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annotate hifiasm.xml @ 12:da9d8bf98802 draft

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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
author bgruening
date Fri, 24 Feb 2023 17:34:21 +0000
parents cd7936c5a9a5
children ec9e21e9c71b
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1 <tool id="hifiasm" name="Hifiasm" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>haplotype-resolved de novo assembler for PacBio Hifi reads</description>
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3 <macros>
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4 <token name="@TOOL_VERSION@">0.18.8</token>
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5 <token name="@VERSION_SUFFIX@">1</token>
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6 <token name="@FORMATS@">fasta,fasta.gz,fastq,fastq.gz</token>
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7 <xml name="reads">
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8 <param name="reads" type="data" format="@FORMATS@" multiple="true" label="Input reads" />
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9 </xml>
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10 </macros>
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11 <requirements>
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12 <requirement type="package" version="@TOOL_VERSION@">hifiasm</requirement>
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13 <requirement type="package" version="0.1">yak</requirement>
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14 </requirements>
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15 <version_command>hifiasm --version</version_command>
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16 <command detect_errors="exit_code">
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17
0
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18 <![CDATA[
1
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19 #set $input_files = list()
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20 #set $hap1_inputs = list()
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21 #set $hap2_inputs = list()
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22 #set $hic1_inputs = list()
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23 #set $hic2_inputs = list()
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24 #set $ultralong_inputs = list()
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25 #for idx, read in enumerate($mode.reads):
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26 #set $inputfile = 'input_%d.%s' % ($idx, $read.dataset.extension)
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27 ln -s '$read' $inputfile &&
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28 $input_files.append($inputfile)
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29 #end for
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30 #set $input_filenames = ' '.join($input_files)
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31 #if str($hic_partition.hic_partition_selector) == 'set'
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32 mkdir HiCF HiCR &&
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33 #for idx, read in enumerate($hic_partition.h1):
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34 #set $inputfile = './HiCF/input_%d.%s' % ($idx, $read.dataset.extension)
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35 ln -s '$read' $inputfile &&
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36 $hic1_inputs.append($inputfile)
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37 #end for
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38 #for idx, read in enumerate($hic_partition.h2):
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39 #set $inputfile = './HiCR/input_%d.%s' % ($idx, $read.dataset.extension)
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40 ln -s '$read' $inputfile &&
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41 $hic2_inputs.append($inputfile)
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42 #end for
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43 #end if
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44 #if str($ont_integration.ont_integration_selector) == 'set'
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45 mkdir ultralong &&
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46 #for idx, read in enumerate($ont_integration.ul):
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47 #set $inputfile = './ultralong/input_%d.%s' % ($idx, $read.dataset.extension)
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48 ln -s '$read' $inputfile &&
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49 $ultralong_inputs.append($inputfile)
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50 #end for
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51 #end if
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52 #if str($mode.mode_selector) == 'trio':
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53 #for idx, read in enumerate($mode.hap1_reads):
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54 #set $inputfile = 'hap1_input_%d.%s' % ($idx, $read.dataset.extension)
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55 ln -s '$read' $inputfile &&
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56 $hap1_inputs.append($inputfile)
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57 #end for
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58 #for idx, read in enumerate($mode.hap2_reads):
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59 #set $inputfile = 'hap2_input_%d.%s' % ($idx, $read.dataset.extension)
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60 ln -s '$read' $inputfile &&
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61 $hap2_inputs.append($inputfile)
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62 #end for
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63 #set $hap1_filenames = ' '.join($hap1_inputs)
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64 #set $hap2_filenames = ' '.join($hap2_inputs)
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65 yak count -k$mode.yak_kmer_length -b$filter_bits -t\${GALAXY_SLOTS:-1} -o hap1.yak $hap1_filenames &&
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66 yak count -k$mode.yak_kmer_length -b$filter_bits -t\${GALAXY_SLOTS:-1} -o hap2.yak $hap2_filenames &&
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67 #end if
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68 hifiasm
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69 -t \${GALAXY_SLOTS:-1}
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70 -o output
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71 -f $filter_bits
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72 #if str($advanced_options.advanced_selector) == 'set':
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73 -k $advanced_options.hifiasm_kmer_length
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74 -w $advanced_options.window_size
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75 -D $advanced_options.drop_kmers
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76 -N $advanced_options.max_overlaps
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77 -r $advanced_options.correction_rounds
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78 #if $advanced_options.min_hist_cnt:
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79 --min-hist-cnt $advanced_options.min_hist_cnt
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80 #end if
4
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81 --max-kocc $advanced_options.max_kocc
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82 #if $advanced_options.hg_size
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83 --hg-size $advanced_options.hg_size
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
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diff changeset
84 #end if
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
85
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
86 #end if
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
87 #if str($assembly_options.assembly_selector) == 'set':
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
88 -a $assembly_options.cleaning_rounds
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
89 -z $assembly_options.adapter_length
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
90 -m $assembly_options.pop_contigs
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
91 -p $assembly_options.pop_unitigs
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
92 -n $assembly_options.remove_tips
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
93 -x $assembly_options.max_overlap
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
94 -y $assembly_options.min_overlap
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
