Mercurial > repos > bgruening > hicexplorer_hicdetectloops

annotate hicDetectLoops.xml @ 2:ce8d154fc6b5 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 4b602d427e0fc0da5353a4510798349de98e4ae4"
author iuc
date Wed, 11 Mar 2020 17:08:20 -0400
parents 2d1f9759fa0e
children 1119cdd14ddb
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2d1f9759fa0e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 3b41d687ff30583540d055f6995de00530cca81d-dirty"
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1 <tool id="hicexplorer_hicdetectloops" name="@BINARY@" version="@WRAPPER_VERSION@.0">
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2 <description>searches for enriched regions</description>
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3 <macros>
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4 <token name="@BINARY@">hicDetectLoops</token>
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5 <import>macros.xml</import>
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6 </macros>
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7 <expand macro="requirements" />
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8 <command detect_errors="exit_code"><![CDATA[
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9 ln -s '$matrix_h5_cooler' 'matrix.$matrix_h5_cooler.ext' &&
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10 @BINARY@
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11
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12 --matrix 'matrix.$matrix_h5_cooler.ext'
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2d1f9759fa0e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 3b41d687ff30583540d055f6995de00530cca81d-dirty"
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14 #if $peakWidth:
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15 --peakWidth $peakWidth
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16 #end if
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18 #if $windowSize:
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19 --windowSize $windowSize
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20 #end if
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22 #if $pValuePreselection:
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23 --pValuePreselection $pValuePreselection
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24 #end if
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26 #if $peakInteractionsThreshold:
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27 --peakInteractionsThreshold $peakInteractionsThreshold
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28 #end if
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30 #if $maximumInteractionPercentageThreshold:
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31 --maximumInteractionPercentageThreshold $maximumInteractionPercentageThreshold
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32 #end if
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33 #if $pValue:
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34 --pValue $pValue
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35 #end if
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36 #if $maxLoopDistance:
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37 --maxLoopDistance $maxLoopDistance
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38 #end if
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40 #if $region:
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41 --region '$region'
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42 #end if
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43
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44 #if $chromosomes:
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45 #set $chromosome = ' '.join([ '\'%s\'' % $chrom for $chrom in str($chromosomes).split(' ') ])
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46 --chromosomes $chromosome
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47 #end if
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48
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49 --statisticalTest $statisticalTest_selector
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50
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51 --outFileName output_loop.bedgraph
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52
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53 --threads @THREADS@ -tpc @THREADS@
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54 ]]>
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55 </command>
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56 <inputs>
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57 <expand macro="matrix_h5_cooler_macro" />
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58 <param argument="--peakWidth" type="integer" optional='true' label="Peak width" help= "The width of the peak region in bins. The square around the peak will include (2 * peakWidth)^2 bins." />
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59 <param argument="--windowSize" type="integer" optional='true' label="Window size" help= "The window size for the neighborhood region the peak is located in. All values from this region (exclude the values from the peak
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60 region) are tested against the peak region for significant difference. The square will have the size of (2 * windowSize)^2 bins" />
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61 <param argument="--pValuePreselection" type="float" label="P-value preselection" help= "Only candidates with p-values less the given threshold will be considered as candidates.
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62 For each genomic distance a negative binomial distribution is fitted and for each pixel a p-value given by the cumulative density function is given.
