Mercurial > repos > bgruening > deeptools
diff bamCoverage.xml @ 28:f7712a057440 draft
new bugfix release
author | bgruening |
---|---|
date | Wed, 02 Apr 2014 09:15:44 -0400 |
parents | bf1b1dcdd67b |
children | 3a2aab18a217 |
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--- a/bamCoverage.xml Mon Mar 17 16:23:58 2014 -0400 +++ b/bamCoverage.xml Wed Apr 02 09:15:44 2014 -0400 @@ -1,4 +1,4 @@ -<tool id="deeptools_bamCoverage" name="bamCoverage" version="1.0.5"> +<tool id="deeptools_bamCoverage" name="bamCoverage" version="@WRAPPER_VERSION@.0"> <description> generates a coverage bigWig file from a given BAM file. Multiple options are available to count reads and normalize coverage. (bam2bigwig)</description> <expand macro="requirements" /> <expand macro="stdio" /> @@ -133,11 +133,15 @@ **What it does** -Given a BAM file, this tool generates a bigWig or bedGraph file with genome-wide coverage of fragment or read coverages. -The way the method works is by first calculating all the number of reads (either extended to match the fragment length or not) -that overlap each bin (a region of fixed length, i.e. 25 bp) in the genome. Bins with zero counts are skipped, i.e. not added to the output file. -The resulting read counts can be normalized using either a given scaling factor, the RPKM formula or to get a 1x depth of coverage (RPGC). - +Given a BAM file, this tool generates a bigWig or bedGraph file of fragment or +read coverages. The way the method works is by first calculating all the +number of reads (either extended to match the fragment length or not) that +overlap each bin in the genome. The resulting read counts can be normalized +using either a given scaling factor, the RPKM formula or to get a 1x depth of +coverage (RPGC). In the case of paired-end mapping each read mate is treated +independently to avoid a bias when a mixture of concordant and discordant +pairs is present. This means that *each end* will be extended to match the +fragment length. .. image:: $PATH_TO_IMAGES/norm_IGVsnapshot_indFiles.png