Mercurial > repos > bgruening > bismark
comparison documentation/readme.rst @ 7:fcadce4d9a06 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit b'e6ee273f75fff61d1e419283fa8088528cf59470\n'
author | bgruening |
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date | Sat, 06 May 2017 13:18:09 -0400 |
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1 =============== | |
2 Bismark Wrapper | |
3 =============== | |
4 | |
5 Bismark_ uses Bowtie or Bowtie2 to map bisulfite converted sequence reads to a reference genome and determine cytosine methylation states. | |
6 | |
7 Publication: http://www.ncbi.nlm.nih.gov/pubmed/21493656 | |
8 | |
9 User Guide: http://www.bioinformatics.babraham.ac.uk/projects/bismark/Bismark_User_Guide_v0.7.12.pdf | |
10 | |
11 .. _bismark: http://www.bioinformatics.babraham.ac.uk/projects/bismark/ | |
12 | |
13 Preparation | |
14 =========== | |
15 | |
16 Create your reference index with *bismark_genome_preparation* in your normal Galaxy Bowtie2/Botwie index directory. It will create a Bisulfite_Genome folder directly in your Bowtie2/Bowtie index directory. | |
17 If you follow that approach you do not need to specify or modify an extra .loc file. | |
18 That wrapper will extract the path to the Bisulfite_Genome folder from ./tool-data/bowtie2_indices.loc or ./tool-data/bowtie_indices.loc. | |
19 | |
20 ======= | |
21 History | |
22 ======= | |
23 | |
24 - v0.7: Initial public release | |
25 - v0.7.8: update and add Tool Shed Integration | |
26 - v0.7.11.1 change default output to BAM, from now on samtools are required | |
27 - v0.7.11.2 added multi-threading to samtools (samtools > 0.1.19 is required) | |
28 - v0.7.12 upgrade to bismark 0.7.12 and fix a major slowdown | |
29 - v0.7.12.1 define a dependency to samtools 0.1.19 | |
30 | |
31 | |
32 =============================== | |
33 Wrapper Licence (MIT/BSD style) | |
34 =============================== | |
35 | |
36 Permission to use, copy, modify, and distribute this software and its | |
37 documentation with or without modifications and for any purpose and | |
38 without fee is hereby granted, provided that any copyright notices | |
39 appear in all copies and that both those copyright notices and this | |
40 permission notice appear in supporting documentation, and that the | |
41 names of the contributors or copyright holders not be used in | |
42 advertising or publicity pertaining to distribution of the software | |
43 without specific prior permission. | |
44 | |
45 THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL | |
46 WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED | |
47 WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE | |
48 CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT | |
49 OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS | |
50 OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE | |
51 OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE | |
52 OR PERFORMANCE OF THIS SOFTWARE. | |
53 |