Mercurial > repos > bgruening > bionano_scaffold
comparison test-data/configuration.xml @ 0:fec6a0b53189 draft
"planemo upload for repository https://bionanogenomics.com/support/software-downloads/ commit 1d3a1a449c7398c8fad07663224738265f77c0be"
author | bgruening |
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date | Mon, 17 May 2021 15:07:05 +0000 |
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-1:000000000000 | 0:fec6a0b53189 |
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1 <hybridScaffold> | |
2 <version> | |
3 <flag attr="version" val0="$Id: hybridScaffold_DLE1_config.xml 7702 2018-06-25 20:53:51Z apang $"/> | |
4 </version> | |
5 <global> | |
6 <flag attr="maxmem" val0="960" display="Maximum memory (GB)" group="Global options" description="Define the maximum amount of RAM in gigabytes to be used by each process."/> | |
7 <flag attr="maxthreads" val0="54" display="Max threads" group="Global options" description="Define maximum number of threads to be used by each process."/> | |
8 <flag attr="maxvirtmem" val0="960"/> | |
9 <flag attr="RAmem" val0="3" val1="1"/> | |
10 </global> | |
11 <fasta2cmap> | |
12 <flag attr="enzyme" val0="BspQI" display="Enzyme" group="FASTA to CMAP digestion" description="Define single enzyme for in-silico FASTA to CMAP digestion. Avalible enzymes: BspQI, BbvCI, BsmI, BsrDI, BssSI, DLE1."/> | |
13 <flag attr="channelNum" val0="1" display="Channel number" group="FASTA to CMAP digestion" description="Specify the channel the enzyme was used."/> | |
14 <flag attr="minLabels" val0="0" display="Minimum label sites" group="FASTA to CMAP digestion" description="Specify minimum number of label sites per digested contig."/> | |
15 <flag attr="minLength" val0="0" display="Minimum length (Kb)" group="FASTA to CMAP digestion" description="Specify minimum length in Kb of each digested contig."/> | |
16 </fasta2cmap> | |
17 <align0> | |
18 <flag attr="M" val0="1" val1="3"/> | |
19 <flag attr="ScaleDelta" val0="0.02" val1="15"/> | |
20 <flag attr="ScaleDeltaBPP"/> | |
21 <flag attr="hashScaleDelta" val0="2"/> | |
22 <flag attr="res" val0="2.9"/> | |
23 <flag attr="FP" val0="0.6"/> | |
24 <flag attr="FN" val0="0.06"/> | |
25 <flag attr="sf" val0="0.20"/> | |
26 <flag attr="sd" val0="0.0"/> | |
27 <flag attr="sr" val0="0.01"/> | |
28 <flag attr="extend" val0="1"/> | |
29 <flag attr="outlier" val0="0.0001"/> | |
30 <flag attr="endoutlier" val0="0.001"/> | |
31 <flag attr="PVendoutlier"/> | |
32 <flag attr="deltaX" val0="12"/> | |
33 <flag attr="deltaY" val0="12"/> | |
34 <flag attr="xmapchim" val0="12"/> | |
35 <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> | |
36 <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> | |
37 <flag attr="hashMultiMatch" val0="30" val1="10"/> | |
38 <flag attr="insertThreads" val0="54"/> | |
39 <flag attr="nosplit" val0="2"/> | |
40 <flag attr="biaswt" val0="0"/> | |
41 <flag attr="T" val0="1e-10" display="P-value" group="Initial alignment" description="Minimum confidence value to output initial alignments. Recommended starting value of 1e-5/genome size in Mb."/> | |
42 <flag attr="S" val0="-1000"/> | |
43 <flag attr="indel"/> | |
44 <flag attr="PVres" val0="2"/> | |
45 <flag attr="rres" val0="0.9"/> | |
46 <flag attr="MaxSE" val0="0.5"/> | |
47 <flag attr="HSDrange" val0="1.0"/> | |
