Mercurial > repos > bgruening > hicexplorer_hicfindtads

annotate hicFindTADs.xml @ 9:4ce06ae9893a draft

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planemo upload for repository https://github.com/maxplanck-ie/HiCExplorer/tree/master/galaxy/wrapper/ commit dddc0b9035b8edadfd45d74b01aeca245c2725d7
author iuc
date Fri, 27 Apr 2018 08:53:10 -0400
parents b05f292d220c
children 6b7987d22eab
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aab371aa615e planemo upload for repository https://github.com/maxplanck-ie/HiCExplorer/tree/master/galaxy/wrapper/ commit 2f347b0756d720114f037ed1ff9ba4836e1b3b04
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1 <tool id="hicexplorer_hicfindtads" name="@BINARY@" version="@WRAPPER_VERSION@.0">
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2 <description>identify TAD boundaries by computing the degree of separation of each Hi-C matrix bin</description>
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3 <macros>
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4 <token name="@BINARY@">hicFindTADs</token>
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5 <import>macros.xml</import>
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6 </macros>
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7 <expand macro="requirements" />
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8 <command detect_errors="exit_code"><![CDATA[
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9 hicFindTADs
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10 --matrix '$matrix_h5_cooler'
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12 --delta $delta
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14 #if $minBoundaryDistance:
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15 --minBoundaryDistance $minBoundaryDistance
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16 #end if
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17 --minDepth $minDepth
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18 --maxDepth $maxDepth
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19 --step $step
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20 #if $multiple_comparison_conditional.multiple_comparison_selector == 'fdr':
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21 --correctForMultipleTesting fdr
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22 --threshold $multiple_comparison_conditional.threshold
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23 #elif $multiple_comparison_conditional.multiple_comparison_selector == 'bonferroni':
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24 --correctForMultipleTesting bonferroni
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25 --threshold $multiple_comparison_conditional.threshold
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26 #else:
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27 --multipleComparisons None
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28 #end if
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29
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30 --numberOfProcessors @THREADS@
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31 --outPrefix galaxy_tad_prefix
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32 ]]></command>
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33 <inputs>
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34 <expand macro='matrix_h5_cooler_macro' />
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35
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36 <param argument="--minDepth" type="integer" value="40000"
a9c1d76b90c4 planemo upload for repository https://github.com/maxplanck-ie/HiCExplorer/tree/master/galaxy/wrapper/ commit 4d61b6bf2fed275ab38c226d0c4390b095a38251
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37 label="Minimum window length (in bp) to be considered to the left and to the right of each Hi-C bin."
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38 help="This number should be at least 3 times as large as the bin size of the Hi-C matrix."/>
a9c1d76b90c4 planemo upload for repository https://github.com/maxplanck-ie/HiCExplorer/tree/master/galaxy/wrapper/ commit 4d61b6bf2fed275ab38c226d0c4390b095a38251
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39 <param argument="--maxDepth" type="integer" value="100000"
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40 label="Maximum window length (in bp) to be considered to the left and to the right of each Hi-C bin."
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41 help="This number should around 6-10 times as large as the bin size of the Hi-C matrix."/>
a9c1d76b90c4 planemo upload for repository https://github.com/maxplanck-ie/HiCExplorer/tree/master/galaxy/wrapper/ commit 4d61b6bf2fed275ab38c226d0c4390b095a38251
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42 <param argument="--step" type="integer" value="10000"
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43 label="Step size when moving from minDepth to maxDepth"
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44 help="The step size grows exponentially as maxDeph + (step * int(x)**1.5) for x in [0, 1, ...]
