Mercurial > repos > azuzolo > qiime1_3_0
comparison qiime/pick_otus.xml @ 0:003162f90751 draft
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author | azuzolo |
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date | Wed, 06 Jun 2012 16:40:30 -0400 |
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-1:000000000000 | 0:003162f90751 |
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1 <tool id="pick_otus" name="pick_otus" version="2.0.0"> | |
2 <description>OTU picking</description> | |
3 <requirements> | |
4 <requirement type="binary">pick_otus.py</requirement> | |
5 </requirements> | |
6 <command interpreter="python"> | |
7 qiime_wrapper.py | |
8 --galaxy_outputdir='$log.extra_files_path' | |
9 #if $pick.otu_picking_method == 'uclust' and $pick.refseqs_fp.__str__ != 'None': | |
10 --galaxy_datasets='^\S+_otus\.txt$:'$otus,'^\S+_otus\.log$:'$log,'^\S+_failures\.txt$:'$failures | |
11 #else: | |
12 --galaxy_datasets='^\S+_otus\.txt$:'$otus,'^\S+_otus\.log$:'$log | |
13 #end if | |
14 pick_otus.py | |
15 --input_seqs_filepath=$input_seqs_filepath | |
16 #if $pick.otu_picking_method.__str__ == 'uclust': | |
17 #if $pick.refseqs_fp.__str__ != 'None': | |
18 --refseqs_fp=$pick.refseqs_fp | |
19 --otu_picking_method='uclust_ref' | |
20 $pick.suppress_new_clusters | |
21 #else: | |
22 --otu_picking_method=$pick.otu_picking_method | |
23 #end if | |
24 --similarity=$pick.similarity | |
25 $pick.enable_rev_strand_match | |
26 $pick.optimal_uclust | |
27 $pick.exact_uclust | |
28 $pick.user_sort | |
29 $pick.suppress_presort_by_abundance_uclust | |
30 --max_accepts=$pick.max_accepts | |
31 --max_rejects=$pick.max_rejects | |
32 #if $pick.uclust_otu_id_prefix != None and $pick.uclust_otu_id_prefix.__str__ != 'None' and $pick.uclust_otu_id_prefix.__str__ != '': | |
33 --uclust_otu_id_prefix=$pick.uclust_otu_id_prefix | |
34 #end if | |
35 $pick.uclust_stable_sort | |
36 $pick.save_uc_files | |
37 #elif $pick.otu_picking_method.__str__ == 'mothur': | |
38 --otu_picking_method=$pick.otu_picking_method | |
39 --clustering_algorithm=$pick.clustering_algorithm | |
40 --similarity=$pick.similarity | |
41 #elif $pick.otu_picking_method.__str__ == 'trie': | |
42 --otu_picking_method=$pick.otu_picking_method | |
43 $pick.trie_reverse_seqs | |
44 #elif $pick.otu_picking_method.__str__ == 'prefix_suffix': | |
45 --otu_picking_method=$pick.otu_picking_method | |
46 --prefix_length=$pick.prefix_length | |
47 --suffix_length=$pick.suffix_length | |
48 #elif pick.otu_picking_method.__str__ == 'blast': | |
49 --otu_picking_method=$pick.otu_picking_method | |
50 #if $refseqs_fp.__str__ != 'None': | |
51 --refseqs_fp=$pick.refseqs_fp | |
52 #end if | |
53 #if $pick.blast_db != None and $pick.blast_db.__str__ != 'None' and $pick.blast_db.__str__ != '': | |
54 --blast_db=$pick.blast_db | |
55 #end if | |
56 --similarity=$pick.similarity | |
57 --max_e_value=$pick.max_e_value | |
58 --min_aligned_percent=$pick.min_aligned_percent | |
59 #elif $pick.otu_picking_method == 'cdhit': | |
60 --otu_picking_method=$pick.otu_picking_method | |
61 --similarity=$pick.similarity | |
62 --max_cdhit_memory=$pick.max_cdhit_memory | |
63 #if $pick.prefix_prefilter_length != 0: | |
64 --prefix_prefilter_length=$pick.prefix_prefilter_length | |
65 #end if | |
66 $pick.trie_prefilter | |
67 #end if | |
68 --output_dir='$log.extra_files_path' | |
69 </command> | |
70 <inputs> | |
71 <param name="input_seqs_filepath" type="data" format="fasta" label="input_seqs_filepath" | |
72 help="Input sequences [REQUIRED]"/> | |
73 <conditional name="pick"> | |
74 <param name="otu_picking_method" type="select" label="otu_picking_method" | |
75 help="Method for picking OTUs. Valid choices are: mothur, trie, uclust_ref, prefix_suffix, blast, cdhit, uclust. The mothur method requires an input file of aligned sequences [default: uclust]"> | |
