annotate plotProfiler.xml @ 23:1d290f98916a draft

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1 <tool id="deeptools_plot_profile" name="plotProfile" version="@WRAPPER_VERSION@.0" profile="18.01">
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2 <description>
1
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3 creates a profile plot for score distributions across genomic regions
0
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4 </description>
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5 <macros>
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6 <token name="@BINARY@">plotProfile</token>
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7 <import>deepTools_macros.xml</import>
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8 </macros>
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9 <expand macro="requirements" />
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10 <command>
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11 <![CDATA[
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12 @BINARY@
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13 --matrixFile "$matrixFile"
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14 --outFileName "$outFileName"
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15
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16 #if $output.showOutputSettings == "yes"
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17 --plotFileFormat $output.outFileFormat
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18
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19 #if $output.dpi:
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20 --dpi '$output.dpi'
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21 #end if
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22
0
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23 #if $output.saveSortedRegions:
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24 --outFileSortedRegions '$outFileSortedRegions'
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25 #end if
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26 #else
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27 --plotFileFormat 'png'
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28 #end if
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29
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30 #if $scaleRegions.showScaleRegionsOpt == "yes":
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31 --startLabel '$scaleRegions.startLabel'
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32 --endLabel '$scaleRegions.endLabel'
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33 #end if
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34
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35 #if $advancedOpt.showAdvancedOpt == "yes":
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36 #if $advancedOpt.averageType:
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37 --averageType '$advancedOpt.averageType'
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38 #end if
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39 --plotHeight $advancedOpt.plotHeight
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40 --plotWidth $advancedOpt.plotWidth
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41 --plotType $advancedOpt.plotType
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42 #if $advancedOpt.samplesLabel and str($advancedOpt.samplesLabel).strip() != "":
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43 --samplesLabel $advancedOpt.samplesLabel
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44 #end if
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45
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46 #if $advancedOpt.regionsLabel and str($advancedOpt.regionsLabel).strip() != "":
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47 --regionsLabel $advancedOpt.regionsLabel
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48 #end if
0
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49
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50 #if $advancedOpt.plotTitle and str($advancedOpt.plotTitle).strip() != "":
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51 --plotTitle '$advancedOpt.plotTitle'
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52 #end if
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53
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54 #if str($advancedOpt.colors).strip() != "":
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55 --colors #echo ' '.join( ["'%s'" % $color for $color in $advancedOpt.colors.split()] )#
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56 #end if
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57
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58 --legendLocation '$advancedOpt.legendLocation'
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59
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60 --labelRotation '$advancedOpt.labelRotation'
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61
0
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62 $advancedOpt.perGroup
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63
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64 #if str($advancedOpt.yMin):
0
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65 --yMin $advancedOpt.yMin
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66 #end if
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67 #if str($advancedOpt.yMax):
0
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68 --yMax $advancedOpt.yMax
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69 #end if
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70
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71 #if $advancedOpt.outFileNameData:
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72 --outFileNameData '$output_outFileNameData'
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73 #end if
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74
0
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75 @KMEANS_CLUSTERING@
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76
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77 #end if
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78 ]]>
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79 </command>
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80 <inputs>
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81 <param argument="--matrixFile" format="deeptools_compute_matrix_archive" type="data" label="Matrix file from the computeMatrix tool"/>
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82 <conditional name="scaleRegions">
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83 <param name="showScaleRegionsOpt" type="select" label="The input matrix was computed in scale-regions mode">
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84 <option value="no" selected="true">no</option>
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85 <option value="yes">yes</option>
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86 </param>
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87 <when value="no" />
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88 <when value="yes">
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89 <param argument="--startLabel" type="text" value="TSS" size="10"
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90 label="Label for the region start"
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91 help ="Label shown in the plot
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92 for the start of the region. Default is TSS (transcription start site),
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93 but could be changed to anything, e.g. &quot;peak start&quot;." />
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94 <param argument="--endLabel" type="text" value="TES" size="10"
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95 label="Label for the region end"
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96 help="Label shown in the plot for the region end. Default is TES (transcription end site)."/>
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97 </when>
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98 </conditional>
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99
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100 <expand macro="input_graphic_output_settings">
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101 <expand macro="input_image_file_format" />
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102 <expand macro="output_dpi" />
0
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103 </expand>
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104
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105 <conditional name="advancedOpt">
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106 <param name="showAdvancedOpt" type="select" label="Show advanced options" >
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107 <option value="no" selected="true">no</option>
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108 <option value="yes">yes</option>
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109 </param>
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110 <when value="no" />
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111 <when value="yes">
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112 <param name="averageType" type="select" label="Define the type of statistic that should be used for the profile.">
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113 <option value="mean" selected="true">mean</option>
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114 <option value="median">median</option>
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115 <option value="min">min</option>
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116 <option value="max">max</option>
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117 <option value="sum">sum</option>
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118 <option value="std">std</option>
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119 </param>
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120 <param argument="--plotHeight" type="integer" value="5" min="3"
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121 label="Plot height"
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122 help="Height in cm. The default for the plot height is 5 centimeters. The minimum value is 3 cm." />
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123
1
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124 <param argument="--plotWidth" type="integer" value="11" min="1"
0
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125 label="Plot width"
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126 help="Width in cm. The default value is 11 centimeters. The minimum value is 1 cm." />
0
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127
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128 <param argument="--plotType" type="select" label="Plot type"
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129 help="The &quot;lines&quot; option will
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130 plot the profile line based on the average type selected. The &quot;fill&quot;
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131 option fills the region between zero and the profile curve. The filled in color is
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132 semi-transparent. The &quot;add standard error&quot;
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133 option colors the region between the profile and the standard error of the data.
