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annotate small_rna_maps.xml @ 20:de7fbcb1348c draft

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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
author artbio
date Sun, 25 Nov 2018 06:56:40 -0500
parents f33afecac67a
children b585cb347a26
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20
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1 <tool id="small_rna_maps" name="small_rna_maps" version="2.10.0">
0
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2 <description></description>
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3 <requirements>
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4 <requirement type="package" version="1.11.2=py27_0">numpy</requirement>
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5 <requirement type="package" version="0.11.2.1=py27_0">pysam</requirement>
5
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6 <requirement type="package" version="1.3.2=r3.3.2_0">r-optparse</requirement>
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7 <requirement type="package" version="0.6_28=r3.3.2_0">r-latticeextra</requirement>
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8 <requirement type="package" version="2.2.1=r3.3.2_0">r-gridextra</requirement>
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9 <requirement type="package" version="1.4.2=r3.3.2_0">r-reshape2</requirement>
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10 <requirement type="package" version="0.6.6">sambamba</requirement>
0
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11 </requirements>
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12 <stdio>
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13 <exit_code range="1:" level="fatal" description="Tool exception" />
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14 </stdio>
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15 <command detect_errors="exit_code"><![CDATA[
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16 #for $file in $series
16
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17 sambamba view -t \${GALAXY_SLOTS} -F "not unmapped and sequence_length >= ${minsize} and sequence_length <= ${maxsize}" -f bam '$file.inputs' >'$file.inputs.name' &&
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18 samtools index '$file.inputs.name' &&
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19 #end for
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20 python '$__tool_directory__'/small_rna_maps.py
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21 --inputs
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22 #for $file in $series
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23 '$file.inputs.name'
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24 #end for
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25 --sample_names
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26 #for $sample in $series
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27 '$sample.inputs.name'
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28 #end for
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29 --minsize $minsize
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30 --maxsize $maxsize
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31 #if str($plots_options.plots_options_selector ) == "two_plot":
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32 --plot_methods '${plots_options.first_plot}' '${plots_options.extra_plot}'
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33 --outputs '$output_tab' '$extra_output_tab' &&
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34 #elif str($plots_options.plots_options_selector ) == "global":
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35 --plot_methods 'Size'
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36 --outputs '$output_tab' &&
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37 #elif str($plots_options.plots_options_selector ) == "cluster":
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38 --plot_methods 'Counts'
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39 --outputs '$output_tab'
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40 --cluster ${plots_options.cluster}
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41 --bed '$output_bed'
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42 --bed_skipsize ${plots_options.skip_size}
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43 --bed_skipcounts ${plots_options.skip_counts}
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44 --bed_skipdensity ${plots_options.skip_density}
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45 ${plots_options.strandness} &&
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46 #else:
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47 --plot_methods '${plots_options.first_plot}'
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48 --outputs '$output_tab' &&
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49 #end if
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50
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51
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52 Rscript '$__tool_directory__'/small_rna_maps.r
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53 --first_dataframe '$output_tab'
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54 --extra_dataframe '$extra_output_tab'
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55 --normalization
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56 #set $norm = ""
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57 #for $file in $series
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58 #set $norm += str($file.normalization)+' '
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59 #end for
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60 #set $norm = $norm[:-1]
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61 '$norm'
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62 #if $ylimits_cond.ylimits == "no":
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63 --ymin '' --ymax ''
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64 #else:
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65 --ymin '${ylimits_cond.ymin}' --ymax '${ylimits_cond.ymax}'
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66 #end if
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67 #if str($plots_options.plots_options_selector ) == "two_plot":
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68 --first_plot_method '${plots_options.first_plot}'
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69 --extra_plot_method '${plots_options.extra_plot}'
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70 #elif str($plots_options.plots_options_selector ) == "global":
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71 --first_plot_method 'Size'
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72 --extra_plot_method ''
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73 --global '${plots_options.mergestrands}'
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74 #else:
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75 --first_plot_method '${plots_options.first_plot}'
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76 --extra_plot_method ''
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77 #end if
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78 --output_pdf '$output_pdf'
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79 ]]></command>
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80 <inputs>
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81 <repeat name="series" title="Input" min="1">
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82 <param name="inputs" type="data" format="bam" label="Select a single alignment file to parse" multiple="false"
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83 help="If 'multiple datasets' method is used, the same normalization factor will be applied to each dataset. Please refer to the help section for more information." />
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84 <param name="normalization" type="float" label="Enter a size/normalization factor."
