Mercurial > repos > bgruening > diamond
annotate diamond.xml @ 6:64be1ac21109 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
author | bgruening |
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date | Thu, 27 Sep 2018 06:30:30 -0400 |
parents | 830516f9521b |
children | 62c9df8382c2 |
rev | line source |
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6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
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changeset
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1 <tool id="bg_diamond" name="Diamond" version="@VERSION@.0"> |
0 | 2 <description>alignment tool for short sequences against a protein database</description> |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
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changeset
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3 <macros> |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
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changeset
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4 <import>macros.xml</import> |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
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5 </macros> |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
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6 <expand macro="requirements" /> |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
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diff
changeset
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7 <expand macro="stdio" /> |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
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8 <expand macro="version_command" /> |
0 | 9 <command> |
10 <![CDATA[ | |
11 | |
12 #if $ref_db_source.db_source == "history": | |
13 ln -s $ref_db_source.reference_database ./database.dmnd | |
14 #else: | |
15 ln -s ${ref_db_source.index.fields.db_path} ./database.dmnd | |
16 #end if | |
17 | |
18 && | |
19 | |
20 diamond | |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
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diff
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21 $method_cond.method_select |
0 | 22 --threads "\${GALAXY_SLOTS:-12}" |
23 --db ./database | |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
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24 --query '$query' |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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25 #if $method_cond.method_select == "blastx" |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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26 --query-gencode '$method_cond.query_gencode' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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27 --strand '$method_cond.query_strand' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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28 --min-orf $method_cond.min_orf |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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29 #if $method_cond.frameshift_cond.frameshift_select == 'yes' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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30 --frameshift $method_cond.frameshift_cond.frameshift |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
changeset
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31 $method_cond.frameshift_cond.range_culling |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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32 #end if |
3
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
parents:
1
diff
changeset
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33 #end if |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
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34 |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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35 @OUTPUT_ARGS@ |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
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36 |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
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37 --compress '0' |
3
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
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changeset
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38 #if $sensitivity == "1" |
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
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39 --sensitive |
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
parents:
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diff
changeset
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40 #else if $sensitivity == "2" |
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
parents:
1
diff
changeset
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41 --more-sensitive |
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
parents:
1
diff
changeset
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42 #end if |
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
parents:
1
diff
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43 |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
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44 #if str($gapopen) != "": |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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45 --gapopen '$gapopen' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
changeset
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46 #end if |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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47 #if str($gapextend) != "": |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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48 --gapextend '$gapextend' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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49 #end if |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
parents:
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diff
changeset
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50 --matrix '$matrix' |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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51 --comp-based-stats '$comp_based_stats' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
changeset
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52 --masking '$masking' |
0 | 53 |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
3
diff
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54 @HITFILTER_ARGS@ |
0 | 55 |
56 #if str($filter_score.filter_score_select) == 'evalue': | |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
parents:
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diff
changeset
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57 --evalue '$filter_score.evalue' |
0 | 58 #else: |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
parents:
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diff
changeset
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59 --min-score '$filter_score.min_score' |
0 | 60 #end if |
61 | |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
parents:
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diff
changeset
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62 --id '$id' |
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
parents:
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diff
changeset
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63 --query-cover '$query_cover' |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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64 --subject-cover '$subject_cover' |
1
df7738595640
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
bgruening
parents:
0
diff
changeset