48 <flag attr="outlierBC"/> | |
49 <flag attr="xmapUnique" val0="12"/> | |
50 <flag attr="AlignRes" val0="2."/> | |
51 <flag attr="outlierExtend" val0="12" val1="24"/> | |
52 <flag attr="Kmax" val0="12"/> | |
53 <flag attr="resEstimate"/> | |
54 <flag attr="f"/> | |
55 <flag attr="mres" val0="0.9"/> | |
56 </align0> | |
57 <align1> | |
58 <flag attr="res" val0="2.9"/> | |
59 <flag attr="FP" val0="0.6"/> | |
60 <flag attr="FN" val0="0.06"/> | |
61 <flag attr="sf" val0="0.20"/> | |
62 <flag attr="sd" val0="0.0"/> | |
63 <flag attr="sr" val0="0.01"/> | |
64 <flag attr="extend" val0="1"/> | |
65 <flag attr="outlier" val0="0.0001"/> | |
66 <flag attr="endoutlier" val0="0.001"/> | |
67 <flag attr="PVendoutlier"/> | |
68 <flag attr="deltaX" val0="12"/> | |
69 <flag attr="deltaY" val0="12"/> | |
70 <flag attr="xmapchim" val0="12"/> | |
71 <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> | |
72 <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> | |
73 <flag attr="hashMultiMatch" val0="30" val1="10"/> | |
74 <flag attr="insertThreads" val0="54"/> | |
75 <flag attr="nosplit" val0="2"/> | |
76 <flag attr="biaswt" val0="0"/> | |
77 <flag attr="T" val0="1e-10" display="P-value" group="Initial alignment" description="Minimum confidence value to output initial alignments. Recommended starting value of 1e-5/genome size in Mb."/> | |
78 <flag attr="S" val0="-1000"/> | |
79 <flag attr="indel"/> | |
80 <flag attr="PVres" val0="2"/> | |
81 <flag attr="rres" val0="0.9"/> | |
82 <flag attr="MaxSE" val0="0.5"/> | |
83 <flag attr="HSDrange" val0="1.0"/> | |
84 <flag attr="outlierBC"/> | |
85 <flag attr="xmapUnique" val0="12"/> | |
86 <flag attr="AlignRes" val0="2."/> | |
87 <flag attr="outlierExtend" val0="12" val1="24"/> | |
88 <flag attr="Kmax" val0="12"/> | |
89 <flag attr="resEstimate"/> | |
90 <flag attr="f"/> | |
91 <flag attr="mres" val0="0.9"/> | |
92 </align1> | |
93 <assignAlignType> | |
94 <flag attr="T_cutoff" val0="1e-11" display="P-value" group="Chimeric/conflicting alignment flagging" description="Minimum confidence value used to flag chimeric/conflicting alignments. Recommand to set it to be the same as the merge_Tvalue below."/> | |
95 <flag attr="max_overhang" val0="5" display=""/> | |
96 </assignAlignType> | |
97 <cut_conflicts> | |
98 <flag attr="window_size" val0="10000" display="Distance (bp)" group="Conflict-cut" description="The distance (bp) from a conflicting site within which the chimeric quality score of BioNano genome map labels will be examined"/> | |
99 <flag attr="min_quality_score_threshold" val0="35" display="Percent (%)" group="Conflict-cut" description="The minimal percentage of molecules spanning to the left and right of a label of interest, thus supporting the BioNano assembly at that region"/> | |
100 <flag attr="min_coverage_threshold" val0="10" display="Coverage (X)" group="Conflict-cut" description="The minimal number of molecules aligning to a label of interest in the BioNano assembly"/> | |
101 </cut_conflicts> | |
102 <mergeNGS_BN> | |
103 <flag attr="merge_Tvalue" val0="1e-11" display="P-value" group="Merging" description="Minimum confidence value used to merge alignments. Recommand to set it to be the same as the assignAlignType T_cutoff above"/> | |
