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diff rsem-bwt2.xml @ 5:835a2f3372b5 draft

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planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit 5bb908607e13ccad44d768e786253ce7b5c626f8
author artbio
date Sat, 02 Jun 2018 06:10:54 -0400
parents 1823c8e07d96
children 45a30e216fec
line wrap: on
line diff
--- a/rsem-bwt2.xml	Fri Jun 01 03:00:34 2018 -0400
+++ b/rsem-bwt2.xml	Sat Jun 02 06:10:54 2018 -0400
@@ -163,7 +163,7 @@
       </param>
       <when value="index">
         <conditional name="self_reference">
-          <param name="ref_type" type="select" label="Reference transcript source">
+          <param name="ref_type" type="select" label="Reference source">
             <option value="transcripts">transcript fasta</option>
             <option value="genomic">reference genome and gtf</option>
           </param>
@@ -228,7 +228,7 @@
         <conditional name="reference">
           <param name="refSrc" type="select" label="RSEM Reference Source">
             <option value="history">From your history</option>
-            <option value="self">Prepare RSEM Reference with this tool</option>
+            <option value="self">RSEM Reference prepared with this tool</option>
           </param>
           <when value="history">
             <param name="rsem_ref" type="data" format="rsem_bt2_ref" label="RSEM reference" />
@@ -326,50 +326,108 @@
    <test>
       <param name="select_job" value="index"/>
       <param name="ref_type" value="genomic"/>
-      <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
-      <param name="gtf" value="ref.gtf" ftype="gtf"/>
-      <param name="reference_name" value="ref"/>
+      <param name="reference_fasta_file" value="eboVir3.fa" ftype="fasta"/>
+      <param name="gtf" value="eboVir3.gtf" ftype="gtf"/>
+      <param name="reference_name" value="ebola_genome"/>
       <param name="select" value="Yes"/>
-      <param name="sample" value="rsem_sample"/>
+      <param name="sample" value="ebola_RNAseq"/>
       <param name="refSrc" value="self"/>
       <param name="format" value="fastq"/>
       <param name="matepair" value="single"/>
-      <param name="singlefastq" value="test.fq" ftype="fastqsanger"/>
+      <param name="singlefastq" value="Ebola_forward.fastqsanger" ftype="fastqsanger"/>
       <param name="result_bams" value="none"/>
       <output name="reference_file">
         <assert_contents>
-          <has_text text="ref" />
+          <has_text text="ebola_genome" />
         </assert_contents>
       </output>
-      <output name="gene_abundances" value="gene_abundances.tab2"/> 
-      <output name="isoform_abundances" value="isoform_abundances.tab2" /> 
+      <output name="gene_abundances" value="refgen_gene_abundances.bt2.tab"/> 
+      <output name="isoform_abundances" value="refgen_isoform_abundances.bt2.tab" /> 
       <output name="log"> 
         <assert_contents>
           <has_text text="Expression Results are written" />
         </assert_contents>
       </output>
     </test>
-      <!-- ungzipped paired-end fastqsanger -->
     <test>
+      <!-- test paired end on ebola genome -->
       <param name="select_job" value="index"/>
       <param name="ref_type" value="genomic"/>
-      <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
-      <param name="gtf" value="ref.gtf" ftype="gtf"/>
-      <param name="reference_name" value="ref"/>
+      <param name="reference_fasta_file" value="eboVir3.fa" ftype="fasta"/>
+      <param name="gtf" value="eboVir3.gtf" ftype="gtf"/>
+      <param name="reference_name" value="ebola"/>
+      <param name="select" value="Yes"/>
+      <param name="sample" value="ebola_RNAseq"/>
+      <param name="refSrc" value="self"/>
+      <param name="format" value="fastq"/>
+      <param name="matepair" value="paired"/>
+      <param name="fastq1" value="Ebola_forward.fastqsanger.gz" ftype="fastqsanger.gz"/>
+      <param name="fastq2" value="Ebola_reverse.fastqsanger.gz" ftype="fastqsanger.gz"/>
+      <param name="result_bams" value="both"/>
+      <output name="reference_file">
+        <assert_contents>
+          <has_text text="ebola" />
