Mercurial > repos > artbio > sequence_format_converter

annotate sequence_format_converter.py @ 0:a8aacccd79a3 draft

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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
author artbio
date Mon, 04 Sep 2017 07:13:28 -0400
parents
children 9ce7ccd468aa
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0
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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diff changeset
1 #!/usr/bin/env python
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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2 #
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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3 import argparse
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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4 import logging
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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5 import sys
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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6 from collections import defaultdict
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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7
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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8
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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9 def Parser():
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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10 the_parser = argparse.ArgumentParser()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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11 the_parser.add_argument(
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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12 '--input', action="store", type=str,
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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13 help="input file, accepted format: fastq, fasta, fasta_weigthed, \
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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14 tabular")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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15 the_parser.add_argument(
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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16 '--output', action="store", type=str, help="output converted file")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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17 the_parser.add_argument(
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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18 '--format', action="store", type=str,
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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19 help="select output format (fasta, fasta_weigthed, tabular")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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20 args = the_parser.parse_args()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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21 return args
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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22
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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23
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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24 class Sequencing:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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25
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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26 def __init__(self, input, output, format):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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27 self.input = input
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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28 self.output = open(output, 'w')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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29 self.outputformat = format
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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30 self.inputformat = self.detectformat(self.input)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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31 self.seqdic = defaultdict(int)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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32 self.read(self.input, self.inputformat)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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33 self.write(self.output, self.outputformat)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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34
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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35 def detectformat(self, input):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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36 input = open(input, 'r')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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37 block = []
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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38 reference = ['A', 'T', 'G', 'C', 'N']
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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39 format = ''
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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40 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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41 for l in range(4):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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42 block.append(input.readline()[:-1])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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43 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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44 logging.info("File hasn't at leat four lines !")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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45 sys.exit("File hasn't at leat four lines !")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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46 input.close()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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47 line1, line2, line3, line4 = block[0], block[1], block[2], block[3]
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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48 if line1[0] == '>' and line3[0] == '>':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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49 logging.info("'>' detected in lines 1 and 3")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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50 sequence = ''.join([line2, line4]).upper()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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51 nucleotides = set([base for base in sequence])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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52 for nucleotide in nucleotides:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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53 if nucleotide not in reference:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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54 logging.info("But other nucleotides that A, T, G, C or N")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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55 sys.exit('input appears to be Fasta but with \
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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56 unexpected nucleotides')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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57 format = 'fasta'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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58 elif line1[0] == '>' and line4[0] == '>':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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59 logging.info("'>' detected in lines 1 and 4")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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60 sequence = ''.join([line2, line3]).upper()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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61 nucleotides = set([base for base in sequence])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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62 for nucleotide in nucleotides:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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63 if nucleotide not in reference:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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64 logging.info("But other nucleotides that A, T, G, C or N")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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65 sys.exit('input appears to be Fasta but with \
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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66 unexpected nucleotides')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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67 format = 'fasta'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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68 elif line1[0] == '>':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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69 logging.info("'>' detected in lines 1")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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70 sequence = ''.join([line2, line3, line4]).upper()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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71 nucleotides = set([base for base in sequence])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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72 for nucleotide in nucleotides:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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73 if nucleotide not in reference:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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74 logging.info("But other nucleotides that A, T, G, C or N")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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75 sys.exit('input appears to be Fasta but with \
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
