Mercurial > repos > bgruening > hicexplorer_hicaggregatecontacts

annotate hicAggregateContacts.xml @ 15:63c8377c58b3 draft default tip

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 79133bc3739fbcc6c2055d589679aae312161d03
author iuc
date Tue, 05 Nov 2024 00:10:46 +0000
parents f0216498a666
children
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1 <tool id="hicexplorer_hicaggregatecontacts" name="@BINARY@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>allow plotting of aggregated Hi-C contacts between regions specified in a file</description>
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3 <macros>
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4 <token name="@BINARY@">hicAggregateContacts</token>
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5 <import>macros.xml</import>
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6 </macros>
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7 <expand macro="requirements" />
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8 <command detect_errors="exit_code"><![CDATA[
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9 ln -s '$matrix_h5_cooler' 'matrix.$matrix_h5_cooler.ext' &&
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10 @BINARY@
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11 --matrix 'matrix.$matrix_h5_cooler.ext'
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12 --BED '$BED'
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13 #if $BED2:
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14 --BED2 '$BED2'
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15 #end if
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16
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17 --range '$range_min:$range_max'
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18 --numberOfBins '$numberOfBins'
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20 --transform $transform
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21 --operationType $operationType
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22 --mode $mode
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23 $row_wise
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24 $perChr
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25 --largeRegionsOperation $largeRegionsOperation
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26 #if $outputs and 'PrefixMatrix' in $outputs:
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27 --outFilePrefixMatrix 'matrix_values'
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28 #end if
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29
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30 #if $outputs and 'outFileContactPairs' in $outputs:
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31 --outFileContactPairs 'contact_positions'
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32 #end if
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34 #if $outputs and 'HeatmapFile' in $outputs:
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35 --diagnosticHeatmapFile 'heatmap'
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36 #end if
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37
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38 #if $outputs and 'outFileObsExp' in $outputs:
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39 --outFileObsExp 'ObsExp.$matrix_h5_cooler.ext'
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40 #end if
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41
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42 #if $clustering:
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43 $clustering
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44 #end if
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46 #if $howToCluster:
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47 --howToCluster $howToCluster
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48 #end if
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50 #if $chromosomes:
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51 --chromosomes #echo "' '".join([ "'%s'" % $chrom.chromosome for $chrom in $chromosomes ])#
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52 #end if
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54 #if $plotType:
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55 --plotType $plotType
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56 #end if
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58 #if $colormap:
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59 --colorMap $colormap
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60 #end if
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ccd1d3013827 planemo upload for repository https://github.com/maxplanck-ie/HiCExplorer/tree/master/galaxy/wrapper/ commit 0456f085bac2c88b8cbddfcf12b02776d2a0d457
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62 #if $vMin:
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63 --vMin $vMin
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64 #end if
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66 #if $vMax:
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67 --vMax $vMax
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68 #end if
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70 $considerStrandDirection
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71
0
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72 --outFileName plot.$image_file_format
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73 &&
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74 mv plot.$image_file_format plot
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75 #if $outputs and 'outFileObsExp' in $outputs:
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76 && mv 'ObsExp.$matrix_h5_cooler.ext' matrix
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77 #end if
0
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79 ]]>
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80 </command>
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81 <inputs>
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82 <expand macro="matrix_h5_cooler_macro" />
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83 <param argument="--BED" type="data" format="bed" label="Interactions between regions in this BED file are plotted" />
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84 <param argument="--BED2" type="data" format="bed" optional="true" label="Interactions between regions in first and second BED file are plotted" />
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85
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86 <expand macro="range" />
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87 <param argument="--mode" type="select" label="Regions to consider.">
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88 <option value="inter-chr">inter-chr</option>
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89 <option value="intra-chr">intra-chr</option>
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90 <option value="all" selected="true">all</option>
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91 </param>
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92
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93 <param argument="--row_wise" type="boolean" truevalue="--row_wise" falsevalue="" label="Row wise" help="If given,the insteractions between each row of the BED file and its
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94 corresponding row of the BED2 file are computed. If intra-chromosomal
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95 contacts are computed, the rows with different chromosomes are ignored.
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96 If inter-chromosomal, the rows with same chromosomes are ignored.
