Mercurial > repos > azomics > flow_compensate
diff flow-compensate.xml @ 0:ca7ba337b727 draft
"planemo upload for repository https://github.com/AstraZeneca-Omics/immport-galaxy-tools/tree/master/flowtools/flow_compensate commit 386fcc6286d3e2d2769ac9856a653b04d5c6b0ad"
author | azomics |
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date | Tue, 25 May 2021 00:07:44 +0000 |
parents | |
children | ffc083c86d44 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/flow-compensate.xml Tue May 25 00:07:44 2021 +0000 @@ -0,0 +1,54 @@ +<tool id="flow-compensate" name="Flow compensate" version="0.1.0+galaxy0" profile="19.01" python_template_version="3.5"> + <description> + compensates a set of FCS objects through a compensation matrix + </description> + <requirements> + <requirement type="package" version="2.2.0">bioconductor-flowcore</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + mkdir -p input_dir; + #for $fcs in $fcs_list: + ln -s '$fcs' input_dir/'$fcs.element_identifier'; + #end for + mkdir -p output_dir; + Rscript '$compensate_script' '$compensation_matrix' input_dir output_dir + ]]></command> + <configfiles> + <configfile name="compensate_script"><![CDATA[ +library(flowCore) + +args <- commandArgs( TRUE ) + +if ( length( args ) != 3 ) { + cat( "ERROR: no arguments with directory, matrix file for compensation and out_dir", + file = stderr() ) + stop() +} + +comp_matrix <- read.table(file=args[[ 1 ]], sep="\t", header=TRUE, check.names = FALSE) +comp_matrix<-as.matrix(comp_matrix[,-1]) +## Square matrix, reuse colnames into rownames +rownames(comp_matrix)<-colnames(comp_matrix) +fcs_dir <- args[[ 2 ]] + +samp<-read.flowSet(files=list.files(path=fcs_dir, pattern=".*.fcs\$"), path=fcs_dir) + +samp<-compensate(samp, comp_matrix) + +write.flowSet(samp, outdir=args[[ 3 ]]) + + ]]></configfile> + </configfiles> + <inputs> + <param type="data" name="fcs_list" format="fcs" multiple="true" label="FCS files" help="FCS files to compensate using the provided matrix"/> + <param type="data" name="compensation_matrix" format="tabular" label="Matrix to use for compensation" /> + </inputs> + <outputs> + <collection name="compensated_fcs" format="fcs" label="${tool.name} compensated FCS files on ${on_string}" type="list"> + <discover_datasets pattern="__designation_and_ext__" directory="output_dir" visible="true" /> + </collection> + </outputs> + <help><![CDATA[ + TODO: Fill in help. + ]]></help> +</tool>