95 $assembly_options.disable_post_join
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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parents: 1
diff changeset
96 $assembly_options.ignore_error_corrected
4
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
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97 #if $assembly_options.hom_cov
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
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diff changeset
98 --hom-cov $assembly_options.hom_cov
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
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99 #end if
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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100 #end if
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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101 #if str($mode.mode_selector) == 'trio':
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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102 -1 hap1.yak
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
103 -2 hap2.yak
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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104 -c $mode.max_kmers
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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105 -d $mode.min_kmers
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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106 #end if
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
107 #if str($purge_options.purge_selector) == 'set':
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
108 -l $purge_options.purge_level
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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109 -s $purge_options.similarity_threshold
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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110 -O $purge_options.minimum_overlap
4
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
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111 #if $purge_options.purge_max:
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
112 --purge-max $purge_options.purge_max
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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113 #end if
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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114 #if $purge_options.n_hap:
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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115 --n-hap $purge_options.n_hap
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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116 #end if
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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117 #end if
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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diff changeset
118
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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diff changeset
119 #if str($hic_partition.hic_partition_selector) == 'set':
11
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
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120 --h1 ${ ','.join(["'%s'" % $x for $x in $hic1_inputs]) }
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
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121 --h2 ${ ','.join(["'%s'" % $x for $x in $hic2_inputs]) }
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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122 #if $hic_partition.seed:
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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diff changeset
123 --seed $hic_partition.seed
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
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124 #end if
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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diff changeset
125 #if $hic_partition.n_weight:
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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126 --n-weight $hic_partition.n_weight
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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127 #end if
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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128 #if $hic_partition.n_perturb:
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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129 --n-perturb $hic_partition.n_perturb
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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130 #end if
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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131 #if $hic_partition.f_perturb:
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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132 --f-perturb $hic_partition.f_perturb
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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133 #end if
3
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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diff changeset
134 --l-msjoin $hic_partition.l_msjoin
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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135 #end if
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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136
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
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137 #if str($ont_integration.ont_integration_selector) == 'set':
11
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
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diff changeset
138 --ul ${ ','.join(["'%s'" % $x for $x in $ultralong_inputs]) }
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
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139 #if $ont_integration.ul_rate:
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
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140 --ul-rate $ont_integration.ul_rate
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
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diff changeset
141 #end if
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
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diff changeset
142 #if $ont_integration.ul_tip:
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
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diff changeset
143 --ul-tip $ont_integration.ul_tip
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
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diff changeset
144 #end if
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
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parents: 9
diff changeset
145 #end if
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
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diff changeset
146
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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diff changeset
147 ## Changed the default outputs of hifiasm. Hifiasm outputs a primary assembly and two balanced haplotypes in default. Incorporated the option '--primary' to output primary assembly and alternate assembly.
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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148 --primary
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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diff changeset
149 $input_filenames
3
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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150 #if $log_out:
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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151 2> output.log
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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152 #end if
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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153
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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154 && mkdir noseq_files && mv *.noseq.gfa noseq_files
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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155
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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diff changeset
156 #if $bins_out:
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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diff changeset
157 && mkdir bin_files && mv *.bin bin_files
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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diff changeset
158 #end if