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85 help="Enter only one value e.g. '0.75' or '1.23'" value="1.0"/>
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86 </repeat>
8
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87 <param name="minsize" type="integer" label="Minimal size of reads for inclusion in analysis"
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88 value="0" help="default value: 0" />
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89 <param name="maxsize" type="integer" label="Maximal size of reads for inclusion in analysis"
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90 value="10000" help="default value: 10000" />
5
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91 <conditional name="plots_options">
7
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92 <param name="plots_options_selector" type="select" display="radio" label="Plot Options">
5
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93 <option value="one_plot">Just one plot per chromosome</option>
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94 <option value="two_plot" selected="True">Two plots per chromosome</option>
15
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95 <option value="global">Global read size distributions of aligned reads</option>
16
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96 <option value="cluster">Map read clusters</option>
5
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97 </param>
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98 <when value="two_plot">
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99 <param name="first_plot" type="select" display="radio" label="Select the type of the top plot">
15
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100 <option value="Counts">Counts</option>
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101 <option value="Coverage">Coverage</option>
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102 <option value="Mean">Mean Sizes</option>
5
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103 <option value="Median">Median Sizes</option>
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104 <option value="Size">Size Distributions</option>
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105 </param>
12
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106 <param name="extra_plot" type="select" display="radio" label="Select the type of the bottom plot">
15
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107 <option value="Counts">Counts</option>
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108 <option value="Coverage">Coverage</option>
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109 <option value="Mean">Mean Sizes</option>
5
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110 <option value="Median">Median Sizes</option>
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111 <option value="Size">Size Distributions</option>
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112 </param>
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113 </when>
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114 <when value="one_plot">
12
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115 <param name="first_plot" type="select" display="radio" label="select the type of plot">
15
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116 <option value="Counts">Counts</option>
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117 <option value="Coverage">Coverage</option>
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118 <option value="Mean">Mean Sizes</option>
5
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119 <option value="Median">Median Sizes</option>
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120 <option value="Size">Size Distributions</option>
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121 </param>
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122 </when>
7
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123 <when value="global">
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124 <param name="first_plot" type="hidden" value="Size"/>
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125 <param name="mergestrands" type="select" display="radio" label="Whether forward and reverse aligned reads should be merged or not in the histogram">
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126 <option value="nomerge">Do not merge</option>
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127 <option value="merge">Merge forward and reverse reads</option>
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128 </param>
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129 </when>
16
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130 <when value="cluster">
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131 <param name="first_plot" type="hidden" value="Counts"/>
20
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132 <param name="cluster" type="integer" label="Clustering distance in nucleotides" value="1"
16
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133 help="Sets the distance (in nt) below which reads are clustered to a single median position" />
17
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134 <param name="strandness" argument="--nostrand" type="boolean" truevalue="--nostrand" falsevalue="" checked="false"
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135 label="Ignore polarity of reads ?" help="Set if you wish to cluster reads regardless of whether they are forward or reverse"/>
20
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136 <param name="skip_size" type="integer" label="do not report clusters whose size is less than the specified value" value="1"
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137 help="Cluster size threshod (in nucleotides) for reporting. Set to 1 (default) reports all clusters, including singlets" />
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138 <param name="skip_counts" type="integer" label="do not report cluster with a number of reads lower than the specified value" value="1"
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139 help="Number-of-reads threshod (in nucleotides) for cluster reporting. Set to 1 (default) reports all clusters, irrespective of their counts" />
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140 <param name="skip_density" type="float" label="do not report cluster with density equal or less than the specified value" value="0"
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141 help="Density threshod (in reads per nucleotides) for reporting. Set to 0 (default) reports all cluster densities" />
16
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142 </when>
5
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143 </conditional>
12
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144 <conditional name="ylimits_cond">
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145 <param name="ylimits" type="boolean" truevalue="yes" falsevalue="no" checked="false" label="Do you wish to set an y axis limit to the plots?"
