# HG changeset patch
# User bgruening
# Date 1633687072 0
# Node ID cd7e644cae1d49f48542d81664be041bdec896f6
# Parent 084bbd8ba7b81c67b6ad6bb0efe7d1d86ee685d3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 276a0ec327f5369c16563696047f0d31577c353f"
diff -r 084bbd8ba7b8 -r cd7e644cae1d macros.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,66 @@
+
+ 0.6.7
+ 0
+
+
+ trim-galore
+
+
+
+
+ trim_galore
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ ^[ACTGNactgn]*$
+
+
+
+
+
+
+
+
+ ^[ACTGNactgn]*$
+
+
+
+
+ Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed.
+ This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality.
+ (--three_prime_clip_R1)
+
+
+ Instructs Trim Galore! to remove N bp from the 3' end of read 2 after
+ adapter/quality trimming has been performed. This may remove some unwanted bias from
+ the 3' end that is not directly related to adapter sequence or basecall quality.
+
+
+
+
+
+ @misc{githubTrim_Galore,
+ author = {Krueger, Felix},
+ year = {2021},
+ title = {Trim Galore},
+ publisher = {GitHub},
+ journal = {GitHub repository},
+ url = {https://https://github.com/FelixKrueger/TrimGalore.com/fenderglass/Flye}}
+
+
+
+
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/mec_reads_pair1.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/mec_reads_pair1.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC
+TTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
++
+GGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA
+CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
++
+GGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A//
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF.
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT
+CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG
++
+GGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/A@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA
+CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT
++
+GG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
+@M01368:8:000000000-A3GHV:1:1104:11928:24197/1:R1:ACGTAGGT:R2:CCTCGCTC:F1:GCGA:F2:CGGG
+AAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA
++
+GGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1:R1:CACTTGGG:R2:CCTACAAG:F1:GGTA:F2:CATT
+CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT
++
+GGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC
+AGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC
++
+GGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.-
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT
+TATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC
++
+GGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC
+TGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
++
+GGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG
+TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
++
+GGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
+@M01368:8:000000000-A3GHV:1:1104:7756:4776/1:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA
+TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA
++
+GGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF/
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG
+TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
++
+GGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA
+GCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC
++
+GGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1:R1:TTAGTAAG:R2:GGTTCCTA:F1:TATG:F2:CTTC
+TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
++
+G3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/
+@M01368:8:000000000-A3GHV:1:1106:22927:3863/1:R1:GTATCCGA:R2:AAGACAGA:F1:CATC:F2:TACT
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA
++
+GGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9///
+@M01368:8:000000000-A3GHV:1:1106:23625:4236/1:R1:TAGCTAAA:R2:CACTTTAG:F1:GTGA:F2:TAAG
+CTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA
++
+GGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9---
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1:R1:GTATCCGA:R2:GCATTAAT:F1:CATC:F2:TAAT
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+GGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9
+@M01368:8:000000000-A3GHV:1:1107:14451:26748/1:R1:GTATGTTC:R2:AGCTAAAG:F1:GCCT:F2:TGAA
+TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA
++
+GGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9.
+@M01368:8:000000000-A3GHV:1:1107:18714:24788/1:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC
+TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA
++
+GGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF/
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC
+GTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
++
+GGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC
+GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG
++
+GGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A//
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA
+AAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT
++
+CFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA
+ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
++
+GGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC-
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC
++
+EGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA
+CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT
++
+GGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC
+TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC
++
+GGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBACFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT
+TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG
++
+EEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;///
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC
+CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
++
+GGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA;
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC
+TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
++
+GGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA
+AGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC
++
+GGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1:R1:ATAAATAA:R2:CATCACGA:F1:TAGG:F2:TGGA
+TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG
++
+GGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1:R1:ACTTGGGG:R2:GTCCTACA:F1:GTAG:F2:AGCA
+TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC
++
+GGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B;
+@M01368:8:000000000-A3GHV:1:1111:13195:14562/1:R1:ATCAAAGA:R2:GTATCCGA:F1:CAGA:F2:CATC
+ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA
++
+GGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BFA
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1:R1:GCCAACAC:R2:ATTCAATT:F1:GTTC:F2:GTTA
+CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
++
+GFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1:R1:GTCCTACA:R2:CGACATCT:F1:AGCA:F2:GGTT
+TAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG
++
+GGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG
+AGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG
++
+GGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>-
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA
+TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
++
+GGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A-
+@M01368:8:000000000-A3GHV:1:1111:9655:13916/1:R1:GGCCCATA:R2:TTAGTAAG:F1:ACAC:F2:TATG
+TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA
++
+GFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9/
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1:R1:GTATCCGA:R2:GTGCTCCG:F1:CATC:F2:GCTC
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT
++
+GGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A.
