sailfish: sailfish.xml annotate

annotate sailfish.xml @ 0:3b4ed0e473dc draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty

author	bgruening
date	Fri, 16 Oct 2015 15:09:03 -0400
parents
children	06646e81c543

rev	line source
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	1 <tool id="sailfish" name="Sailfish" version="0.7.6.0">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	2 <description>transcript quantification from RNA-seq data</description>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	3 <requirements>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	4 <requirement type="package" version="0.7.6">sailfish</requirement>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	5 </requirements>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	6 <macros>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	7 <xml name="strandedness">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	8 <param name="strandedness" type="select" label="Specify the strandedness of the reads">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	9 <option value="U" selected="True">Not stranded</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	10 <option value="SF">read 1 (or single-end read) comes from the forward strand</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	11 <option value="SR">read 1 (or single-end read) comes from the reverse strand</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	12 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	13 </xml>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	14 </macros>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	15 <stdio>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	16 <exit_code range="1:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	17 <exit_code range=":-1" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	18 <regex match="Error:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	19 <regex match="Exception:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	20 </stdio>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	21 <version_command>sailfish -version</version_command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	22 <command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	23 <![CDATA[
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	24
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	25 #if $refTranscriptSource.TranscriptSource == "history":
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	26 sailfish index
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	27 --transcripts $refTranscriptSource.ownFile
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	28 --kmerSize $refTranscriptSource.kmerSize
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	29 --out ./index_dir
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	30 --threads "\${GALAXY_SLOTS:-4}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	31 #set $index_path = './index_dir'
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	32 #else:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	33 #set $index_path = $refTranscriptSource.index.fields.path
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	34 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	35
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	36 &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	37
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	38 #if $single_or_paired.single_or_paired_opts == 'single':
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	39 ln -s $single_or_paired.input_singles ./single.$single_or_paired.input_singles.ext &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	40 #else:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	41 ln -s $single_or_paired.input_mate1 ./mate1.$single_or_paired.input_mate1.ext &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	42 ln -s $single_or_paired.input_mate2 ./mate2.$single_or_paired.input_mate2.ext &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	43 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	44
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	45
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	46 #if $geneMap:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	47 ln -s "$geneMap" ./geneMap.$geneMap.ext &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	48 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	49
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	50 sailfish quant
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	51 --index $index_path
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	52 #if $single_or_paired.single_or_paired_opts == 'single':
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	53 --libType "${single_or_paired.orientation}${single_or_paired.strandedness}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	54 --unmated_reads ./single.$single_or_paired.input_singles.ext
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	55 #else:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	56 --mates1 ./mate1.$single_or_paired.input_mate1.ext
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	57 --mates2 ./mate2.$single_or_paired.input_mate2.ext
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	58 --libType "${single_or_paired.orientation}${single_or_paired.strandedness}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	59 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	60 --output ./
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	61 $biasCorrect
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	62 --threads "\${GALAXY_SLOTS:-4}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	63
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	64 #if $fldMean:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	65 --fldMean $fldMean
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	66 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	67
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	68 #if $fldSD:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	69 --fldSD $fldSD
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	70 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	71
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	72 #if $maxReadOcc:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	73 --maxReadOcc $maxReadOcc
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	74 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	75
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	76 #if $geneMap:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	77 --geneMap ./geneMap.${geneMap.ext}
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	78 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	79
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	80 $noEffectiveLengthCorrection
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	81 $useVBOpt
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	82 $allowOrphans
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	83
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	84 $unsmoothedFLD
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	85 --maxFragLen ${maxFragLen}
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	86 --txpAggregationKey "${txpAggregationKey}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	87
