Mercurial > repos > artbio > mutational_patterns
annotate mutational_patterns.R @ 8:e0dad46148bf draft
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 75fd87e806f9bee2f6ff7fcd3834e55eb21d8710"
author | artbio |
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date | Mon, 19 Oct 2020 07:47:24 +0000 |
parents | 34626b2b9907 |
children | 4ff3c984523b |
rev | line source |
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0
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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1 # load packages that are provided in the conda env |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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2 options( show.error.messages=F, |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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3 error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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4 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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5 warnings() |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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6 library(optparse) |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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7 library(rjson) |
2
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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8 library(grid) |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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9 library(gridExtra) |
4
7ba08c826888
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e2d6ed12516e1bd24071962a0dfe0220cc348f3c"
artbio
parents:
3
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10 library(scales) |
7ba08c826888
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e2d6ed12516e1bd24071962a0dfe0220cc348f3c"
artbio
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3
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11 library(RColorBrewer) |
0
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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12 |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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13 # Arguments |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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14 option_list = list( |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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15 make_option( |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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16 "--inputs", |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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17 default = NA, |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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18 type = 'character', |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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19 help = "json formatted dictionary of datasets and their paths" |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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20 ), |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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21 make_option( |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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22 "--genome", |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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23 default = NA, |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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24 type = 'character', |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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25 help = "genome name in the BSgenome bioconductor package" |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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26 ), |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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27 make_option( |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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28 "--levels", |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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29 default = NA, |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
changeset
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30 type = 'character', |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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31 help = "path to the tab separated file describing the levels in function of datasets" |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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32 ), |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
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33 make_option( |
3
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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2
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34 "--cosmic_version", |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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2
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35 default = "v2", |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
parents:
2
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36 type = 'character', |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
parents:
2
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37 help = "Version of the Cosmic Signature set to be used to express mutational patterns" |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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2
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38 ), |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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2
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39 make_option( |
0
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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40 "--signum", |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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41 default = 2, |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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42 type = 'integer', |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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43 help = "selects the N most significant signatures in samples to express mutational patterns" |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
diff
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44 ), |
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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45 make_option( |
2
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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0
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46 "--nrun", |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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47 default = 2, |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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48 type = 'integer', |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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49 help = "Number of runs to fit signatures" |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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50 ), |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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51 make_option( |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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0