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146 help="This limit won't be applied to size distribution plots"/>
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147 <when value="yes">
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148 <param name="ymin" type="float" label="Enter minimum value" value="0.0" help="e.g. '-5.0'"/>
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149 <param name="ymax" type="float" label="Enter maximum value" value="0.0" help="e.g. '5.0'"/>
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150 </when>
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151 <when value="no">
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152 </when>
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153 </conditional>
0
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154 </inputs>
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155
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156 <outputs>
7
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157 <data format="tabular" name="output_tab" label="$plots_options.first_plot dataframe" />
18
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158 <data format="bed" name="output_bed" label="bed file for clusters" >
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159 <filter>plots_options['plots_options_selector'] == 'cluster'</filter>
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160 </data>
7
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161 <data format="tabular" name="extra_output_tab" label="$plots_options.extra_plot dataframe">
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162 <filter>plots_options['plots_options_selector'] == 'two_plot'</filter>
15
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163 </data>
7
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164 <data format="pdf" name="output_pdf" label="small RNA maps" />
0
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165 </outputs>
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166
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167 <tests>
17
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artbio
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168 <test> <!-- 0 -->
13
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169 <repeat name="series">
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170 <param name="inputs" value="input1.bam" ftype="bam" />
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171 <param name="normalization" value="1.0" />
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172 </repeat>
15
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173 <repeat name="series">
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174 <param name="inputs" value="input_new2.bam" ftype="bam" />
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175 <param name="normalization" value="2.0" />
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artbio
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176 </repeat>
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artbio
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177 <param name="minsize" value="0" />
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artbio
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178 <param name="maxsize" value="10000" />
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artbio
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179 <param name="plots_options_selector" value="one_plot" />
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artbio
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180 <param name="first_plot" value="Counts" />
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artbio
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181 <output file="input1_input2new_norm_1_2_counts.tab" name="output_tab" />
82fedc576024 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c1d96f7f028512aa4d8fcae3dd5f967cd445708e
artbio
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182 <output file="input1_input2new_norm_1_2_single_plot_counts.pdf" name="output_pdf" />
82fedc576024 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c1d96f7f028512aa4d8fcae3dd5f967cd445708e
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183 </test>
17
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artbio
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184 <test> <!-- 1 -->
15
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185 <repeat name="series">
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artbio
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186 <param name="inputs" value="input1.bam" ftype="bam" />
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artbio
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187 <param name="normalization" value="1.0" />
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artbio
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188 </repeat>
12
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artbio
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189 <param name="minsize" value="0" />
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artbio
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190 <param name="maxsize" value="10000" />
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artbio
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191 <param name="ylimits" value="yes" />
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artbio
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192 <param name="ymin" value="-5" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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193 <param name="ymax" value="5" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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194 <param name="plots_options_selector" value="one_plot" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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195 <param name="first_plot" value="Counts" />
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artbio
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196 <output file="input1_counts_yminneg5_5.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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197 <output file="input1_yminneg5_5_single_plot_counts.pdf" name="output_pdf" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
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198 </test>
17
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artbio
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199 <test> <!-- 2 -->
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artbio
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200 <repeat name="series">
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artbio
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diff changeset
201 <param name="inputs" value="input1.bam" ftype="bam" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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202 <param name="normalization" value="1.0" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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203 </repeat>
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artbio
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204 <param name="minsize" value="0" />
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artbio
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205 <param name="maxsize" value="10000" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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206 <param name="plots_options_selector" value="cluster" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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207 <param name="first_plot" value="Counts" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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208 <param name="cluster" value="5" />
20
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artbio
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209 <param name="skip_size" value="1" />
17
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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210 <param name="strandness" value="false" />
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artbio
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211 <output file="clustering.tab" name="output_tab" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
artbio
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212 <output file="clustering.pdf" name="output_pdf" />
18
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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213 <output file="bed1.bed" name="output_bed" />
17
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
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214 </test>
16
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artbio
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215 <test> <!-- 3 -->
13
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216 <repeat name="series">
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artbio
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diff changeset
217 <param name="inputs" value="input1.bam" ftype="bam" />
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218 <param name="normalization" value="1.0" />