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65 --block-size '$block_size' |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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66 #if str($unal) == '1': |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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67 --unal 1 --un '$unalqueries' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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68 #end if |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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69 $no_self_hits |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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70 #if $tax_cond.tax_select == 'file': |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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71 --taxonlist `cat '$tax_cond.taxonlistfile' | grep -v "^#" | grep -v "^$" | tr "\n" "," | sed 's/,$//'` |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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72 #else if $tax_cond.tax_select == 'list': |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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73 --taxonlist '$tax_cond.taxonlist' |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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74 #end if |
0 | 75 ]]> |
76 </command> | |
77 <inputs> | |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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diff
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78 <conditional name="method_cond"> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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79 <param name="method_select" type="select" label="What do you want to align?" help="(blastp/blastx)"> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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80 <option value="blastp">Align amino acid query sequences (blastp)</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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81 <option value="blastx">Align DNA query sequences (blastx)</option> |
3
830516f9521b
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 0a893f1ee7f73d24004a43ec1ba6a4cc03fbfab0
bgruening
parents:
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82 </param> |
6
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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83 <when value="blastx"> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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84 <param name="query_gencode" argument="--query-gencode" type="select" label="Genetic code used for translation of query in BLASTX mode" help=""> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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85 <option value="1">The Standard Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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86 <option value="2">The Vertebrate Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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87 <option value="3">The Yeast Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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88 <option value="4">The Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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89 <option value="5">The Invertebrate Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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90 <option value="6">The Ciliate, Dasycladacean and Hexamita Nuclear Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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91 <option value="9">The Echinoderm and Flatworm Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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92 <option value="10">The Euplotid Nuclear Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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93 <option value="11">The Bacterial, Archaeal and Plant Plastid Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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94 <option value="12">The Alternative Yeast Nuclear Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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95 <option value="13">The Ascidian Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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96 <option value="14">The Alternative Flatworm Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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97 <option value="16">Chlorophycean Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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98 <option value="21">Trematode Mitochondrial Code</option> |
64be1ac21109
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
bgruening
parents:
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99 <option value="22">Scenedesmus obliquus Mitochondrial Code</option> |
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100 <option value="23">Thraustochytrium Mitochondrial Code</option> |
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101 <option value="24">Pterobranchia Mitochondrial Code</option> |
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102 <option value="25">Candidate Division SR1 and Gracilibacteria Code</option> |
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103 <option value="26">Pachysolen tannophilus Nuclear Code</option> |
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104 </param> |
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105 <param argument="--min-orf" name="min_orf" type="integer" value="1" label="ignore translated sequences without an open reading frame of at least this length" help="By default this feature is disabled for sequences of length below 30, set to 20 for sequences of length below 100, and set to 40 otherwise. Setting this option to 1 will disable this feature" /> |
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106 |
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107 <param name="query_strand" argument="--strand" type="select" label="query strands to search" help=""> |
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108 <option value="both" selected="True">Both</option> |
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109 <option value="plus">Plus</option> |
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110 <option value="minus">Minus</option> |
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111 </param> |
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112 <conditional name="frameshift_cond"> |
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113 <param name="frameshift_select" type="select" label="Allow for frameshifts?" help=""> |
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114 <option value="yes">yes</option> |
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115 <option value="no" selected="true">no</option> |
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116 </param> |
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117 <when value="yes"> |
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118 <param argument="--range-culling" name="range_culling" type="boolean" truevalue="--range-culling" falsevalue="" checked="false" label="restrict hit culling to overlapping query ranges" help="This feature is designed for long query DNA sequences that may span several genes. In these cases, the default of reporting the 25 best overall hits could cause hits to a lower scoring gene to be overshadowed. But just increasing the number of alignments reported will bloat the output size and reduce performance. Using this feature along with -k 25 (default), a hit will only be deleted if at least 50% of its query range is spanned by at least 25 higher or equal scoring hits. Using this feature along with --top 10, a hit will only be deleted if its score is more than 10% lower than that of a higher scoring hit over at least 50% of its query range. The percentage is configurable using --range-cover. Note that this feature is currently only available in frameshift alignment mode"/> |
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119 <param argument="--frameshift" type="integer" value="0" label="frame shift penalty" help="Values around 15 are reasonable for this parameter. Enabling this feature will have the aligner tolerate missing bases in DNA sequences and is most recommended for long, error-prone sequences like MinION reads. In the pairwise output format, frameshifts will be indicated by \ and / for a shift by +1 and -1 nucleotide in the direction of translation respectively." /> |