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63 This does NOT influence the p-value for the neighborhood testing." value='0.05'/>
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64 <param argument="--peakInteractionsThreshold" type="integer" label="Minimum interaction number" help= "The minimum number of interactions a detected peaks needs to have to be considered." value='5' />
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65 <param argument="--maximumInteractionPercentageThreshold" type="float" value='0.1' label="Maximum interaction share" help= "For each genomic distance the maximum value is considered and all candidates need to have at least \'max_value * maximumInteractionPercentageThreshold\' interactions." />
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66 <param argument="--pValue" type="float" label="P-value" help= "Rejection level for the statistical test for H0. H0 is peak region and background have the same distribution." value='0.05'/>
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67 <param argument="--maxLoopDistance" optional='true' type="integer" label="Maximal loop distance" help= "Maximum genomic distance of a loop, usually loops are within a distance of ~2MB." value='2000000'/>
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68 <param argument="--minLoopDistance" optional='true' type="integer" label="Maximal loop distance" help= "Minimum genomic distance of a loop to be considered." value='100000'/>
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69 <param argument="--chromosomes" optional='true' type="text" label="Chromosomes to include" help= "Chromosomes to include in the analysis. If not set, all chromosomes are included." />
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70 <param argument="--region" optional='true' type="text" label="Chromosomes to include" help= "The format is chr:start-end." />
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71 <param name="statisticalTest_selector" type="select" label="Stistical test">
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72 <option value="wilcoxon-rank-sum" selected="True">Wilcoxon rank-sum'</option>
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73 <option value="anderson-darling">Anderson-Darling</option>
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74 </param>
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75 </inputs>
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76 <outputs>
0
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77 <data name='output_loops' from_work_dir='output_loop.bedgraph' format='bedgraph' label='Computed loops'/>
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78 </outputs>
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79 <tests>
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80 <test>
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81 <param name="matrix_h5_cooler" value="small_test_matrix.cool"/>
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82 <param name="maxLoopDistance" value="30000000"/>
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83 <param name="windowSize" value="5"/>
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84 <param name="peakWidth" value="2"/>
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85 <param name="pValue" value="0.5"/>
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86 <param name="pValuePreselection" value="0.55"/>
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87 <!-- <param name="chromosomes" value="X"/> -->
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88 <output name="output_loops" file="hicDetectLoops/loops.bedgraph" ftype="bedgraph" compare="sim_size"/>
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89 </test>
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90 </tests>
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91 <help><![CDATA[
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92
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93 Loop detection
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94 ==============
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95
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96 Computes enriched regions (peaks) or long range contacts on the given contact matrix.
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97
2
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98 hicDetectLoops can detect enriched interaction regions (peaks / loops) based on a strict candidate selection, negative binomial distributions and Anderson-Darling / Wilcoxon rank-sum tests.
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99
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100 The algorithm was mainly develop on GM12878 cells from Rao 2014 on 10kb and 5kb fixed bin size resolution.
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101
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102 _________________
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103
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104 Usage
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105 -----
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106
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107 A command line example is available below (easily matchable in Galaxy using each field information):
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108
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109 ̀`$ hicDetectLoops -m matrix.cool -o loops.bedgraph --maxLoopDistance 2000000 --windowSize 10 --peakWidth 6 --pValuePreselection 0.05 --pValue 0.05 --peakInteractionsThreshold 20 --maximumInteractionPercentageThreshold 0.1 --statisticTest anderson-darling`
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110
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111 The candidate selection is based on the restriction of the maximum genomic distance, here 2MB. This distance is given by Rao 2014. For each genomic distance a negative binomial distribution is computed and only interaction pairs with a threshold less than ``--pValuePreselection`` are accepted. Detected candidates need to have at least an interaction count of ``--maximumInteractionPercentageThreshold`` times the maximum value for their genomic distance. Please note that ``--maximumInteractionPercentageThreshold`` was introduced with HiCExplorer release 3.2. Earlier versions did not have this parameter yet and therefore their outputs may differ. In a second step, each candidate is considered compared to its neighborhood. This neighborhood is defined by the ``--windowSize`` parameter in the x and y dimension. Per neighborhood only one candidate is considered, therefore only the candidate with the highest peak values is accepted. As a last step, the neighborhood is split into a peak and background region (parameter ``--peakWidth``). The peakWidth can never be larger than the windowSize. However, we recommend for 10kb matrices a windowSize of 10 and a peakWidth of 6.
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112
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113 The output file (´´-o loops.bedgraph``) contains the x and y position of each loop and its corresponding p-value of the Anderson-Darling test.
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114
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115 `1 120000000 122500000 1 145000000 147500000 0.001`
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116
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117 The results can visualized via hicPlotMatrix:
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118
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119 `$ hicPlotMatrix -m matrix.cool -o plot.png --log1p --region 1:18000000-22000000 --loops loops.bedgraph`
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120
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121 .. image:: $PATH_TO_IMAGES/hicDetectLoops.png
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122 :width: 50%
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123
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124
0
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125 For more information about HiCExplorer please consider our documentation on readthedocs.io_.
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126
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127 .. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html
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128
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129 ]]></help>
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130 <expand macro="citations" />
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131 </tool>