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	88 ]]>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	89 </command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	90 <inputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	91 <conditional name="refTranscriptSource">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	92 <param name="TranscriptSource" type="select" label="Select a reference transcriptome from your history or use a built-in index?" help="Built-ins were indexed using default options">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	93 <option value="indexed">Use a built-in index</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	94 <option value="history" selected="True">Use one from the history</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	95 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	96 <when value="indexed">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	97 <param name="index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	98 <options from_data_table="sailfish_indexes">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	99 <filter type="sort_by" column="2"/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	100 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	101 </options>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	102 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	103 </when> <!-- build-in -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	104 <when value="history">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	105 <param name="ownFile" type="data" format="fasta" metadata_name="dbkey" label="Select the reference transcriptome" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	106 <param argument="kmerSize" type="integer" value="21" max="32" label="The size of the k-mer on which the index is built"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	107 help="There is a tradeoff here between the distinctiveness of the k-mers and their robustness to errors.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	108 The shorter the k-mers, the more robust they will be to errors in the reads, but the longer the k-mers,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	109 the more distinct they will be. We generally recommend using a k-mer size of at least 20."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	110 </when> <!-- history -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	111 </conditional> <!-- refTranscriptSource -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	112
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	113 <conditional name="single_or_paired">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	114 <param name="single_or_paired_opts" type="select" label="Is this library mate-paired?">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	115 <option value="single">Single-end</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	116 <option value="paired">Paired-end</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	117 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	118 <when value="single">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	119 <param name="input_singles" type="data" format="fastq,fasta" label="FASTQ/FASTA file" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	120 <expand macro="strandedness" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	121 </when>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	122 <when value="paired">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	123 <param name="input_mate1" type="data" format="fastq,fasta" label="Mate pair 1" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	124 <param name="input_mate2" type="data" format="fastq,fasta" label="Mate pair 2" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	125 <param name="orientation" type="select" label="Relative orientation of reads within a pair">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	126 <option value="M">Mates are oriented in the same direction (M = matching)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	127 <option value="O">Mates are oriented away from each other (O = outward)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	128 <option value="I" selected="True">Mates are oriented toward each other (I = inward)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	129 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	130 <expand macro="strandedness" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	131 </when>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	132 </conditional>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	133
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	134 <param argument="--geneMap" type="data" format="tabular,gff,gtf" optional="True" label="File containing a mapping of transcripts to genes"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	135 help="Calculates the aggregated gene-level abundance estimations. This file should be eiher a GTF file or tab-delimited format
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	136 where each line contains the name of a transcript and the gene to which it belongs separated by a tab." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	137
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	138 <param argument="--biasCorrect" type="boolean" truevalue="--biasCorrect" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	139 label="Perform bias correction" help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	140
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	141 <param argument="--fldMean" type="integer" value="200" optional="True" label="Calculate effective lengths"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	142 help="If single end reads are being used for quantification, or there are an insufficient number of uniquely mapping reads when performing paired-end quantification
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	143 to estimate the empirical fragment length distribution, then use this value to calculate effective lengths."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	144
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	145 <param argument="--fldSD" type="integer" value="80" optional="True" label="Standard deviation"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	146 help="The standard deviation used in the fragment length distribution for single-end quantification or when an empirical distribution cannot be learned."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	147
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	148 <param argument="--maxReadOcc" type="integer" value="200" optional="True" label="Maximal read mapping occurence"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	149 help="Reads mapping to more than this many places won't be considered."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	150
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	151 <param argument="--noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	152 label="Disable effective length correction" help="Disables effective length correction when computing the probability that a fragment was generated from a transcript.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	153 If this flag is passed in, the fragment length distribution is not taken into account when computing this probability."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	154
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	155 <param argument="--useVBOpt" type="boolean" truevalue="--useVBOpt" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	156 label="Use Variational Bayesian EM algorithm for optimization" help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	157
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	158 <param argument="--allowOrphans" type="boolean" truevalue="--allowOrphans" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	159 label="Consider orphaned reads as valid hits when performing lightweight-alignment"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	160 help="This option will increase sensitivity (allow more reads to map and more transcripts to be detected), but may decrease specificity as orphaned alignments are more likely to be spurious."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	161
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	162 <param argument="--unsmoothedFLD" type="boolean" truevalue="--unsmoothedFLD" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	163 label="Use the un-smoothed approach to effective length correction" help="This traditional approach works by convolving the FLD with the characteristic function over each transcript."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	164
