Mercurial > repos > avowinkel > picard
diff picard_FixMateInformation.xml @ 0:5166ed57b1c4 draft
Uploaded version 1.135
author | avowinkel |
---|---|
date | Mon, 06 Jul 2015 14:46:32 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/picard_FixMateInformation.xml Mon Jul 06 14:46:32 2015 -0400 @@ -0,0 +1,81 @@ +<tool name="FixMateInformation" id="picard_FixMateInformation" version="1.135"> + <description>ensure that all mate-pair information is in sync between each read and it's mate pair</description> + <macros> + <import>picard_macros.xml</import> + </macros> + <expand macro="requirements" /> + <command> + @java_options@ + + java -jar \$JAVA_JAR_PATH/picard.jar + FixMateInformation + INPUT="${inputFile}" + OUTPUT="${outFile}" + ASSUME_SORTED=${assume_sorted} + ADD_MATE_CIGAR=${add_mate_cigar} + + SORT_ORDER=coordinate + VALIDATION_STRINGENCY="${validation_stringency}" + QUIET=true + VERBOSITY=ERROR + + </command> + <inputs> + <param name="inputFile" multiple="True" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> + <param name="add_mate_cigar" type="boolean" checked="true" truevalue="True" falsevalue="False" label="Adds the mate CIGAR tag (MC) if true, does not if false" help="ADD_MATE_CIGAR; default=True"/> + <param name="assume_sorted" type="boolean" truevalue="True" falsevalue="False" label="Assume that the input file is QUERYNAME sorted" help="ASSUME_SORTED; default=False"/> + + <expand macro="VS" /> + + </inputs> + + <outputs> + <data format="bam" name="outFile" label="${tool.name} on ${on_string}: BAM with fixed mates"/> + </outputs> + + <tests> + <test> + <param name="inputFile" value="picard_FixMateInformation.bam" ftype="bam"/> + <param name="add_mate_cigar" value="True"/> + <param name="assume_sorted" value="False"/> + <param name="validation_stringency" value="LENIENT"/> + <output name="outFile" file="picard_FixMateInformation_test1.bam" ftype="bam" lines_diff="2"/> + </test> + </tests> + + <stdio> + <exit_code range="1:" level="fatal"/> + </stdio> + + <help> + +**Purpose** + +Ensure that all mate-pair information is in sync between each read and it's mate pair. Reads marked with the secondary alignment flag are written to the output file unchanged. + +------ + +.. class:: warningmark + +**Warning on using ASSUME_SORTED option** + +Datasets imported into Galaxy are automatically coordinate sorted. So use this option (set it to True) only if you are sure that this is necessary. If you are not sure - a good rule of thumb +is to assume that the BAM you are working with is coordinate sorted. + +@dataset_collections@ + +@description@ + + ASSUME_SORTED=Boolean + AS=Boolean If true, assume that the input file is queryname sorted, even if the header says + otherwise. Default value: false. + + ADD_MATE_CIGAR=Boolean + MC=Boolean Adds the mate CIGAR tag (MC) if true, does not if false. Default value: true. + +@more_info@ + + </help> +</tool> + +