Mercurial > repos > bgruening > trim_galore
comparison test-data/paired_collection_example_results3.txt @ 15:084bbd8ba7b8 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 10a9de2adae04249830c880cf0c55edaa196f3f7
author | bgruening |
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date | Tue, 30 Jul 2019 06:26:49 -0400 |
parents | 80cd83b11214 |
children | cd7e644cae1d |
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14:949f01671246 | 15:084bbd8ba7b8 |
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1 | 1 |
2 SUMMARISING RUN PARAMETERS | 2 SUMMARISING RUN PARAMETERS |
3 ========================== | 3 ========================== |
4 Input filename: input_1.fastq | 4 Input filename: input_1.fastq |
5 Trimming mode: paired-end | 5 Trimming mode: paired-end |
6 Trim Galore version: 0.4.3 | 6 Trim Galore version: 0.6.3 |
7 Cutadapt version: 1.13 | 7 Cutadapt version: 2.4 |
8 Number of cores used for trimming: 1 | |
8 Quality Phred score cutoff: 20 | 9 Quality Phred score cutoff: 20 |
9 Quality encoding type selected: ASCII+33 | 10 Quality encoding type selected: ASCII+33 |
10 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) | 11 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) |
11 Maximum trimming error rate: 0.1 (default) | 12 Maximum trimming error rate: 0.1 (default) |
12 Minimum required adapter overlap (stringency): 1 bp | 13 Minimum required adapter overlap (stringency): 1 bp |
13 Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp | 14 Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp |
14 Length cut-off for read 1: 35 bp (default) | 15 Length cut-off for read 1: 35 bp (default) |
15 Length cut-off for read 2: 35 bp (default) | 16 Length cut-off for read 2: 35 bp (default) |
16 | 17 |
17 | 18 |
18 This is cutadapt 1.13 with Python 3.5.3 | 19 This is cutadapt 2.4 with Python 3.7.3 |
19 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq | 20 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq |
20 Trimming 1 adapter with at most 10.0% errors in single-end mode ... | 21 Processing reads on 1 core in single-end mode ... |
21 Finished in 0.01 s (101 us/read; 0.59 M reads/minute). | 22 Finished in 0.01 s (72 us/read; 0.83 M reads/minute). |
22 | 23 |
23 === Summary === | 24 === Summary === |
24 | 25 |
25 Total reads processed: 99 | 26 Total reads processed: 99 |
26 Reads with adapters: 52 (52.5%) | 27 Reads with adapters: 52 (52.5%) |
73 69 1 0.0 1 1 | 74 69 1 0.0 1 1 |
74 73 1 0.0 1 1 | 75 73 1 0.0 1 1 |
75 80 1 0.0 1 1 | 76 80 1 0.0 1 1 |
76 86 1 0.0 1 1 | 77 86 1 0.0 1 1 |
77 | 78 |
78 | |
79 RUN STATISTICS FOR INPUT FILE: input_1.fastq | 79 RUN STATISTICS FOR INPUT FILE: input_1.fastq |
80 ============================================= | 80 ============================================= |
81 99 sequences processed in total | 81 99 sequences processed in total |
82 | 82 |
83 | 83 |
84 SUMMARISING RUN PARAMETERS | 84 SUMMARISING RUN PARAMETERS |
85 ========================== | 85 ========================== |
86 Input filename: input_2.fastq | 86 Input filename: input_2.fastq |
87 Trimming mode: paired-end | 87 Trimming mode: paired-end |
88 Trim Galore version: 0.4.3 | 88 Trim Galore version: 0.6.3 |
89 Cutadapt version: 1.13 | 89 Cutadapt version: 2.4 |
90 Number of cores used for trimming: 1 | |
90 Quality Phred score cutoff: 20 | 91 Quality Phred score cutoff: 20 |
91 Quality encoding type selected: ASCII+33 | 92 Quality encoding type selected: ASCII+33 |
92 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) | 93 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) |