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97 It keeps all the rows if `all`." />
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98
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99 <param argument="--perChr" type="boolean" truevalue="--perChr" falsevalue="" label="Per chromosome" help="If set, it generates a plot per chromosome. It is only affected if intra-chromosomal contacts are of interest." />
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100
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101 <repeat name="chromosomes" title="List of chromosomes to plot" min="0">
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102 <param name="chromosome" type="text" label="chromosome (one per field)">
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103 <validator type="empty_field" />
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104 </param>
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105 </repeat>
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106
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107 <param argument="--numberOfBins" type="integer" optional="true" label="Number of bins to include in the submatrix" help="The bed regions will be centered between -half number of bins and +half number of bins indicated." />
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108
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109 <param argument="--transform" type="select" label="Type of transformation for the matrix" help="If total counts are selected, then the sub-matrix values
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110 are divided by the total counts for normalization. If
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111 z-score or obs/exp are selected, then H-C matrix is
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112 converted into a z-score or observed / expected matrix.">
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113 <option value="none" selected="true">none</option>
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114 <option value="total-counts">total-counts</option>
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115 <option value="z-score">z-score</option>
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116 <option value="obs/exp">obs/exp</option>
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117 </param>
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118
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119 <param argument="--operationType" type="select" label="Type of operation to compute final matrix">
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120 <option value="median" selected="true">median</option>
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121 <option value="mean">mean</option>
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122 <option value="sum">sum</option>
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123 </param>
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124 <param argument="--considerStrandDirection" type="boolean" truevalue="--considerStrandDirection" falsevalue="" label="Consider strand direction" help="This parameter specifies if the strand information is taken into account for the aggregation. It has the effect that the contacts of a reverse strand region are inverted e.g. [1,2,3] becomes [3,2,1]." />
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125
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126 <param argument="--largeRegionsOperation" type="select" label="Large regions operation" help="If a given coordinate in the bed file is larger than
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127 a bin of the input matrix, by default only the first bin
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128 is taken into account. However there are more posibilities
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129 to handel such a case. Users can ask for the last bin or
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130 for center of the region. As an example if a region falls into bins [4,5,6]
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131 and `--numberOfBins = 2` then if first, bins [3,4,5] are kept.
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132 If last: [5,6,7] and if center: [4,5,6].">
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133 <option value="first" selected="true">first</option>
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134 <option value="last">last</option>
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135 <option value="center">center</option>
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136 </param>
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137
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138 <param name="clustering" type="select" optional="true" label="Number of clusters per chromosome" help="When this option is set, then the matrix is split into
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139 clusters using the hierarchical clustering algorithm,
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140 using 'ward linkage'. hclust could be very slow if
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141 you have >1000 submatrices per chromosome. In those
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142 cases, you might prefer kmeans.">
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143 <option value="--kmeans">kmeans</option>
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144 <option value="--hclust">hclust (#clusters per chromosome)</option>
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145 <option value="--spectral">spectral</option>
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146 </param>
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147
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148 <param argument="--howToCluster" type="select" optional="true" label="How to cluster" help="Options are 'full', 'center' and 'diagonal'. The full clustering '
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149 'takes all values of each submatrix for clustering. center, takes only a square of '
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150 'length 3x3 from each submatrix and uses only this values for clustering. With the '
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151 'diagonal option the clustering is only carried out based on the submatrix diagonal '
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152 '(representing values at the same distance to each other)">
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153 <option value="full">full</option>
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154 <option value="center">center</option>
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155 <option value="diagonal">diagonal</option>
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156 </param>
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157 <param argument="--plotType" type="select" optional="true" label="Plot type">
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158 <option value="2d">2D</option>
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159 <option value="3d">3D</option>
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160 </param>
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161 <expand macro="colormap" />
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162 <param argument="--vMin" type="float" optional="true" label="vMin" help= "Minimum value of the plotted score." />
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163 <param argument="--vMax" type="float" optional="true" label="vMax" help= "Maximum value of the plotted score." />
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164 <param name="image_file_format" type="select" label="Image output format">
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165 <option value="png" selected="True">png</option>
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166 <option value="svg">svg</option>
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167 <option value="pdf">pdf</option>
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168 </param>
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169
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170 <param name="outputs" type="select" optional="true" multiple="true" label="Optional output files">
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171 <option value="PrefixMatrix">Save values underlying the final matrix</option>
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172 <option value="outFileContactPairs">Save the position of the contacts</option>
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173 <option value="HeatmapFile">Heatmap file per chromosome</option>
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174 <option value="outFileObsExp">Save obs/exp matrix</option>
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175 </param>
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176
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177 </inputs>
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178 <outputs>
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179 <data name="outFileName" from_work_dir="plot" format="png" label="${tool.name} on ${on_string}: Plot">
