Mercurial > repos > bgruening > trim_galore
diff test-data/paired_collection_example_results3gz.txt @ 10:b4e39d993fc8 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit bbef69cc08154b5c156c25f9ca43df0915803856
author | bgruening |
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date | Thu, 20 Apr 2017 09:14:30 -0400 |
parents | |
children | 80cd83b11214 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/paired_collection_example_results3gz.txt Thu Apr 20 09:14:30 2017 -0400 @@ -0,0 +1,172 @@ + +SUMMARISING RUN PARAMETERS +========================== +Input filename: input_1.fastq.gz +Trimming mode: paired-end +Trim Galore version: 0.4.0 +Cutadapt version: 1.8 +Quality Phred score cutoff: 20 +Quality encoding type selected: ASCII+33 +Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) +Maximum trimming error rate: 0.1 (default) +Minimum required adapter overlap (stringency): 1 bp +Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp +Length cut-off for read 1: 35 bp (default) +Length cut-off for read 2: 35 bp (default) +Output file will be GZIP compressed + + +This is cutadapt 1.8 with Python 3.5.3 +Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz +Trimming 1 adapter(s) with at most 10.0% errors in single-end mode ... +Finished in 0.10 s (1010 us/read; 0.06 M reads/minute). + +=== Summary === + +Total reads processed: 99 +Reads with adapters: 52 (52.5%) +Reads written (passing filters): 99 (100.0%) + +Total basepairs processed: 24,849 bp +Quality-trimmed: 205 bp (0.8%) +Total written (filtered): 23,339 bp (93.9%) + +=== Adapter 1 === + +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. + +No. of allowed errors: +0-9 bp: 0; 10-12 bp: 1 + +Bases preceding removed adapters: + A: 9.6% + C: 38.5% + G: 23.1% + T: 28.8% + none/other: 0.0% + +Overview of removed sequences +length count expect max.err error counts +1 11 24.8 0 11 +2 5 6.2 0 5 +3 3 1.5 0 3 +4 3 0.4 0 3 +12 1 0.0 1 1 +13 2 0.0 1 2 +14 1 0.0 1 1 +16 1 0.0 1 1 +17 1 0.0 1 0 1 +20 2 0.0 1 2 +21 1 0.0 1 1 +24 1 0.0 1 1 +26 2 0.0 1 2 +31 1 0.0 1 1 +33 1 0.0 1 1 +41 2 0.0 1 2 +49 1 0.0 1 1 +50 1 0.0 1 1 +54 1 0.0 1 1 +56 1 0.0 1 1 +58 2 0.0 1 2 +60 1 0.0 1 1 +67 2 0.0 1 2 +68 1 0.0 1 1 +69 1 0.0 1 1 +73 1 0.0 1 1 +80 1 0.0 1 1 +86 1 0.0 1 1 + + +RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz +============================================= +99 sequences processed in total + + +SUMMARISING RUN PARAMETERS +========================== +Input filename: input_2.fastq.gz +Trimming mode: paired-end +Trim Galore version: 0.4.0 +Cutadapt version: 1.8 +Quality Phred score cutoff: 20 +Quality encoding type selected: ASCII+33 +Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) +Maximum trimming error rate: 0.1 (default) +Minimum required adapter overlap (stringency): 1 bp +Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp +Length cut-off for read 1: 35 bp (default) +Length cut-off for read 2: 35 bp (default) +Output file will be GZIP compressed + + +This is cutadapt 1.8 with Python 3.5.3 +Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz +Trimming 1 adapter(s) with at most 10.0% errors in single-end mode ... +Finished in 0.10 s (1000 us/read; 0.06 M reads/minute). + +=== Summary === + +Total reads processed: 100 +Reads with adapters: 59 (59.0%) +Reads written (passing filters): 100 (100.0%) + +Total basepairs processed: 25,100 bp +Quality-trimmed: 746 bp (3.0%) +Total written (filtered): 23,276 bp (92.7%) + +=== Adapter 1 === + +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 59 times. + +No. of allowed errors: +0-9 bp: 0; 10-12 bp: 1 + +Bases preceding removed adapters: + A: 11.9% + C: 39.0% + G: 8.5% + T: 40.7% + none/other: 0.0% + +Overview of removed sequences +length count expect max.err error counts +1 16 25.0 0 16 +2 7 6.2 0 7 +3 1 1.6 0 1 +4 2 0.4 0 2 +6 2 0.0 0 2 +9 2 0.0 0 2 +10 1 0.0 1 1 +13 1 0.0 1 1 +14 2 0.0 1 2 +15 1 0.0 1 1 +16 1 0.0 1 1 +17 1 0.0 1 1 +19 2 0.0 1 2 +21 1 0.0 1 1 +25 1 0.0 1 1 +30 1 0.0 1 1 +32 2 0.0 1 2 +34 1 0.0 1 1 +36 2 0.0 1 2 +38 1 0.0 1 1 +40 1 0.0 1 1 +41 1 0.0 1 1 +42 1 0.0 1 1 +43 1 0.0 1 1 +49 1 0.0 1 1 +51 1 0.0 1 1 +56 1 0.0 1 1 +57 1 0.0 1 1 +60 1 0.0 1 1 +67 1 0.0 1 1 +80 1 0.0 1 1 + + +RUN STATISTICS FOR INPUT FILE: input_2.fastq.gz +============================================= +100 sequences processed in total + +Total number of sequences analysed for the sequence pair length validation: 99 + +Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%)