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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159 ]]>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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160 </command>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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161 <inputs>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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162 <conditional name="mode">
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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163 <param name="mode_selector" type="select" label="Assembly mode">
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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164 <option value="standard">Standard</option>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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165 <option value="trio">Trio mode</option>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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diff changeset
166 </param>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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diff changeset
167 <when value="standard">
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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168 <expand macro="reads" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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169 </when>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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170 <when value="trio">
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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171 <expand macro="reads" />
1
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
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172 <param name="hap1_reads" type="data" format="fastq,fastq.gz" multiple="true" label="Haplotype 1 reads" />
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173 <param name="hap2_reads" type="data" format="fastq,fastq.gz" multiple="true" label="Haplotype 2 reads" />
0
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174 <param name="max_kmers" argument="-c" type="integer" value="2" label="Lower bound of the binned k-mer's frequency" />
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175 <param name="min_kmers" argument="-d" type="integer" value="5" label="Upper bound of the binned k-mer's frequency" />
2
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176 <param name="yak_kmer_length" type="integer" min="0" max="64" value="31" label="Yak counter k-mer length" />
0
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177 </when>
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178 </conditional>
5
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179 <param name="filter_bits" argument="-f" type="integer" min="0" value="37" label="Bits for bloom filter" help="A value of 0 disables the bloom filter" />
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180 <conditional name="assembly_options">
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181 <param name="assembly_selector" type="select" label="Assembly options">
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182 <option value="blank">Leave default</option>
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183 <option value="set">Specify</option>
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184 </param>
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185 <when value="blank" />
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186 <when value="set">
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187 <param name="cleaning_rounds" argument="-a" type="integer" value="4" label="Cleaning rounds" />
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188 <param name="adapter_length" argument="-z" type="integer" min="0" value="0" label="Length of adapters to be removed" />
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189 <param name="pop_contigs" argument="-m" type="integer" value="10000000" label="Minimum contig bubble size" help="Pop contig graph bubbles smaller than this value" />
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190 <param name="pop_unitigs" argument="-p" type="integer" value="100000" label="Minimum unitig bubble size" help="Pop unitig graph bubbles smaller than this value" />
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191 <param name="remove_tips" argument="-n" type="integer" value="3" label="Tip unitigs" help="Keep only tip unitigs with a number of reads greater than or equal to this value" />
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192 <param name="max_overlap" argument="-x" type="float" min="0" max="1" value="0.8" label="Maximum overlap drop ratio" help="This option is used with -r. Given a node N in the assembly graph, let max(N) be the length of the largest overlap of N. Hifiasm iteratively drops overlaps of N if their length/max(N) are below a threshold controlled by -x. Hifiasm applies -r rounds of short overlap removal with an increasing threshold between -x and -y"/>
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193 <param name="min_overlap" argument="-y" type="float" min="0" max="1" value="0.2" label="Minimum overlap drop ratio" help="This option is used with -r. Given a node N in the assembly graph, let max(N) be the length of the largest overlap of N. Hifiasm iteratively drops overlaps of N if their length/max(N) are over a threshold controlled by -y. Hifiasm applies -r rounds of short overlap removal with an increasing threshold between -x and -y"/>
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194 <param name="disable_post_join" argument="-u" type="boolean" truevalue="-u" falsevalue="" label="Skip post join contigs step" help="May improve N50" />
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195 <param name="ignore_error_corrected" argument="-i" type="boolean" truevalue="-i" falsevalue="" value="False" label="Ignore error corrected reads and overlaps" help="Ignore error corrected reads and overlaps saved in prefix.*.bin files. Apart from assembly graphs, hifiasm also outputs three binary files that save alloverlap information during assembly step. With these files, hifiasm can avoid the time-consuming all-to-all overlap calculation step, and do the assembly directly and quickly. This might be helpful when users want to get an optimized assembly by multiple rounds of experiments with different parameters." />
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196 <param argument="--hom-cov" type="integer" optional="True" value="" label="Homozygous read coverage" />
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197 </when>
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198 </conditional>
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199 <conditional name="purge_options">
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200 <param name="purge_selector" type="select" label="Options for purging duplicates">
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201 <option value="blank">Leave default</option>
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202 <option value="set">Specify</option>
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203 </param>
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204 <when value="blank" />
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205 <when value="set">
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206 <param name="purge_level" argument="-l" type="select" label="Purge level">
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207 <option value="0" selected="true">None (0)</option>
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208 <option value="1">Light (1)</option>
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209 <option value="2">Aggressive (2)</option>
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210 <option value="3">Aggressive - high heterozygosity rate (3)</option>
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211 </param>
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212 <param name="similarity_threshold" argument="-s" type="float" min="0" max="1" value="0.75" label="Similarity threshold for duplicate haplotigs" />
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213 <param name="minimum_overlap" argument="-O" type="integer" value="1" label="Minimum overlapped reads for duplicate haplotigs" />
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214 <param argument="--purge-max" type="integer" optional="true" label="Coverage upper bound" help="If not set, this will be determined automatically" />
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215 <param argument="--n-hap" type="integer" min="0" value="" optional="true" label="Assumtion of haplotype number" help="A haplotype is defined as the combination of alleles for different polymorphisms that occur on the same chromosome." />
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216 </when>
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217 </conditional>