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120 </when> |
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121 <when value="no"/> |
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122 </conditional> |
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123 </when> |
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124 <when value="blastp"> |
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125 </when> |
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126 </conditional> |
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127 <param argument="--query" type="data" format="fasta,fastq" label="Input query file in FASTA or FASTQ format" /> |
0 | 128 <conditional name="ref_db_source"> |
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129 <param name="db_source" type="select" label="Will you select a reference database from your history or use a built-in index?" help="Built-ins were indexed using default options"> |
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130 <option value="indexed">Use a built-in index</option> |
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131 <option value="history">Use one from the history</option> |
0 | 132 </param> |
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133 <when value="indexed"> |
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134 <param name="index" type="select" label="Select a reference database" help="If your database of interest is not listed, contact your Galaxy admin"> |
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135 <options from_data_table="diamond_database"> |
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136 <filter type="sort_by" column="2"/> |
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137 <validator type="no_options" message="No indexes are available for the selected input dataset"/> |
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138 </options> |
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139 </param> |
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140 </when> |
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141 <when value="history"> |
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142 <param name="reference_database" type="data" format="dmnd" label="Select the reference database" /> |
0 | 143 </when> |
144 </conditional> | |
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145 <expand macro="output_type_macro" /> |
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146 <param name="no_self_hits" argument="--no-self-hits" type="boolean" truevalue="--no-self-hits" falsevalue="" checked="true" label="suppress reporting of identical self hits?" help=""/> |
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147 <param name='sensitivity' type="select" label="Sensitivity Mode" help="Choose one of the sensitivity modes. The default mode is mainly designed for short read alignment, i.e. finding significant matches of >50 bits on 30-40aa fragments. The sensitive mode is a lot more sensitive than the default and generally recommended for aligning longer sequences. The more sensitive mode provides even more sensitivity. More sensitivity may increase computation time."> |
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148 <option value="0" selected="True">Default</option> |
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149 <option value="1">Sensitive</option> |
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150 <option value="2">More Sensitive</option> |
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151 </param> |
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152 <param argument="--matrix" type="select" label="Scoring matrix" help="In parentheses are the supported values for (gap open)/(gap extend). In brackets are default gap penalties"> |
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153 <option value="BLOSUM45">BLOSUM45 ((10-13)/3; (12-16)/2; (16-19)/1) [14/2]</option> |
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154 <option value="BLOSUM50">BLOSUM50 ((9-13)/3; (12-16)/2; (15-19)/1) [13/2]</option> |
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155 <option value="BLOSUM62" selected="True">BLOSUM62 ((6-11)/2; (9-13)/1) [11/1]</option> |
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156 <option value="BLOSUM80">BLOSUM80 ((6-9)/2; 13/2; 25/2; (9-11)/1) [10/1]</option> |
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157 <option value="BLOSUM90">BLOSUM90 ((6-9)/2; (9-11)/1) [10/1]</option> |
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158 <option value="PAM250">PAM250 ((11-15)/3; (13-17)/2; (17-21)/1) [14/2]</option> |
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159 <option value="PAM70">PAM70 ((6-8)/2; (9-11)/1) [10/1]</option> |
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160 <option value="PAM30">PAM30 ((5-7)/2; (8-10)/1) [9/1]</option> |
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161 </param> |
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162 <param argument="--gapopen" type="integer" optional="True" value="" label="Gap open penalty" help="leave empty for default (see scoring matrix)" /> |
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163 <param argument="--gapextend" type="integer" optional="True" value="" label="Gap extension penalty" help="leave empty for default (see scoring matrix)" /> |
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164 <param name="comp_based_stats" argument="--comp-based-stats" type="boolean" truevalue="1" falsevalue="0" checked="true" label="enable composition based statistics?" help="Compositionally biased sequences often cause false positive matches, which are effectively filtered by this algorithm in a way similar to the composition based statistics used by BLAST"/> |
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165 <param argument="--masking" type="boolean" truevalue="1" falsevalue="0" checked="true" label="enable masking of low complexity regions?" help="Masked residues appear in the output as X"/> |
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166 <conditional name="tax_cond"> |
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167 <param name="tax_select" type="select" label="Restrict search taxonomically?" help="Any taxonomic rank can be used, and only reference sequences matching one of the specified taxon ids will be searched against"> |
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168 <option value="no">No</option> |
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169 <option value="list">list of taxids entered manually</option> |
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170 <option value="file">list of taxids from single column tabular file</option> |
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171 </param> |
6
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172 <when value="no"/> |
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173 <when value="list"> |
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174 <param name="taxonlist" argument="--taxonlist" type="text" value="" label="comma separated list of taxon ids" help="" /> |
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175 </when> |
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176 <when value="file"> |
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177 <param name="taxonlistfile" argument="--taxonlist" type="data" format="tabular" label="Keep alignments within the given percentage range of the top alignment score for a quer" help="" /> |
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178 </when> |
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179 </conditional> |
0 | 180 <conditional name="filter_score"> |
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181 <param name="filter_score_select" type="select" label="Method to filter?" help="(--evalue/--min-score)"> |
0 | 182 <option value="evalue">Maximum e-value to report alignments</option> |
