<tool id="artbio_size_histogram" name="Generate read size histograms" version="1.0.0">
    <description>from alignment files</description>
    <requirements>
        <requirement type="package" version="1.2.0=py27_0">bowtie</requirement>
        <requirement type="package" version="0.11.2.1=py27_0">pysam</requirement>
        <requirement type="package" version="1.9.3">numpy</requirement>
        <requirement type="package" version="1.3.2=r3.3.2_0">r-optparse</requirement>
        <requirement type="package" version="0.6_28=r3.3.2_0">r-latticeextra</requirement>
        <requirement type="package" version="2.2.1=r3.3.2_0">r-gridextra</requirement>
    </requirements>
    <command detect_errors="exit_code"><![CDATA[
        python '$__tool_directory__'/size_histogram.py
        #if $refGenomeSource.genomeSource == "history":
            --reference_fasta  ## sys.argv[2]
            '$refGenomeSource.ownFile' ## index source
        #else:
            #silent reference= filter( lambda x: str( x[0] ) == str( $refGenomeSource.series[0].input.dbkey ), $__app__.tool_data_tables[ 'bowtie_indexes' ].get_fields() )[0][-1]
            --reference_bowtie_index
            '$reference'
        #end if
        --output_size_distribution
        '$size_distribution_dataframe'
        --minquery
        $minquery
        --maxquery
        $maxquery
        --input
        #for $i in $refGenomeSource.series
            '$i.input'
        #end for
        --ext
        #for $i in $refGenomeSource.series
            '$i.input.ext'
        #end for
        --label
        #for $i in $refGenomeSource.series
            "$i.input.element_identifier"
        #end for
        #if $gff:
            --gff '$gff'
        #end if
        #if $global.value == 'yes':
            --global_size
        #end if
        #if $collapsestrands.value == 'yes':
            --collapse
        #end if
        --normalization_factor
        #for $i in $refGenomeSource.series
            $i.norm
        #end for
        &&
        Rscript '$__tool_directory__'/size_histogram.r
            --global '$global'
            --size_distribution_tab '$size_distribution_dataframe'
            --size_distribution_pdf '$size_PDF'
            --title '$title'
            --ylabel '$ylabel'
            --yrange '$yrange'
            --rows_per_page '$rows_per_page'
    ]]></command>
    <inputs>
        <conditional name="refGenomeSource">
            <param name="genomeSource" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options">
                <option value="indexed">Use a built-in index</option>
                <option value="history">Use one from the history</option>
            </param>
            <when value="indexed">
                <repeat name="series" title="Add alignment files">
                    <param name="input" type="data" label="Select multiple alignments to parse" format="tabular,sam,bam">
                        <validator type="dataset_metadata_in_data_table" table_name="bowtie_indexes" metadata_name="dbkey" metadata_column="0" message="database not set for this bowtie output. Select the database(=genome used for matching) manually, or select a reference fasta from your history."/>
                    </param>
                    <param name="norm" type="float" value="1" label="Indicate a normalization factor to compare multiple aligments"/>
                </repeat>
            </when>
            <when value="history">
                <param name="ownFile" type="data" format="fasta" label="Select a fasta file, to serve as index reference" />
                <repeat name="series" title="Add alignment files">
                    <param name="input" type="data" label="Select multiple alignments to parse" format="tabular,sam,bam"/>
                    <param name="norm" type="float" value="1" label="Indicate a normalization factor to compare multiple aligments"/>
                </repeat>
            </when>
        </conditional>
        <param name="gff" type="data" format="gff,gff3" optional="true" label="Optional: select a GFF to investigate regions of interest" help="GFF must match genome build"/>
        <!-- <validator type="dataset_metadata_in_data_table" table_name="bowtie_indexes" metadata_name="dbkey" metadata_column="0" message="GFF database and alignment file databse do not match!"/> -->
        <param name="global" type="select" label="Generate size distribution for each item, or generate a global alignment">
            <option value="no">for each item</option>
            <option value="yes">global</option>
        </param>
        <param name="collapsestrands" type="select" label="Whether + and - reads should be collapsed or not">
            <option value="no">Do not collapse</option>
            <option value="yes">Collapse + and - reads</option>
        </param>
        <param name="minquery" type="integer" size="3" value="18" label="Min size of reads to plot" help="'15' = 15 nucleotides"/>
        <param name="maxquery" type="integer" size="3" value="28" label="Max size of reads to plot" help="'30' = 30 nucleotides"/>
        <param name="title" type="text" size="15" value="Size distribution" label="Main Titles"/>
        <param name="xlabel" type="text" size="15" value="Size in nucleotides" label="x axis label"/>
        <param name="ylabel" type="text" size="15" value="Number of reads" label="y axis label"/>
        <param name="yrange" type="integer" size="3" value="0" label="y axis range for size distributions. 0 means auto-scaling."/>
        <param name="rows_per_page" type="text" size="9" value="8" label="How many items to display per page?">
            <validator type="in_range" min="6" max="20" message="Select between 6 and 20 rows, as the readability will suffer otherwise."/>
        </param>
    </inputs>

    <outputs>
        <data format="tabular" name="size_distribution_dataframe" label="Size_distribution_dataframe.tab"/>
        <data format="pdf" name="size_PDF" label="Size_distribution.pdf"/>
    </outputs>

<help>

**What it does**

Takes one or more alignment files (BAM, SAM or tabular bowtie output) as input and produces a histogram of read sizes,
where by default for each "chromosome" a histogram of read sizes is drawn.
Reads that map in sense are on the top (red), reads that map antisense are on the bottom (blue).


.. class:: warningmark

'''TIP''' The input data can be produced using the sRbowtie tool.

----

'''Example'''

Query sequence::
For a SAM file as the following:

  5	16	2L_79	24393	255	17M	*	0	0	CCTTCATCTTTTTTTTT	IIIIIIIIIIIIIIIII	XA:i:0	MD:Z:17	NM:i:0

  11	0	2R_1	12675	255	21M	*	0	0	AAAAAAAACGCGTCCTTGTGC	IIIIIIIIIIIIIIIIIIIII	XA:i:0	MD:Z:21	NM:i:0

  2	16	2L_5	669	255	23M	*	0	0	TGTTGCTGCATTTCTTTTTTTTT	IIIIIIIIIIIIIIIIIIIIIII	XA:i:0	MD:Z:23	NM:i:0

produce a plot like this:

----

.. image:: static/images/size_histogram.png
    :height: 800
    :width: 500

</help>
    <tests>
        <test>
            <param name="genomeSource" value="history" />
            <param name="ownFile" value="transposons.fasta" ftype="fasta" />
            <param name="series_0|input" value="sample1.srbowtie_out" ftype="tabular"/>
            <param name="series_0|norm" value="1" />
            <param name="series_1|input" value="sample2.srbowtie_out" ftype="tabular"/>
            <param name="series_1|norm" value="1" />
            <param name="series_2|input" value="sample3.srbowtie_out" ftype="tabular"/>
            <param name="series_2|norm" value="1" />
            <param name="global" value="no" />
            <param name="collapsestrands" value="no" />
            <param name="minquery" value="18"/>
            <param name="maxquery" value="30"/>
            <param name="title" value="Size distribution"/>
            <param name="xlabel" value="Size in nucleotides"/>
            <param name="ylabel" value="Number of reads"/>
            <param name="rows_per_page" value="10"/>
            <output name="size_distribution_dataframe" ftype="tabular" file="Size_distribution_dataframe.tab" />
            <output name="size_PDF" ftype="pdf" file="Size_distribution.pdf" />
        </test>
    </tests>
</tool>