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218 <conditional name="hic_partition">
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219 <param name="hic_partition_selector" type="select" label="Options for Hi-C-partition">
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220 <option value="blank">Leave default</option>
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221 <option value="set">Specify</option>
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222 </param>
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223 <when value="blank" />
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224 <when value="set">
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225 <param argument="--h1" type="data" format="fastq,fastq.gz" multiple="true" label="Hi-C R1 reads" />
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226 <param argument="--h2" type="data" format="fastq,fastq.gz" multiple="true" label="Hi-C R2 reads" />
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227 <param argument="--seed" type="integer" min="1" value="" optional="true" label="RNG seed" />
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228 <param argument="--n-weight" type="integer" min="1" value="" optional="true" label="Rounds of reweighting Hi-C links. Increasing this may improves phasing results but takes longer time" />
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229 <param argument="--n-perturb" type="integer" min="1" value="" optional="true" label="Rounds of perturbation. Increasing this may improves phasing results but takes longer time" />
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230 <param argument="--f-perturb" type="float" min="0" max="1" value="" optional="true" label="Fraction to flip for perturbation. Increasing this may improves phasing results but takes longer time" />
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231 <param argument="--l-msjoin" type="integer" min="0" value="500000" label="Detect misjoined unitigs of greater than or equal to specified size" help="A value of 0 disables this filter"/>
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232 </when>
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233 </conditional>
10
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234 <conditional name="ont_integration">
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235 <param name="ont_integration_selector" type="select" label="Options for ONT integration">
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236 <option value="blank">Leave default</option>
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237 <option value="set">Specify</option>
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238 </param>
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239 <when value="blank" />
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240 <when value="set">
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241 <param argument="--ul" type="data" format="fastq,fastq.gz,fasta,fasta.gz" multiple="true" label="Ultra-long (>10kb) reads" />
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242 <param argument="--ul-rate" type="float" value="0.2" optional="true" label="error rate of ultra-long reads (default 0.2)" />
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243 <param argument="--ul-tip" type="integer" value="6" optional="true" label="remove tip unitigs composed of less than (INT) reads for the UL assembly (default 6)" />
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244 </when>
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245 </conditional>
0
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246 <conditional name="advanced_options">
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247 <param name="advanced_selector" type="select" label="Advanced options">
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248 <option value="blank">Leave default</option>
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249 <option value="set">Specify</option>
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250 </param>
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251 <when value="blank" />
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252 <when value="set">
4
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253 <param name="hifiasm_kmer_length" argument="-k" type="integer" min="0" max="64" value="51" label="Hifiasm k-mer length" />
0
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254 <param name="window_size" argument="-w" type="integer" min="0" value="51" label="Minimizer window size" />
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255 <param name="drop_kmers" argument="-D" type="float" value="5.0" label="Drop k-mers" help="K-mers that occur more than this value multiplied by the coverage will be discarded" />
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256 <param name="max_overlaps" argument="-N" type="integer" value="100" label="Maximum overlaps to consider" help="The software selects the larger of this value and the k-mer count multiplied by coverage" />
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257 <param name="correction_rounds" argument="-r" type="integer" value="3" label="Correction rounds" />
2
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258 <param argument="--min-hist-cnt" type="integer" min="0" value="" optional="true" label="Minimum count threshold" help="When analyzing the k-mer spectrum, ignore counts below this value" />
4
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259 <param argument="--max-kocc" type="integer" min="0" value="20000" label="Maximum k-mer ocurrence" help="Employ k-mers occurring less than INT times to rescue repetitive overlaps" />
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260 <param argument="--hg-size" type="text" value="" optional="true" label="Estimated haploid genome size"
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261 help="Estimated haploid genome size used for inferring read coverage. If not provided, this parameter will be infered by hifism. Common suffices are required, for example, 100m or 3g">
4
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262 <sanitizer invalid_char="">
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263 <valid initial="string.digits">
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264 <add value="k" />
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265 <add value="K" />
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266 <add value="m" />
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267 <add value="M" />
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268 <add value="G" />
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269 <add value="g" />
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270 </valid>
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271 </sanitizer>
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272 <validator type="regex">[0-9kKmMGg]+</validator>
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273 </param>
0
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274 </when>
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275 </conditional>
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276 <param name="log_out" type="boolean" label="Output log file?" truevalue="yes" falsevalue="no" />
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277 <param name="bins_out" type="boolean" label="Output .bin files (used for development and debugging)?" truevalue="yes" falsevalue="no" />
0
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278 </inputs>
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279 <outputs>
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280 <!--Standard mode-->
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281 <data name="raw_unitigs" format="gfa1" from_work_dir="output.r_utg.gfa" label="${tool.name} on ${on_string}: haplotype-resolved raw unitig graph for pseudohaplotype assembly">
5
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282 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'blank'</filter>
0
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283 </data>
11
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284 <data name="processed_unitigs" format="gfa1" from_work_dir="output.p_utg.gfa" label="${tool.name} on ${on_string}: processed unitig graph for pseudohaplotype assembly">
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285 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'blank'</filter>
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286 </data>
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287 <data name="primary_contig_graph" format="gfa1" from_work_dir="output.p_ctg.gfa" label="${tool.name} on ${on_string}: primary assembly contig graph for pseudohaplotype assembly">
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288 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'blank'</filter>