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52 "--rank", |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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0
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53 default = 2, |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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54 type = 'integer', |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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55 help = "number of ranks to display for parameter optimization" |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
diff
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56 ), |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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0
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57 make_option( |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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0
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58 "--newsignum", |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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59 default = 2, |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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60 type = 'integer', |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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61 help = "Number of new signatures to be captured" |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
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62 ), |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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63 make_option( |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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0
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64 "--output_spectrum", |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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65 default = NA, |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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66 type = 'character', |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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67 help = "path to output dataset" |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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68 ), |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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69 make_option( |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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70 "--output_denovo", |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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71 default = NA, |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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72 type = 'character', |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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73 help = "path to output dataset" |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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74 ), |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
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75 make_option( |
3
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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2
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76 "--sigmatrix", |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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2
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77 default = NA, |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
parents:
2
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78 type = 'character', |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
parents:
2
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79 help = "path to signature matrix" |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
parents:
2
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80 ), |
e332cf9dfa06
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit 0f7593f703ba0dfd12aea1c0460e371f08b57d2f"
artbio
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81 make_option( |
2
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
artbio
parents:
0
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82 "--output_cosmic", |
0
924c527fb379
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit e1f3ca871f13569401f41a5af9d0e281bf372540"
artbio
parents:
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83 default = NA, |
924c527fb379
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84 type = 'character', |
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85 help = "path to output dataset" |
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86 ), |
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87 make_option( |
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88 c("-r", "--rdata"), |
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89 type="character", |
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90 default=NULL, |
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91 help="Path to RData output file") |
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92 ) |
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93 |
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94 opt = parse_args(OptionParser(option_list = option_list), |
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95 args = commandArgs(trailingOnly = TRUE)) |
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96 |
2
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97 ################ Manage input data #################### |
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98 json_dict <- opt$inputs |
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99 parser <- newJSONParser() |
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100 parser$addData(json_dict) |
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101 fileslist <- parser$getObject() |
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102 vcf_paths <- attr(fileslist, "names") |
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103 element_identifiers <- unname(unlist(fileslist)) |
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104 ref_genome <- opt$genome |
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105 vcf_table <- data.frame(element_identifier=as.character(element_identifiers), path=vcf_paths) |
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106 |
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107 library(MutationalPatterns) |
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108 library(ref_genome, character.only = TRUE) |
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109 library(ggplot2) |
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110 |
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111 # Load the VCF files into a GRangesList: |
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112 vcfs <- read_vcfs_as_granges(vcf_paths, element_identifiers, ref_genome) |
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113 library(plyr) |
