Mercurial > repos > bgruening > bismark
diff bismark_deduplicate/bismark_deduplicate_wrapper.xml @ 7:fcadce4d9a06 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit b'e6ee273f75fff61d1e419283fa8088528cf59470\n'
author | bgruening |
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date | Sat, 06 May 2017 13:18:09 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bismark_deduplicate/bismark_deduplicate_wrapper.xml Sat May 06 13:18:09 2017 -0400 @@ -0,0 +1,69 @@ +<tool id="bismark_deduplicate" name="Bismark Deduplicate" version="0.16.3"> + + <description>Deduplicates reads mapped by Bismark</description> + <!--<version_command>bismark version</version_command>--> + + <requirements> + <requirement type="package" version="0.1.19">samtools</requirement> + <requirement type="package" version="2.1.0">bowtie2</requirement> + </requirements> + + <stdio> + <exit_code range="1:" /> + <exit_code range=":-1" /> + <regex match="Error:" /> + <regex match="Exception:" /> + </stdio> + + <command interpreter="python"> +<![CDATA[ + bismark_deduplicate_wrapper.py + + --tool_dir "$__tool_directory__" + + #if str ( $sPaired ) == "single": + -s + #else + -p + #end if + + --input "$mapping_output" + + --output_report "$output_report" + --output_bam "$output_bam" + + ##--log_report "$log_report" +]]> + </command> + + <inputs> + <param name="sPaired" type="select" label="Is this library mate-paired?" format="bam"> + <option value="single">Single-end</option> + <option value="paired">Paired-end</option> + </param> + <param name="mapping_output" type="data" format="bam, sam" label="Submit the resulting bam/sam file from Bismark bisulfite mapper" /> + </inputs> + + <outputs> + <data name="output_bam" format="bam" label="${tool.name} on ${on_string}: deduplicated mapped reads" /> + <data name="output_report" format="txt" label="${tool.name} on ${on_string}: deduplication report"/> + <!--<data name="log_report" format="txt" label="${tool.name} on ${on_string}: log report (tool stdout)"/>--> + </outputs> + + <help> +<![CDATA[ +**What it does** + + | This tool is supposed to remove alignments to the same position in the genome from the Bismark mapping output (both single and paired-end SAM files), which can arise by e.g. excessive PCR amplification. If sequences align to the same genomic position but on different strands they will be scored individually. + | + | Note that deduplication is not recommended for RRBS-type experiments! + | + | For single-end alignments only use the start-coordinate of a read will be used for deduplication. + | For paired-end alignments the start-coordinate of the first read and the end coordinate of the second read will be used for deduplication. + +]]> + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btr167</citation> + </citations> +</tool> \ No newline at end of file