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1:R1:CTGTAATA:R2:GTATCCGA:F1:TTGA:F2:CATC
+CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC
++
+4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//..
+@M01368:8:000000000-A3GHV:1:1112:9832:6701/1:R1:CGCCTGTA:R2:GCTAAAGT:F1:ATAT:F2:GAAC
+GAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGA
++
+GGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG
+AATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC
++
+GGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/mec_reads_pair2.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/mec_reads_pair2.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/2:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC
+ACTCACCCACGGCGGCCATCAGCCGATACTAAGTTTGGGGTATGGTGGGGGGGATAATGAATTAGGTTGTGGGGGAGGGTTTGTGGTTGAGAGAAACACAAAAAACAATCTTATATATGGGTAGTCGTTTTGTATTGGTTTTTTGTTTTGTTTGTGTTTTGAGTGTCGGTTTAGTTCGGTGTACTAGGGGGGGTGGATGGGGTCGGCTGGTGAGGGGGTCTTAGTGTATTGAGTGTGG
++
+A111A100000000////011110//>>/12@1@22B/////1@>21/>>/-----9/;////9////--;-;-;-----;--------9/-/-///9-;-------9//////9/////-//-/9-;-;9--/////99-;--9-:-;----/---/-----////---9-/////--;A-//////---------9/-----;-----/-/-----;--;//////////9;///-
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/2:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA
+CACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAA
++
+GGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF0
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/2:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA
+TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
++
+GGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/2:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT
+GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT
++
+CGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-///F??//@BCBAC<<.<<///FDFACF?FHFGC//??1B0</@GFGD/@1FG10FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFFFFFB9/9FFFEBF9/B/BB/BFAADDFFC-;;9-;@@9-.9/://///://///...-
+@M01368:8:000000000-A3GHV:1:1103:25465:20010/2:R1:GACATAGG:R2:AACCTAAA:F1:GTGC:F2:TAGC
+CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGGTCTTGGTGGTCGTCCTCGTATTTGAACACATAATCGTG
++
+GGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFFFFFBBFFFFFFFFFFFFFFFFFADFDFFC-BBB;BF-.;9:B9B9/9/;9;//;:.99/;-...:.../;//////.9/////.;.
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/2:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA
+CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGAT
++
+FFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/2:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC
+ACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGGGGGTGCCGGCTCATTAAAAAAAGAAGATGTCGAGATGTCAGTGCGTGAGTGGAAGTG
++
+GGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0FGHHGHHGHHHHHHHHHHFGHHGGGGGHG-:@?FAGA:.:09CB0000;9B0.9-;---:./----.0;B00;:0;;.../90009..-..00000000-...90:.00/00
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/2:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT
+CTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAA
++
+GGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FBB//
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/2:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC
+TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACGAG
++
+AFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B..;.
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/2:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG
+TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
++
+GFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9
+@M01368:8:000000000-A3GHV:1:1104:7756:4776/2:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA
+TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
++
+GGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/2:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG
+TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTCC
++
+GGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900;
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/2:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA
+AACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGT
++
+GGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFFGGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F
+@M01368:8:000000000-A3GHV:1:1105:15502:11453/2:R1:GTCCTACA:R2:GATCACAG:F1:AGCA:F2:GTCT
+TCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACCTGTCTCTTATACACATCTG
++
+GGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFFE//090::B0000:009900
+@M01368:8:000000000-A3GHV:1:1105:16787:9771/2:R1:GTTATTAT:R2:TCCTACTT:F1:TATG:F2:CAGG
+TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
++
+GGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0::/.-
+@M01368:8:000000000-A3GHV:1:1105:17564:15723/2:R1:TATGCTCG:R2:ATCCGACA:F1:CCTG:F2:TCTG
+TTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT
++
+GGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9
+@M01368:8:000000000-A3GHV:1:1105:18108:12091/2:R1:TAATAGGA:R2:GTATCCGA:F1:TGAG:F2:CATC
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC
++
+GGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFFFFFBFFFBFEFFFF0
+@M01368:8:000000000-A3GHV:1:1105:18331:16683/2:R1:TCTGGTTC:R2:TTATTATG:F1:CTAC:F2:TCCT
+CAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATATCT
++
+GGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;0B
+@M01368:8:000000000-A3GHV:1:1105:20577:18421/2:R1:GGGGGTAG:R2:ATGTCCTA:F1:CTAA:F2:CAAG
+ATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTT
++
+CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB/
+@M01368:8:000000000-A3GHV:1:1106:17389:18671/2:R1:CCATAACA:R2:GTATGTTC:F1:CTTG:F2:GCCT
+TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACCCTGA
++
+GGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F
+@M01368:8:000000000-A3GHV:1:1107:18714:24788/2:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC
+TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTAGGTCGCAGGAACTGTCCTTGT
++
+GGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.;A///00--;A-.;.000000090
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/2:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+GGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BFF.
