571428571	0.0	0.0
-mmu-mir-99b	52	0.742857142857	0.0	0.0
-mmu-mir-99b	53	0.757142857143	0.0	0.0
-mmu-mir-99b	54	0.771428571429	0.0	0.0
-mmu-mir-99b	55	0.785714285714	0.0	0.0
+mmu-mir-99b	50	0.7142857142857143	0.0	0.0
+mmu-mir-99b	51	0.7285714285714285	0.0	0.0
+mmu-mir-99b	52	0.7428571428571429	0.0	0.0
+mmu-mir-99b	53	0.7571428571428571	0.0	0.0
+mmu-mir-99b	54	0.7714285714285715	0.0	0.0
+mmu-mir-99b	55	0.7857142857142857	0.0	0.0
 mmu-mir-99b	56	0.8	0.0	0.0
-mmu-mir-99b	57	0.814285714286	0.0	0.0
-mmu-mir-99b	58	0.828571428571	0.0	0.0
-mmu-mir-99b	59	0.842857142857	0.0	0.0
-mmu-mir-99b	60	0.857142857143	0.0	0.0
-mmu-mir-99b	61	0.871428571429	0.0	0.0
-mmu-mir-99b	62	0.885714285714	0.0	0.0
+mmu-mir-99b	57	0.8142857142857143	0.0	0.0
+mmu-mir-99b	58	0.8285714285714286	0.0	0.0
+mmu-mir-99b	59	0.8428571428571429	0.0	0.0
+mmu-mir-99b	60	0.8571428571428571	0.0	0.0
+mmu-mir-99b	61	0.8714285714285714	0.0	0.0
+mmu-mir-99b	62	0.8857142857142857	0.0	0.0
 mmu-mir-99b	63	0.9	0.0	0.0
-mmu-mir-99b	64	0.914285714286	0.0	0.0
-mmu-mir-99b	65	0.928571428571	0.0	0.0
-mmu-mir-99b	66	0.942857142857	0.0	0.0
-mmu-mir-99b	67	0.957142857143	0.0	0.0
-mmu-mir-99b	68	0.971428571429	0.0	0.0
-mmu-mir-99b	69	0.985714285714	0.0	0.0
+mmu-mir-99b	64	0.9142857142857143	0.0	0.0
+mmu-mir-99b	65	0.9285714285714286	0.0	0.0
+mmu-mir-99b	66	0.9428571428571428	0.0	0.0
+mmu-mir-99b	67	0.9571428571428572	0.0	0.0
+mmu-mir-99b	68	0.9714285714285714	0.0	0.0
+mmu-mir-99b	69	0.9857142857142858	0.0	0.0
 mmu-mir-99b	70	1.0	0.0	0.0
Binary file test-data/mouse_mir_coverage.pdf has changed
--- a/test-data/translated_dme.22.gff3	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/translated_dme.22.gff3	Sun May 09 17:10:07 2021 +0000
@@ -11,7 +11,7 @@
 # rather a predicted stem-loop portion that includes the precursor 
 # miRNA. Mature sequences have type "miRNA".
 #
-# generated by mature_mir_gff_translation.py                             2019-10-18 20:31:17.146495
+# generated by mature_mir_gff_translation.py                             2021-05-09 00:35:07.306527
 #
 dme-bantam	.	miRNA_primary_transcript	1	81	.	+	.	ID=MI0000387;Alias=MI0000387;Name=dme-bantam
 dme-bantam-5p	.	miRNA	15	37	.	+	.	ID=MIMAT0020823;Alias=MIMAT0020823;Name=dme-bantam-5p;Derives_from=MI0000387;Parent_mir_Name=dme-bantam
--- a/test-data/translated_dme.gff3	Fri Oct 18 19:18:50 2019 -0400
+++ b/test-data/translated_dme.gff3	Sun May 09 17:10:07 2021 +0000
@@ -10,7 +10,7 @@
 # rather a predicted stem-loop portion that includes the precursor 
 # miRNA. Mature sequences have type "miRNA".
 #
-# generated by mature_mir_gff_translation.py                             2019-10-18 20:33:13.877649
+# generated by mature_mir_gff_translation.py                             2021-05-09 00:36:13.649865
 #
 dme-bantam	.	miRNA_primary_transcript	1	81	.	+	.	ID=MI0000387;Alias=MI0000387;Name=dme-bantam
 dme-bantam-5p	.	miRNA	15	37	.	+	.	ID=MIMAT0020823;Alias=MIMAT0020823;Name=dme-bantam-5p;Derives_from=MI0000387;Parent_mir_Name=dme-bantam
Binary file test-data/unclipped.out.22.bam has changed
Binary file test-data/unclipped.out.bam has changed