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114 if (!is.na(opt$levels)[1]) { # manage levels if there are |
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115 levels_table <- read.delim(opt$levels, header=FALSE, col.names=c("element_identifier","level")) |
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116 } else { |
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117 levels_table <- data.frame(element_identifier=vcf_table$element_identifier, |
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118 level=rep("nolabels", length(vcf_table$element_identifier))) |
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119 } |
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120 metadata_table <- join(vcf_table, levels_table, by = "element_identifier") |
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121 tissue <- as.vector(metadata_table$level) |
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122 detach(package:plyr) |
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123 |
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124 ##### This is done for any section ###### |
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125 mut_mat <- mut_matrix(vcf_list = vcfs, ref_genome = ref_genome) |
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126 qual_col_pals = brewer.pal.info[brewer.pal.info$category == 'qual',] |
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127 col_vector = unique(unlist(mapply(brewer.pal, qual_col_pals$maxcolors, rownames(qual_col_pals)))) |
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128 col_vector <- col_vector[c(-32, -34, -39)] # 67-color palette |
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129 |
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130 ###### Section 1 Mutation characteristics and spectrums ############# |
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131 if (!is.na(opt$output_spectrum)[1]) { |
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132 pdf(opt$output_spectrum, paper = "special", width = 11.69, height = 11.69) |
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133 type_occurrences <- mut_type_occurrences(vcfs, ref_genome) |
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134 |
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135 # mutation spectrum, total or by sample |
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136 |
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137 if (length(levels(factor(levels_table$level))) == 1) { # (is.na(opt$levels)[1]) |
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138 p1 <- plot_spectrum(type_occurrences, CT = TRUE, legend=TRUE) |
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139 plot(p1) |
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140 } else { |
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141 p2 <- plot_spectrum(type_occurrences, by = tissue, CT=TRUE) # by levels |
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142 p3 <- plot_spectrum(type_occurrences, CT=TRUE, legend=TRUE) # total |
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143 grid.arrange(p2, p3, ncol=2, widths=c(4,2.3), heights=c(4,1)) |
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144 } |
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145 plot_96_profile(mut_mat, condensed = TRUE) |
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146 dev.off() |
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147 } |
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148 |
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149 ###### Section 2: De novo mutational signature extraction using NMF ####### |
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150 if (!is.na(opt$output_denovo)[1]) { |
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151 # opt$rank cannot be higher than the number of samples |
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152 if (opt$rank > length(element_identifiers)) {opt$rank <-length(element_identifiers)} |
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153 # likewise, opt$signum cannot be higher thant the number of samples |
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154 if (opt$signum > length(element_identifiers)) {opt$signum <-length(element_identifiers)} |
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155 pseudo_mut_mat <- mut_mat + 0.0001 # First add a small pseudocount to the mutation count matrix |
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156 # Use the NMF package to generate an estimate rank plot |
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157 library("NMF") |
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158 estimate <- nmf(pseudo_mut_mat, rank=1:opt$rank, method="brunet", nrun=opt$nrun, seed=123456) |
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159 # And plot it |
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160 pdf(opt$output_denovo, paper = "special", width = 11.69, height = 11.69) |
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161 p4 <- plot(estimate) |
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162 grid.arrange(p4) |
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163 # Extract 4 (PARAMETIZE) mutational signatures from the mutation count matrix with extract_signatures |
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164 # (For larger datasets it is wise to perform more iterations by changing the nrun parameter |
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165 # to achieve stability and avoid local minima) |
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166 nmf_res <- extract_signatures(pseudo_mut_mat, rank=opt$newsignum, nrun=opt$nrun) |
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167 # Assign signature names |
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168 colnames(nmf_res$signatures) <- paste0("NewSig_", 1:opt$newsignum) |
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169 rownames(nmf_res$contribution) <- paste0("NewSig_", 1:opt$newsignum) |
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170 # Plot the 96-profile of the signatures: |
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171 p5 <- plot_96_profile(nmf_res$signatures, condensed = TRUE) |
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172 new_sig_matrix <- reshape2::dcast(p5$data, substitution + context ~ variable, value.var = "value") |
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173 new_sig_matrix = format(new_sig_matrix, scientific=TRUE) |
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174 write.table(new_sig_matrix, file=opt$sigmatrix, quote = FALSE, row.names = FALSE, sep="\t") |
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175 grid.arrange(p5) |
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176 # Visualize the contribution of the signatures in a barplot |
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177 pc1 <- plot_contribution(nmf_res$contribution, nmf_res$signature, mode="relative", coord_flip = TRUE) |
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178 # Visualize the contribution of the signatures in absolute number of mutations |
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179 pc2 <- plot_contribution(nmf_res$contribution, nmf_res$signature, mode="absolute", coord_flip = TRUE) |
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180 # Combine the two plots: |