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134 As in the case of fill, a semi-transparent color is used.
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135 The option &quot;overlapped_lines&quot; plots each region's value, one on top of
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136 the other.">
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137 <option value="lines" selected="true">lines</option>
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138 <option value="fill">fill</option>
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139 <option value="se">add standard error</option>
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140 <option value="std">add standard deviation</option>
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141 <option value="overlapped_lines">overlapped lines</option>
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142 <option value="heatmap">heatmap</option>
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143 </param>
1
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144 <param argument="--samplesLabel" type="text" size="30"
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145 label="Labels for the samples (each bigwig) plotted"
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146 help="The default is to use the file name of the sample. The sample labels should be separated by
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147 spaces and quoted if a label itself contains a space, e.g., label-1 &quot;label 2&quot;">
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148 <sanitizer>
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149 <valid initial="string.printable">
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150 </valid>
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151 </sanitizer>
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152 </param>
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153 <param argument="--regionsLabel" type="text" size="30"
0
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154 label="Labels for the regions plotted in the heatmap"
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155 help="If more than one region is being plotted a list of labels separated by space is required.
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156 If a label itself contains a space, then quotes are needed.
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157 For example, label_1 &quot;label 2&quot;" />
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158
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159 <expand macro="plotTitle" />
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160 <param argument="--colors" type="text" value="" size="40"
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161 label="List of colors to use for the plotted lines"
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162 help="Color names and html hex strings (e.g. #eeff22) are accepted.
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163 The color names should be separated by spaces. (--colors red blue green)">
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164 <validator type="expression"
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165 message="Only numbers, digits, '#' and spaces are allowed.">all(c in ' #abcdefghijklmnopqrstuvwxyz0123456789' for c in value)</validator>
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166 </param>
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167 <expand macro="legendLocation" />
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168 <expand macro="labelRotation" />
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169
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170 <param argument="--perGroup" type="boolean" truevalue="--perGroup" falsevalue=""
1
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171 label="Make one plot per group of regions"
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172 help="The default is to make one plot per bigWig file, i.e., all samples next to each other. Choosing this option will make one plot per group of regions. " />
0
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173
3
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174 <param argument="--outFileNameData" type="boolean" label="Save the data underlying the average profile"
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175 help="This option will create an additional output file." />
0
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176
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177 <param argument="--yMin" type="float" value="" size="3" optional="true"
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178 label="Minimum value for the Y-axis of the summary plot. Leave empty for automatic values"/>
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179 <param argument="--yMax" type="float" value="" size="3" optional="true"
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180 label="Maximum value for Y-axis of the summary plot. Leave empty for automatic values" />
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181
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182 <expand macro="kmeans_clustering" />
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183
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184 </when>
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185 </conditional>
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186 </inputs>
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187 <outputs>
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188 <expand macro="output_image_file_format" />
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189 <expand macro="output_graphic_outputs" />
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190
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191 <data format="tabular" name="output_outFileNameData" label="${tool.name} on ${on_string}: Underlying data">
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192 <filter>advancedOpt['showAdvancedOpt'] == "yes" and advancedOpt['outFileNameData'] is True</filter>
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193 </data>
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194
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195 </outputs>
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196 <tests>
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197 <test>
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198 <param name="matrixFile" value="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" />
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199 <output name="outFileName" file="profiler_result1.png" ftype="png" compare="sim_size" delta="4000" />
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200 </test>
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201 <test>
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202 <param name="matrixFile" value="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" />
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203 <param name="showAdvancedOpt" value="yes" />
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204 <param name="showScaleRegionsOpt" value="yes" />
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205 <param name="outFileNameData" value="True" />
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206 <output name="outFileName" file="profiler_result2.png" ftype="png" compare="sim_size" delta="4000" />
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207 <output name="output_outFileNameData" file="profiler_result2.tabular" ftype="tabular" />
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208 </test>
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209 </tests>
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210 <help>
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211 <![CDATA[
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212
1
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213 What it does
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214 -------------
0
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215
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216 This tool plots the average enrichments over all genomic
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217 regions supplied to ``computeMarix``. It requires that ``computeMatrix`` was successfully run.
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218 It is a very useful complement to ``plotHeatmap``, especially in cases where you want to
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219 compare the scores for many different groups. Like ``plotHeatmap``, ``plotProfile`` does not change the
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220 values that were computed by ``computeMatrix``, but you can modify the color and other display properties of the plots.
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221
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222 Output
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223 -------------
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224
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225 .. image:: $PATH_TO_IMAGES/plotProfiler_examples.png
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226 :width: 600
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227 :height: 858
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228
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229 =======
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230
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231 In addition to the image, ``plotProfile`` can also generate the values underlying the profile.
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232
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233 See the following table for the **optional output** options:
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234
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235 +-----------------------------------+--------------------+-----------------+-----------------+
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236 | **optional output type** | **computeMatrix** | **plotHeatmap** | **plotProfile** |
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237 +-----------------------------------+--------------------+-----------------+-----------------+
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238 | values underlying the heatmap | yes | yes | no |
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239 +-----------------------------------+--------------------+-----------------+-----------------+
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240 | values underlying the profile | no | no | yes |
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241 +-----------------------------------+--------------------+-----------------+-----------------+
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242 | sorted and/or filtered regions | yes | yes | yes |
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243 +-----------------------------------+--------------------+-----------------+-----------------+
0
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244
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245 -----
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246
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247 @REFERENCES@
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248 ]]>
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249 </help>
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250 <expand macro="citations" />
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251 </tool>