Mercurial > repos > bgruening > trim_galore
annotate trim_galore.xml @ 7:8352713cf939 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 07148c518916e16602ea26473d00358fe04bc3b6-dirty
author | bgruening |
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date | Mon, 25 Jan 2016 04:44:33 -0500 |
parents | 11962ce40855 |
children | f1e71aeaa923 |
rev | line source |
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7
8352713cf939
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 07148c518916e16602ea26473d00358fe04bc3b6-dirty
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1 <tool id="trim_galore" name="Trim Galore!" version="0.4.1"> |
6
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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2 <!-- Wrapper compatible with Trim Galore! version 0.4 --> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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3 <description>adaptive quality and adapter trimmer</description> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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4 <macros> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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5 <macro name="adapter_trimming"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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6 <conditional name="trimming"> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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7 <param name="trimming_select" type="select" label="Trimming reads?"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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8 <option value="">Automatic detection</option> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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9 <option value="--illumina">Illumina universal</option> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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10 <option value="--nextera">Nextera transposase</option> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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11 <option value="--small_rna">Illumina small RNA adapters</option> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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12 <option value="user">User defined adapter trimming</option> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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13 </param> |
7
8352713cf939
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 07148c518916e16602ea26473d00358fe04bc3b6-dirty
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14 <when value=""/> |
6
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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15 <when value="--illumina"/> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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16 <when value="--nextera"/> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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17 <when value="--small_rna"/> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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18 <when value="user"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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19 <param name="adapter" type="text" value="AGATCGGAAGAGC" label="Adapter sequence to be trimmed off"> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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20 <validator type="regex" message="Adapter sequence must contain DNA characters only (A,C,T,G or N)">^[ACTGNactgn]*$</validator> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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21 </param> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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22 <yield/> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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23 </when> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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24 </conditional> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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25 </macro> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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26 <macro name="paired_adapter_trimming"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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27 |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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28 <expand macro="adapter_trimming"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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29 <param name="adapter2" type="text" optional="True" value="" label="Adapter sequence to be trimmed off read 2"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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30 <validator type="regex" message="Adapter sequence must contain DNA characters only (A,C,T,G or N)">^[ACTGNactgn]*$</validator> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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31 </param> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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32 </expand> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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33 <param name="trim1" type="boolean" truevalue="--trim1" falsevalue="" checked="False" label="Trims 1 bp off every read from its 3' end." help="" /> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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34 <param name="three_prime_clip_R1" type="integer" value="" optional="True" label="Remove N bp from the 3' end of read 1"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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35 <help>Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed. |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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36 This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality. |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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37 (--three_prime_clip_R1)</help> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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38 </param> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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39 <param name="three_prime_clip_R2" type="integer" value="" optional="True" label="Remove N bp from the 3' end of read 1"> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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40 <help>Instructs Trim Galore! to remove N bp from the 3' end of read 2 after |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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41 adapter/quality trimming has been performed. This may remove some unwanted bias from |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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42 the 3' end that is not directly related to adapter sequence or basecall quality. (--three_prime_clip_R2)</help> |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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43 </param> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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44 </macro> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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45 </macros> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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46 <requirements> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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47 <requirement type="package" version="1.8">cutadapt</requirement> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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48 </requirements> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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49 <version_command interpreter="perl">trim_galore --version</version_command> |
