comparison qiime/multiple_rarefactions.xml @ 0:003162f90751 draft

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author azuzolo
date Wed, 06 Jun 2012 16:40:30 -0400
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1 <tool id="multiple_rarefactions" name="multiple_rarefactions" version="1.2.0">
2 <description>Perform multiple subsamplings/rarefactions on an otu table</description>
3 <requirements>
4 <requirement type="binary">multiple_rarefactions.py</requirement>
5 </requirements>
6 <command interpreter="python">
7 qiime_wrapper.py
8 --galaxy_logfile=$output1
9 --galaxy_outputdir=$output1.extra_files_path
10 multiple_rarefactions.py
11 --input_path=$input_path
12 --output_path=$output1.extra_files_path
13 --min=$min
14 --max=$max
15 --step=$step
16 --num-reps=$num_reps
17 $lineages_included
18 $keep_empty_otus
19 </command>
20 <inputs>
21 <param name="input_path" type="data" label="input_path"
22 help="Input otu table filepath [REQUIRED]"/>
23 <param name="min" type="integer" value="100" label="min"
24 help="Min seqs/sample [REQUIRED]"/>
25 <param name="max" type="integer" value="400" label="max"
26 help="Max seqs/sample (inclusive) [REQUIRED]"/>
27 <param name="step" type="integer" value="100" label="step"
28 help="Size of each steps between the min/max of seqs/sample (e.g. min, min+step... for every level less than or equal to max). [REQUIRED]"/>
29 <param name="num_reps" type="integer" value="10" label="num-reps"
30 help="Number of iterations at each seqs/sample level [default: 10]"/>
31 <param name="lineages_included" type="boolean" truevalue="--lineages_included" falsevalue="" checked="false" label="lineages_included"
32 help="Output rarefied otu tables will include taxonomic (lineage) information for each otu, if present in input otu table [default: False]"/>
33 <param name="keep_empty_otus" type="boolean" truevalue="--keep_empty_otus" falsevalue="" checked="false" label="keep_empty_otus"
34 help="otus (rows) of all zeros are usually omitted from the output otu tables, with -k they will not be removed from the output files [default: False]"/>
35 </inputs>
36 <outputs>
37 <data format="txt" name="output1" label="${tool.name} on ${on_string}: Rarefied tables from $min to $max, with a step of $step"/>
38 </outputs>
39 <tests>
40 </tests>
41 <help>This tool rarefies OTU tables for use in jackknife, bootstrap, and rarefaction analyses. Samples with fewer sequences than the rarefaction depth requested for a given output otu table are omitted from those otu tables. The input is an OTU table (e.g., the output from make_otu_table). The output file is a log file listing all the rarefied otu tables produced.
42
43 For more information, see multiple_rarefactions_ in the Qiime documentation.
44
45 Updated and validated 01/16/12 by Amanda Zuzolo, Microbiome Analysis Center, George Mason University, Fairfax, VA
46
47 Qiime integration courtesy Jim Johnson, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN
48
49 .. _multiple_rarefactions: http://qiime.org/scripts/multiple_rarefactions.html</help>
50 </tool>
51