Mercurial > repos > bgruening > deeptools_multi_bam_summary
diff multiBamSummary.xml @ 1:f2a582e0314d draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit fef8b344925620444d93d8159c0b2731a5777920
author | bgruening |
---|---|
date | Mon, 15 Feb 2016 10:27:26 -0500 |
parents | 04b612e24e62 |
children | b319edc108a7 |
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--- a/multiBamSummary.xml Mon Jan 25 20:18:00 2016 -0500 +++ b/multiBamSummary.xml Mon Feb 15 10:27:26 2016 -0500 @@ -41,14 +41,14 @@ </command> <inputs> - <expand macro="multiple_input_bams" /> + <expand macro="multiple_input_bams_min2" /> <conditional name="mode"> - <param name="modeOpt" type="select" label="Choose computation mode" + <param name="modeOpt" type="select" label="Choose computation mode" help="In the bins mode, the coverage is computed for equally - sized bins. In the BED file mode, a list of genomic regions in BED - format has to be given. For each region in the BED file the number of - overlapping reads is counted in each of the BAM files. + sized bins. \nIn BED file mode, a list of genomic regions + in BED or INTERVAL format has to be given. For each region + in the BED file, the number of overlapping reads from each BAM file is counted. "> <option value="bins" selected="true">Bins</option> <option value="BED-file">Limit calculation to certain regions (BED file)</option> @@ -63,7 +63,7 @@ </when> <when value="BED-file"> <param name="region_file" type="data" format="bed" - label="Region file in BED format" + label="Region file in BED or INTERVAL format" help="Coverage is computed for the number of reads that overlap such regions."/> </when> </conditional> @@ -102,26 +102,27 @@ <help> <![CDATA[ -**What it does** +What it does +------------- -This tool generates a matrix of read-coverages for a list of genomic regions and at least two samples (BAM files). -The genome is split into bins of the given size. For each bin, the number of reads found in it in each BAM file is counted. -Alternatively, a bed file with pre-defined genomic regions can be provided. In each case the calculation can further be limited to -a given genomic interval (e.g. a given chromosome). This option is mostly used for testing and efficiency. -A typical follow-up application is to check and visualize the similarity between replicates or published data sets (see: plotPCA and plotCorrelation). +This tool generates a matrix of read coverages for a list of genomic regions and at least two samples (BAM files). +The genome is split into bins of the given size. For each bin, the number of reads found in each BAM file is counted. +Alternatively, an interval file with pre-defined genomic regions can be provided. -**Output files**: +In principle, this tool does the same as ``multiBigwigSummary``, but for BAM files. + +A typical follow-up application is to check and visualize the similarity and variability between replicates or published data sets (see: ``plotPCA`` and ``plotCorrelation``). -- **score matrix**: a compressed matrix where every row corresponds to a genomic region (or bin) and each column corresponds to a sample (BAM file) -- Optional : Uncompressed **score matrix**, in case you want to analyse the coverage scores yourself. (Select to "Save raw counts" from above) +Output +-------- -======= +The default output is a **compressed file** that can only be used with ``plotPCA`` or ``plotCorrelation``. -.. image:: $PATH_TO_IMAGES/QC_multiBamSummary_humanSamples.png - :alt: Heatmap of RNA Polymerase II ChIP-seq +To analyze the coverage scores yourself, you can get the **uncompressed score matrix** where every row corresponds to a genomic region (or bin) and each column corresponds to a sample (BAM file). (To obtain this output file, select "Save raw counts (coverages) to file" ) - -You can find more details on the multiBamSummary doc page: https://deeptools.readthedocs.org/en/master/content/tools/multiBamSummary.html +.. image:: $PATH_TO_IMAGES/multiBamSummary_output.png + :width: 600 + :height: 443 -----