Mercurial > repos > artbio > small_rna_maps
diff test-data/test/small_rna_map.bash @ 0:6d48150495e3 draft
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_maps commit d4d8106d66b65679a1a685ab94bfcf99cdb7b959
author | artbio |
---|---|
date | Mon, 24 Jul 2017 06:28:45 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test/small_rna_map.bash Mon Jul 24 06:28:45 2017 -0400 @@ -0,0 +1,43 @@ +#! /bin/bash +# Execution command : bash small_rna_map.bash <sam file> +samtools view -h -o input.sam ../test-data/input.bam +samfile="input.sam" +cut -f2,3,4,5,6 ../test-data/output.tab > test-data.dat +grep -e "^@SQ" $samfile > header.tab +cat $samfile | perl -ne 'print unless /^@/ or ( (split /\t/)[1] != 0 and (split /\t/)[1] != 16 )' > output1.tab + +cat << EOF > pyscript.py +lendic = {} +for line in open("header.tab"): + fields = line[:-1].split() + lendic[fields[1][3:]] = fields[2][3:] +readdic = {} +for line in (open("output1.tab")): + fields = line[:-1].split() + key = "-".join([fields[2],fields[3],"F" if fields[1]=="0" else "R"]) + try: + readdic[key] += 1 + except KeyError: + readdic[key] = 1 +Out = open("output.tab", "w") +Out.write("Chromosome\tChrom_length\tCoordinate\tNbr_reads\tPolarity\n") +for key in sorted(readdic): + fields = key.split("-") + Chromosome, Coordinate, Polarity = fields[0], fields[1], fields[2] + Chrom_length = lendic[Chromosome] + Nbr_reads = str(readdic[key]) + Out.write("\t".join([Chromosome, Chrom_length, Coordinate, Nbr_reads, Polarity]) ) + Out.write("\n") +Out.close +EOF + +python ./pyscript.py +rm output1.tab header.tab pyscript.py +sort -k 1,1 -k 3,3n -k 5,5 output.tab -o output.tab +if ! diff -q output.tab test-data.dat &>/dev/null; then + >&2 echo "different" +else + >&2 echo "Test passed" +fi + +rm output.tab input.sam test-data.dat