76 <option value="uclust" selected="true">uclust</option> | |
77 <option value="mothur">mothur</option> | |
78 <option value="trie">trie</option> | |
79 <option value="prefix_suffix">prefix_suffix</option> | |
80 <option value="blast">blast</option> | |
81 <option value="cdhit">cdhit</option> | |
82 </param> | |
83 <when value="mothur"> | |
84 <param name="clustering_algorithm" type="select" label="clustering_algorithm" | |
85 help="Clustering algorithm for mothur otu picking method. [default: furthest]"> | |
86 <option value="furthest" selected="true">furthest</option> | |
87 <option value="nearest">nearest</option> | |
88 <option value="average">average</option> | |
89 </param> | |
90 <param name="similarity" type="float" value="0.97" label="similarity" | |
91 help="Sequence similarity threshold (for cdhit, uclust, or uclust_ref) [default: 0.97]"/> | |
92 </when> <!-- mothur --> | |
93 <when value="trie"> | |
94 <param name="trie_reverse_seqs" type="boolean" truevalue="--trie_reverse_seqs" falsevalue="" checked="false" label="trie_reverse_seqs" | |
95 help="Reverse seqs before picking OTUs with the Trie OTU picker for suffix (rather than prefix) collapsing [default: False]"/> | |
96 </when> <!-- trie --> | |
97 <when value="uclust"> | |
98 <param name="refseqs_fp" type="data" format="fasta" label="refseqs_fp" optional="true" | |
99 help="Reference sequences to search against when using blast, uclust_ref, or usearch_ref [OPTIONAL]"/> | |
100 <param name="suppress_new_clusters" type="boolean" truevalue="--suppress_new_clusters" falsevalue="" checked="false" label="suppress_new_clusters" | |
101 help="Suppress creation of new clusters using seqs that don't match reference when using -m uclust_ref [default: False]"/> | |
102 <param name="suppress_presort_by_abundance_uclust" type="boolean" truevalue="--suppress_presort_by_abundance_uclust" falsevalue="" checked="false" label="suppress_presort_by_abundance_uclust" | |
103 help="Suppress presorting of sequences by abundance when picking OTUs with uclust or uclust_ref [default: False]"/> | |
104 <param name="similarity" type="float" value="0.97" label="similarity" | |
105 help="Sequence similarity threshold (for cdhit, uclust, or uclust_ref) [default: 0.97]"/> | |
106 <param name="enable_rev_strand_match" type="boolean" truevalue="--enable_rev_strand_match" falsevalue="" checked="false" label="enable_rev_strand_match" | |
107 help="Enable reverse strand matching for uclust otu picking, will double the amount of memory used. [default: False]"/> | |
108 <param name="optimal_uclust" type="boolean" truevalue="--optimal_uclust" falsevalue="" checked="false" label="optimal_uclust" | |
109 help="Pass the --optimal flag to uclust for uclust otu picking. [default: False]"/> | |
110 <param name="exact_uclust" type="boolean" truevalue="--exact_uclust" falsevalue="" checked="false" label="exact_uclust" | |
111 help="Pass the --exact flag to uclust for uclust otu picking. [default: False]"/> | |
112 <param name="user_sort" type="boolean" truevalue="--user_sort" falsevalue="" checked="false" label="user_sort" | |
113 help="Pass the --user_sort flag to uclust for uclust otu picking. [default: False]"/> | |
114 <param name="max_accepts" type="integer" value="20" label="max_accepts" | |
115 help="max_accepts value to uclust and uclust_ref [default: 20]"/> | |
116 <param name="max_rejects" type="integer" value="500" label="max_rejects" | |
117 help="max_rejects value to uclust and uclust_ref [default: 500]"/> | |
118 <param name="uclust_otu_id_prefix" type="text" label="uclust_otu_id_prefix" | |
119 help="OTU identifier prefix (string) for the de novo uclust OTU picker [default: None, OTU ids are ascending integers]"/> | |
120 <param name="uclust_stable_sort" type="boolean" truevalue="--uclust_stable_sort" falsevalue="" checked="false" label="uclust_stable_sort" | |