+        </assert_contents>
+      </output>
+      <output name="gene_abundances" value="paired_refgen_gene_abundances.bt2.tab"/>
+      <output name="isoform_abundances" value="paired_refgen_isoform_abundances.bt2.tab" />
+      <output name="transcript_sorted_bam" value="paired_refgen_transcripts.bt2.bam" ftype="bam" lines_diff="2" />
+      <output name="genome_sorted_bam" value="paired_refgen_isoform_abundances.bt2.bam" ftype="bam" lines_diff="2" />
+      <output name="log">
+        <assert_contents>
+          <has_text text="Expression Results are written" />
+        </assert_contents>
+      </output>
+    </test>
+     <test>
+      <!-- test paired end on ebola transcripts -->
+      <param name="select_job" value="index"/>
+      <param name="ref_type" value="transcripts"/>
+      <param name="reference_fasta_file" value="Ebola_transcripts.fa" ftype="fasta"/>
+      <param name="transcript_to_gene_map" value="ebola_gene_transcript_table.txt" ftype="gtf"/>
+      <param name="reference_name" value="ebola"/>
+      <param name="select" value="Yes"/>
+      <param name="sample" value="ebola_RNAseq"/>
+      <param name="refSrc" value="self"/>
+      <param name="format" value="fastq"/>
+      <param name="matepair" value="paired"/>
+      <param name="fastq1" value="Ebola_forward.fastqsanger.gz" ftype="fastqsanger.gz"/>
+      <param name="fastq2" value="Ebola_reverse.fastqsanger.gz" ftype="fastqsanger.gz"/>
+      <param name="result_bams" value="default"/>
+      <output name="reference_file">
+        <assert_contents>
+          <has_text text="ebola" />
+        </assert_contents>
+      </output>
+      <output name="gene_abundances" value="paired_reftrans_gene_abundances.bt2.tab"/>
+      <output name="isoform_abundances" value="paired_reftrans_isoform_abundances.bt2.tab" />
+      <output name="transcript_sorted_bam" value="paired_reftrans_transcripts.bt2.bam" ftype="bam" lines_diff="2" />
+      <output name="log">
+        <assert_contents>
+          <has_text text="Expression Results are written" />
+        </assert_contents>
+      </output>
+    </test>
+   <test>
+      <param name="select_job" value="index"/>
+      <param name="ref_type" value="genomic"/>
+      <param name="reference_fasta_file" value="eboVir3.fa" ftype="fasta"/>
+      <param name="gtf" value="eboVir3.gtf" ftype="gtf"/>
+      <param name="reference_name" value="ebola"/>
       <param name="select" value="Yes"/>
       <param name="sample" value="rsem_sample"/>
       <param name="refSrc" value="self"/>
       <param name="format" value="fastq"/>
       <param name="matepair" value="single"/>
-      <param name="singlefastq" value="test.fastq.gz" ftype="fastqsanger.gz"/>
+      <param name="singlefastq" value="Ebola_reverse.fastqsanger.gz" ftype="fastqsanger.gz"/>
       <param name="result_bams" value="none"/>
       <output name="reference_file">
         <assert_contents>
-          <has_text text="ref" />
+          <has_text text="ebola" />
         </assert_contents>
       </output>
-      <output name="gene_abundances" value="gene_abundances.tab2"/>
-      <output name="isoform_abundances" value="isoform_abundances.tab2" />
+      <output name="gene_abundances" value="single_refgen_gene_abundances.bt2.tab"/>
+      <output name="isoform_abundances" value="single_refgen_isoform_abundances.bt2.tab" />
       <output name="log">
         <assert_contents>
           <has_text text="Expression Results are written" />
@@ -379,13 +437,13 @@
     <test>
       <param name="select_job" value="index"/>
       <param name="ref_type" value="genomic"/>
-      <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
-      <param name="gtf" value="ref.gtf" ftype="gtf"/>
-      <param name="reference_name" value="ref"/>
+      <param name="reference_fasta_file" value="eboVir3.fa" ftype="fasta"/>
+      <param name="gtf" value="eboVir3.gtf" ftype="gtf"/>
+      <param name="reference_name" value="alone"/>
       <param name="select" value="No"/>
       <output name="reference_file">
         <assert_contents>
-          <has_text text="ref" />
+          <has_text text="alone" />
         </assert_contents>
       </output>
     </test>