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50 <command> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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51 <![CDATA[ |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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52 |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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53 ## trim_galore removes .fastq and .fq file extensions of input files. |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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54 ## This is essential if Galaxy provides links to files (with real extensions) |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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55 ## but that behaviour is causing an inconsistency in output filenaming. |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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56 ## We work around this by linking every file to cwd without file extension |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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57 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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58 #if $singlePaired.sPaired == "single": |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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59 ln -s "${singlePaired.input_singles}" ./input_singles; |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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60 #elif $singlePaired.sPaired == "paired": |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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61 ln -s "${singlePaired.input_mate1}" ./input_mate1; |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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62 ln -s "${singlePaired.input_mate2}" ./input_mate2; |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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63 #else: |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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64 ln -s "${singlePaired.input_mate_pairs.forward}" ./input_mate1; |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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65 ln -s "${singlePaired.input_mate_pairs.reverse}" ./input_mate2; |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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66 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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67 |
11962ce40855
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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68 perl $__tool_directory__/trim_galore |
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69 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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70 ## we only support fastqsanger |
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71 --phred33 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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72 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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73 #if $params.settingsType == "custom": |
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74 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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75 ## default 20 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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76 --quality $params.quality |
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77 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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78 ## default 1 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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79 --stringency $params.stringency |
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80 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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81 ## default 0.1 |
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82 -e $params.error_rate |
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83 |
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84 ## default 20 |
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85 --length $params.min_length |
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86 |
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87 #if $params.clip_R1: |
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88 --clip_R1 $params.clip_R1 |
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89 #end if |
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90 |
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91 #if $params.clip_R2: |
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92 --clip_R2 $params.clip_R2 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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93 #end if |
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94 |
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95 #if $params.retain_unpaired.retain_unpaired_select == "retain_unpaired_output": |
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96 --retain_unpaired |
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97 --length_1 $params.retain_unpaired.length_1 |
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98 --length_2 $params.retain_unpaired.length_2 |
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99 #end if |
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100 |
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101 #end if |
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102 |
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103 ## RBBS specific options. |
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104 #if $rrbs.settingsType == "custom": |
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105 $rrbs.rrbs |
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106 $rrbs.non_directional |
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107 #end if |
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108 |
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109 --output_dir ./ |
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110 --suppress_warn |
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111 |
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112 #if $params.settingsType == "custom" and not $params.report: |
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113 --no_report_file |
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114 #end if |
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115 |
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116 #if $singlePaired.trimming.trimming_select == 'user': |
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117 ## default 'AGATCGGAAGAGC' |
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118 #if $singlePaired.trimming.adapter.strip() != '': |
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119 --adapter $singlePaired.trimming.adapter |
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120 #end if |
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121 #else: |
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122 $singlePaired.trimming.trimming_select |
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123 #end if |
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124 |
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125 |
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126 #if $singlePaired.three_prime_clip_R1: |
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127 --three_prime_clip_R1 $singlePaired.three_prime_clip_R1 |
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128 #end if |
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129 |
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130 #if $singlePaired.sPaired == "single": |
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131 ## input sequence |
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132 ./input_singles |
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133 #else: |
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134 --paired |
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135 |
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136 $singlePaired.trim1 |
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137 |
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138 #if $singlePaired.trimming.trimming_select == 'user': |
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139 #if $singlePaired.trimming.adapter2 and $singlePaired.trimming.adapter2.strip() != '': |