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181 grid.arrange(pc1, pc2) |
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182 |
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183 # The relative contribution of each signature for each sample can also be plotted as a heatmap with |
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184 # plot_contribution_heatmap, which might be easier to interpret and compare than stacked barplots. |
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185 # The samples can be hierarchically clustered based on their euclidean dis- tance. The signatures |
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186 # can be plotted in a user-specified order. |
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187 # Plot signature contribution as a heatmap with sample clustering dendrogram and a specified signature order: |
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188 pch1 <- plot_contribution_heatmap(nmf_res$contribution, |
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189 sig_order = paste0("NewSig_", 1:opt$newsignum)) |
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190 # Plot signature contribution as a heatmap without sample clustering: |
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191 pch2 <- plot_contribution_heatmap(nmf_res$contribution, cluster_samples=FALSE) |
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192 #Combine the plots into one figure: |
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193 grid.arrange(pch1, pch2, ncol = 2, widths = c(2,1.6)) |
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194 |
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195 # Compare the reconstructed mutational profile with the original mutational profile: |
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196 plot_compare_profiles(pseudo_mut_mat[,1], |
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197 nmf_res$reconstructed[,1], |
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198 profile_names = c("Original", "Reconstructed"), |
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199 condensed = TRUE) |
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200 dev.off() |
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201 } |
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202 ##### Section 3: Find optimal contribution of known signatures: COSMIC mutational signatures #### |
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203 |
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204 if (!is.na(opt$output_cosmic)[1]) { |
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205 pdf(opt$output_cosmic, paper = "special", width = 11.69, height = 11.69) |
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206 pseudo_mut_mat <- mut_mat + 0.0001 # First add a small psuedocount to the mutation count matrix |
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207 if (opt$cosmic_version == "v2") { |
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208 sp_url <- paste("https://cancer.sanger.ac.uk/cancergenome/assets/", "signatures_probabilities.txt", sep = "") |
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209 cancer_signatures = read.table(sp_url, sep = "\t", header = TRUE) |
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210 new_order = match(row.names(pseudo_mut_mat), cancer_signatures$Somatic.Mutation.Type) |
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211 cancer_signatures = cancer_signatures[as.vector(new_order),] |
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212 row.names(cancer_signatures) = cancer_signatures$Somatic.Mutation.Type |
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213 cancer_signatures = as.matrix(cancer_signatures[,4:33]) |
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214 colnames(cancer_signatures) <- gsub("Signature.", "", colnames(cancer_signatures)) # shorten signature labels |
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215 cosmic_tag <- "Signatures (Cosmic v2, March 2015)" |
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216 cosmic_colors <- col_vector[1:30] |
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217 } else { |
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218 sp_url <- "https://raw.githubusercontent.com/ARTbio/startbio/master/sigProfiler_SBS_signatures_2019_05_22.tsv" |
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219 cancer_signatures = read.table(sp_url, sep = "\t", header = TRUE) |
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220 new_order = match(row.names(pseudo_mut_mat), cancer_signatures$Somatic.Mutation.Type) |
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221 cancer_signatures = cancer_signatures[as.vector(new_order),] |
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222 row.names(cancer_signatures) = cancer_signatures$Somatic.Mutation.Type |
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223 cancer_signatures = as.matrix(cancer_signatures[,4:70]) |
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224 colnames(cancer_signatures) <- gsub("SBS", "", colnames(cancer_signatures)) # shorten signature labels |
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225 cosmic_tag <- "Signatures (Cosmic v3, May 2019)" |
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226 cosmic_colors <- col_vector[1:67] |
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227 } |
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228 |
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229 # Plot mutational profiles of the COSMIC signatures |
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230 if (opt$cosmic_version == "v2") { |
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231 p6 <- plot_96_profile(cancer_signatures, condensed = TRUE, ymax = 0.3) |
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232 grid.arrange(p6, top = textGrob("COSMIC signature profiles",gp=gpar(fontsize=12,font=3))) |
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233 } else { |
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234 p6 <- plot_96_profile(cancer_signatures[,1:33], condensed = TRUE, ymax = 0.3) |
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235 p6bis <- plot_96_profile(cancer_signatures[,34:67], condensed = TRUE, ymax = 0.3) |
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236 grid.arrange(p6, top = textGrob("COSMIC signature profiles (on two pages)",gp=gpar(fontsize=12,font=3))) |
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237 grid.arrange(p6bis, top = textGrob("COSMIC signature profiles (continued)",gp=gpar(fontsize=12,font=3))) |
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238 } |
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239 |
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240 |
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241 # Find optimal contribution of COSMIC signatures to reconstruct 96 mutational profiles |
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242 fit_res <- fit_to_signatures(pseudo_mut_mat, cancer_signatures) |
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243 |
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244 # Plot contribution barplots |
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245 pc3 <- plot_contribution(fit_res$contribution, cancer_signatures, coord_flip = T, mode = "absolute") |
