Mercurial > repos > bgruening > diffbind
diff diffbind.R @ 13:1de83981d43c draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/diffbind commit 13485bed6a57ec4a34cab4ec6bb8b36d219e3610
author | iuc |
---|---|
date | Wed, 30 May 2018 12:25:42 -0400 |
parents | 4c7ab9995f9e |
children | 194e3f2c1d86 |
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--- a/diffbind.R Thu Apr 19 17:15:53 2018 -0400 +++ b/diffbind.R Wed May 30 12:25:42 2018 -0400 @@ -94,12 +94,49 @@ # Output differential binding sites resSorted <- diff_bind[order(diff_bind$FDR),] -write.table(as.data.frame(resSorted), file = opt$outfile, sep="\t", quote = FALSE, append=TRUE, row.names = FALSE) +# Convert from GRanges (1-based) to 0-based format (adapted from https://www.biostars.org/p/89341/) +if (opt$format == "bed") { + resSorted <- data.frame(Chrom=seqnames(resSorted), + Start=start(resSorted) - 1, + End=end(resSorted), + Name=rep("DiffBind", length(resSorted)), + Score=rep("0", length(resSorted)), + Strand=gsub("\\*", ".", strand(resSorted))) +} else if (opt$format == "interval") { + # Output as interval + df <- as.data.frame(resSorted) + extrainfo <- NULL + for (i in 1:nrow(df)) { + extrainfo[i] <- paste0(c(df$width[i], df[i, 6:ncol(df)]), collapse="|") + } + resSorted <- data.frame(Chrom=seqnames(resSorted), + Start=start(resSorted) - 1, + End=end(resSorted), + Name=rep("DiffBind", length(resSorted)), + Score=rep("0", length(resSorted)), + Strand=gsub("\\*", ".", strand(resSorted)), + Comment=extrainfo) +} else { + # Output as 0-based tabular + resSorted <- data.frame(Chrom=seqnames(resSorted), + Start=start(resSorted) - 1, + End=end(resSorted), + Name=rep("DiffBind", length(resSorted)), + Score=rep("0", length(resSorted)), + Strand=gsub("\\*", ".", strand(resSorted)), + mcols(resSorted)) +} +write.table(resSorted, file = opt$outfile, sep="\t", quote = FALSE, append=TRUE, row.names = FALSE) # Output binding affinity scores if (!is.null(opt$bmatrix)) { bmat <- dba.peakset(sample_count, bRetrieve=TRUE, DataType=DBA_DATA_FRAME) - write.table(as.data.frame(bmat), file="bmatrix.tab", sep="\t", quote=FALSE, row.names=FALSE) + # Output as 0-based tabular + bmat <- data.frame(Chrom=bmat[, 1], + Start=bmat[, 2] - 1, + End=bmat[, 3], + bmat[, 4:ncol(bmat)]) + write.table(bmat, file="bmatrix.tab", sep="\t", quote=FALSE, row.names=FALSE) } # Output RData file