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140 --adapter2 $singlePaired.trimming.adapter2 |
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141 #end if |
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142 #end if |
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143 |
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144 #if $singlePaired.three_prime_clip_R2: |
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145 --three_prime_clip_R2 $singlePaired.three_prime_clip_R2 |
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146 #end if |
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147 |
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148 ## input sequences |
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149 ./input_mate1 |
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150 ./input_mate2 |
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151 |
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152 #end if |
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153 |
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154 ## Trim Galore! run is finished. Move the report files to the proper place |
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155 #if $params.settingsType == "custom" and $params.report: |
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156 && |
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157 cat ./*_trimming_report.txt > $report_file; |
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158 #end if |
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159 |
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160 ]]> |
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161 </command> |
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162 <inputs> |
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163 <!-- Input Parameters --> |
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164 <conditional name="singlePaired"> |
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165 <param name="sPaired" type="select" label="Is this library paired- or single-end?"> |
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166 <option value="single">Single-end</option> |
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167 <option value="paired">Paired-end</option> |
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168 <option value="paired_collection">Paired Collection</option> |
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169 </param> |
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170 <when value="single"> |
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171 <param name="input_singles" type="data" format="fastqsanger" label="Reads in FASTQ format" /> |
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172 <expand macro="adapter_trimming"/> |
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173 |
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174 <param name="three_prime_clip_R1" type="integer" value="" optional="True" label="Remove N bp from the 3' end"> |
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175 <help>Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed. |
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176 This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality. |
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177 (--three_prime_clip_R1)</help> |
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178 </param> |
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179 </when> |
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180 <when value="paired"> |
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181 <param name="input_mate1" type="data" format="fastqsanger" label="Reads in FASTQ format" /> |
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182 <param name="input_mate2" type="data" format="fastqsanger" label="Reads in FASTQ format" /> |
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183 <expand macro="paired_adapter_trimming" /> |
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184 </when> |
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185 <when value="paired_collection"> |
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186 <param name="input_mate_pairs" format="fastqsanger" type="data_collection" collection_type="paired" |
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187 label="Select a paired collection" help="See help section for an explanation of dataset collections"/> |
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188 <expand macro="paired_adapter_trimming" /> |
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189 </when> |
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190 </conditional> |
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191 |
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192 <conditional name="params"> |
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193 <param name="settingsType" type="select" label="Trim Galore! advanced settings" help="You can use the default settings or set custom values for any of Trim Galore!'s parameters."> |
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194 <option value="default">Use defaults</option> |
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195 <option value="custom">Full parameter list</option> |
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196 </param> |
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197 <when value="default" /> |
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198 <!-- Full/advanced params. --> |
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199 <when value="custom"> |
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200 <param name="quality" type="integer" value="20" label="Trim low-quality ends from reads in addition to adapter removal" |
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201 help="For more information please see below." /> |
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202 <param name="stringency" type="integer" value="1" label="Overlap with adapter sequence required to trim a sequence" /> |
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203 <param name="error_rate" type="float" value="0.1" label="Maximum allowed error rate" /> |
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204 <param name="min_length" type="integer" value="20" label="Discard reads that became shorter than length INT" /> |
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205 |
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206 <param name="clip_R1" type="integer" optional="True" min="0" label="Instructs Trim Galore! to remove INT bp from the 5' end of read 1" /> |
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207 <param name="clip_R2" type="integer" optional="True" min="0" label="Instructs Trim Galore! to remove INT bp from the 5' end of read 2" /> |
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208 |
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209 <param name="report" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Generate a report file" help="" /> |
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210 |
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211 <conditional name="retain_unpaired"> |
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212 <param name="retain_unpaired_select" type="select" label="specify if you would like to retain unpaired reads"> |
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213 <option value="no_output">Do not output unpaired reads</option> |
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214 <option value="retain_unpaired_output">Output unpaired reads</option> |
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215 </param> |
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216 <when value="no_output" /> |
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217 <!-- Output params. --> |
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218 <when value="retain_unpaired_output"> |
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219 <param name="length_1" type="integer" value="35" label="Unpaired single-end read length cutoff needed for read 1 to be written" /> |
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220 <param name="length_2" type="integer" value="35" label="Unpaired single-end read length cutoff needed for read 2 to be written" /> |