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219 </repeat>
12
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artbio
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220 <param name="minsize" value="0" />
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artbio
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221 <param name="maxsize" value="10000" />
16
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artbio
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222 <param name="plots_options_selector" value="cluster" />
600e2498bd21 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 82bb0971cde6ba1972588c9315c3007bc3a5a6a7-dirty
artbio
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223 <param name="first_plot" value="Counts" />
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
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224 <param name="cluster" value="5" />
20
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artbio
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225 <param name="skip_size" value="1" />
17
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
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226 <param name="strandness" value="true" />
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227 <output file="clustering_unstranded.tab" name="output_tab" />
b28dcd4051e8 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 16f15e5ab2b79590a8ae410f76434aa6690c1fc4
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228 <output file="clustering_unstranded.pdf" name="output_pdf" />
18
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
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229 <output file="bed2.bed" name="output_bed" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
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230 </test>
20
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231 <test> <!-- 4 -->
18
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232 <repeat name="series">
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artbio
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233 <param name="inputs" value="input1.bam" ftype="bam" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
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234 <param name="normalization" value="1.0" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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235 </repeat>
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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diff changeset
236 <param name="minsize" value="0" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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237 <param name="maxsize" value="10000" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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diff changeset
238 <param name="plots_options_selector" value="cluster" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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diff changeset
239 <param name="first_plot" value="Counts" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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240 <param name="cluster" value="5" />
20
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artbio
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diff changeset
241 <param name="skip_size" value="2" />
18
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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242 <param name="strandness" value="false" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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243 <output file="clustering.tab" name="output_tab" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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diff changeset
244 <output file="clustering.pdf" name="output_pdf" />
2c95c899d0a4 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06c6feefeaaa66f7dec1f6143a2c5aaf91836320
artbio
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245 <output file="bed3.bed" name="output_bed" />
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
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246 </test>
20
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artbio
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247 <test> <!-- 5 -->
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artbio
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diff changeset
248 <repeat name="series">
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artbio
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249 <param name="inputs" value="input1.bam" ftype="bam" />
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artbio
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250 <param name="normalization" value="1.0" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
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251 </repeat>
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
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252 <param name="minsize" value="0" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
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253 <param name="maxsize" value="10000" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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254 <param name="plots_options_selector" value="cluster" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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255 <param name="first_plot" value="Counts" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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256 <param name="cluster" value="5" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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257 <param name="skip_size" value="2" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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258 <param name="skip_counts" value="3" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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259 <param name="skip_density" value="1.0" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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260 <param name="strandness" value="false" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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261 <output file="clustering.tab" name="output_tab" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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262 <output file="clustering.pdf" name="output_pdf" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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263 <output file="bed4.bed" name="output_bed" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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264 </test>
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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265 <test> <!-- 6 -->
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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diff changeset
266 <repeat name="series">
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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diff changeset
267 <param name="inputs" value="input1.bam" ftype="bam" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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268 <param name="normalization" value="1.0" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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269 </repeat>
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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270 <param name="minsize" value="0" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
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271 <param name="maxsize" value="10000" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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272 <param name="plots_options_selector" value="cluster" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
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273 <param name="first_plot" value="Counts" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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274 <param name="cluster" value="5" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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275 <param name="skip_size" value="2" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
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276 <param name="skip_counts" value="2" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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277 <param name="skip_density" value="0.4" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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278 <param name="strandness" value="true" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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279 <output file="clustering_unstranded.tab" name="output_tab" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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280 <output file="clustering_unstranded.pdf" name="output_pdf" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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diff changeset
281 <output file="bed5.bed" name="output_bed" />