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/2:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC
+TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTCTGTCTCTTATACACATCTGACGCTGCCGCCGAGGGCTCTAGTTGTGGTCTG
++
+GGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBBBB9CFFB0C00;000;:CFFB;:...D?>:@=:9-@...//;////..;/;/
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/2:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA
+GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTTTT
++
+GGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF</ACFHHCDCDE?CGEBEGFFFGF?.?DGGGGEFEFFB00CFFFB.:AFFB;/BB9BBFFBBFFFAD.;///9.A./A:FB;BF//B..
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/2:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA
+GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAGTTAGGTTTTT
++
+DGGGGGGGGHHHHHHHFGHHHHHHGHHHGGHGGHHHHHHGHHHFHHHGGGGGHHHHHGHHHHHHHHHHHHGGGGGHGHHFGGHHHGGGCGGGHHGGG?GGFGGGHHFHGH?FG/CDHHFAAEGGG/DHFC?-.AFGFH0CHHFH0DHHG/CDEBFHG.CA.:0C:;.FGFEFFG:E.B909BFF/;CFGBB9BBA.:CFGFFFFFFFF/;//AF?.ADEAB.9.A/;.:BFFF/9FF.
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/2:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA
+ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGCGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGCCTGTCTCTTATACACATCTG
++
+GGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFFB:./9/9/9;B//9BF/;B/
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/2:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA
+ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
++
+GGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF@
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/2:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC
+GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACTGTCTCTTATACAC
++
+GGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0BFF0BBFFBFF:90:
+@M01368:8:000000000-A3GHV:1:1108:14328:14751/2:R1:ATAATAGG:R2:GTATCCGA:F1:ATGA:F2:CATC
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATCTGTCTCTTATACA
++
+GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9:BBFFFBF9B9//:/
+@M01368:8:000000000-A3GHV:1:1108:16350:22621/2:R1:TTAGTAAG:R2:CATCTGGT:F1:TATG:F2:TCCT
+CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT
++
+GGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF
+@M01368:8:000000000-A3GHV:1:1108:21791:23272/2:R1:CTTTAGTA:R2:CATAACAC:F1:AGTA:F2:TTGG
+GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCT
++
+GGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.::FBB///;/////:/
+@M01368:8:000000000-A3GHV:1:1108:23314:26089/2:R1:ATTGAACG:R2:CATAACAC:F1:TAGG:F2:TTGG
+GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCC
++
+GGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBFF/
+@M01368:8:000000000-A3GHV:1:1108:25084:18127/2:R1:TTATTATG:R2:CCCTTAAA:F1:TCCT:F2:TAAG
+CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG
++
+GGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B;
+@M01368:8:000000000-A3GHV:1:1108:7626:10048/2:R1:ATCAAAGA:R2:AACCTAAA:F1:CAGA:F2:TAGC
+CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATCTCTGGGGTCGGCGGATTT
++
+GGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFFDFFFFFB-<>;>@BE<:000.0.0/0<00<0:>..-<<<---.0
+@M01368:8:000000000-A3GHV:1:1109:11270:12255/2:R1:AATCAAAG:R2:CTACCTCA:F1:ACAG:F2:GGGT
+ATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATT
++
+GGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00009BBF
+@M01368:8:000000000-A3GHV:1:1109:24217:19077/2:R1:AAAGTGAA:R2:TAATAGGA:F1:CTGT:F2:TGAG
+CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTCTGTC
++
+FEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBFAFFAFFFFFBFFFFFFF/9BFFDFFDDFF-9@CDAD;:AFF/;B//....;;///;
+@M01368:8:000000000-A3GHV:1:1109:27103:7153/2:R1:GTAATATT:R2:NTACTTCA:F1:GAAC:F2:GGGT
+NTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTC
++
+#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFFFFE/:9/
+@M01368:8:000000000-A3GHV:1:1109:8763:7859/2:R1:TAGTAAGT:R2:GGTTCCTA:F1:ATGT:F2:CTTC
+GGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
++
+GGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB
+@M01368:8:000000000-A3GHV:1:1110:12808:18367/2:R1:AATTAACA:R2:GCCCACAC:F1:CACT:F2:GTTC
+CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTT
++
+GGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:;F0
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/2:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT
+ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACCCTGAAG
++
+GGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:FB.;//
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/2:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC
+GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
++
+GGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/2:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC
+CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT
++
+GGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9:
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/2:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA
+GGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTCTGTCTCTTATACACATTTGTCGCTC
++
+GGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF0B0:000<00FDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF/
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/2:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG
+TCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTCTGTCTCTTATACACATCTGACGCTGCCGAGA
++
+GGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFFFFFFFEFBFFFB/FFFFFFF//9A..;F--@9
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/2:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA
+GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTA
++
+GGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-DFFF.
+@M01368:8:000000000-A3GHV:1:1113:5741:16959/2:R1:TAGTAAGT:R2:GTATCCGA:F1:ATGT:F2:CATC
+GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+GGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/BF.
+@M01368:8:000000000-A3GHV:1:1114:10130:11959/2:R1:CGTTCCCC:R2:ATCAGAGA:F1:TTAA:F2:CAGA
+ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGAGTTCTGTGGGGCCGGGGATCCTTATAAAAAAAATAGA
++
+GGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAEEDG.CGCGHC0CGBFHGFBBF0ABDDEFF@?--:BB@.;:BF;0.0:0.0:000:BF.-.------.9/;000::0;0:--.000
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/2:R1:CTTTAGTA:R2:CACTTGGG:F1:AGTA:F2:GGTA
+CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCC
++
+GGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:BA.
+@M01368:8:000000000-A3GHV:1:1114:15066:16302/2:R1:TAAATAAG:R2:TTATTATT:F1:ACAT:F2:ATGT
+CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGTGG
++
+GGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0;0..--./00::B/090000000;A....
+@M01368:8:000000000-A3GHV:1:1114:16639:15258/2:R1:CCTAAATA:R2:TTATTATT:F1:GCCC:F2:ATGT
+CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG
++
+GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF0
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/2:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG
+CTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACTGTCTCTTATACACATT
++
+GGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFFFF.9//;FFFFF/BFFB/
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_collection_example_pair1_results3.fastq.gz
Binary file test-data/paired_collection_example_pair1_results3.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_collection_example_pair2_results3.fastq.gz
Binary file test-data/paired_collection_example_pair2_results3.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_collection_example_results3.txt
--- a/test-data/paired_collection_example_results3.txt Tue Jul 30 06:26:49 2019 -0400
+++ b/test-data/paired_collection_example_results3.txt Fri Oct 08 09:57:52 2021 +0000
@@ -3,11 +3,13 @@
==========================
Input filename: input_1.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -16,10 +18,10 @@
Length cut-off for read 2: 35 bp (default)
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
-Processing reads on 1 core in single-end mode ...
-Finished in 0.01 s (72 us/read; 0.83 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.01 s (115 µs/read; 0.52 M reads/minute).
=== Summary ===
@@ -33,10 +35,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 9.6%
@@ -85,11 +87,13 @@
==========================
Input filename: input_2.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -98,10 +102,10 @@
Length cut-off for read 2: 35 bp (default)
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
-Processing reads on 1 core in single-end mode ...
-Finished in 0.01 s (55 us/read; 1.08 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.02 s (159 µs/read; 0.38 M reads/minute).
=== Summary ===
@@ -115,10 +119,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 12.1%
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_collection_example_results3gz.txt
--- a/test-data/paired_collection_example_results3gz.txt Tue Jul 30 06:26:49 2019 -0400
+++ b/test-data/paired_collection_example_results3gz.txt Fri Oct 08 09:57:52 2021 +0000
@@ -3,11 +3,13 @@
==========================
Input filename: input_1.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -17,10 +19,10 @@
Output file will be GZIP compressed
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
-Processing reads on 1 core in single-end mode ...