0
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289 </data>
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290 <data name="alternate_contig_graph" format="gfa1" from_work_dir="output.a_ctg.gfa" label="${tool.name} on ${on_string}: alternate assembly contig graph for pseudohaplotype assembly">
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291 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'blank'</filter>
0
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292 </data>
5
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293 <!--Trio outputs without Hi-c reads-->
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294 <data name="hap1_contigs" format="gfa1" from_work_dir="output.dip.hap1.p_ctg.gfa" label="${tool.name} on ${on_string}: hap1.p_ctg contig graph">
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295 <filter>mode['mode_selector'] == 'trio' and hic_partition['hic_partition_selector'] == 'blank'</filter>
0
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296 </data>
5
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297 <data name="hap2_contigs" format="gfa1" from_work_dir="output.dip.hap2.p_ctg.gfa" label="${tool.name} on ${on_string}: hap2.p_ctg contig graph">
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298 <filter>mode['mode_selector'] == 'trio' and hic_partition['hic_partition_selector'] == 'blank'</filter>
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299 </data>
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300 <data name="raw_unitigs_trio" format="gfa1" from_work_dir="output.dip.r_utg.gfa" label="${tool.name} on ${on_string}: haplotype-resolved raw unitig graph for HiC-phased assembly">
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301 <filter>mode['mode_selector'] == 'trio' and hic_partition['hic_partition_selector'] == 'blank'</filter>
0
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302 </data>
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303 <data name="processed_unitigs_trio" format="gfa1" from_work_dir="output.dip.p_utg.gfa" label="${tool.name} on ${on_string}: haplotype-resolved processed unitig graph for HiC-phased assembly">
5
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304 <filter>mode['mode_selector'] == 'trio' and hic_partition['hic_partition_selector'] == 'blank'</filter>
2
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305 </data>
5
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306 <!-- Stardand mode with Hi-C partition outputs -->
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307 <data name="hic_pcontig_graph" format="gfa1" from_work_dir="output.hic.p_ctg.gfa" label="${tool.name} ${on_string}: Hi-C primary contig graph">
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308 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'set'</filter>
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309 </data>
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310 <data name="hic_acontig_graph" format="gfa1" from_work_dir="output.hic.a_ctg.gfa" label="${tool.name} ${on_string}: Hi-C alternate contig graph">
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311 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'set'</filter>
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312 </data>
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313 <data name="hic_balanced_contig_hap1_graph" format="gfa1" from_work_dir="output.hic.hap1.p_ctg.gfa" label="${tool.name} ${on_string}: Hi-C hap1 balanced contig graph hap1">
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314 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'set'</filter>
0
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315 </data>
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316 <data name="hic_balanced_contig_hap2_graph" format="gfa1" from_work_dir="output.hic.hap2.p_ctg.gfa" label="${tool.name} ${on_string}: Hi-C hap2 balanced contig graph hap2">
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317 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'set'</filter>
2
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318 </data>
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319 <data name="hic_raw_initig" format="gfa1" from_work_dir="output.hic.r_utg.gfa" label="${tool.name} ${on_string}: Hi-C raw unitig">
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320 <filter>mode['mode_selector'] == 'standard' and hic_partition['hic_partition_selector'] == 'set'</filter>
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321 </data>
5
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322 <!--Trio outputs with Hi-c reads-->
11
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323 <data name="hap1_contigs_hic" format="gfa1" from_work_dir="output.hic.bench.r_utg.gfa" label="${tool.name} on ${on_string}: raw unitig graph">
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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324 <filter>mode['mode_selector'] == 'trio' and hic_partition['hic_partition_selector'] == 'set'</filter>
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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325 </data>
11
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326 <data name="hap2_contigs_hic" format="gfa1" from_work_dir="output.hic.bench.p_utg.gfa" label="${tool.name} on ${on_string}: processsed unitig graph">
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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327 <filter>mode['mode_selector'] == 'trio' and hic_partition['hic_partition_selector'] == 'set'</filter>
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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328 </data>
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329 <!--Log, noseq, and bin output-->
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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330 <data name="log_file" format="txt" from_work_dir="output.log" label="${tool.name} ${on_string}: log file">
3
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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331 <filter>log_out</filter>
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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332 </data>
9
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333 <collection name="noseq_files" type="list" label="${tool.name} on ${on_string}: noseq files">
8
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334 <discover_datasets pattern="__name_and_ext__" format="gfa1" directory="noseq_files" />
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
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335 </collection>
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336 <collection name="bin_files" type="list" label="${tool.name} on ${on_string}: bin files">
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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337 <filter>bins_out</filter>
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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338 <discover_datasets pattern="__name_and_ext__" format="gfa1" directory="bin_files" />
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
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339 </collection>
0
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340 </outputs>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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341 <tests>
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342 <!-- TEST 1 -->
8
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343 <test expect_num_outputs="5">
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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344 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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345 <param name="filter_bits" value="0" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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346 <param name="mode_selector" value="standard" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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347 <output name="raw_unitigs" file="hifiasm-out1-raw.gfa" ftype="gfa1" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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348 <output name="processed_unitigs" file="hifiasm-out1-processed.gfa" ftype="gfa1" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
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349 <output name="primary_contig_graph" file="hifiasm-out1-primary.gfa" ftype="gfa1" />
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
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350 </test>
10
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diff changeset
351 <!-- TEST 2 -->
8
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352 <test expect_num_outputs="5">
1
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