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	165 <param argument="--maxFragLen" type="integer" value="1000" optional="True"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	166 label="The maximum length of a fragment to consider when building the empirical fragment length distribution"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	167 help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	168
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	169 <param argument="--txpAggregationKey" value="gene_id" type="text" label="The key for aggregating transcripts during gene-level estimates"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	170 help="The default is the gene_id field, but other fields (e.g. gene_name) might be useful depending on the specifics of the annotation being used." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	171
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	172 </inputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	173 <outputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	174 <data name="output_quant" format="tabular" from_work_dir="quant.sf" label="${tool.name} on ${on_string} (Quantification)" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	175 <data name="output_bias_corrected_quant" format="tabular" from_work_dir="quant_bias_corrected.sf" label="${tool.name} on ${on_string} (Bias corrected Quantification)">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	176 <filter>bias_correct == '--biasCorrect'</filter>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	177 </data>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	178 <data name="output_gene_quant" format="tabular" from_work_dir="quant.genes.sf" label="${tool.name} on ${on_string} (Gene Quantification)">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	179 <filter>geneMap is True</filter>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	180 </data>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	181 </outputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	182 <tests>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	183 <test>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	184 <param name="single_or_paired_opts" value="paired" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	185 <param name="input_mate1" value="reads_1.fastq" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	186 <param name="input_mate2" value="reads_2.fastq" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	187 <param name="biasCorrect" value="True" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	188 <param name="TranscriptSource" value="history" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	189 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	190 <output file="sailfish_quant_result1.tab" ftype="tabular" name="output_quant" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	191 <output file="sailfish_bias_result1.tab" ftype="tabular" name="output_bias_corrected_quant" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	192 </test>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	193 </tests>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	194 <help>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	195 <![CDATA[
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	196 What it does
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	197
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	198 Sailfish is a tool for transcript quantification from RNA-seq data. It
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	199 requires a set of target transcripts (either from a reference or _de-novo_
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	200 assembly) to quantify. All you need to run Sailfish is a fasta file containing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	201 your reference transcripts and a (set of) fasta/fastq file(s) containing your
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	202 reads. Sailfish runs in two phases; indexing and quantification. The indexing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	203 step is independent of the reads, and only need to be run one for a particular
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	204 set of reference transcripts and choice of k (the k-mer size). The
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	205 quantification step, obviously, is specific to the set of RNA-seq reads and is
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	206 thus run more frequently.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	207
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	208 When the quantification output contains a number of columns:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	209 (1) Transcript ID,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	210 (2) Transcript Length,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	211 (3) Transcripts per Million (TPM) and
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	212 (4) Estimated number of reads (an estimate of the number of reads drawn from this transcript given the transcript’s relative abundance and length).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	213
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	214 The first two columns are self-explanatory, the next four are measures of transcript abundance and the final is a commonly used input for differential expression tools.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	215 The Transcripts per Million quantification number is computed as described in [1], and is meant as an estimate of the number of transcripts, per million observed transcripts,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	216 originating from each isoform. Its benefit over the F/RPKM measure is that it is independent of the mean expressed transcript length
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	217 (i.e. if the mean expressed transcript length varies between samples, for example, this alone can affect differential analysis based on the K/RPKM.).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	218
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	219
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	220
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	221 Fragment Library Types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	222 ======================
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	223
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	224 There are numerous library preparation protocols for RNA-seq that result in
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	225 sequencing reads with different characteristics. For example, reads can be
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	226 single end (only one side of a fragment is recorded as a read) or paired-end
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	227 (reads are generated from both ends of a fragment). Further, the sequencing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	228 reads themselves may be unstraned or strand-specific. Finally, paired-end
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	229 protocols will have a specified relative orientation. To characterize the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	230 various different typs of sequencing libraries, we've created a miniature
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	231 "language" that allows for the succinct description of the many different types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	232 of possible fragment libraries. For paired-end reads, the possible
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	233 orientations, along with a graphical description of what they mean, are
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	234 illustrated below:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	235
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	236 .. image:: ReadLibraryIllustration.png