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183 <option value="min-score">Minimum bit score to report alignments</option> |
0 | 184 </param> |
185 <when value="evalue"> | |
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186 <param argument="--evalue" type="float" value="0.001" label="Maximum expected value to keep an alignment" /> |
0 | 187 </when> |
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188 <when value="min-score"> |
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189 <param name="min_score" argument="--min-score" type="integer" value="0" label="Minimum bit score to keep an alignment" help="(--min-score)" /> |
0 | 190 </when> |
191 </conditional> | |
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192 <expand macro="hit_filter_macro" /> |
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193 <param argument="--id" type="integer" value="0" label="Minimum identity percentage to report an alignment" help="" /> |
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194 <param name="query_cover" argument="--query-cover" type="integer" value="0" label="Minimum query cover percentage to report an alignment" help="" /> |
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195 <param name="subject_cover" argument="--subject-cover" type="integer" value="0" label="Minimum subject cover percentage to report an alignment" help="" /> |
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196 <param name="block_size" argument="--block-size" type="float" value="2" label="Block size in billions of sequence letters to be processed at a time" help="" /> |
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197 <param argument="--unal" type="boolean" truevalue="1" falsevalue="0" checked="false" label="report unaligned queries" help=""/> |
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198 </inputs> |
0 | 199 <outputs> |
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200 <expand macro="output_macro" /> |
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201 <data format="fasta" name="unalqueries" label="${tool.name} on ${on_string} (unaligned queries)"> |
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202 <filter>unal == "1"</filter> |
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203 </data> |
0 | 204 </outputs> |
205 <tests> | |
206 <test> | |
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207 <conditional name="method_cond"> |
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208 <param name="method_select" value="blastp" /> |
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209 </conditional> |
0 | 210 <param name="query" value="protein.fasta" ftype="fasta"/> |
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211 <conditional name="ref_db_source"> |
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212 <param name="db_source" value="history"/> |
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213 <param name="reference_database" value="db.dmnd"/> |
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214 </conditional> |
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215 <conditional name="output"> |
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216 <param name="outfmt" value="6"/> |
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217 <param name="fields" value="qseqid,sseqid,pident,length,mismatch,gapopen,qstart,qend,sstart,send,evalue,bitscore"/> |
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218 </conditional> |
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219 <param name="sensitivity" value="0"/> |
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220 <param name="matrix" value="BLOSUM62"/> |
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221 <param name="comp-based-stat" value="1"/> |
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222 <param name="masking" value="1"/> |
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223 <conditional name="hit_filter"> |
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224 <param name="hit_filter_select" value="max"/> |
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225 <param name="max_target_seqs" value="25" /> |
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226 </conditional> |
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227 <conditional name="filter_score"> |
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228 <param name="filter_score_select" value="evalue"/> |
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229 <param name="evalue" value="0.001" /> |
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230 </conditional> |
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231 <param name="id" value="0"/> |
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232 <param name="query_cover" value="0"/> |
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233 <param name="block_size" value="2"/> |
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234 <output name="blast_tabular" file="diamond_results.tabular"/> |
0 | 235 </test> |
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236 <test> |
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237 <conditional name="method_cond"> |
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238 <param name="method_select" value="blastp" /> |
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239 </conditional> |
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240 <param name="query" value="protein.fasta" ftype="fasta"/> |
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241 <conditional name="ref_db_source"> |
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242 <param name="db_source" value="history"/> |
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243 <param name="reference_database" value="db-wtax.dmnd"/> |
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244 </conditional> |
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245 <conditional name="tax_cond"> |
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246 <param name="tax_select" value="list"/> |
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247 <param name="taxonlist" value="2" /> |
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248 </conditional> |
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249 <conditional name="output"> |
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250 <param name="outfmt" value="6"/> |
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251 <param name="fields" value="qseqid,sseqid,pident,length,mismatch,gapopen,qstart,qend,sstart,send,evalue,bitscore"/> |
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252 </conditional> |
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253 <param name="sensitivity" value="0"/> |
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254 <param name="matrix" value="BLOSUM62"/> |
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255 <param name="comp-based-stat" value="1"/> |
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256 <param name="masking" value="1"/> |
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257 <conditional name="hit_filter"> |
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258 <param name="hit_filter_select" value="max"/> |
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259 <param name="max_target_seqs" value="25" /> |
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260 </conditional> |
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261 <conditional name="filter_score"> |
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262 <param name="filter_score_select" value="evalue"/> |
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263 <param name="evalue" value="0.001" /> |
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264 </conditional> |
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265 <param name="id" value="0"/> |
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266 <param name="query_cover" value="0"/> |
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267 <param name="block_size" value="2"/> |
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268 <output name="blast_tabular" file="diamond_results.wtax.tabular"/> |