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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diff changeset
282 </test>
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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283 <test> <!-- 7 -->
13
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
284 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
285 <param name="inputs" value="input1.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
286 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
287 </repeat>
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
288 <param name="minsize" value="20" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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289 <param name="maxsize" value="30" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
290 <param name="plots_options_selector" value="one_plot" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
291 <param name="first_plot" value="Size" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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292 <output file="input1_min20_max30_size.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
293 <output file="input1_min20_max30_single_plot_size.pdf" name="output_pdf" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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294 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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295 <test> <!-- 8 -->
13
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
296 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
297 <param name="inputs" value="input1.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
298 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
299 </repeat>
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
300 <param name="minsize" value="0" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
301 <param name="maxsize" value="10000" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
302 <param name="plots_options_selector" value="one_plot" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
303 <param name="first_plot" value="Mean" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
304 <output file="input1_mean.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
305 <output file="input1__single_plot_mean.pdf" name="output_pdf" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
306 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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307 <test> <!-- 9 -->
13
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
308 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
309 <param name="inputs" value="input1.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
310 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
311 </repeat>
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
312 <param name="minsize" value="0" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
313 <param name="maxsize" value="10000" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
314 <param name="plots_options_selector" value="one_plot" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
315 <param name="first_plot" value="Median" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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316 <output file="input1_median.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
317 <output file="input1_single_plot_median.pdf" name="output_pdf" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
318 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
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319 <test> <!-- 10 -->
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
320 <repeat name="series">
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
321 <param name="inputs" value="input1.bam" ftype="bam" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
322 <param name="normalization" value="1.0" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
323 </repeat>
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
324 <repeat name="series">
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
325 <param name="inputs" value="input2.bam" ftype="bam" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
326 <param name="normalization" value="2.0" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
327 </repeat>
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
328 <param name="minsize" value="0" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
329 <param name="maxsize" value="10000" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
330 <param name="plots_options_selector" value="one_plot" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
331 <param name="first_plot" value="Counts" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
332 <output file="input1_input2_norm_1_2_counts.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
333 <output file="input1_input2_norm_1_2_single_plot_counts.pdf" name="output_pdf" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
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diff changeset
334 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
diff changeset
335 <test> <!-- 11 -->
13
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artbio
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diff changeset
336 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
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diff changeset
337 <param name="inputs" value="input1.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
338 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
339 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
340 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
341 <param name="inputs" value="input2.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
342 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
343 </repeat>
8
1827b74f872b planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit e4588eb6c329e4516e9bcfa084a383be81b55c60
artbio
parents: 7
diff changeset
344 <param name="minsize" value="0" />
1827b74f872b planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit e4588eb6c329e4516e9bcfa084a383be81b55c60
artbio
parents: 7
diff changeset
345 <param name="maxsize" value="10000" />
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
346 <param name="ylimits" value="yes" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
347 <param name="ymin" value="-5" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
348 <param name="ymax" value="5" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
349 <param name="plots_options_selector" value="two_plot" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
350 <param name="first_plot" value="Counts" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
351 <param name="extra_plot" value="Size" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
352 <output file="input1_input2_counts.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
353 <output file="input1_input2_size.tab" name="extra_output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
354 <output file="input1_input2_double_plot_counts_size_ylimneg5_5.pdf" name="output_pdf" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
355 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
diff changeset
356 <test> <!-- 12 -->
13
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
357 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
358 <param name="inputs" value="input_single_chr.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
359 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
360 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
361 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
362 <param name="inputs" value="input_single_chr.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
363 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
364 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
365 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
366 <param name="inputs" value="input_single_chr.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
367 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
368 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
369 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
370 <param name="inputs" value="input_single_chr.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
371 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
372 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
373 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
374 <param name="inputs" value="input_single_chr.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
375 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
376 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
377 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
378 <param name="inputs" value="input_single_chr.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
379 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
380 </repeat>
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
381 <param name="minsize" value="0" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
382 <param name="maxsize" value="10000" />
9
3ea75c573429 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 6199193c7fe2cb56403eea8af0b40d44f7311fd5
artbio
parents: 8
diff changeset
383 <param name="plots_options_selector" value="one_plot" />
12
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
