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annotate flye.xml @ 0:d9f4c141d88a draft

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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/flye commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
author bgruening
date Tue, 25 Sep 2018 05:24:27 -0400
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children cd256484eb1a
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0
d9f4c141d88a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/flye commit 2f6d48e1d2161d03411d9fbb4fc3d16f0fa3d2e1
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1 <tool id="flye" name="Assembly" version="2.3.5">
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2 <description>of long and error-prone reads</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <version_command>flye --version</version_command>
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8 <command detect_errors="exit_code">
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9 <![CDATA[
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10
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11 #for $counter, $input in enumerate($inputs):
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12 ln -s '$input' ./input_${counter}.${input.ext} &&
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13 #end for
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14
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15 flye
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16 $mode
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17 #for $counter, $input in enumerate($inputs):
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18 ./input_${counter}.${input.ext}
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19 #end for
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20
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21 -o out_dir
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22 -g '$g'
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23 -t \${GALAXY_SLOTS:-4}
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24 -i $i
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25 #if $m:
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26 -m '$m'
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27 #end if
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28 2>&1
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29 ]]></command>
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30 <inputs>
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31 <param name="inputs" type="data" format="fasta,fasta.gz,fastq,fastq.gz" multiple="true" label="Input reads" />
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32 <param name="mode" type="select" label="Mode">
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33 <option value="--nano-raw">Nanopore raw</option>
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34 <option value="--nano-corr">Nanopore corrected</option>
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35 <option value="--pacbio-raw">PacBio raw</option>
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36 <option value="--pacbio-corr">PacBio corrected</option>
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37 <option value="--subassemblies">high-quality contig-like input</option>
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38 </param>
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39 <param argument="-g" type="text" label="estimated genome size (for example, 5m or 2.6g)">
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40 <validator type="regex" message="Genome size must be a float or integer, optionally followed by the a unit prefix (kmg)">^([0-9]*[.])?[0-9]+[kmg]?$</validator>
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41 </param>
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42 <param argument="-i" type="integer" value="1" label="number of polishing iterations" />
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43 <param argument="-m" type="integer" optional="true" label="minimum overlap between reads (default: auto)" />
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44 </inputs>
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45 <outputs>
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46 <data name="contigs" format="fasta" from_work_dir="out_dir/contigs.fasta" label="${tool.name} on ${on_string} (contigs)"/>
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47 <data name="scaffolds" format="fasta" from_work_dir="out_dir/scaffolds.fasta" label="${tool.name} on ${on_string} (scaffolds)"/>
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48 <data name="assembly_info" format="tabular" from_work_dir="out_dir/assembly_info.txt" label="${tool.name} on ${on_string} (assembly_info)"/>
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49 <data name="assembly_graph" format="graph_dot" from_work_dir="out_dir/assembly_graph.dot" label="${tool.name} on ${on_string} (assembly_graph)"/>
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50 <data name="flye_log" format="txt" from_work_dir="out_dir/flye.log" label="${tool.name} on ${on_string} (log)"/>
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51 </outputs>
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52 <tests>
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53 <test>
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54 <param name="inputs" ftype="fasta" value="E.coli_PacBio_40x_first_200_reads.fasta"/>
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55 <param name="mode" value="--pacbio-raw"/>
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56 <param name="g" value="1m"/>
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57 <output name="contigs" file="result1_contigs.fasta" ftype="fasta"/>
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58 <output name="scaffolds" file="result1_scaffolds.fasta" ftype="fasta"/>
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59 <output name="assembly_info" file="result1_assembly_info.txt" ftype="tabular"/>
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60 <output name="assembly_graph" file="result1_assembly_graph.dot" ftype="graph_dot" compare="sim_size"/>
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61 </test>
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62 <test>
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63 <param name="inputs" ftype="fasta" value="Loman_E.coli_MAP006-1_2D_50x_first_500_reads.fasta"/>
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64 <param name="mode" value="--nano-raw"/>
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65 <param name="g" value="100000"/>
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66 <output name="contigs" file="result2_contigs.fasta" ftype="fasta"/>
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67 <output name="scaffolds" file="result2_scaffolds.fasta" ftype="fasta"/>
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68 <output name="assembly_info" file="result2_assembly_info.txt" ftype="tabular"/>
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69 <output name="assembly_graph" file="result2_assembly_graph.dot" ftype="graph_dot" compare="sim_size"/>
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70 </test>
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71 <test>
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72 <param name="inputs" ftype="fasta" value="E.coli_PacBio_40x_first_200_reads.fasta"/>
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73 <param name="mode" value="--pacbio-raw"/>
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74 <param name="g" value="1.1m"/>
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75 <param name="i" value="2"/>
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76 <output name="contigs" file="result3_contigs.fasta" ftype="fasta"/>
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77 <output name="scaffolds" file="result3_scaffolds.fasta" ftype="fasta"/>
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78 </test>
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79 </tests>
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80 <help><![CDATA[
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81
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82 Input reads could be in FASTA or FASTQ format, uncompressed
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83 or compressed with gz. Currenlty, raw and corrected reads
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84 from PacBio and ONT are supported. The expected error rates are
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85 <30% for raw and <2% for corrected reads. Additionally,
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86 --subassemblies option performs a consensus assembly of multiple
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87 sets of high-quality contigs. You may specify multiple
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88 files with reads (separated by spaces). Mixing different read
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89 types is not yet supported.
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90
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91 You must provide an estimate of the genome size as input,
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92 which is used for solid k-mers selection. The estimate could
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93 be rough (e.g. withing 0.5x-2x range) and does not affect
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94 the other assembly stages. Standard size modificators are
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95 supported (e.g. 5m or 2.6g).
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96
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97 ]]></help>
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98 <expand macro="citations" />
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99 </tool>