104 <flag attr="id_shift" val0="100000" display="BioNano cmap id shift" group="Merging" description="Value to shift the BioNano cmap id to distinguish the BioNano cmaps from sequence cmaps. Recommand to set it to be greater than the number of sequence entries."/> | |
105 <flag attr="max_merge_rounds" val0="40" display=""/> | |
106 <flag attr="endoutlier" val0="1e-4" display=""/> | |
107 <flag attr="outlier" val0="1e-4" display=""/> | |
108 <flag attr="biaswt" val0="0" display=""/> | |
109 <flag attr="sd" val0="0.1" display=""/> | |
110 <flag attr="res" val0="2.9" display=""/> | |
111 <flag attr="mres" val0="2.9" display=""/> | |
112 <flag attr="sf" val0="0.2" display=""/> | |
113 <flag attr="RepeatMask" val0="4" val1="0.01" display=""/> | |
114 <flag attr="RepeatRec" val0="0.7" val1="0.6" val2="1.4" display=""/> | |
115 <flag attr="pairmerge" val0="80" val1="0.2" display="Min alignment length and Max endoutlier" group="Merging" description="Minimum alignment length required for pair merge, and the maximum endoutlier allowed."/> | |
116 <flag attr="maxmem" val0="960" display=""/> | |
117 <flag attr="pairmergeRepeat"/> | |
118 <flag attr="NoBpp"/> | |
119 <flag attr="first" val0="-1" display=""/> | |
120 <flag attr="f"/> | |
121 </mergeNGS_BN> | |
122 <align_final_1st_pass> | |
123 <flag attr="res" val0="2.9"/> | |
124 <flag attr="FP" val0="0.6"/> | |
125 <flag attr="FN" val0="0.06"/> | |
126 <flag attr="sf" val0="0.20"/> | |
127 <flag attr="sd" val0="0.0"/> | |
128 <flag attr="sr" val0="0.01"/> | |
129 <flag attr="extend" val0="1"/> | |
130 <flag attr="outlier" val0="0.0001"/> | |
131 <flag attr="endoutlier" val0="0.001"/> | |
132 <flag attr="PVendoutlier"/> | |
133 <flag attr="deltaX" val0="12"/> | |
134 <flag attr="deltaY" val0="12"/> | |
135 <flag attr="xmapchim" val0="12"/> | |
136 <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> | |
137 <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> | |
138 <flag attr="hashMultiMatch" val0="30" val1="10"/> | |
139 <flag attr="insertThreads" val0="54"/> | |
140 <flag attr="nosplit" val0="2"/> | |
141 <flag attr="biaswt" val0="0"/> | |
142 <flag attr="T" val0="1e-10" display="P-value" group="Initial alignment" description="Minimum confidence value to output intial alignments. Recommended starting value of 1e-5/genome size in Mb."/> | |
143 <flag attr="S" val0="-1000"/> | |
144 <flag attr="indel"/> | |
145 <flag attr="PVres" val0="2"/> | |
146 <flag attr="rres" val0="0.9"/> | |
147 <flag attr="MaxSE" val0="0.5"/> | |
148 <flag attr="HSDrange" val0="1.0"/> | |
149 <flag attr="outlierBC"/> | |
150 <flag attr="xmapUnique" val0="12"/> | |
151 <flag attr="AlignRes" val0="2."/> | |
152 <flag attr="outlierExtend" val0="6" val1="24"/> | |
153 <flag attr="Kmax" val0="6"/> | |
154 <flag attr="resEstimate"/> | |
155 <flag attr="f"/> | |
156 <flag attr="mres" val0="0.9"/> | |
157 <flag attr="MultiMatches" val0="5"/> | |
158 </align_final_1st_pass> | |
159 <align_final_2nd_pass> | |
160 <flag attr="res" val0="2.9"/> | |
161 <flag attr="FP" val0="0.6"/> | |
162 <flag attr="FN" val0="0.06"/> | |
163 <flag attr="sf" val0="0.20"/> | |
164 <flag attr="sd" val0="0.0"/> | |
165 <flag attr="sr" val0="0.01"/> | |
166 <flag attr="extend" val0="1"/> | |
167 <flag attr="outlier" val0="0.0001"/> | |
168 <flag attr="endoutlier" val0="0.001"/> | |
169 <flag attr="PVendoutlier"/> | |
170 <flag attr="deltaX" val0="12"/> | |