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45 until it reaches maxDepth. For example, selecting step=10,000, minDepth=20,000
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46 and maxDepth=150,000 will compute TAD-scores for window sizes:
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47 20,000, 30,000, 40,000, 70,000 and 100,000"/>
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48 <conditional name="multiple_comparison_conditional">
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49 <param name="multiple_comparison_selector" type="select" label="Multiple Testing Corrections" >
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50 <option value="fdr" selected="True">False discovery rate</option>
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51 <option value="bonferroni">Bonferroni correction</option>
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52 <option value="None">No correction</option>
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53 </param>
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54 <when value="fdr">
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55 <param name="threshold" type="float" value="0.01" label="q-value" />
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56 </when>
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57 <when value="bonferroni">
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58 <param name="threshold" type="float" value="0.01" label="p-value" />
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59 </when>
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60 <when value="None" />
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61 </conditional>
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62 <param argument="--delta" type="float" value="0.001" optional="True"
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63 label="Minimum threshold of the difference between the TAD-separation score of a putative boundary and the mean of the TAD-sep. score of surrounding bins."
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64 help="The delta value reduces spurious boundaries that are shallow, which usually
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65 occur at the center of large TADs when the TAD-sep. score is flat. Higher
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66 delta threshold values produce more conservative boundary estimations. By
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67 default, multiple delta thresholds are saved for the following delta
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68 values: 0.001, 0.01, 0.03, 0.05, 0.1. Other single or multiple values
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69 can be given."/>
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70
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71 <param argument="--minBoundaryDistance" type="integer" value="" optional="True"
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72 label="Minimum distance between boundaries (in bp)."
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73 help="This parameter can be used to reduce spurious boundaries caused by noise. "/>
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75 </inputs>
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76 <outputs>
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77
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78 <data name="boundaries" from_work_dir="galaxy_tad_prefix_boundaries.bed" format="bed"
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79 label="${tool.name} on ${on_string}: Boundary positions" />
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80
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81 <data name="score" from_work_dir="galaxy_tad_prefix_score.bedgraph" format="bedgraph"
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82 label="${tool.name} on ${on_string}: Matrix with multi-scale TAD scores" />
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83 <data name="domains" from_work_dir="galaxy_tad_prefix_domains.bed" format="bed"
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84 label="${tool.name} on ${on_string}: TAD domains" />
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85 <data name="boundaries_bin" from_work_dir="galaxy_tad_prefix_boundaries.gff"
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86 format="gff" label="${tool.name} on ${on_string}: Boundary information plus score" />
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87
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88 <data name="tad_score" from_work_dir="galaxy_tad_prefix_tad_score.bm"
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89 format="bedgraph" label="${tool.name} on ${on_string}: TAD information in bm file" />
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90
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91 <data name="matrix_output" from_work_dir="galaxy_tad_prefix_zscore_matrix.h5"
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92 format="h5" label="${tool.name} on ${on_string}: Z-score matrix in h5" />
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93 </outputs>
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94 <tests>
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95 <test>
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96 <param name="matrix_h5_cooler" value="small_test_matrix.h5"/>
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97
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98 <param name="minDepth" value="60000"/>
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99 <param name="maxDepth" value="180000"/>
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100 <param name="step" value="20000"/>
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101 <param name="minBoundaryDistance" value="20000" />
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102 <conditional name="multiple_comparison_conditional">
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103 <param name="multiple_comparison_selector" value="fdr"/>
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104 <param name="threshold" value="0.1" />
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105 </conditional>
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106 <output name="boundaries" file="find_TADs/multiFDR_boundaries.bed" ftype="bed" compare="sim_size" delta="35000" />
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107 <output name="boundaries_bin" file="find_TADs/multiFDR_boundaries.gff" ftype="gff" compare="sim_size" delta="35000" />
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108 <output name="domains" file="find_TADs/multiFDR_domains.bed" ftype="bed" compare="sim_size" delta="35000" />
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109 <output name="score" file="find_TADs/multiFDR_score.bedgraph" ftype="bedgraph" compare="sim_size" delta="35000" />
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110 <output name="tad_score" file="find_TADs/multiFDR_tad_score.bm" ftype="bedgraph" compare="sim_size" delta="35000" />
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111 </test>
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112 </tests>
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113 <help><![CDATA[
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114 Calculate Topologic Associated Domains
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115 ======================================
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116
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117 Toplogical domains (TADs) are large mainly self-interacting domains. Chromatin interactions occur with higher frequency within a TAD as between TADs. More information_.
