Mercurial > repos > azomics > flow_compensate
view flow-compensate.xml @ 0:ca7ba337b727 draft
"planemo upload for repository https://github.com/AstraZeneca-Omics/immport-galaxy-tools/tree/master/flowtools/flow_compensate commit 386fcc6286d3e2d2769ac9856a653b04d5c6b0ad"
author | azomics |
---|---|
date | Tue, 25 May 2021 00:07:44 +0000 |
parents | |
children | ffc083c86d44 |
line wrap: on
line source
<tool id="flow-compensate" name="Flow compensate" version="0.1.0+galaxy0" profile="19.01" python_template_version="3.5"> <description> compensates a set of FCS objects through a compensation matrix </description> <requirements> <requirement type="package" version="2.2.0">bioconductor-flowcore</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ mkdir -p input_dir; #for $fcs in $fcs_list: ln -s '$fcs' input_dir/'$fcs.element_identifier'; #end for mkdir -p output_dir; Rscript '$compensate_script' '$compensation_matrix' input_dir output_dir ]]></command> <configfiles> <configfile name="compensate_script"><![CDATA[ library(flowCore) args <- commandArgs( TRUE ) if ( length( args ) != 3 ) { cat( "ERROR: no arguments with directory, matrix file for compensation and out_dir", file = stderr() ) stop() } comp_matrix <- read.table(file=args[[ 1 ]], sep="\t", header=TRUE, check.names = FALSE) comp_matrix<-as.matrix(comp_matrix[,-1]) ## Square matrix, reuse colnames into rownames rownames(comp_matrix)<-colnames(comp_matrix) fcs_dir <- args[[ 2 ]] samp<-read.flowSet(files=list.files(path=fcs_dir, pattern=".*.fcs\$"), path=fcs_dir) samp<-compensate(samp, comp_matrix) write.flowSet(samp, outdir=args[[ 3 ]]) ]]></configfile> </configfiles> <inputs> <param type="data" name="fcs_list" format="fcs" multiple="true" label="FCS files" help="FCS files to compensate using the provided matrix"/> <param type="data" name="compensation_matrix" format="tabular" label="Matrix to use for compensation" /> </inputs> <outputs> <collection name="compensated_fcs" format="fcs" label="${tool.name} compensated FCS files on ${on_string}" type="list"> <discover_datasets pattern="__designation_and_ext__" directory="output_dir" visible="true" /> </collection> </outputs> <help><![CDATA[ TODO: Fill in help. ]]></help> </tool>