-Finished in 0.02 s (176 us/read; 0.34 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.01 s (129 µs/read; 0.47 M reads/minute).
=== Summary ===
@@ -34,10 +36,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 9.6%
@@ -86,11 +88,13 @@
==========================
Input filename: input_2.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -100,10 +104,10 @@
Output file will be GZIP compressed
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
-Processing reads on 1 core in single-end mode ...
-Finished in 0.02 s (169 us/read; 0.36 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.04 s (395 µs/read; 0.15 M reads/minute).
=== Summary ===
@@ -117,10 +121,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 12.1%
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_collection_example_unpair1_results3.fastq.gz
Binary file test-data/paired_collection_example_unpair1_results3.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_collection_example_unpair2_results3.fastq.gz
Binary file test-data/paired_collection_example_unpair2_results3.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_example_pair1_results2.fastq.gz
Binary file test-data/paired_example_pair1_results2.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_example_pair2_results2.fastq.gz
Binary file test-data/paired_example_pair2_results2.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_example_results2.txt
--- a/test-data/paired_example_results2.txt Tue Jul 30 06:26:49 2019 -0400
+++ b/test-data/paired_example_results2.txt Fri Oct 08 09:57:52 2021 +0000
@@ -3,21 +3,23 @@
==========================
Input filename: input_1.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
-Processing reads on 1 core in single-end mode ...
-Finished in 0.01 s (75 us/read; 0.80 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.01 s (117 µs/read; 0.51 M reads/minute).
=== Summary ===
@@ -31,10 +33,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 9.6%
@@ -83,21 +85,23 @@
==========================
Input filename: input_2.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
-Processing reads on 1 core in single-end mode ...
-Finished in 0.00 s (50 us/read; 1.19 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.03 s (256 µs/read; 0.23 M reads/minute).
=== Summary ===
@@ -111,10 +115,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 12.1%
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_example_results2gz.txt
--- a/test-data/paired_example_results2gz.txt Tue Jul 30 06:26:49 2019 -0400
+++ b/test-data/paired_example_results2gz.txt Fri Oct 08 09:57:52 2021 +0000
@@ -3,11 +3,13 @@
==========================
Input filename: input_1.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -15,10 +17,10 @@
Output file will be GZIP compressed
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
-Processing reads on 1 core in single-end mode ...
-Finished in 0.03 s (287 us/read; 0.21 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.01 s (114 µs/read; 0.53 M reads/minute).
=== Summary ===
@@ -32,10 +34,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 9.6%
@@ -84,11 +86,13 @@
==========================
Input filename: input_2.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -96,10 +100,10 @@
Output file will be GZIP compressed
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
-Processing reads on 1 core in single-end mode ...
-Finished in 0.02 s (170 us/read; 0.35 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.02 s (232 µs/read; 0.26 M reads/minute).
=== Summary ===
@@ -113,10 +117,10 @@
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
No. of allowed errors:
-0-9 bp: 0; 10-12 bp: 1
+1-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 12.1%
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_hardtrimmed3_pair1_.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_hardtrimmed3_pair1_.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
+TCGCACCTACGTTCAATATT
++
+FFEFE;DFFFDFBFF/9BFB
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
+GACCTGTGATCCATCGTGAT
++
+FF//;BFFFFFBF/9D:A//
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
+TATTATTTATCGCACCTACG
++
+FFEFBFFFFFFFFFF:FFF.
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
+TTTAAGGGGAACGGGTGGGG
++
+FBFFFFFFBF..A..;@B--
+@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
+AAGGGGAACGTGTGGGCTAT
++
+FFFBFFFFFFEFFFFFEFFF
+@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
+TGATCCATCGTGATGTCTTT
++
+FFFFFBFFBD.AFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
+ACCCTATGTCGCAGTATCTG
++
+..9..9/9;BEF;A:9/BFF
+@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
+AATTCTGTCTCTTATACACA
++
+F0FFFFFFFFFFFFFFFFF:
+@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
+GTCTCTTATACACATCTCCG
++
+FBBBFFFFFBFF0BFBF9F.