171 <flag attr="deltaY" val0="12"/> | |
172 <flag attr="xmapchim" val0="12"/> | |
173 <flag attr="hashgen" val0="5" val1="3" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> | |
174 <flag attr="hash" val0="-hashdelta" val1="50"/> | |
175 <flag attr="hashMultiMatch" val0="30" val1="10" val2="3"/> | |
176 <flag attr="insertThreads" val0="54"/> | |
177 <flag attr="nosplit" val0="2"/> | |
178 <flag attr="biaswt" val0="0"/> | |
179 <flag attr="T" val0="1e-10" display="P-value" group="Final alignment" description="Minimum confidence score used to align NGS contigs back to hybrid scaffold."/> | |
180 <flag attr="S" val0="-1000"/> | |
181 <flag attr="indel"/> | |
182 <flag attr="PVres" val0="2"/> | |
183 <flag attr="rres" val0="0.9"/> | |
184 <flag attr="MaxSE" val0="0.5"/> | |
185 <flag attr="HSDrange" val0="1.0"/> | |
186 <flag attr="outlierBC"/> | |
187 <flag attr="xmapUnique" val0="12"/> | |
188 <flag attr="AlignRes" val0="2."/> | |
189 <flag attr="outlierExtend" val0="12" val1="24"/> | |
190 <flag attr="Kmax" val0="12"/> | |
191 <flag attr="resEstimate"/> | |
192 <flag attr="f"/> | |
193 <flag attr="mres" val0="0.9"/> | |
194 <flag attr="MultiMatches" val0="5"/> | |
195 </align_final_2nd_pass> | |
196 <align_final_BNG> | |
197 <flag attr="res" val0="2.9"/> | |
198 <flag attr="FP" val0="0.6"/> | |
199 <flag attr="FN" val0="0.06"/> | |
200 <flag attr="sf" val0="0.20"/> | |
201 <flag attr="sd" val0="0.0"/> | |
202 <flag attr="sr" val0="0.01"/> | |
203 <flag attr="extend" val0="1"/> | |
204 <flag attr="outlier" val0="0.0001"/> | |
205 <flag attr="endoutlier" val0="0.001"/> | |
206 <flag attr="PVendoutlier"/> | |
207 <flag attr="deltaX" val0="6"/> | |
208 <flag attr="deltaY" val0="6"/> | |
209 <flag attr="xmapchim" val0="12"/> | |
210 <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> | |
211 <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> | |
212 <flag attr="hashMultiMatch" val0="30" val1="10"/> | |
213 <flag attr="insertThreads" val0="4"/> | |
214 <flag attr="nosplit" val0="2"/> | |
215 <flag attr="biaswt" val0="0"/> | |
216 <flag attr="T" val0="1e-10" display="P-value" group="Final alignment" description="Minimum confidence score used to align NGS contigs back to hybrid scaffold."/> | |
217 <flag attr="S" val0="-1000"/> | |
218 <flag attr="indel"/> | |
219 <flag attr="PVres" val0="2"/> | |
220 <flag attr="rres" val0="0.9"/> | |
221 <flag attr="MaxSE" val0="0.5"/> | |
222 <flag attr="HSDrange" val0="1.0"/> | |
223 <flag attr="outlierBC"/> | |
224 <flag attr="xmapUnique" val0="14"/> | |
225 <flag attr="AlignRes" val0="2."/> | |
226 <flag attr="outlierExtend" val0="6" val1="24"/> | |
227 <flag attr="Kmax" val0="6"/> | |
228 <flag attr="resEstimate"/> | |
229 <flag attr="BestRef" val0="1"/> | |
230 <flag attr="f"/> | |
231 <flag attr="mres" val0="0.9"/> | |
232 </align_final_BNG> | |
233 <refineFinal1> | |
234 <flag attr="refine" val0="1" display="Refine Map" group="Final Refinement" default0="3"/> | |
235 <flag attr="usecolor" val0="1" default0="1" display="Color Channel" group="Final Refinement"/> | |
236 <flag attr="A" val0="5" display="Aligned Sites Threshold" group="Final Refinement" default0="5"/> | |
237 <flat attr="S" val0="-9"/> | |
238 <flag attr="L" val0="130"/> | |
239 <flag attr="maptype" val0="0"/> | |