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246 pc4 <- plot_contribution(fit_res$contribution, cancer_signatures, coord_flip = T, mode = "relative") |
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247 pc3_data <- pc3$data |
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248 pc3 <- ggplot(pc3_data, aes(x=Sample, y=Contribution, fill=as.factor(Signature))) + |
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249 geom_bar(stat="identity", position='stack') + |
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250 coord_flip() + |
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251 scale_fill_manual(name = "Cosmic\nSignatures", values = cosmic_colors) + |
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252 labs(x = "Samples", y = "Absolute contribution") + theme_bw() + |
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253 theme(panel.grid.minor.x = element_blank(), panel.grid.major.x = element_blank(), legend.position="right", |
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254 text = element_text(size=8), |
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255 axis.text.x = element_text(angle=90, hjust=1)) |
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256 pc4_data <- pc4$data |
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257 pc4 <- ggplot(pc4_data, aes(x=Sample, y=Contribution, fill=as.factor(Signature))) + |
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258 geom_bar(stat="identity", position='fill') + |
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259 coord_flip() + |
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260 scale_fill_manual(name = "Cosmic\nSignatures", values = cosmic_colors) + |
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261 scale_y_continuous(labels = scales::percent_format(accuracy = 1)) + |
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262 labs(x = "Samples", y = "Relative contribution") + theme_bw() + |
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263 theme(panel.grid.minor.x = element_blank(), panel.grid.major.x = element_blank(), legend.position="right", |
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264 text = element_text(size=8), |
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265 axis.text.x = element_text(angle=90, hjust=1)) |
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266 ##### |
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267 # ggplot2 alternative |
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268 if (!is.na(opt$levels)[1]) { # if there are levels to display in graphs |
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269 pc3_data <- pc3$data |
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270 pc3_data <- merge (pc3_data, metadata_table[,c(1,3)], by.x="Sample", by.y="element_identifier") |
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271 pc3 <- ggplot(pc3_data, aes(x=Sample, y=Contribution, fill=as.factor(Signature))) + |
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272 geom_bar(stat="identity", position='stack') + |
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273 scale_fill_manual(name = "Cosmic\nSignatures", values = cosmic_colors) + |
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274 labs(x = "Samples", y = "Absolute contribution") + theme_bw() + |
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275 theme(panel.grid.minor.x = element_blank(), panel.grid.major.x = element_blank(), legend.position="right", |
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276 text = element_text(size=8), |
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277 axis.text.x = element_text(angle=90, hjust=1)) + |
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278 facet_grid(~level, scales = "free_x", space="free") |
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279 pc4_data <- pc4$data |
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280 pc4_data <- merge (pc4_data, metadata_table[,c(1,3)], by.x="Sample", by.y="element_identifier") |
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281 pc4 <- ggplot(pc4_data, aes(x=Sample, y=Contribution, fill=as.factor(Signature))) + |
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282 geom_bar(stat="identity", position='fill') + |
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283 scale_fill_manual(name = "Cosmic\nSignatures", values = cosmic_colors) + |
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284 scale_y_continuous(labels = scales::percent_format(accuracy = 1)) + |
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285 labs(x = "Samples", y = "Relative contribution") + theme_bw() + |
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286 theme(panel.grid.minor.x = element_blank(), panel.grid.major.x = element_blank(), legend.position="right", |
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287 text = element_text(size=8), |
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288 axis.text.x = element_text(angle=90, hjust=1)) + |
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289 facet_grid(~level, scales = "free_x", space="free") |
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290 } |
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291 # Combine the two plots: |
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292 grid.arrange(pc3, pc4, top = textGrob("Absolute and Relative Contributions of Cosmic signatures to mutational patterns",gp=gpar(fontsize=12,font=3))) |
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293 |
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294 #### pie charts of comic signatures contributions in samples ### |
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295 library(reshape2) |
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296 library(dplyr) |
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297 if (length(levels(factor(levels_table$level))) < 2) { |
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298 fit_res_contrib <- as.data.frame(fit_res$contribution) |
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299 worklist <- cbind(signature=rownames(fit_res$contribution), |
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300 level=rep("nolabels", length(fit_res_contrib[,1])), |
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301 fit_res_contrib, |
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302 sum=rowSums(fit_res_contrib)) |
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303 worklist <- worklist[order(worklist[ ,"sum"], decreasing = T), ] |
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304 worklist <- worklist[1:opt$signum,] |
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305 worklist <- worklist[ , -length(worklist[1,])] |
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306 worklist <- melt(worklist) |
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307 worklist <- worklist[,c(1,3,4,2)] |
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308 } else { |
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309 worklist <- list() |
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310 for (i in levels(factor(levels_table$level))) { |
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311 fit_res$contribution[,levels_table$element_identifier[levels_table$level == i]] -> worklist[[i]] |
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312 sum <- rowSums(as.data.frame(worklist[[i]])) |