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221 </when> <!-- output --> |
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222 </conditional> <!-- retain_unpaired --> |
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223 |
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224 </when> <!-- full --> |
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225 </conditional> <!-- params --> |
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226 |
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227 <conditional name="rrbs"> |
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228 <param name="settingsType" type="select" label="RRBS specific settings"> |
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229 <option value="default">Use defaults (no RRBS)</option> |
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230 <option value="custom">Full parameter list</option> |
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231 </param> |
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232 <when value="default" /> |
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233 <!-- Full/advanced params. --> |
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234 <when value="custom"> |
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235 <param name="rrbs" type="boolean" truevalue="--rrbs" falsevalue="" checked="True" |
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236 label="Specifies that the input file was an MspI digested RRBS sample" /> |
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237 <param name="non_directional" type="boolean" truevalue="--non_directional" falsevalue="" checked="False" |
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238 label="Selecting this option for non-directional RRBS libraries" /> |
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239 </when> <!-- full --> |
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240 </conditional> <!-- params --> |
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241 |
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242 </inputs> |
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243 <outputs> |
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244 <data format="fastqsanger" name="trimmed_reads_single" from_work_dir="input_singles_trimmed.fq" label="${tool.name} on ${on_string}: trimmed reads"> |
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245 <filter>singlePaired['sPaired'] == "single"</filter> |
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246 </data> |
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247 |
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248 <collection name="trimmed_reads_paired_collection" type="paired" label="${tool.name} on ${on_string}: paired reads"> |
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249 <data name="forward" format="fastqsanger" from_work_dir="input_mate1_val_1.fq" /> |
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250 <data name="reverse" format="fastqsanger" from_work_dir="input_mate2_val_2.fq" /> |
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251 <filter>singlePaired['sPaired'] == "paired_collection"</filter> |
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252 </collection> |
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253 |
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254 <collection name="trimmed_reads_unpaired_collection" type="paired" label="${tool.name} on ${on_string}: unpaired reads"> |
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255 <data name="forward" format="fastqsanger" from_work_dir="input_mate1_unpaired_1.fq" /> |
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256 <data name="reverse" format="fastqsanger" from_work_dir="input_mate2_unpaired_2.fq" /> |
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257 <filter>params['settingsType'] == "custom"</filter> |
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258 <filter>params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"</filter> |
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259 <filter>singlePaired['sPaired'] == "paired_collection"</filter> |
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260 </collection> |
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261 |
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262 <data format="fastqsanger" name="trimmed_reads_pair1" from_work_dir="input_mate1_val_1.fq" |
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263 label="${tool.name} on ${on_string}: trimmed reads pair 1"> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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264 <filter>singlePaired['sPaired'] == "paired"</filter> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 9198b904ef37fe46007256f1734c33de6d23331b-dirty
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265 </data> |
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266 |
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267 <data format="fastqsanger" name="trimmed_reads_pair2" from_work_dir="input_mate2_val_2.fq" |
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268 label="${tool.name} on ${on_string}: trimmed reads pair 2"> |
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269 <filter>singlePaired['sPaired'] == "paired"</filter> |
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270 </data> |
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271 |
7
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272 <data format="fastqsanger" name="unpaired_reads_1" from_work_dir="input_mate1_unpaired_1.fq" |
6
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273 label="${tool.name} on ${on_string}: unpaired reads (1)"> |
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274 <filter>params['settingsType'] == "custom"</filter> |
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275 <filter>params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"</filter> |
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276 <filter>singlePaired['sPaired'] == "paired"</filter> |
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277 </data> |
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278 |
7
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279 <data format="fastqsanger" name="unpaired_reads_2" from_work_dir="input_mate2_unpaired_2.fq" |
6
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280 label="${tool.name} on ${on_string}: unpaired reads (2)"> |
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281 <filter>params['settingsType'] == "custom"</filter> |
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282 <filter>params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"</filter> |
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283 <filter>singlePaired['sPaired'] == "paired"</filter> |
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284 </data> |
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285 <data format="txt" name="report_file" label="${tool.name} on ${on_string}: report file"> |
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286 <filter>params['settingsType'] == "custom"</filter> |
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287 <filter>params['report'] == True</filter> |
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288 </data> |
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289 |
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290 </outputs> |
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291 <tests> |
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292 <test> |
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293 <param name="input_singles" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> |
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294 <param name="sPaired" value="single" /> |
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295 <param name="settingsType" value="custom" /> |
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296 <param name="report" value="true" /> |
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297 <output name="trimmed_reads_single" file="sanger_full_range_results1.fastqsanger" ftype="fastqsanger"/> |
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298 <output name="report_file" file="sanger_full_range_report_results1.txt" ftype="txt" lines_diff="2" /> |
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299 </test> |
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300 |