121 help="pass --stable_sort to uclust (uclust versions uclustq1.2.15 and later only) [default: False]"/> | |
122 <param name="save_uc_files" type="boolean" truevalue="" falsevalue="--save_uc_files" checked="false" label="save_uc_files" | |
123 help="Enable preservation of intermediate uclust (.uc) files that are used to generate clusters via uclust. [default: True]"/> | |
124 </when> <!-- uclust --> | |
125 <when value="prefix_suffix"> | |
126 <param name="prefix_length" type="integer" value="50" label="prefix_length" | |
127 help="Prefix length when using the prefix_suffix otu picker; WARNING: CURRENTLY DIFFERENT FROM prefix_prefilter_length (-n)! [default: 50]"/> | |
128 <param name="suffix_length" type="integer" value="50" label="suffix_length" | |
129 help="Suffix length when using the prefix_suffix otu picker [default: 50]"/> | |
130 | |
131 </when> <!-- prefix_suffix --> | |
132 <when value="blast"> | |
133 <param name="refseqs_fp" type="data" format="fasta" label="refseqs_fp" optional="true" | |
134 help="Reference sequences to search against"/> | |
135 <param name="blast_db" type="data" format="txt" optional="True" label="blast_db" | |
136 help="Pre-existing database to blast against when using -m blast [OPTIONAL]"/> | |
137 <param name="min_aligned_percent" type="float" value="0.5" label="min_aligned_percent" | |
138 help="Minimum percent of query sequence that can be aligned to consider a hit (BLAST OTU picker only) [default: 0.5]"/> | |
139 <param name="max_e_value" type="float" value="1e-10" label="max_e_value" | |
140 help="Max E-value when clustering with BLAST [default: 1e-10]"/> | |
141 </when> <!-- blast --> | |
142 <when value="cdhit"> | |
143 <param name="max_cdhit_memory" type="integer" value="400" label="max_cdhit_memory" | |
144 help="Maximum available memory to cd-hit-est (via the program's -M option) for cdhit OTU picking method (units of Mbyte) [default: 400]"/> | |
145 <param name="similarity" type="float" value="0.97" label="similarity" | |
146 help="Sequence similarity threshold (for cdhit, uclust, or uclust_ref) [default: 0.97]"/> | |
147 <param name="prefix_prefilter_length" type="integer" value="0" label="prefix_prefilter_length" | |
148 help="Prefilter data so seqs with identical first prefix_prefilter_length are automatically grouped into a single OTU. This is useful for large sequence collections where OTU picking doesn't scale well [default: None; 100 is a good value]"/> | |
149 <param name="trie_prefilter" type="boolean" truevalue="--trie_prefilter" falsevalue="" checked="false" label="trie_prefilter" | |
150 help="prefilter data so seqs which are identical prefixes of a longer seq are automatically grouped into a single OTU; useful for large sequence collections where OTU picking doesn't scale well [default: False]"/> | |
151 </when> <!-- cdhit --> | |
152 </conditional> <!-- pick --> | |
153 </inputs> | |
154 <outputs> | |
155 <data format="txt" name="log" label="${tool.name} on ${on_string}: log" /> | |
156 <data format="qiimeotu" name="otus" label="${tool.name} on ${on_string}: otus" /> | |
157 <data format="txt" name="failures" label="${tool.name} on ${on_string}: failures.txt" > | |
158 <filter>(pick['otu_picking_method'] == 'uclust' and pick['refseqs_fp'])</filter> | |
159 </data> | |
160 </outputs> | |
161 <tests> | |
162 </tests> | |
163 <help>For more information, see pick_otus_ in the Qiime documentation. | |
164 | |
165 Updated and validated 01/16/12 by Amanda Zuzolo, Microbiome Analysis Center, George Mason University, Fairfax, VA | |
166 | |
167 Qiime integration courtesy Jim Johnson, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN | |
168 | |
169 .. _pick_otus: http://qiime.org/scripts/pick_otus.html</help> | |
170 </tool> | |
171 |