Mercurial > repos > bgruening > bismark
view bismark_deduplicate_wrapper.py @ 21:120b7b35e442 draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit 8fdc76a99a9dcf34549898a208317607afd18798"
author | bgruening |
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date | Thu, 22 Apr 2021 17:05:46 +0000 |
parents | 9bfe38410155 |
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#!/usr/bin/python import argparse import logging import os import shutil import signal import subprocess import sys import tempfile def stop_err(logger, msg): logger.critical(msg) sys.exit(1) def restore_sigpipe(): """ Needed to handle samtools view """ signal.signal(signal.SIGPIPE, signal.SIG_DFL) def log_subprocess_output(logger, pipe): for line in iter(pipe.readline, b""): logger.debug(line.decode().rstrip()) def get_arg(): parser = argparse.ArgumentParser() parser.add_argument("--single_or_paired", dest="single_or_paired") parser.add_argument("--input", dest="input", metavar="input") parser.add_argument( "--output_report", dest="output_report", metavar="output_report" ) parser.add_argument("--output_bam", dest="output_bam", metavar="output_report") parser.add_argument( "--log_report", dest="log_report", metavar="log_filename", type=str ) args = parser.parse_args() return args def __main__(): args = get_arg() logger = logging.getLogger("bismark_deduplicate_wrapper") logger.setLevel(logging.DEBUG) ch = logging.StreamHandler(sys.stdout) if args.log_report: ch.setLevel(logging.WARNING) handler = logging.FileHandler(args.log_report) handler.setLevel(logging.DEBUG) logger.addHandler(handler) else: ch.setLevel(logging.DEBUG) logger.addHandler(ch) # ensure the input has a .bam suffix tmp_dir = tempfile.mkdtemp(prefix="tmp", suffix="") os.chdir(tmp_dir) default_reads_name = "submitted_reads.bam" os.symlink(args.input, default_reads_name) single_or_paired = "-s" if args.single_or_paired == "single" else "-p" cmd = ["deduplicate_bismark", single_or_paired, default_reads_name, "--bam"] logger.info("Deduplicating with: '%s'", " ".join(cmd)) process = subprocess.Popen( cmd, stdout=subprocess.PIPE, stderr=subprocess.STDOUT, preexec_fn=restore_sigpipe, ) proc_out, proc_err = process.communicate() logger.info(proc_out) if process.returncode != 0: stop_err( logger, "Bismark deduplication error (also check the log file if any)!\n%s" % proc_err, ) deduplicated_out_name = "submitted_reads.deduplicated.bam" deduplicated_report_name = "submitted_reads.deduplication_report.txt" logger.debug("Moving '%s' to galaxy: '%s'.", deduplicated_out_name, args.output_bam) shutil.move(deduplicated_out_name, args.output_bam) logger.debug( "Moving '%s' to galaxy: '%s'.", deduplicated_report_name, args.output_report ) shutil.move("submitted_reads.deduplication_report.txt", args.output_report) logger.debug("Done.") if __name__ == "__main__": __main__()