384 <param name="first_plot" value="Coverage" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
385 <output file="input_single_chr_x_6_single_plot_coverage.tab" name="output_tab" />
d33263e6e812 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit b0676fd329c2ca50002f9f2fede531d8e550569f
artbio
parents: 11
diff changeset
386 <output file="input_single_chr_x_6_single_plot_coverage.pdf" name="output_pdf" />
9
3ea75c573429 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 6199193c7fe2cb56403eea8af0b40d44f7311fd5
artbio
parents: 8
diff changeset
387 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
diff changeset
388 <test> <!-- 13 -->
13
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
389 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
390 <param name="inputs" value="input1.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
391 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
392 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
393 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
394 <param name="inputs" value="input2.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
395 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
396 </repeat>
9
3ea75c573429 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 6199193c7fe2cb56403eea8af0b40d44f7311fd5
artbio
parents: 8
diff changeset
397 <param name="minsize" value="0" />
3ea75c573429 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 6199193c7fe2cb56403eea8af0b40d44f7311fd5
artbio
parents: 8
diff changeset
398 <param name="maxsize" value="10000" />
7
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
399 <param name="plots_options_selector" value="global" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
400 <param name="mergestrands" value="nomerge" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
401 <param name="first_plot" value="Size" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
402 <output file="size.tab" name="output_tab" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
403 <output file="global_nomerge.pdf" name="output_pdf" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
404 </test>
20
de7fbcb1348c planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 44599c93586bc909f405ac6b745230563b290ee2
artbio
parents: 19
diff changeset
405 <test> <!-- 14 -->
13
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
406 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
407 <param name="inputs" value="input1.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
408 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
409 </repeat>
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
410 <repeat name="series">
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
411 <param name="inputs" value="input2.bam" ftype="bam" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
412 <param name="normalization" value="1.0" />
458a88317c22 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit c2fde4ca142c58719749c04046270479485e8d4b
artbio
parents: 12
diff changeset
413 </repeat>
8
1827b74f872b planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit e4588eb6c329e4516e9bcfa084a383be81b55c60
artbio
parents: 7
diff changeset
414 <param name="minsize" value="0" />
1827b74f872b planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit e4588eb6c329e4516e9bcfa084a383be81b55c60
artbio
parents: 7
diff changeset
415 <param name="maxsize" value="10000" />
7
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
416 <param name="plots_options_selector" value="global" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
417 <param name="mergestrands" value="merge" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
418 <param name="first_plot" value="Size" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
419 <output file="size.tab" name="output_tab" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
420 <output file="global_merge.pdf" name="output_pdf" />
a96e6a7df2b7 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit 06472d1bd1365e4f7b385d578a69f4646481e22f
artbio
parents: 6
diff changeset
421 </test>
0
6d48150495e3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit d4d8106d66b65679a1a685ab94bfcf99cdb7b959
artbio
parents:
diff changeset
422 </tests>
6d48150495e3 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit d4d8106d66b65679a1a685ab94bfcf99cdb7b959
artbio
parents:
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423 <help>
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424
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425 **What it does**
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426
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427 Plots mapping statistics of an alignment along the reference chromosomes :
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428
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429 - counts
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430 - mean sizes
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431 - median sizes
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432 - coverage depth
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433 - size distribution
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434
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435 Read counts, mean sizes and median sizes are computed by counting the number of 5' end of reads
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436 in each position of a chromosome reference.
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437 Coverage depths are computed from the input bam alignment files using the python pysam module.
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438
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439 The metrics mentioned above can be plotted either separately:
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440
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441 .. image:: one_plot.png
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442
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443 Or in all possible pairwise combinations:
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444
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445 .. image:: two_plot.png
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446
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447 For comparison purposes, values from bam alignment files can be normalized by a size factor
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448 before plotting.
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449
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450 **Inputs**
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451
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452 bam alignment files that must be
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453
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454 - single-read
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455 - sorted
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456 - mapped to the same reference
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457
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458 To plot 2 alignment files in the same PDF output the 'single dataset' method should be used.
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459
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460 .. class:: warningmark
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461
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462 If the 'multiple dataset' method is used the normalization factor will be applied to every file selected in the input list.
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463 Additionally each file in the selected list will be plotted in a separate PDF file.
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464
0
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465 **Output**
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466
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467 A pdf file generated by the R package lattice and one or two dataframes used to plot the data.
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468
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469 </help>
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470
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471 <citations>
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472 <citation type="doi">10.1093/bioinformatics/btp352</citation>
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473 <citation type="bibtex">@Book{,
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474 title = {Lattice: Multivariate Data Visualization with R},
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475 author = {Deepayan Sarkar},
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476 publisher = {Springer},
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477 address = {New York},
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478 year = {2008},
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479 note = {ISBN 978-0-387-75968-5},
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480 url = {http://lmdvr.r-forge.r-project.org},
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481 }</citation>
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482 </citations>
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483 </tool>