+@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
+AATCTCGTATGCCGTCTTCT
++
+FF;.BF9ADF/9;A;DFFF0
+@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
+TCTGTCTTTGATTCCTGCCT
++
+BBBFBFBFFF//BFB/BFF/
+@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
+ACTAAAGTGTGTTAATTCTG
++
+FBFF0FFBBBBFBFBFFFF0
+@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
+CCTGAAGTAGGAACCAGATG
++
+FFFFBBFFFFF/FFEFFFBB
+@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
+TATTATTTATCGCACCTACG
++
+F0BFFFBBFF0EFFFFFFFA
+@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
+CCTATGTCGCAGTATCTGTC
++
+.::FBFBFFE-A9:BFFFF0
+@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
+TATTATTTATCGCACCTACG
++
+FBFFFFFFFFFFFFFFFFF?
+@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
+GATTCCTGCCTCATCCTATT
++
+FFFFFFFFBFBBBFFFFEFF
+@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
+TATTACTGTCTCTTATACAC
++
+:FFFBB/BFFFFFBFFFFB/
+@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
+TCGCAGTATCTGTCTTTGAT
++
+BFEA-A.B//9F9F//;BBB
+@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
+TGCCTCATCCTATTATTTAT
++
+FFFFFFFFFFF/FFFFBFF9
+@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
+GCGAATCTCGGATGCCGTCT
++
+FFFF@;.9BF..;-./-A=F
+@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
+AAAAAAAAAAAACAACAGAC
++
+.B/;:>---;--..;.../9
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
+GAGACTAAGGCGAATTTCGT
++
+-B?DFFF/B/B?---.//;.
+@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
+GTATCTGTCTTTGATTCCTG
++
+A./FFFFFFFFFFFF/BFF/
+@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
+TATTATTTATCGCACCTACG
++
+B/BFFFF/BFFE?EFAFEF9
+@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
+TAGGAACCAGATGTCGGATA
++
+FFFFFFFFFFFFFFFFF@D9
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
+TCTGTCTTTGATTCCTGCCT
++
+;FBBFBFB///;99999BA9
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
+ACCCAGTAGACTAGTGCGTC
++
+9...:.9//9////;/9-.-
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
+GGGTGATAGACCTGTGATCC
++
+BF./AAF/BFFBBF/F/BFB
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
+TTCCTGCCTCATCCTATTAT
++
+//;/;9/A/;/9BFFFBFBB
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
+CGTGATGTCTTATTTAAGGG
++
+F?.AFFF/BFFEFFFFFEFF
+@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
+GTCGCAGTATCTGTCTTTGA
++
+FFBEFFF:9FFFFBBFFFF/
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
+TGTCGGATACAGTTCACTTT
++
+FFBFFFFFFFFFFFFEFFF0
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
+TGAAGTAGGAACCAGATGTC
++
+FF/FFFFFFFFFFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
+TGGGCTATTTAGGTTTTATG
++
+FF?B---;B99//9/;A//9
+@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
+CTGTCTCTTATACACATCTC
++
+FFFFEFFFFFFFFBFFFBFF
+@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
+GACCTGTGATCCATCGTGAT
++
+FFFFFFBFFFFFFFFFEFF/
+@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
+TTAGGTTTTATGACCCTGAA
++
+FFBBFBBFFFFFE1:BFFB0
+@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
+GATACCTGTCTCTTATACAC
++
+F-A.B//;BFFEF//BB///
+@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
+CGCACCTACGTTCAATATTA
++
+B.DABFFFFFFB.//:FB//
+@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
+CTTTGATTCCTGCCTCATCC
++
+FBB/:FF9BF/BF.BBFF/:
+@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
+ATTCCTGCCTCATCCTATTT
++
+BFBFFBFF.0:000BFFBF0
+@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
+ACCTGTGATCCATCGTGATG
++
+FFFFFFF/9FFFFBDEFFFF
+@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
+GAACATACTTACTAAAGTGT
++
+-9@DDBFFFFFFFFBBFFF0
+@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
+TGTGGGCTATTTAGGTTTTT
++
+FE:AAFEF.BFFFFF;9FF.
