Mercurial > repos > azomics > flowviz_density_plots
diff FCSflowViz.xml @ 0:23c0af6be9a7 draft default tip
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/flowviz_density_plots commit 8645b278253fe79de4a23fd3e54e397bca2a9919"
author | azomics |
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date | Mon, 22 Jun 2020 20:48:47 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/FCSflowViz.xml Mon Jun 22 20:48:47 2020 -0400 @@ -0,0 +1,100 @@ +<tool id="flowviz_density_plots" name="Generate stacked density plots" version="1.0+galaxy0"> + <description>for a set of FCS files</description> + <requirements> + <requirement type="package" version="1.40.0">bioconductor-flowviz</requirement> + </requirements> + <stdio> + <exit_code range="10" level="fatal" description="Please provide a comma separated list of channels to plot" /> + <exit_code range="11" level="fatal" description="Please provide numeric values for the list of channels to plot" /> + <exit_code range="12" level="fatal" description="The set of FCS files must have the same set of channels" /> + </stdio> + <command><![CDATA[ + Rscript $__tool_directory__/FCSflowViz.R '${channels}' '${output}' '${outformat}' + #for $f in $input + '${f}' '${f.name}' + #end for + ]]> + </command> + <inputs> + <param format="fcs" name="input" type="data_collection" collection_type="list" label="FCS files Collection"/> + <param name="channels" type="text" label="Markers to plot:" value="i.e.:1,3,4" help="By default, will plot all channels."/> + <param name="outformat" type="select" label="Output Format" help="PDF will be larger files that may take some time to load."> + <option value="PNG">PNG</option> + <option value="PDF">PDF</option> + </param> + </inputs> + <outputs> + <data format="png" name="output" label="Stacked Density Plots from ${input.name} in ${outformat}"> + <change_format> + <when input="outformat" value="PDF" format="pdf" /> + </change_format> + </data> + </outputs> + <tests> + <test> + <param name="input"> + <collection type="list"> + <element name="input1.fcs" value="input1.fcs"/> + <element name="input2.fcs" value="input2.fcs"/> + <element name="input3.fcs" value="input3.fcs"/> + </collection> + </param> + <param name="channels" value="1,3"/> + <param name="outformat" value="PDF"/> + <output name="output" file="graph.pdf" compare="sim_size"/> + </test> + <test> + <param name="input"> + <collection type="list"> + <element name="input1.fcs" value="input1.fcs"/> + <element name="input2.fcs" value="input2.fcs"/> + <element name="input3.fcs" value="input3.fcs"/> + </collection> + </param> + <param name="channels" value="i.e.:1,3,4"/> + <param name="outformat" value="PNG"/> + <output name="output" file="graph.png" compare="sim_size"/> + </test> + </tests> + <help><![CDATA[ +FlowViz stacked 1D density plots +-------------------------------- + +This tool generates stacked 1D density plots using flowViz. + +**Input files** + +This tool takes a collection of valid FCS files as input. + +.. class:: warningmark + +Each FCS file in the input data collection should have the SAME set of channels. + +**Output files** + +This tool generates stacked 1D density plots for each channel. A pdf file can optionally be generated. + +class:: warningmark + +PDF are larger files that may take some time to load. It might be faster to download the PDF output once generated to open it locally. + +----- + +**Example** + +*Output*: + +.. image:: ./static/images/flowtools/flowviz.png + +----- + +**flowViz reference** + +Ellis B, Gentleman R, Hahne F, Le Meur N, Sarkar D and Jiang M (2017). flowViz: Visualization for flow cytometry. R package version 1.40.0. + + ]]> + </help> + <citations> + <citation type="doi">10.1186/1471-2105-10-106</citation> + </citations> +</tool>