Mercurial > repos > bgruening > glimmer_hmm
diff readme.md @ 0:c9699375fcf6 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/glimmer_hmm commit 0dc67759bcbdf5a8a285ded9ba751340d741fe63
author | bgruening |
---|---|
date | Wed, 13 Jul 2016 10:55:48 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/readme.md Wed Jul 13 10:55:48 2016 -0400 @@ -0,0 +1,82 @@ +Galaxy wrapper for GlimmerHMM +===================================== + +This wrapper has been written in 2012 by Björn Grüning, and updated by Rémi Marenco in 2016. + +This is a wrapper for the command line tool of GlimmerHMM. +https://ccb.jhu.edu/software/glimmerhmm/ + +GlimmerHMM is a gene finder based on a Generalized Hidden Markov Model (GHMM). Although the gene finder conforms to the overall mathematical framework of a GHMM, +additionally it incorporates splice site models adapted from the GeneSplicer program and a decision tree adapted from GlimmerM. It also utilizes +Interpolated Markov Models for the coding and noncoding models. +Currently, GlimmerHMM's GHMM structure includes introns of each phase, intergenic regions, and four types of exons (initial, internal, final, and single). + +Majoros, W.H., Pertea, M., and Salzberg, S.L. TigrScan and GlimmerHMM: two open-source ab initio eukaryotic gene-finders Bioinformatics 20 2878-2879. +Pertea, M. and S. L. Salzberg (2002). "Computational gene finding in plants." Plant Molecular Biology 48(1-2): 39-48. +The Arabidopsis Genome Initiative, (2000) "Analysis of the genome sequence of the flowering plant Arabidopsis thaliana", Nature. Dec 14; 408(6814):796-815. +Pertea, M., S. L. Salzberg, et al. (2000). "Finding genes in Plasmodium falciparum." Nature 404(6773): 34; discussion 34-5. +Salzberg, S. L., M. Pertea, et al. (1999). "Interpolated Markov models for eukaryotic gene finding." Genomics 59(1): 24-31. + + +Installation +============ + +To install Glimmer3, please download GlimmerHMM from + +ftp://ccb.jhu.edu/pub/software/glimmerhmm + +and follow the installation instructions. +To extract the glimmerHMM predicted genes, the GFF Parser from Brad Chapman (ttp://github.com/chapmanb/bcbb/tree/master/gff) was used and is included. + +To install the wrapper copy the glimmerHMM folder in the galaxy tools +folder and modify the $GALAXY_ROOT/config/tool_conf.xml file to make the tool available to Galaxy. +For example: + +```xml +<tool file="gene_prediction/tools/glimmerHMM/glimmerhmm_predict.xml" /> +<tool file="gene_prediction/tools/glimmerHMM/glimmerhmm_to_sequence.xml" /> +``` + +You also need to use a trained organism by adding them as reference data in Galaxy: + +1. Add the *glimmer_hmm_trained_dir* data table to `tool_data_table_conf.xml` in `$GALAXY_ROOT/config/`: + + ```xml + <!-- glimmer_hmm trained_dir --> + <table name="glimmer_hmm_trained_dir" comment_char="#"> + <columns>value, name, path</columns> + <file path="tool-data/glimmer_hmm.loc" /> + </table> + ``` + +2. Add the `glimmer_hmm.loc` file referencing your trained organism, in `tool-data`. + You have a sample [`glimmer_hmm.loc.sample`] available in the repository to help you configuring it properly +3. Add your data in the chosen folder at step 2. You can get them from the GlimmerHMM tar, `$GLIMMERHMM/trained_dir` + +History +======= + +- v2.0 - Update by Rémi Marenco to make it work without having to modify the wrapper + add ability to select the species +- v0.1 - Initial public release + + +Wrapper Licence (MIT/BSD style) +=============================== + +Permission to use, copy, modify, and distribute this software and its +documentation with or without modifications and for any purpose and +without fee is hereby granted, provided that any copyright notices +appear in all copies and that both those copyright notices and this +permission notice appear in supporting documentation, and that the +names of the contributors or copyright holders not be used in +advertising or publicity pertaining to distribution of the software +without specific prior permission. + +THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL +WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED +WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE +CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT +OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS +OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE +OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE +OR PERFORMANCE OF THIS SOFTWARE.