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269 </test> |
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270 <test> |
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271 <conditional name="method_cond"> |
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272 <param name="method_select" value="blastx" /> |
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273 <conditional name="frameshift_cond"> |
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274 <param name="frameshift_select" value="yes"/> |
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275 </conditional> |
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276 </conditional> |
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277 <param name="query" value="nucleotide.fasta" ftype="fasta"/> |
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278 <conditional name="ref_db_source"> |
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279 <param name="db_source" value="history"/> |
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280 <param name="reference_database" value="db.dmnd"/> |
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281 </conditional> |
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282 <conditional name="output"> |
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283 <param name="outfmt" value="0"/> |
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284 </conditional> |
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285 <param name="sensitivity" value="0"/> |
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286 <param name="matrix" value="BLOSUM62"/> |
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287 <param name="comp-based-stat" value="1"/> |
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288 <param name="masking" value="1"/> |
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289 <conditional name="hit_filter"> |
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290 <param name="hit_filter_select" value="top"/> |
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291 <param name="top" value="10" /> |
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292 </conditional> |
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293 <conditional name="filter_score"> |
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294 <param name="filter_score_select" value="score"/> |
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295 <param name="evalue" value="1" /> |
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296 </conditional> |
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297 <param name="id" value="0"/> |
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298 <param name="query_cover" value="0"/> |
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299 <param name="block_size" value="2"/> |
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300 <output name="blast_tabular" file="diamond_results.pairwise"/> |
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301 </test> |
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302 <test> |
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303 <conditional name="method_cond"> |
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304 <param name="method_select" value="blastp" /> |
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305 </conditional> |
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306 <param name="query" value="protein.fasta" ftype="fasta"/> |
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307 <conditional name="ref_db_source"> |
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308 <param name="db_source" value="history"/> |
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309 <param name="reference_database" value="db-wtax.dmnd"/> |
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310 </conditional> |
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311 <conditional name="output"> |
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312 <param name="outfmt" value="100"/> |
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313 </conditional> |
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314 <output name="daa_output" file="diamond_results.daa" compare="sim_size" delta="10"/> |
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315 </test> |
0 | 316 </tests> |
317 <help> | |
318 <![CDATA[ | |
319 | |
320 **What it does** | |
321 | |
322 DIAMOND_ is a new alignment tool for aligning short DNA sequencing reads to a protein reference database such as NCBI-NR. | |
323 On Illumina reads of length 100-150bp, in fast mode, DIAMOND is about 20,000 times faster than BLASTX, while reporting | |
324 about 80-90% of all matches that BLASTX finds, with an e-value of at most 1e-5. In sensitive mode, DIAMOND ist about 2,500 | |
325 times faster than BLASTX, finding more than 94% of all matches. | |
326 | |
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327 The DIAMOND algorithm is designed for the alignment of large datasets. The algorithm is not efficient for a small number of query sequences or only a single one of them, and speed will be low. BLAST is recommended for small datasets. |
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328 |
0 | 329 .. _DIAMOND: http://ab.inf.uni-tuebingen.de/software/diamond/ |
330 | |
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331 **Input** |
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332 |
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333 Input data is a large protein or nucleotide sequence file. |
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334 |
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335 |
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336 **Output** |
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337 |
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338 Diamond gives you a tabular output file with 12 columns: |
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339 |
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340 Column Description |
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341 1 Query Seq-id (ID of your sequence) |
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342 2 Subject Seq-id (ID of the database hit) |
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343 3 Percentage of identical matches |
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344 4 Alignment length |
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345 5 Number of mismatches |
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346 6 Number of gap openings |
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347 7 Start of alignment in query |
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348 8 End of alignment in query |
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349 9 Start of alignment in subject (database hit) |
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350 10 End of alignment in subject (database hit) |
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351 11 Expectation value (E-value) |
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352 12 Bit score |
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353 |
0 | 354 |
355 Supported values for gap open and gap extend parameters depending on the selected scoring matrix. | |
356 | |
357 ======== ============================================ | |
358 Matrix Supported values for (gap open)/(gap extend) | |
359 ======== ============================================ | |
360 BLOSUM45 (10-13)/3; (12-16)/2; (16-19)/1 | |
361 BLOSUM50 (9-13)/3; (12-16)/2; (15-19)/1 | |
362 BLOSUM62 (6-11)/2; (9-13)/1 | |
363 BLOSUM80 (6-9)/2; 13/2; 25/2; (9-11)/1 | |
364 BLOSUM90 (6-9)/2; (9-11)/1 | |
365 PAM250 (11-15)/3; (13-17)/2; (17-21)/1 | |
366 PAM70 (6-8)/2; (9-11)/1 | |
367 PAM30 (5-7)/2; (8-10)/1 | |
368 ======== ============================================ | |
369 | |
370 | |
371 ]]> | |
372 </help> | |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/diamond commit cc80b878817d052398db16574917900ebe15292e
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373 <expand macro="citations" /> |
0 | 374 </tool> |