Mercurial > repos > bgruening > glimmer_hmm

annotate glimmerHMM/glimmerhmm_tabular_to_sequence.py @ 1:4da91bb244dc draft

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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 2effed877a778e455c63a76e994a0f2bb8f4dba0
author rmarenco
date Thu, 14 Jul 2016 15:11:33 -0400
parents c9699375fcf6
children
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c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
bgruening
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1 #!/usr/bin/env python
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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2 """Convert GlimmerHMM gene predictions into protein sequences.
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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4 This works with the GlimmerHMM specific output format:
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6 12 1 + Initial 10748 10762 15
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9 12 4 + Internal 11072 11110 39
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10 12 5 + Internal 11146 11221 76
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11 12 6 + Terminal 11265 11388 124
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c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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13 http://www.cbcb.umd.edu/software/GlimmerHMM/
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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15 Modified version of the converter from Brad Chapman: https://github.com/chapmanb/bcbb/blob/master/biopython/glimmer_to_proteins.py
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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17 Usage:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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18 glimmer_to_proteins.py <glimmer output> <ref fasta> <output file> <convert to protein ... False|True>
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19 """
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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21 import sys
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22 import os
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23 import operator
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c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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25 from Bio import SeqIO
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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26 from Bio.SeqRecord import SeqRecord
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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28 def main(glimmer_file, ref_file, out_file, to_protein):
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29 with open(ref_file) as in_handle:
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30 ref_rec = SeqIO.read(in_handle, "fasta")
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32 base, ext = os.path.splitext(glimmer_file)
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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34 with open(out_file, "w") as out_handle:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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35 SeqIO.write(protein_recs(glimmer_file, ref_rec, to_protein), out_handle, "fasta")
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37 def protein_recs(glimmer_file, ref_rec, to_protein):
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38 """Generate protein records
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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39 """
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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40 with open(glimmer_file) as in_handle:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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41 for gene_num, exons, strand in glimmer_predictions(in_handle):
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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42 seq_exons = []
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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43 for start, end in exons:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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44 seq_exons.append(ref_rec.seq[start:end])
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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45 gene_seq = reduce(operator.add, seq_exons)
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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46 if strand == '-':
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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47 gene_seq = gene_seq.reverse_complement()
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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48 if to_protein:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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49 yield SeqRecord(gene_seq.translate(), gene_num, "", "")
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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50 else:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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51 yield SeqRecord(gene_seq, gene_num, "", "")
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52
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53 def glimmer_predictions(in_handle):
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54 """Parse Glimmer output, generating a exons and strand for each prediction.
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55 """
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56 # read the header
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57 while 1:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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58 line = in_handle.readline()
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59 if line.startswith(" # #"):
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60 break
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61 in_handle.readline()
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62 # read gene predictions one at a time
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63 cur_exons, cur_gene_num, cur_strand = ([], None, None)
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64 while 1:
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65 line = in_handle.readline()
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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66 if not line:
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67 break
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68 parts = line.strip().split()
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69 # new exon
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70 if len(parts) == 0:
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71 yield cur_gene_num, cur_exons, cur_strand
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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72 cur_exons, cur_gene_num, cur_strand = ([], None, None)
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73 else:
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74 this_gene_num = parts[0]
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75 this_strand = parts[2]
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76 this_start = int(parts[4]) - 1 # 1 based
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77 this_end = int(parts[5])
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78 if cur_gene_num is None:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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79 cur_gene_num = this_gene_num
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80 cur_strand = this_strand
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81 else:
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
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82 assert cur_gene_num == this_gene_num
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83 assert cur_strand == this_strand
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84 cur_exons.append((this_start, this_end))
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85 if len(cur_exons) > 0:
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86 yield cur_gene_num, cur_exons, cur_strand
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87
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88 if __name__ == "__main__":
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89 if len(sys.argv) != 5:
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bgruening
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diff changeset
90 print __doc__
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
bgruening
parents:
diff changeset
91 sys.exit()
c9699375fcf6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
bgruening
parents:
diff changeset
92 main(*sys.argv[1:])