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353 <param name="reads" value="hifiasm-in2-0.fa.gz,hifiasm-in2-1.fa.gz,hifiasm-in2-2.fa.gz,hifiasm-in2-3.fa.gz,hifiasm-in2-4.fa.gz" ftype="fasta.gz" />
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
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354 <param name="filter_bits" value="0" />
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
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355 <param name="mode_selector" value="standard" />
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
bgruening
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356 <output name="raw_unitigs" file="hifiasm-out2-raw.gfa" ftype="gfa1" />
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
bgruening
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357 <output name="processed_unitigs" file="hifiasm-out2-processed.gfa" ftype="gfa1" />
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
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358 <output name="primary_contig_graph" file="hifiasm-out2-primary.gfa" ftype="gfa1" />
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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359 </test>
10
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diff changeset
360 <!-- TEST 3: Test logfile out-->
8
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diff changeset
361 <test expect_num_outputs="6">
3
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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362 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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363 <param name="filter_bits" value="0" />
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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diff changeset
364 <param name="mode_selector" value="standard" />
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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diff changeset
365 <param name="log_out" value="yes"/>
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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366 <output name="raw_unitigs" file="hifiasm-out1-raw.gfa" ftype="gfa1" />
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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367 <output name="processed_unitigs" file="hifiasm-out1-processed.gfa" ftype="gfa1" />
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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diff changeset
368 <output name="primary_contig_graph" file="hifiasm-out1-primary.gfa" ftype="gfa1" />
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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369 <output name="log_file" ftype="txt">
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
bgruening
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diff changeset
370 <assert_contents>
9
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
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diff changeset
371 <has_text text="-o output -f 0 --primary input_0.fasta.gz"/>
3
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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372 </assert_contents>
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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373 </output>
9ef6920c3089 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 7132f1d3fddf9c07282e69b3c0d5c71f61d24947"
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374 </test>
10
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diff changeset
375 <!--TEST 4: Test Hi-C reads-->
8
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bgruening
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diff changeset
376 <test expect_num_outputs="6">
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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377 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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378 <param name="filter_bits" value="0" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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379 <param name="mode_selector" value="standard" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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380 <conditional name="hic_partition">
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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381 <param name="hic_partition_selector" value="set"/>
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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382 <param name="h1" value="hic_1.fastq.gz"/>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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diff changeset
383 <param name="h2" value="hic_2.fastq.gz"/>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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diff changeset
384 <param name="n_weight" value="1"/>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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385 <param name="n_perturb" value="1"/>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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386 <param name="l_perturb" value="0"/>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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387 <param name="l_msjoin" value="0"/>
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
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diff changeset
388 </conditional>
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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diff changeset
389 <output name="hic_pcontig_graph" file="hifiasm-out-hifi-p.gfa" ftype="gfa1" />
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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390 <output name="hic_acontig_graph" file="hifiasm-out-hifi-a.gfa" ftype="gfa1" />
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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diff changeset
391 <output name="hic_balanced_contig_hap1_graph" ftype="gfa1">
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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392 <assert_contents>
6
5bec28269d95 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 6817ef65c64c0800e21815822c38beba52075f75"
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393 <has_text_matching expression="^S" />
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394 <has_size value="83914" delta="500"/>
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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395 </assert_contents>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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396 </output>
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
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diff changeset
397 <output name="hic_balanced_contig_hap1_graph" ftype="gfa1" >
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
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diff changeset
398 <assert_contents>
6
5bec28269d95 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 6817ef65c64c0800e21815822c38beba52075f75"
bgruening
parents: 5
diff changeset
399 <has_text_matching expression="^S" />
9
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
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diff changeset
400 <has_size value="83914" delta="500"/>
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
401 </assert_contents>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
402 </output>
7
366a11d316a7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit e11987ed5bffa0f2aa60d28acfb9301ac87d4dc4
bgruening
parents: 6
diff changeset
403 <output name="hic_raw_initig" ftype="gfa1" >
366a11d316a7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit e11987ed5bffa0f2aa60d28acfb9301ac87d4dc4
bgruening
parents: 6
diff changeset
404 <assert_contents>
366a11d316a7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit e11987ed5bffa0f2aa60d28acfb9301ac87d4dc4
bgruening
parents: 6
diff changeset
405 <has_text_matching expression="^S" />
9
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
bgruening
parents: 8
diff changeset
406 <has_size value="83904" delta="500"/>
7
366a11d316a7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit e11987ed5bffa0f2aa60d28acfb9301ac87d4dc4
bgruening
parents: 6
diff changeset
407 </assert_contents>
366a11d316a7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit e11987ed5bffa0f2aa60d28acfb9301ac87d4dc4
bgruening
parents: 6
diff changeset
408 </output>
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
409 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
410 <!-- TEST 5: Test trio mode -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
411 <test expect_num_outputs="5">
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