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118
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119 _________________
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120
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121 Usage
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122 -----
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123
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124 This tool must be used on unmerged matrices (restiction enzyme resolution) produced by ``hicBuildMatrix`` and corrected by ``hicCorrectMatrix``.
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125
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126 _________________
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127
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128 Computation details
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129 -------------------
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130
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131 **hicFindTADs** computes the TAD regions in two steps: in a first step it computes a TAD-separation score based on a z-score matrix for all bins. The z-score is defined as:
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132
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133 “The absolute value of z represents the distance between the raw score and the population mean in
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134 units of the standard deviation. z is negative when the raw score is below the mean, positive when above.”
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135 [Source_].
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136
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137 .. image:: $PATH_TO_IMAGES/z-score.svg
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138 :width: 100
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139
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140 `Source of image <https://wikimedia.org/api/rest_v1/media/math/render/svg/5ceed701c4042bb34618535c9a902ca1a937a351>`_
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141
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142 In our case the distribution describes the counts per bin of a genomic distance. In a second step the local minima of the TAD-separation score is evaluated with respect to the surrounding bins to assign a p-value. Two multiple testing corrections can be applied to filter the results: `Bonferroni <https://en.wikipedia.org/wiki/Bonferroni_correction>`_ or the `false discovery rate <https://en.wikipedia.org/wiki/False_discovery_rate>`_.
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143
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144 _________________
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145
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146 Output
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147 ------
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148
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149 **hicFindTADs** produces multiple outputs:
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150
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151 - TAD boundaries positions as a BED file and TAD separation score.
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152 - TAD boundaries positions with delta, p-value and TAD separation score as GFF.
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153 - TAD domains as a BED file.
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154 - TAD seperation score as bigwig (bw), bedgraph and numpy array (npz) format. These files can be used to plot the so-called TAD insulation score or TAD separation score along the genome or at specific regions. This score is much more reliable across samples than the number of TADs or the TADs width that can vary depending on the sequencing depth because of the lack of information at certain bins, and depending on the parameters used with this tool.
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155 - Matrix with multi-scale TAD scores as a bed-matrix (bm) file that can be plotted inside ``hicPlotTADs`` to nicely display TAD insulation score alongside Hi-C heatmap and other datasets.
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156 - Z-score matrix in h5 format that is useful to quickly test the --thresholdComparisons, --delta and --correctForMultipleTesting parameters by using the --TAD_sep_score_prefix option pointing to this zscore_matrix.h5 file (will be added in a future update).
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157
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158 _________________
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159
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160 Usage hints
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161 -----------
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162
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163 It is mandatory to test multiple parameters of TAD calling with **hicFindTADs** before making conclusions about the number of TADs in a given sample or before comparing TAD calling between multiple conditions. In order to compare numerous TAD calling parameters at once, it is recommended to use ``hicPlotTADs``, below you can find a plot where multiple TAD calling parameters are displayed for *Drosophila melanogaster* embryos:
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164
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165 .. image:: $PATH_TO_IMAGES/hicFindTADs_TAD_calling_comparison.png
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166 :width: 65 %
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167
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168 We can see that the fourth set of **hicFindTADs** parameters with a threshold of 0.001 gives the best results in terms of TAD calling compared to the corrected Hi-C counts distribution and compared to the enrichment of H3K36me3, which is known to be enriched at TAD boundaries in *Drosophila melanogaster*.
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169
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170 _________________
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172 For more information about HiCExplorer please consider our documentation on readthedocs.io_
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174 .. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html
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175 .. _Source: https://en.wikipedia.org/wiki/Standard_score#Calculation_from_raw_score
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176 .. _information: https://en.wikipedia.org/wiki/Topologically_associating_domain_
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177 ]]></help>
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178 <expand macro="citations" />
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179 </tool>