Mercurial > repos > bgruening > minced
diff minced.xml @ 0:20f12e7c279b draft
Imported from capsule None
author | bgruening |
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date | Mon, 05 May 2014 04:23:26 -0400 |
parents | |
children | 6117712107bf |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/minced.xml Mon May 05 04:23:26 2014 -0400 @@ -0,0 +1,119 @@ +<tool id="crispr_recognition_tool" name="detect CRISPR sequences" version="0.1.5"> + <description>(minced)</description> + <parallelism method="multi" split_inputs="input" split_mode="to_size" split_size="50" merge_outputs="output,output_region,output_ggf_full,output_ggf,output_fasta" /> + <requirements> + <requirement type="package" version="0.1.5">minced</requirement> + <requirement type="set_environment">MINCED_SCRIPT_PATH</requirement> + </requirements> + <stdio> + <exit_code range=":-1" level="fatal" /> + <exit_code range="1:" level="fatal" /> + </stdio> + <command> + temp_output=\$(mktemp); + minced + -minNR $minNR + -minRL $minRL + -maxRL $maxRL + -minSL $minSL + -maxSL $maxSL + #if str($searchWL) != 'none': + -searchWL $searchWL + #end if + $input + \$temp_output + #if '-spacers' in str($outputs).split(): + -spacers + #end if + #if '-gffFull' in str($outputs).split(): + -gffFull + #end if + #if '-gff' in str($outputs).split(): + -gff + #end if + ; + + python \$MINCED_SCRIPT_PATH/reformat.py \$temp_output results.bed results.tab; + #if '-spacers' in str($outputs).split(): + mv \$temp_output'_spacers.fa' $output_fasta; + #end if + </command> + <inputs> + <param format="fasta" name="input" type="data" label="Input sequence as FASTA"/> + + <param name="minNR" type="integer" value="3" label="Minimum number of repeats a CRISPER must have"/> + <param name="minRL" type="integer" value="19" label="Minimum length of a CRISPER repeated region"/> + <param name="maxRL" type="integer" value="38" label="Maximum length of a CRISPER repeated region"/> + <param name="minSL" type="integer" value="19" label="Minimum length of CRISPER non repeated region" help="or spacer region"/> + <param name="maxSL" type="integer" value="48" label="Maximum length of CRISPER non repeated region" help="or spacer region"/> + <param name="searchWL" type="select" label="Length of search window to used to discover CRISPERs"> + <option value="none" selected="true">default</option> + <option value="6">6</option> + <option value="7">7</option> + <option value="8">8</option> + <option value="9">9</option> + </param> + + <param name="outputs" type="select" multiple="True" label="Additional output format" help="Please select a output format."> + <option value="-gff">summary results in gff format</option> + <option value="BED" selected="true">summary results in BED format</option> + <option value="-gffFull">detailed results in gff format</option> + <option value="TABULAR" selected="true">detailed results in tabular format</option> + <option value="-spacers">fasta formatted file containing the spacers</option> + <validator type="no_options" message="Please select at least one output file." /> + </param> + + </inputs> + <outputs> + <data format="tabular" name="output" from_work_dir="results.tab" label="${tool.name} on ${on_string}"> + <filter>'TABULAR' in outputs</filter> + </data> + <data format="bed" name="output_region" from_work_dir="results.bed" label="${tool.name} on ${on_string} - BED file"> + <filter>'BED' in outputs</filter> + </data> + <data format="gff3" name="output_ggf_full" from_work_dir="results_full.gff" label="${tool.name} on ${on_string} - GFF file extended"> + <filter>'-gffFull' in outputs</filter> + </data> + <data format="gff3" name="output_gff" from_work_dir="results.gff" label="${tool.name} on ${on_string} - GFF file"> + <filter>'-gff' in outputs</filter> + </data> + <data format="fasta" name="output_fasta" label="${tool.name} on ${on_string} - GFF file"> + <filter>'-spacers' in outputs</filter> + </data> + </outputs> + <tests> + <test> + <param name="input" value="sequence.fasta" ftype="fasta" /> + <param name="minNR" value="3" /> + <param name="minRL" value="29" /> + <param name="maxRL" value="38" /> + <param name="minSL" value="19" /> + <param name="maxSL" value="48" /> + <param name="searchWL" value="none" /> + <output name="output" file="minced.tabular" ftype="tabular" /> + <output name="output_region" file="minced.bed" ftype="bed" /> + </test> + </tests> + <help> +**What it does** + +MinCED - Mining CRISPRs in Environmental Datasets + + +MinCED is a program to find Clustered Regularly Interspaced Short Palindromic +Repeats (CRISPRs) in full genomes or environmental datasets such as metagenomes, +in which sequence size can be anywhere from 100 to 800 bp. MinCED runs from the +command-line and was derived from CRT (http://www.room220.com/crt/). + +https://github.com/ctSkennerton/minced + +**Citation** + +For the underlying tool, please cite `Bland C, Ramsey TL, Sabree F, Lowe M, Brown K, Kyrpides NC, Hugenholtz P: +CRISPR Recognition Tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats. BMC Bioinformatics. 2007 Jun 18;8(1):209` + +If you use this tool in Galaxy, please cite Gruening, BA et al. https://github.com/bgruening/galaxytools + + </help> +</tool> +