+@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
+GCAGTATCTGGCTTTGATTC
++
+..;A;/B/;B//;;//BBF/
+@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
+CTCCGAGCCCACGAGACTAA
++
+//FFF-=--.99=--.9///
+@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
+ATTCCTGCCTCATCCTATTA
++
+EFFFFFFFAF///9FF/BB/
+@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
+GTATGCCGTCTTCTGCTTGA
++
+AFFFFFF;;BFE/BFFFFB:
+@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
+AACTACAACCCACGGACGCA
++
+;B.//////;...---9---
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
+TATTATTTATCGCACCTACG
++
+F/FFBFFFFFFADE>BFFF9
+@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
+TTTAGGTTTTATGACCCTGA
++
+FBFFFF;FFEBFBFFB./9.
+@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
+TTTAGGTTTTATGACCCTGA
++
+FFFFFFBFFFFFBBFFBBF/
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
+CCTGAAGTAGGAACCAGATG
++
+FFFFFFFFFFFFBFF.AA/9
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
+ACTAAGGCGAATCTCGTATG
++
+9DFFF/FFBF;-.:;.9A//
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
+TCAATATTACAGGCGAACAT
++
+FFFFFBF//:BFBACF--9/
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
+ATCCTATTATTTATCGCACC
++
+FFFFFFFFFFF/BFBEFFC-
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
+CTGTCTCTTATACACCTCTC
++
+BFFFBBFFF//B/////-;9
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
+GACCTGTGATCCATCGTGAT
++
+FFFFBF;BBFFBFFFFE?F/
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
+GAACCCTGTCTCTTATACAC
++
+EFF?EFFFF0BFFFFBBB00
+@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
+GGATACCTGTCTCTTATACA
++
+EFFFBF/BF/FFEFFBFFF/
+@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
+TGGGCTATTTAGGTTTTATG
++
+BE.99FAFFFFFFF/;EFF/
+@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
+TGTGGGCTATTTAGGTTTTA
++
+EEFEFFFBFBFBBFFFFF./
+@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
+TGAAGTAGGAACCAGATGTG
++
+BB./:BFFF/F;AA?F////
+@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
+TGTGATCCATCGTGATGTCT
++
+FF9FBFFB/F/.;FFFF/BB
+@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
+TATGCCGTCTTCTGCTTGAA
++
+AAFFFFFFAFABBFFFB/;;
+@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
+AGACTAAGGCGAATCTCGTT
++
+-@DBFFFFBFD---.;BAA.
+@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
+CTTATACACATCTCCGAGCC
++
+FFB/;F/99/FBFFBDCF;B
+@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
+GGCGAATCTCGTATGCAGTT
++
+FFFFA;;;FF..9//;/;;/
+@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
+GATACAGTCCACTTTAGCTA
++
+CCDFFFFFFF0BFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
+TGCCTCATCCTATTACTGTC
++
+9;F./B//BBFB9////99/
+@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
+AGGCGAATATCGTATGCCGT
++
+BF/99D-;.//.../9;B-.
+@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
+GACCCTGAAGTAGCTGTCTC
++
+BBFFBFFFF///;/9B;///
+@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
+GGGCTATTTAGGCTTTATGC
++
+EDAA.A/BFFFFFFFFFEB/
+@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
+AAGGGGAACGTGTGGGCCTG
++
+BFBFFB9..AF?FFFFFFF9
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
+GTATCTGTCTTTGATTCCTG
++
+9-/;B9BF/9BB//B/;///
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
+ATGACCCTGAAGTAGGAACC
++
+FFFFFBFFF.BFFFBFBBA;
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
+AGGGTGATAGACCTGTGATC
++
+/99;;:.//:FFFFBBF//9
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
+CTTATACACATCTCCGAGCC
++
+BFFFFB/BFFBFFF/ADCAD
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
+TGCTTGCAAAAAAAAAATAG
++
+/BBFFF.9/FF?DA=@9./9
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
+CTGTCTTTGATTCCTGCCTC
++
+FBBFFFF/BBFFFFBFF9B;
+@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
+ACATCTCCGAGCCCACGAGA
++
+FFEFFFFED-DCFF?.:BFA
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
+ATTAAAGTGTGTTAATTAAT
++
+B//B/;/;9//9//;B/:B9
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
+CATCGTGATGTCTTATTTAG
++
+B/FBDE9/;99/B/BFFEF/
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
+CACATCTCCGAGCCCACGAG
++
+FFFFB:FFFFD=CAEEEF>-
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
+TACGTTCAATATTACAGGCG
++
+FFFBA/F//BFFFBF/;/A-
+@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
+GTCGCAGTATCTGTCTTTGA
++
+F/B?A@F-9BBFF//BB/9/
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
+CGAATCTCGTATGCCGTCTT
++
+FAD--.99;A?////:.9A.