240 <flag attr="extend" val0="1" display="Allow Extended Molecule" group="Final Refinement" default0="1"/> | |
241 <flag attr="MaxCov" val0="100" display="Max Coverage" group="Final Refinement" default0="100"/> | |
242 <flag attr="Mprobeval" display="Fast Mode" group="Final Refinement"/> | |
243 <flag attr="splitcnt"/> | |
244 <flag attr="splitrev" val0="2"/> | |
245 <flag attr="CmapSNR"/> | |
246 <flag attr="splitsite"/> | |
247 <flag attr="MinSplitLen" val0="50.0"/> | |
248 <flag attr="MaxSE" val0="0.5"/> | |
249 <flag attr="outlier" val0="2e-2" display="Min Outliers P-value" group="Second Refinement" default0="1e-5"/> | |
250 <flag attr="outlierMax" val0="80."/> | |
251 <flag attr="outlierLambda" val0="20."/> | |
252 <flag attr="endoutlier" val0="1e-3" display="Molecule Ends P-value Cutoff" group="Second Refinement" default0="1e-4"/> | |
253 <flag attr="endoutlierRef" val0="1e-4" val1="1e-3"/> | |
254 <flag attr="skip_dist" val0="0.0" val2="0.0"/> | |
255 <flag attr="endoutlierFinal" val0="1"/> | |
256 <flag attr="maxEnd" val0="90."/> | |
257 <flag attr="RTHETA_FIX" val0="0"/> | |
258 <flag attr="TB" val0="1e-6" val1="1e-6" val2="-TBmult" val3="0.1"/> | |
259 <flag attr="nosplit" val0="2" display="Allow Chimeric Split" group="Final Refinement" default0="2"/> | |
260 <flag attr="EndTrim" val0="0.0" display="Min End Trim Coverage" group="Final Refinement" default0="4.99"/> | |
261 <flag attr="biaswt" val0="0.7" display="Bias" group="Final Refinement" default0="0"/> | |
262 <flag attr="biaswtEnd" val0="0.0"/> | |
263 <flag attr="biaswtRefine" val0="0.7" val2="1"/> | |
264 <flag attr="biaswtOutlier" val0="0.0"/> | |
265 <flag attr="LRbias" val0="1e0" display="Soft Threshold" group="Final Refinement" default0="1e2"/> | |
266 <flag attr="deltaX" val0="6" display="Molecule Labels Metric" group="Final Refinement" default0="4"/> | |
267 <flag attr="deltaY" val0="7" display="Mapped Labels Metric" group="Final Refinement" default0="6"/> | |
268 <flag attr="RepeatMask" val0="5" val1="0.01" display="Repeat Mask P-values" group="Final Refinement" default0="2" default1="0.01"/> | |
269 <flag attr="RepeatRec" val0="0.7" val1="0.6" val2="1.4"/> | |
270 <flag attr="CovTrim" val0="2"/> | |
271 <flag attr="CovTrimLen" val0="55"/> | |
272 <flag attr="TrimNorm" val0="0"/> | |
273 <flag attr="TrimNormMed" val0="100"/> | |
274 <flag attr="TrimNormChim" val0="2"/> | |
275 <flag attr="TrimNormMin" val0="2"/> | |
276 <flag attr="PVres" val0="2"/> | |
277 <flag attr="PVendoutlier"/> | |
278 <flag attr="AlignRes" val0="1.5"/> | |
279 <flag attr="rres" val0="1.2"/> | |
280 <flag attr="cres" val0="5.6" val1="3" val2="0.1"/> | |
281 <flag attr="hashgen" val00="5" val01="3" val02="2.4" val03="1.5" val04="0.05" val05="5.0" val06="1" val07="1" val08="3"/> | |
282 <flag attr="hash" val00="-hashdelta" val01="10" val02="10" val03="-mres" val04="0.9"/> | |
283 <flag attr="HSDrange" val0="1.0"/> | |
284 <flag attr="hashoffset" val0="1"/> | |
285 <flag attr="hashMultiMatch" val0="15"/> | |
286 <flag attr="insertThreads" val0="54"/> | |
287 <flag attr="BestRef" val0="1"/> | |
288 <flag attr="BestRefPV" val0="1"/> | |
289 <flag attr="ChimQuality"/> | |
290 <flag attr="f" display="Overwrite Output Files" group="Final Refinement"/> | |
291 <flag attr="T" val0="1e-11" display="P Value Cutoff Threshold"/> | |
292 </refineFinal1> | |
293 </hybridScaffold> |