412 <param name="filter_bits" value="0"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
413 <conditional name="mode">
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
414 <param name="mode_selector" value="trio"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
415 <param name="reads" value="child.fasta.gz"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
416 <param name="hap1_reads" value="paternal.fasta.gz"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
417 <param name="hap2_reads" value="maternal.fasta.gz"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
418 <param name="max_kmers" value="2"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
419 <param name="min_kmers" value="5"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
420 </conditional>
9
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
bgruening
parents: 8
diff changeset
421 <assert_command>
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
bgruening
parents: 8
diff changeset
422 <has_text text="-1 hap1.yak" />
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
bgruening
parents: 8
diff changeset
423 <has_text text="-2 hap2.yak" />
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
bgruening
parents: 8
diff changeset
424 <has_text text="--primary" />
c388a21dcbf1 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 590539b6db0f8ef16d31c2023a22aa97143bf024
bgruening
parents: 8
diff changeset
425 </assert_command>
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
426 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
427 <!-- TEST 6: Test ignore-error-corrected option -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
428 <test expect_num_outputs="5">
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
429 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
430 <param name="filter_bits" value="0" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
431 <param name="mode_selector" value="standard" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
432 <conditional name="assembly_options">
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
433 <param name="assembly_selector" value="set"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
434 <param name="ignore_error_corrected" value="True"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
435 </conditional>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
436 <output name="raw_unitigs" file="hifiasm-out3-raw.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
437 <output name="processed_unitigs" file="hifiasm-out3-processed.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
438 <output name="primary_contig_graph" file="hifiasm-out3-primary.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
439 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
440 <!-- TEST 7: Test expected haplotype number -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
441 <test expect_num_outputs="5">
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
442 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
443 <param name="filter_bits" value="0" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
444 <param name="mode_selector" value="standard" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
445 <conditional name="purge_options">
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
446 <param name="purge_selector" value="set"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
447 <param name="n_hap" value="1"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
448 </conditional>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
449 <output name="raw_unitigs" file="hifiasm-out4-raw.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
450 <output name="processed_unitigs" file="hifiasm-out4-processed.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
451 <output name="primary_contig_graph" file="hifiasm-out4-primary.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
452 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
453 <!-- TEST 8: Test min_hist_cnt option -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
454 <test expect_num_outputs="5">
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
455 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
456 <param name="filter_bits" value="0" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
457 <param name="mode_selector" value="standard" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
458 <conditional name="advanced_options">
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
459 <param name="advanced_selector" value="set"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
460 <param name="min_hist_cnt" value="1"/>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
461 </conditional>
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
462 <output name="raw_unitigs" file="hifiasm-out5-raw.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
463 <output name="processed_unitigs" file="hifiasm-out5-processed.gfa" ftype="gfa1" />
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
464 <output name="primary_contig_graph" file="hifiasm-out5-primary.gfa" ftype="gfa1" />
1
6505bd37670d "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1122ef1b9a407a781d1416d07b272016d16a6a85"
bgruening
parents: 0
diff changeset
465 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
466 <!-- TEST 9: Test max_kooc option -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
467 <test expect_num_outputs="5">
4
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
468 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
469 <param name="filter_bits" value="0" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
470 <param name="mode_selector" value="standard" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
471 <conditional name="advanced_options">
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
472 <param name="advanced_selector" value="set"/>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
473 <param name="max_kooc" value="21000"/>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
474 </conditional>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
475 <output name="raw_unitigs" file="hifiasm-out6-raw.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
476 <output name="processed_unitigs" file="hifiasm-out6-processed.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
477 <output name="primary_contig_graph" file="hifiasm-out6-primary.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
478 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
479 <!-- TEST 10: Test hg-size option -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
480 <test expect_num_outputs="5">
4
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
481 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
482 <param name="filter_bits" value="0" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
483 <param name="mode_selector" value="standard" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
484 <conditional name="advanced_options">
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
485 <param name="advanced_selector" value="set"/>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
486 <param name="hg_size" value="1k"/>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
487 </conditional>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
488 <output name="raw_unitigs" file="hifiasm-out7-raw.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
489 <output name="processed_unitigs" file="hifiasm-out7-processed.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
490 <output name="primary_contig_graph" file="hifiasm-out7-primary.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
491 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
492 <!-- TEST 11: Test ignore-error-corrected option -->
8
5f625c63b8bc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 10f1ab4414ebb4693bfb7a7ded4633e393009bed
bgruening
parents: 7
diff changeset
493 <test expect_num_outputs="5">
4
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
494 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
495 <param name="filter_bits" value="0" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
496 <param name="mode_selector" value="standard" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
497 <conditional name="assembly_options">
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
498 <param name="assembly_selector" value="set"/>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
499 <param name="hom-cov" value="1000"/>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
500 </conditional>
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
501 <output name="raw_unitigs" file="hifiasm-out8-raw.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
502 <output name="processed_unitigs" file="hifiasm-out8-processed.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
503 <output name="primary_contig_graph" file="hifiasm-out8-primary.gfa" ftype="gfa1" />