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76 unexpected nucleotides')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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77 format = 'fasta'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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78 if format == 'fasta':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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79 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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80 for line in block:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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81 if line[0] == '>':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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82 int(line.split('_')[-1])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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83 return 'fastaw'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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84 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
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85 return 'fasta'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
86 if line1[0] == '@' and line3[0] == '+':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
87 nucleotides = set([base for base in line2])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
88 for nucleotide in nucleotides:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
89 if nucleotide not in reference:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
90 logging.info("Looks like fastq input but other nucleotides \
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
91 that A, T, G, C or N")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
92 sys.exit("input appears to be Fastq \
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
93 but with unexpected nucleotides")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
94 return 'fastq'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
95 for line in block:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
96 if len(line.split('\t')) != 2:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
97 logging.info("No valid format detected")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
98 sys.exit('No valid format detected')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
99 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
100 int(line.split('\t')[-1])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
101 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
102 logging.info("No valid format detected")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
103 sys.exit('No valid format detected')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
104 for nucleotide in line.split('\t')[0]:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
105 if nucleotide not in reference:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
106 logging.info("No valid format detected")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
107 sys.exit('No valid format detected')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
108 return 'tabular'
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
109
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
110 def read(self, input, format):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
111 input = open(input, 'r')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
112 if format == 'fasta':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
113 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
114 self.readfasta(input)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
115 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
116 logging.info("an error occured while reading fasta")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
117 elif format == 'fastaw':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
118 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
119 self.readfastaw(input)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
120 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
121 logging.info("an error occured while reading fastaw")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
122 elif format == 'tabular':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
123 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
124 self.readtabular(input)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
125 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
126 logging.info("an error occured while reading tabular")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
127 elif format == 'fastq':
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
128 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
129 self.readfastq(input)
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
130 except:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
131 logging.info("an error occured while reading fastq")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
132 else:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
133 logging.info("no valid format detected")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
134 sys.exit('No valid format detected')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
135
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
136 def readfastaw(self, input):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
137 for line in input:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
138 if line[0] == ">":
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
139 weigth = int(line[:-1].split("_")[-1])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
140 else:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
141 self.seqdic[line[:-1]] += weigth
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
142 input.close()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
143
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
144 def readfasta(self, input):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
145 ''' this method is able to read multi-line fasta sequence'''
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
146 for line in input:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
147 if line[0] == ">":
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
148 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
149 # to dump the sequence of the previous item
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
150 # try because of first missing stringlist variable
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
151 self.seqdic["".join(stringlist)] += 1
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
152 except NameError:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
153 pass
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
154 stringlist = []
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
155 else:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
156 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
157 stringlist.append(line[:-1])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
158 except UnboundLocalError:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
159 # if file went through filter and contains only empty lines
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
160 logging.info("first line is empty.")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
161 try:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
162 self.seqdic["".join(stringlist)] += 1 # for the last sequence
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
163 except NameError:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
164 logging.info("input file has not fasta sequences.")
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
165 input.close()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
166
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
167 def readtabular(self, input):
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
168 for line in input:
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
169 fields = line[:-1].split('\t')
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
170 self.seqdic[fields[0]] += int(fields[1])
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
171 input.close()
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
diff changeset
172
a8aacccd79a3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sequence_format_converter commit d6ef80f9db43eae4f58b33f58b5ef6f8209907db
artbio
parents:
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173 def readfastq(self, input):
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174 linecount = 0
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175 for line in input:
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176 linecount += 1
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177 if linecount % 4 == 2:
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178 self.seqdic[line[:-1]] += 1
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179 input.close()
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180
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181 def write(self, output, format='fasta'):
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182 if format == 'fasta':
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183 headercount = 0
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184 for seq in sorted(self.seqdic, key=self.seqdic.get, reverse=True):
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185 for i in range(self.seqdic[seq]):
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186 headercount += 1
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187 output.write('>%s\n%s\n' % (headercount, seq))
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188 elif format == 'fastaw':
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189 headercount = 0
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190 for seq in sorted(self.seqdic, key=self.seqdic.get, reverse=True):
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191 headercount += 1
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192 output.write('>%s_%s\n%s\n' % (headercount,
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193 self.seqdic[seq], seq))
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194 elif format == 'tabular':
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195 for seq in sorted(self.seqdic, key=self.seqdic.get, reverse=True):
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196 output.write('%s\t%s\n' % (seq, self.seqdic[seq]))
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197 output.close()
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198
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199
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200 def main(input, output, format):
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201 Sequencing(input, output, format)
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202
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203
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204 if __name__ == "__main__":
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205 args = Parser()
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206 log = logging.getLogger(__name__)
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207 logging.basicConfig(stream=sys.stdout, level=logging.INFO)
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208 main(args.input, args.output, args.format)