0
|
1 Galaxy wrapper for Augustus
|
|
2 ===========================
|
|
3
|
|
4 This wrapper is copyright 2012-2013 by Björn Grüning.
|
|
5
|
|
6 This is a wrapper for the command line tool of Augustus_.
|
|
7
|
|
8 .. _augustus: http://bioinf.uni-greifswald.de/augustus/
|
|
9
|
|
10 AUGUSTUS is a program that predicts genes in eukaryotic genomic sequences.
|
|
11
|
|
12 Oliver Keller, Martin Kollmar, Mario Stanke, Stephan Waack (2011)
|
|
13 A novel hybrid gene prediction method employing protein multiple sequence alignments
|
|
14 Bioinformatics, doi: 10.1093/bioinformatics/btr010
|
|
15
|
|
16 Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008)
|
|
17 Using native and syntenically mapped cDNA alignments to improve de novo gene finding
|
|
18 Bioinformatics, doi: 10.1093/bioinformatics/btn013
|
|
19
|
|
20 Mario Stanke and Stephan Waack (2003)
|
|
21 Gene Prediction with a Hidden-Markov Model and a new Intron Submodel.
|
|
22 Bioinformatics, Vol. 19, Suppl. 2, pages ii215-ii225
|
|
23
|
|
24
|
|
25 Installation
|
|
26 ============
|
|
27
|
|
28 The recommended installation is by means of the toolshed_.
|
|
29 If you need to install it manually here is a short introduction.
|
|
30
|
1
|
31 .. _toolshed: http://toolshed.g2.bx.psu.edu/view/bgruening/augustus
|
0
|
32
|
|
33
|
|
34 Install or downlaod augustus from::
|
|
35
|
|
36 http://bioinf.uni-greifswald.de/augustus/binaries/
|
|
37
|
|
38 and follow the installation instructions or copy the binaries into your $PATH. To install the wrapper copy the augustus folder in the galaxy tools folder and modify the tools_conf.xml file to make the tool available to Galaxy.
|
|
39
|
|
40 For example::
|
|
41
|
|
42 <section name="Gene Prediction" id="gene_prediction">
|
|
43 <tool file="gene_prediction/tools/augustus/augustus.xml" />
|
|
44 </section>
|
|
45
|
|
46
|
|
47 Set the *AUGUSTUS_CONFIG_PATH* to /path_to_augustus/augustus/config with::
|
|
48
|
|
49 export AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config
|
|
50
|
|
51 or modify the wrapper and use the following additional commandline switch::
|
|
52
|
|
53 --AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config
|
|
54
|
|
55
|
|
56 History
|
|
57 =======
|
|
58
|
|
59 - v0.1: Initial public release
|
|
60 - v0.2: Added tool_dependencies.xml file and update the augustus version (thanks to James Johnson)
|
|
61 - v0.3: upgrade to augustus 2.7, added new organisms and new parameters, output additional sequence files
|
1
|
62 - v0.3.1: added parallelism and changed the output parameters from boolean to a select box
|
0
|
63
|
1
|
64 Licence (MIT)
|
|
65 =============
|
|
66
|
|
67 Permission is hereby granted, free of charge, to any person obtaining a copy
|
|
68 of this software and associated documentation files (the "Software"), to deal
|
|
69 in the Software without restriction, including without limitation the rights
|
|
70 to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
|
|
71 copies of the Software, and to permit persons to whom the Software is
|
|
72 furnished to do so, subject to the following conditions:
|
0
|
73
|
1
|
74 The above copyright notice and this permission notice shall be included in
|
|
75 all copies or substantial portions of the Software.
|
0
|
76
|
1
|
77 THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
|
|
78 IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
|
|
79 FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
|
|
80 AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
|
|
81 LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
|
|
82 OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
|
|
83 THE SOFTWARE.
|
0
|
84
|