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180 <change_format>
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181 <when input="image_file_format" value="svg" format="svg" />
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182 <when input="image_file_format" value="pdf" format="pdf" />
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183 </change_format>
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184 </data>
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185 <collection name="matrix_values" type="list" label="${tool.name} on ${on_string}: Matrix values">
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186 <discover_datasets pattern="matrix_values_(?P&lt;designation&gt;.*)\..*" directory="./" format="tabular" />
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187 </collection>
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188 <collection name="contact_positions" type="list" label="${tool.name} on ${on_string}: Contact positions">
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189 <discover_datasets pattern="contact_positions_(?P&lt;designation&gt;.*)\..*" directory="./" format="tabular" />
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190 </collection>
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191 <collection name="heatmap" type="list" label="${tool.name} on ${on_string}: Heatmap">
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192 <discover_datasets pattern="heatmap_(?P&lt;designation&gt;.*)\..*" directory="./" format="tabular" />
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193 </collection>
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194 <data name="matrix_obs_exp_output" from_work_dir="matrix" format="h5" label="${tool.name} MATRIX on ${on_string}">
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195 <filter>outputs == 'outFileObsExp'</filter>
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196 <change_format>
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197 <when input="matrix_h5_cooler.ext" value="cool" format="cool" />
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198 </change_format>
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199 </data>
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200 </outputs>
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201 <tests>
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202 <test expect_num_outputs="4">
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203 <param name="matrix_h5_cooler" value="Li_et_al_2015.cool" ftype="cool" />
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204 <param name="BED" value="hicAggregateContacts/test_regions.bed" ftype="bed" />
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205 <param name="numberOfBins" value="30" />
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206 <param name="range_max" value="900000" />
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207 <param name="range_min" value="50000" />
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208 <param name="howToCluster" value="center" />
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209 <param name="image_file_format" value="png" />
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210 <output name="outFileName" value="hicAggregateContacts_results1.png" compare="sim_size" />
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211 </test>
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212 </tests>
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213 <help><![CDATA[
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214
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215 Aggregation of Hi-C contacts
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216 ============================
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217
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218 **hicAggregateContacts** allows plotting of aggregated Hi-C sub-matrices of a specified list of positions. Positions of interest can for example be binding sites of a specific protein that were determined by ChIP-seq or genetic elements as transcription start sites of active genes.
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219
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220 _________________
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221
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222 Usage
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223 -----
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224
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225 This tool must be used on Hi-C matrices corrected by ``hicCorrectMatrix``. One should also consider bigger bins than restriction enzyme resolution bins using ``hicMergeMatrixBins``.
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226
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227 _________________
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228
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229 Optional parameters
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230 -------------------
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231
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232 Optional data output can be selected:
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233
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234 - **Save values underlying the final matrix:** if this option is given, then the values underlying the final matrix will be saved to tab-delimited tables (one per chromosome) using the indicated prefix, for example TSS_to_TSS_chrX.tab. If clustering is performed, then the values are saved including the cluster_id a in TSS_to_TSS_chrX_cluster_1.tab
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235
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236 - **Save the position of the contacts:** if this option is given, then the position of the contacts is saved as (chrom1, start1, end1, chrom2, start2, end2) where chrom_n, start_n, end_n correspond to the pair of positions used to compute the submatrix. The data is saved per chromosome and per cluster separately (one file each).
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237
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238 - **Heatmap file per chromosome:** if given, a heatmap file (per chromosome) is saved. Each row in the heatmap contains the diagonal of each of the submatrices centered on the bed file. This file is useful to get an idea of the values that are used for the aggregate matrix and to determine the fraction of submatrices that are aggregated that may have an enrichment at the center.
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239
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240 _________________
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241
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242 Output
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243 ------
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244
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245 **hicAggregateContacts** outputs a plot of aggregated contacts.
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246
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247 Below, you can find an example of an aggregate Hi-C matrix obtained from *Drosophila melanogaster* Hi-C data. The interactions are plotted at binding sites of a protein that were determined by ChIP-seq. We plot sub-matrices of 30 bins (1.5 kb bin size, 45 kb in total). The regions specified in the BED file will be centered between half number of bins and the other half number of bins.The considered range is 300-1000 kb. The range should be adjusted and only contain contacts larger than TAD size to reduce background interactions.
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248
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249 .. image:: $PATH_TO_IMAGES/hicAggregateContacts.png
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250 :width: 80 %
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252 This example was calculated using mean interactions of an observed vs. expected transformed Hi-C matrix. Additional options for the matrix transformation are total-counts or z-score. Aggregate contacts can be plotted in 2D or 3D.
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254 _________________
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256 | For more information about HiCExplorer please consider our documentation on readthedocs.io_
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258 .. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html
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259 .. _Colormaps: https://matplotlib.org/examples/color/colormaps_reference.html
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ee370ade24cd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 2a0943e78bdc8ebb13f181399206a9eea37ed78f"
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260 ]]> </help>
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261 <expand macro="citations" />
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262 </tool>