3f7be05a1597 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 8d76914ac4407512b1691a1ddd3d95dda6c80962"
bgruening
parents: 3
diff changeset
504 </test>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
505 <!-- TEST 12: test nanopore input -->
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
506 <test expect_num_outputs="5">
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
507 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
508 <param name="mode_selector" value="standard" />
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
509 <param name="filter_bits" value="0" />
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
510 <conditional name="ont_integration">
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
511 <param name="ont_integration_selector" value="set" />
11
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
512 <param name="ul" value="nanopore.fasta.gz" />
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
513 <param name="ul_tip" value="1" />
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
514 </conditional>
11
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
515 <output name="primary_contig_graph" file="hifiasm-out11-primary.gfa" ftype="gfa1" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
516 <output name="raw_unitigs" file="hifiasm-out11-raw.gfa" ftype="gfa1" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
517 </test>
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
518 <!-- TEST 13: test multi-file nanopore input -->
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
519 <test expect_num_outputs="6">
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
520 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
521 <param name="mode_selector" value="standard" />
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
522 <param name="filter_bits" value="0" />
11
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
523 <param name="log_out" value="yes" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
524 <conditional name="ont_integration">
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
525 <param name="ont_integration_selector" value="set" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
526 <param name="ul" value="nanopore.fasta.gz,nanopore.fasta.gz" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
527 <param name="ul_tip" value="1" />
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
528 </conditional>
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
529 <output name="log_file" ftype="txt">
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
530 <assert_contents>
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
531 <has_text text="--ul ./ultralong/input_0.fasta.gz,./ultralong/input_1.fasta.gz"/>
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
532 </assert_contents>
cd7936c5a9a5 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit c8948d0cc178c85b078e8789304831cee5b3fa9f
bgruening
parents: 10
diff changeset
533 </output>
10
2061fb3b81b4 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 0fb623fa67741a34587f9e5b110f22f68f0b59fa
bgruening
parents: 9
diff changeset
534 </test>
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
535 <!-- TEST 14: test bin files -->
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
536 <test expect_num_outputs="6">
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
537 <param name="reads" value="hifiasm-in1.fa.gz" ftype="fasta.gz" />
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
538 <param name="filter_bits" value="0" />
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
539 <param name="mode_selector" value="standard" />
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
540 <param name="bins_out" value="yes" />
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
541 <output_collection name="bin_files" type="list" count="3" />
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
542 </test>
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
543 </tests>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
544 <help><![CDATA[
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
545 .. class:: infomark
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
546
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
547 **HiFiASM - a fast de novo assembler**
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
548
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
549
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
550 Hifiasm is a fast haplotype-resolved *de novo* assembler for PacBio Hifi reads. It can assemble a human genome in several hours and works with the California redwood genome, one of the most complex genomes sequenced so far. Hifiasm can produce primary/alternate assemblies of quality competitive with the best assemblers. It also introduces a new graph binning algorithm and achieves the best haplotype-resolved assembly given trio data.
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
551
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
552 ----
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
553
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
554 .. class:: infomark
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
555
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
556 **Assembly mode**
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
557
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
558 - *Standard*: Standard assembly can be run in pseudohaplotype mode, or with Hi-C phasing using Hi-C reads from the same individual.
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
559 - *Trio*: When parental short reads are available, hifiasm can generate a pair of haplotype-resolved assemblies with trio binning.
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
560
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
561 ----
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
562
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
563 .. class:: infomark
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
564
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
565 **Outputs**
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
566
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
567 Non-Trio assembly:
2
f3c89da3af16 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 5a03c44d52d545bd23e651bfba1cc73050eec9a0"
bgruening
parents: 1
diff changeset
568
12
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
569 - Haplotype-resolved raw unitig graph: This graph keeps all haplotype information, including somatic mutations and recurrent sequencing errors.
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
570 - Haplotype-resolved processed unitig graph without small bubbles: This graph 'pops' small bubbles in the raw unitig graph; small bubbles might be caused by somatic mutations or noise in data, which are not the real haplotype information.
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
571 - Primary assembly contig graph: This graph includes a complete assembly with long stretches of phased blocks, though there may be some haplotype collapse.
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
572 - Alternate assembly contig graph: This graph consists of all contigs that are discarded from the primary contig graph.
da9d8bf98802 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 2bb01c64e79df856fbcb12afde62f7c14a5f59fa
bgruening
parents: 11
diff changeset
573 - [hap1]/[hap2] contig graph: Each graph consists of phased contigs (output only with Hi-C phasing enabled).
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
574
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
575
5
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
576 Trio assembly:
045c7c3d8e59 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit 1e4c6c2e48f5e95beed75bb76134cbf7fa55dc8a"
bgruening
parents: 4
diff changeset
577
0
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
578 - Haplotype-resolved raw unitig graph in GFA format . This graph keeps all haplotype information.
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
579 - Phased paternal/haplotype1 contig graph. This graph keeps the phased paternal/haplotype1 assembly.
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
580 - Phased maternal/haplotype2 contig graph. This graph keeps the phased maternal/haplotype2 assembly.
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
581
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
582
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
583 ]]></help>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
584 <citations>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
585 <citation type="doi">10.1038/s41592-020-01056-5</citation>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
586 </citations>
bf0a4667e3ce "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/hifiasm commit a118fad06e94683813e214af107fedd5fd80500a"
bgruening
parents:
diff changeset
587 </tool>