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	237
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	238 The library type string consists of three parts: the relative orientation of
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	239 the reads, the strandedness of the library, and the directionality of the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	240 reads.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	241
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	242 The first part of the library string (relative orientation) is only provided if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	243 the library is paired-end. The possible options are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	244
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	245 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	246
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	247 I = inward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	248 O = outward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	249 M = matching
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	250
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	251 The second part of the read library string specifies whether the protocol is
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	252 stranded or unstranded; the options are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	253
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	254 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	255
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	256 S = stranded
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	257 U = unstranded
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	258
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	259 If the protocol is unstranded, then we're done. The final part of the library
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	260 string specifies the strand from which the read originates in a strand-specific
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	261 protocol — it is only provided if the library is stranded (i.e. if the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	262 library format string is of the form S). The possible values are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	263
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	264 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	265
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	266 F = read 1 (or single-end read) comes from the forward strand
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	267 R = read 1 (or single-end read) comes from the reverse strand
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	268
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	269 So, for example, if you wanted to specify a fragment library of strand-specific
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	270 paired-end reads, oriented toward each other, where read 1 comes from the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	271 forward strand and read 2 comes from the reverse strand, you would specify ``-l
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	272 ISF`` on the command line. This designates that the library being processed has
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	273 the type "ISF" meaning, I\ nward (the relative orientation), S\ tranted
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	274 (the protocol is strand-specific), F\ orward (read 1 comes from the forward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	275 strand).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	276
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	277 The single end library strings are a bit simpler than their pair-end counter
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	278 parts, since there is no relative orientation of which to speak. Thus, the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	279 only possible library format types for single-end reads are ``U`` (for
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	280 unstranded), ``SF`` (for strand-specific reads coming from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	281 and ``SR`` (for strand-specific reads coming from the reverse strand).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	282
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	283 A few more examples of some library format strings and their interpretations are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	284
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	285 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	286
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	287 IU (an unstranded paired-end library where the reads face each other)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	288
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	289 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	290
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	291 SF (a stranded single-end protocol where the reads come from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	292
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	293 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	294
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	295 OSR (a stranded paired-end protocol where the reads face away from each other,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	296 read1 comes from reverse strand and read2 comes from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	297
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	298 .. note:: Correspondence to TopHat library types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	299
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	300 The popular `TopHat <http://ccb.jhu.edu/software/tophat/index.shtml>`_ RNA-seq
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	301 read aligner has a different convention for specifying the format of the library.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	302 Below is a table that provides the corresponding sailfish/salmon library format
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	303 string for each of the potential TopHat library types:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	304
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	305
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	306 +---------------------+-------------------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	307 \| TopHat \| Salmon (and Sailfish) \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	308 +=====================+============+============+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	309 \| \| Paired-end \| Single-end \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	310 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	311 \|``-fr-unstranded`` \|``-l IU`` \|``-l U`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	312 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	313 \|``-fr-firststrand`` \|``-l ISR`` \|``-l SR`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	314 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	315 \|``-fr-secondstrand`` \|``-l ISF`` \|``-l SF`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	316 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	317
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	318 The remaining salmon library format strings are not directly expressible in terms
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	319 of the TopHat library types, and so there is no direct mapping for them.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	320
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	321
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	322 ]]>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	323 </help>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	324 </tool>

Mercurial > repos > bgruening > sailfish

annotate sailfish.xml @ 0:3b4ed0e473dc draft