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301 <test> |
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302 <param name="input_singles" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> |
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303 <param name="sPaired" value="single" /> |
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304 <param name="trimming_select" value="--illumina" /> |
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305 <output name="trimmed_reads_single" file="sanger_full_range_results2.fastqsanger" ftype="fastqsanger"/> |
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306 </test> |
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307 |
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308 <test> |
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309 <param name="input_singles" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> |
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310 <param name="sPaired" value="single" /> |
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311 <param name="adapter" value="AAAGAGC" /> |
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312 <output name="trimmed_reads_single" file="sanger_full_range_results3.fastqsanger" ftype="fastqsanger"/> |
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313 </test> |
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314 |
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315 <test> |
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316 <param name="input_mate1" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> |
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317 <param name="input_mate2" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> |
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318 <param name="sPaired" value="paired" /> |
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319 <param name="settingsType" value="custom" /> |
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320 <param name="report" value="true" /> |
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321 <output name="trimmed_reads_pair1" file="paired_example_pair1_results2.fastqsanger" ftype="fastqsanger"/> |
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322 <output name="trimmed_reads_pair2" file="paired_example_pair2_results2.fastqsanger" ftype="fastqsanger"/> |
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323 <output name="report_file" file="paired_example_results2.txt" ftype="txt" lines_diff="8" /> |
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324 </test> |
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325 |
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326 <test> |
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327 <param name="input_mate_pairs"> |
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328 <collection type="paired"> |
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329 <element name="forward" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> |
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330 <element name="reverse" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> |
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331 </collection> |
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332 </param> |
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333 |
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334 <param name="sPaired" value="paired_collection" /> |
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335 <param name="settingsType" value="custom" /> |
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336 <param name="report" value="true" /> |
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337 <param name="retain_unpaired_select" value="retain_unpaired_output" /> |
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338 |
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339 <output name="report_file" file="paired_collection_example_results3.txt" ftype="txt" lines_diff="8" /> |
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340 |
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341 <output_collection name="trimmed_reads_paired_collection" type="paired"> |
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342 <element name="forward" file="paired_collection_example_pair1_results3.fastqsanger" ftype="fastqsanger"/> |
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343 <element name="reverse" file="paired_collection_example_pair2_results3.fastqsanger" ftype="fastqsanger"/> |
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344 </output_collection> |
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345 |
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346 <output_collection name="trimmed_reads_unpaired_collection" type="paired"> |
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347 <element name="forward" file="paired_collection_example_unpair1_results3.fastqsanger" ftype="fastqsanger"/> |
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348 <element name="reverse" file="paired_collection_example_unpair2_results3.fastqsanger" ftype="fastqsanger"/> |
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349 </output_collection> |
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350 |
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351 </test> |
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352 </tests> |
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353 <help> |
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354 <![CDATA[ |
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355 **What it does** |
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356 |
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357 `Trim Galore!`_ is a wrapper script to automate quality and adapter trimming as well as quality control, with some added functionality to remove biased methylation positions for RRBS sequence files (for directional, non-directional (or paired-end) sequencing). It's main features are: |
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358 |
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359 * For adapter trimming, Trim Galore! uses the first 13 bp of Illumina standard adapters ('AGATCGGAAGAGC') by default (suitable for both ends of paired-end libraries), but accepts other adapter sequence, too |
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360 * For MspI-digested RRBS libraries, Trim Galore! performs quality and adapter trimming in two subsequent steps. This allows it to remove 2 additional bases that contain a cytosine which was artificially introduced in the end-repair step during the library preparation |
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361 * For any kind of FASTQ file other than MspI-digested RRBS, Trim Galore! can perform single-pass adapter and quality trimming |
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362 * The Phred quality of basecalls and the stringency for adapter removal can be specified individually |
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363 * Trim Galore! can remove sequences if they become too short during the trimming process. For paired-end files Trim Galore! removes entire sequence pairs if one (or both) of the two reads became shorter than the set length cutoff. Reads of a read-pair that are longer than a given threshold but for which the partner read has become too short can optionally be written out to single-end files. This ensures that the information of a read pair is not lost entirely if only one read is of good quality |
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364 * Trim Galore! can trim paired-end files by 1 additional bp from the 3' end of all reads to avoid problems with invalid alignments with Bowtie 1 |
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365 |
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366 .. _Trim Galore!: http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ |
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367 |
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368 It is developed by Felix Krueger at the Babraham Institute. |
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369 ]]> |
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370 </help> |
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371 <citations></citations> |
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372 </tool> |