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313 worklist[[i]] <- cbind(worklist[[i]], sum) |
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314 worklist[[i]] <- worklist[[i]][order(worklist[[i]][ ,"sum"], decreasing = T), ] |
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315 worklist[[i]] <- worklist[[i]][1:opt$signum,] |
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316 worklist[[i]] <- worklist[[i]][ , -length(as.data.frame(worklist[[i]]))] |
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317 } |
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318 worklist <- as.data.frame(melt(worklist)) |
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319 } |
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320 |
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321 colnames(worklist) <- c("signature", "sample", "value", "level") |
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322 print(worklist) |
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323 worklist <- as.data.frame(worklist %>% group_by(sample) %>% mutate(value=value/sum(value)*100)) |
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324 worklist$pos <- cumsum(worklist$value) - worklist$value/2 |
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325 worklist$label <- factor(worklist$signature) |
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326 worklist$signature <- factor(worklist$signature) |
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327 p7 <- ggplot(worklist, aes(x="", y=value, group=signature, fill=signature)) + |
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328 geom_bar(width = 1, stat = "identity") + |
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329 geom_text(aes(label = label), position = position_stack(vjust = 0.5), color="black", size=3) + |
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330 coord_polar("y", start=0) + facet_wrap(~ sample) + |
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331 labs(x="", y="Samples", fill = cosmic_tag) + |
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332 scale_fill_manual(name = paste0(opt$signum, " most contributing\nsignatures\n(in each label/tissue)"), |
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333 values = cosmic_colors[as.numeric(levels(factor(worklist$label)))]) + |
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334 theme(axis.text = element_blank(), |
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335 axis.ticks = element_blank(), |
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336 panel.grid = element_blank()) |
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337 grid.arrange(p7) |
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338 |
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339 # Plot relative contribution of the cancer signatures in each sample as a heatmap with sample clustering |
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340 if (length(vcf_paths) > 1) { |
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341 p8 <- plot_contribution_heatmap(fit_res$contribution, cluster_samples = TRUE, method = "complete") |
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342 grid.arrange(p8) |
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343 } |
2
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344 |
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345 # calculate all pairwise cosine similarities |
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346 cos_sim_ori_rec <- cos_sim_matrix(pseudo_mut_mat, fit_res$reconstructed) |
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347 # extract cosine similarities per sample between original and reconstructed |
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348 cos_sim_ori_rec <- as.data.frame(diag(cos_sim_ori_rec)) |
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349 |
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350 # We can use ggplot to make a barplot of the cosine similarities between the original and |
aea952be68cb
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mutational_patterns commit cd8f633245d53cf47eaf860a4e0ae8d806c34419"
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351 # reconstructed mutational profile of each sample. This clearly shows how well each mutational |
aea952be68cb
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352 # profile can be reconstructed with the COSMIC mutational signatures. Two identical profiles |
aea952be68cb
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353 # have a cosine similarity of 1. The lower the cosine similarity between original and |
aea952be68cb
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354 # reconstructed, the less well the original mutational profile can be reconstructed with |
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355 # the COSMIC signatures. You could use, for example, cosine similarity of 0.95 as a cutoff. |
aea952be68cb
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356 |
aea952be68cb
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357 # Adjust data frame for plotting with gpplot |
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358 colnames(cos_sim_ori_rec) = "cos_sim" |
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359 cos_sim_ori_rec$sample = row.names(cos_sim_ori_rec) |
aea952be68cb
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360 # Make barplot |
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361 p9 <- ggplot(cos_sim_ori_rec, aes(y=cos_sim, x=sample)) + |
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362 geom_bar(stat="identity", fill = "skyblue4") + |
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363 coord_cartesian(ylim=c(0.8, 1)) + |
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364 # coord_flip(ylim=c(0.8,1)) + |
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365 ylab("Cosine similarity\n original VS reconstructed") + |
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366 xlab("") + |
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367 # Reverse order of the samples such that first is up |
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368 # xlim(rev(levels(factor(cos_sim_ori_rec$sample)))) + |
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369 theme_bw() + |
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370 theme(panel.grid.minor.y=element_blank(), |
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371 panel.grid.major.y=element_blank()) + |
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372 # Add cut.off line |
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373 geom_hline(aes(yintercept=.95)) |
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374 grid.arrange(p9, top = textGrob("Similarity between true and reconstructed profiles (with all Cosmic sig.)",gp=gpar(fontsize=12,font=3))) |
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375 dev.off() |
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376 } |
3
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377 |
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378 |
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379 # Output RData file |
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380 if (!is.null(opt$rdata)) { |
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381 save.image(file=opt$rdata) |
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382 } |