93 Maximum trimming error rate: 0.1 (default) | 94 Maximum trimming error rate: 0.1 (default) |
94 Minimum required adapter overlap (stringency): 1 bp | 95 Minimum required adapter overlap (stringency): 1 bp |
95 Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp | 96 Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp |
96 Length cut-off for read 1: 35 bp (default) | 97 Length cut-off for read 1: 35 bp (default) |
97 Length cut-off for read 2: 35 bp (default) | 98 Length cut-off for read 2: 35 bp (default) |
98 | 99 |
99 | 100 |
100 This is cutadapt 1.13 with Python 3.5.3 | 101 This is cutadapt 2.4 with Python 3.7.3 |
101 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq | 102 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq |
102 Trimming 1 adapter with at most 10.0% errors in single-end mode ... | 103 Processing reads on 1 core in single-end mode ... |
103 Finished in 0.01 s (100 us/read; 0.60 M reads/minute). | 104 Finished in 0.01 s (55 us/read; 1.08 M reads/minute). |
104 | 105 |
105 === Summary === | 106 === Summary === |
106 | 107 |
107 Total reads processed: 100 | 108 Total reads processed: 99 |
108 Reads with adapters: 59 (59.0%) | 109 Reads with adapters: 58 (58.6%) |
109 Reads written (passing filters): 100 (100.0%) | 110 Reads written (passing filters): 99 (100.0%) |
110 | 111 |
111 Total basepairs processed: 25,100 bp | 112 Total basepairs processed: 24,849 bp |
112 Quality-trimmed: 746 bp (3.0%) | 113 Quality-trimmed: 745 bp (3.0%) |
113 Total written (filtered): 23,276 bp (92.7%) | 114 Total written (filtered): 23,035 bp (92.7%) |
114 | 115 |
115 === Adapter 1 === | 116 === Adapter 1 === |
116 | 117 |
117 Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 59 times. | 118 Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. |
118 | 119 |
119 No. of allowed errors: | 120 No. of allowed errors: |
120 0-9 bp: 0; 10-12 bp: 1 | 121 0-9 bp: 0; 10-12 bp: 1 |
121 | 122 |
122 Bases preceding removed adapters: | 123 Bases preceding removed adapters: |
123 A: 11.9% | 124 A: 12.1% |
124 C: 39.0% | 125 C: 37.9% |
125 G: 8.5% | 126 G: 8.6% |
126 T: 40.7% | 127 T: 41.4% |
127 none/other: 0.0% | 128 none/other: 0.0% |
128 | 129 |
129 Overview of removed sequences | 130 Overview of removed sequences |
130 length count expect max.err error counts | 131 length count expect max.err error counts |
131 1 16 25.0 0 16 | 132 1 16 24.8 0 16 |
132 2 7 6.2 0 7 | 133 2 7 6.2 0 7 |
133 3 1 1.6 0 1 | 134 3 1 1.5 0 1 |
134 4 2 0.4 0 2 | 135 4 2 0.4 0 2 |
135 6 2 0.0 0 2 | 136 6 2 0.0 0 2 |
136 9 2 0.0 0 2 | 137 9 1 0.0 0 1 |
137 10 1 0.0 1 1 | 138 10 1 0.0 1 1 |
138 13 1 0.0 1 1 | 139 13 1 0.0 1 1 |
139 14 2 0.0 1 2 | 140 14 2 0.0 1 2 |
140 15 1 0.0 1 1 | 141 15 1 0.0 1 1 |
141 16 1 0.0 1 1 | 142 16 1 0.0 1 1 |
158 57 1 0.0 1 1 | 159 57 1 0.0 1 1 |
159 60 1 0.0 1 1 | 160 60 1 0.0 1 1 |
160 67 1 0.0 1 1 | 161 67 1 0.0 1 1 |
161 80 1 0.0 1 1 | 162 80 1 0.0 1 1 |
162 | 163 |
163 | |
164 RUN STATISTICS FOR INPUT FILE: input_2.fastq | 164 RUN STATISTICS FOR INPUT FILE: input_2.fastq |
165 ============================================= | 165 ============================================= |
166 100 sequences processed in total | 166 99 sequences processed in total |
167 | 167 |
168 Total number of sequences analysed for the sequence pair length validation: 99 | 168 Total number of sequences analysed for the sequence pair length validation: 99 |
169 | 169 |
170 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%) | 170 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%) |