# HG changeset patch # User bgruening # Date 1587829755 14400 # Node ID e3d32471da1162bb92f6204cc55e35681dfa07c7 # Parent 7fdb9d1591e2532ab03cce8d8569c67dd108e5bf "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 30fe16f7d786e4b0c43b8333853fc56c59f6c664" diff -r 7fdb9d1591e2 -r e3d32471da11 macros.xml --- a/macros.xml Tue Oct 15 12:01:08 2019 -0400 +++ b/macros.xml Sat Apr 25 11:49:15 2020 -0400 @@ -4,12 +4,10 @@ q6 - bzip2 - seqtk + seqtk salmon - samtools + samtools vpolo - numpy pandas scipy @@ -286,6 +284,7 @@ 10.1038/nmeth.4197 + + Perform dual-phase, reads or mapping-based estimation of transcript abundance from RNA-seq reads macros.xml @@ -12,15 +12,11 @@ @salalign@ #end if @salquant@ - #if $quant_type.qtype == "reads": - #if $quant_type.writeMappings: - && samtools view -S -b ./output/samout.sam > ./output/bamout.bam - #end if + #if $quant_type.qtype == "reads" and $quant_type.writeMappings: + && @bam_sort@ ./output/samout.sam #end if - #if $quant_type.qtype == "alignment": - #if $quant_type.sampleOut: - && samtools sort -@ 4 -o ./output/postSample_sorted.bam ./output/postSample.bam - #end if + #if $quant_type.qtype == "alignment" and $quant_type.sampleOut: + && @bam_sort@ ./output/postSample.bam #end if ]]> @@ -48,7 +44,7 @@ quant_type['qtype'] == "reads" and quant_type['writeMappings'] - + quant_type['qtype'] == "alignment" and quant_type['sampleOut']