Mercurial > repos > bgruening > cp_cellprofiler
view track_objects.py @ 7:eb6c08714244 draft default tip
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
author | bgruening |
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date | Sun, 05 Nov 2023 09:28:09 +0000 |
parents | 0e4dccaafef5 |
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#!/usr/bin/env python import argparse import json from cp_common_functions import (get_json_value, get_pipeline_lines, get_total_number_of_modules, INDENTATION, update_module_count, write_pipeline) MODULE_NAME = "TrackObjects" OUTPUT_FILENAME = "output.cppipe" def build_header(module_name, module_number): result = "|".join( [ f"{module_name}:[module_num:{module_number}", "svn_version:\\'Unknown\\'", "variable_revision_number:7", "show_window:True", "notes:\\x5B\\'Track the embryos across images using the Overlap method\\x3A tracked objects are identified by the amount of frame-to-frame overlap. Save an image of embryos labeled with a unique number across time.\\'\\x5D", "batch_state:array(\\x5B\\x5D, dtype=uint8)", "enabled:True", "wants_pause:False]\n", ] ) return result def build_main_block(input_params): result = INDENTATION.join( [ f"{INDENTATION}Choose a tracking method:{get_json_value(input_params,'con_tracking_method.tracking_method')}\n", f"Select the objects to track:{get_json_value(input_params,'object_to_track')}\n", ] ) tracking_method = get_json_value( input_params, "con_tracking_method.tracking_method" ) obj_measurement = "None" # default value if tracking_method == "Measurements": measurement_category = get_json_value( input_params, "con_tracking_method.con_measurement_category.measurement_category", ) measurement = get_json_value( input_params, "con_tracking_method.con_measurement_category.measurement" ) if measurement_category == "Intensity" or measurement_category == "Location": img_measure = get_json_value( input_params, "con_tracking_method.con_measurement_category.img_measure" ) obj_measurement = f"{measurement_category}_{measurement}_{img_measure}" else: obj_measurement = f"{measurement_category}_{measurement}" result += INDENTATION.join( [ f"{INDENTATION}Select object measurement to use for tracking:{obj_measurement}\n" ] ) if tracking_method == "LAP": # no max distance required, set default for pipeline max_distance = 50 else: max_distance = get_json_value(input_params, "con_tracking_method.max_distance") result += INDENTATION.join( [f"{INDENTATION}Maximum pixel distance to consider matches:{max_distance}\n"] ) display_option = get_json_value(input_params, "con_tracking_method.display_option") output_img_name = "TrackedCells" # default value, required by cppipe regardless of its presence in UI save = get_json_value( input_params, "con_tracking_method.con_save_coded_img.save_coded_img" ) if save == "Yes": output_img_name = get_json_value( input_params, "con_tracking_method.con_save_coded_img.name_output_img" ) result += INDENTATION.join( [ f"{INDENTATION}Select display option:{display_option}\n", f"Save color-coded image?:{save}\n", f"Name the output image:{output_img_name}\n", ] ) # LAP method default values movement_model = "Both" no_std = 3.0 radius_limit_max = 10.0 radius_limit_min = 2.0 radius = "2.0,10.0" run_second = "Yes" gap_closing = 40 split_alt = 40 merge_alt = 40 max_gap_displacement = 5 max_split = 50 max_merge = 50 max_temporal = 5 max_mitosis_dist = 40 mitosis_alt = 80 # LAP method if tracking_method == "LAP": movement_model = get_json_value( input_params, "con_tracking_method.movement_method" ) no_std = get_json_value(input_params, "con_tracking_method.no_std_radius") radius_limit_max = get_json_value( input_params, "con_tracking_method.max_radius" ) radius_limit_min = get_json_value( input_params, "con_tracking_method.min_radius" ) radius = f"{radius_limit_min},{radius_limit_max}" run_second = get_json_value( input_params, "con_tracking_method.con_second_lap.second_lap" ) if run_second == "Yes": gap_closing = get_json_value( input_params, "con_tracking_method.con_second_lap.gap_closing" ) split_alt = get_json_value( input_params, "con_tracking_method.con_second_lap.split_alt" ) merge_alt = get_json_value( input_params, "con_tracking_method.con_second_lap.merge_alt" ) max_gap_displacement = get_json_value( input_params, "con_tracking_method.con_second_lap.max_gap_displacement" ) max_split = get_json_value( input_params, "con_tracking_method.con_second_lap.max_split" ) max_merge = get_json_value( input_params, "con_tracking_method.con_second_lap.max_merge" ) max_temporal = get_json_value( input_params, "con_tracking_method.con_second_lap.max_temporal" ) max_mitosis_dist = get_json_value( input_params, "con_tracking_method.con_second_lap.max_mitosis_distance" ) mitosis_alt = get_json_value( input_params, "con_tracking_method.con_second_lap.mitosis_alt" ) result += INDENTATION.join( [ f"{INDENTATION}Select the movement model:{movement_model}\n", f"Number of standard deviations for search radius:{no_std}\n", f"Search radius limit, in pixel units (Min,Max):{radius}\n", f"Run the second phase of the LAP algorithm?:{run_second}\n", f"Gap closing cost:{gap_closing}\n", f"Split alternative cost:{split_alt}\n", f"Merge alternative cost:{merge_alt}\n", f"Maximum gap displacement, in pixel units:{max_gap_displacement}\n", f"Maximum split score:{max_split}\n", f"Maximum merge score:{max_merge}\n", f"Maximum temporal gap, in frames:{max_temporal}\n", ] ) # common section filter_by_lifetime = get_json_value( input_params, "con_tracking_method.con_filter_by_lifetime.filter_by_lifetime" ) use_min = "Yes" # default min_life = 1 # default use_max = "No" # default max_life = 100 # default if filter_by_lifetime == "Yes": use_min = get_json_value( input_params, "con_tracking_method.con_filter_by_lifetime.con_use_min.use_min", ) if use_min == "Yes": min_life = get_json_value( input_params, "con_tracking_method.con_filter_by_lifetime.con_use_min.min_lifetime", ) use_max = get_json_value( input_params, "con_tracking_method.con_filter_by_lifetime.con_use_max.use_max", ) if use_max == "Yes": max_life = get_json_value( input_params, "con_tracking_method.con_filter_by_lifetime.con_use_max.max_lifetime", ) result += INDENTATION.join( [ f"{INDENTATION}Filter objects by lifetime?:{filter_by_lifetime}\n", f"Filter using a minimum lifetime?:{use_min}\n", f"Minimum lifetime:{min_life}\n", f"Filter using a maximum lifetime?:{use_max}\n", f"Maximum lifetime:{max_life}\n", ] ) # print 2 leftover from LAP result += INDENTATION.join( [ f"{INDENTATION}Mitosis alternative cost:{mitosis_alt}\n", f"Maximum mitosis distance, in pixel units:{max_mitosis_dist}\n", ] ) # Follow Neighbors # defaults avg_cell_diameter = 35.0 use_adv = "No" cost_of_cell = 15.0 weight_of_area_diff = 25.0 if tracking_method == "Follow Neighbors": avg_cell_diameter = get_json_value( input_params, "con_tracking_method.avg_diameter" ) use_adv = get_json_value( input_params, "con_tracking_method.con_adv_parameter.adv_parameter" ) if use_adv == "Yes": cost_of_cell = get_json_value( input_params, "con_tracking_method.con_adv_parameter.cost" ) weight_of_area_diff = get_json_value( input_params, "con_tracking_method.con_adv_parameter.weight" ) result += INDENTATION.join( [ f"{INDENTATION}Average cell diameter in pixels:{avg_cell_diameter}\n", f"Use advanced configuration parameters:{use_adv}\n", f"Cost of cell to empty matching:{cost_of_cell}\n", f"Weight of area difference in function matching cost:{weight_of_area_diff}\n", ] ) result = result.rstrip("\n") return result if __name__ == "__main__": parser = argparse.ArgumentParser() parser.add_argument("-p", "--pipeline", help="CellProfiler pipeline") parser.add_argument("-i", "--inputs", help="JSON inputs from Galaxy") args = parser.parse_args() pipeline_lines = get_pipeline_lines(args.pipeline) inputs_galaxy = json.load(open(args.inputs, "r")) current_module_num = get_total_number_of_modules(pipeline_lines) current_module_num += 1 pipeline_lines = update_module_count(pipeline_lines, current_module_num) header_block = build_header(MODULE_NAME, current_module_num) main_block = build_main_block(inputs_galaxy) module_pipeline = f"\n{header_block}{main_block}\n" pipeline_lines.append(module_pipeline) write_pipeline(OUTPUT_FILENAME, pipeline_lines)