Mercurial > repos > bgruening > bismark
view bismark_deduplicate/bismark_deduplicate_wrapper.py @ 7:fcadce4d9a06 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit b'e6ee273f75fff61d1e419283fa8088528cf59470\n'
author | bgruening |
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date | Sat, 06 May 2017 13:18:09 -0400 |
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#!/usr/bin/python import argparse import os import re import shutil import subprocess import sys import tempfile import logging from glob import glob def cleanup_before_exit(tmp_dir): if tmp_dir and os.path.exists(tmp_dir): shutil.rmtree(tmp_dir) def get_arg(): parser = argparse.ArgumentParser() parser.add_argument('--tool_dir', dest='tool_dir', action='store', nargs=1, metavar='tool_dir', type=str) parser.add_argument('-p', action='store_true') parser.add_argument('-s', action='store_true') parser.add_argument('--input', dest='input', action='store', nargs=1, metavar='input', type=str) parser.add_argument('--output_report', dest='output_report', action='store', nargs=1, metavar='output_report', type=str) parser.add_argument('--output_bam', dest='output_bam', action='store', nargs=1, metavar='output_report', type=str) parser.add_argument('--log_report', dest='log_report', action='store', nargs=1, metavar='log_filename', type=str) args = parser.parse_args() return args def __main__(): args = get_arg() tmp_dir = tempfile.mkdtemp(prefix='tmp', suffix='') os.chdir(tmp_dir) if args.log_report: logging.basicConfig(level=logging.INFO, filename=args.log_report[0], filemode="a+", format='%(message)s') else: logging.basicConfig(level=logging.INFO, filename=os.path.join(tmp_dir, 'log_report.txt'), filemode="a+", format='%(message)s') default_reads_name = 'submitted_reads.bam' os.symlink(args.input[0], default_reads_name) if args.p is True: sPaired = '-p' if args.s is True: sPaired = '-s' cmd = 'perl %s %s duplicated_reads.bam --bam' % (os.path.join(args.tool_dir[0], 'deduplicate_bismark'), sPaired) logging.info('COMMAND LINE:\n\n%s' % cmd) proc = subprocess.Popen(['perl', os.path.join(args.tool_dir[0], 'deduplicate_bismark'), sPaired, default_reads_name, '--bam'], stdout=subprocess.PIPE, stderr=subprocess.STDOUT) proc_out, proc_err = proc.communicate() logging.info("__________________________________________________________________\n") logging.info("BISMARK DEDUPLICATE STDOUT:\n\n%s" % proc_out) if proc_err: logging.critical("__________________________________________________________________\n") logging.critical("BISMARK DEDUPLICATE WARNING:\n\n%s" % proc_err) sys.exit("Dedpulicate Bismark crashed with the folowing error message:\n%s" % proc_err) shutil.move( glob('*deduplicated.bam')[0], args.output_bam[0] ) shutil.move( glob('*deduplication_report.txt')[0], args.output_report[0]) cleanup_before_exit(tmp_dir) if __name__=="__main__": __main__()