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
+ATGACCTTGAAGTAGGAACC
++
+FFF/BF//9/B//9:B//..
+@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
+TTTATGACCCTGAAGTAGGA
++
+FFFFFFF/B./FB///;BF/
+@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
+AGCTACCTCCAAGTGTTATG
++
+FF/BFFB/:.BB//;FBFFF
+@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
+AGACTAAGGCGAAGTTCATT
++
+;-;AFFE;/99-9--/;///
+@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
+TCGTGATGTCTTATTTAAGG
++
+FFFEABF//99FFEFFFBFF
+@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
+GGGCTATTTAGGTTTTATGA
++
+9AD;.FFFB/B/;FBA/B//
+@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
+GCAACAAACACACATCCAGA
++
+F./B/..9.9:...//////
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
+TGGGGGCTATTTAGGTTTTT
++
+;A.-=A--:BBFB90;;FE.
+@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
+TAATTATTGCTTGTAGGACA
++
+F/;BFF/;/////9/////.
+@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
+ACATACTTACTAAATTGTGT
++
+-9-99/B9F/://///99/;
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
+ATCTGTCTATTATACACATC
++
+FFFFFFFFFFFFFFFFEFF0
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_hardtrimmed3_pair2_.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_hardtrimmed3_pair2_.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
+TCGCACCTACGTTCAATATT
++
+FFEFE;DFFFDFBFF/9BFB
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
+GACCTGTGATCCATCGTGAT
++
+FF//;BFFFFFBF/9D:A//
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
+TATTATTTATCGCACCTACG
++
+FFEFBFFFFFFFFFF:FFF.
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
+TTTAAGGGGAACGGGTGGGG
++
+FBFFFFFFBF..A..;@B--
+@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
+AAGGGGAACGTGTGGGCTAT
++
+FFFBFFFFFFEFFFFFEFFF
+@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
+TGATCCATCGTGATGTCTTT
++
+FFFFFBFFBD.AFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
+ACCCTATGTCGCAGTATCTG
++
+..9..9/9;BEF;A:9/BFF
+@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
+AATTCTGTCTCTTATACACA
++
+F0FFFFFFFFFFFFFFFFF:
+@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
+GTCTCTTATACACATCTCCG
++
+FBBBFFFFFBFF0BFBF9F.
+@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
+AATCTCGTATGCCGTCTTCT
++
+FF;.BF9ADF/9;A;DFFF0
+@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
+TCTGTCTTTGATTCCTGCCT
++
+BBBFBFBFFF//BFB/BFF/
+@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
+ACTAAAGTGTGTTAATTCTG
++
+FBFF0FFBBBBFBFBFFFF0
+@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
+CCTGAAGTAGGAACCAGATG
++
+FFFFBBFFFFF/FFEFFFBB
+@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
+TATTATTTATCGCACCTACG
++
+F0BFFFBBFF0EFFFFFFFA
+@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
+CCTATGTCGCAGTATCTGTC
++
+.::FBFBFFE-A9:BFFFF0
+@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
+TATTATTTATCGCACCTACG
++
+FBFFFFFFFFFFFFFFFFF?
+@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
+GATTCCTGCCTCATCCTATT
++
+FFFFFFFFBFBBBFFFFEFF
+@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
+TATTACTGTCTCTTATACAC
++
+:FFFBB/BFFFFFBFFFFB/
+@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
+TCGCAGTATCTGTCTTTGAT
++
+BFEA-A.B//9F9F//;BBB
+@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
+TGCCTCATCCTATTATTTAT
++
+FFFFFFFFFFF/FFFFBFF9
+@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
+GCGAATCTCGGATGCCGTCT
++
+FFFF@;.9BF..;-./-A=F
+@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
+AAAAAAAAAAAACAACAGAC
++
+.B/;:>---;--..;.../9
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
+GAGACTAAGGCGAATTTCGT
++
+-B?DFFF/B/B?---.//;.
+@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
+GTATCTGTCTTTGATTCCTG
++
+A./FFFFFFFFFFFF/BFF/
+@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
+TATTATTTATCGCACCTACG
++
+B/BFFFF/BFFE?EFAFEF9
+@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
+TAGGAACCAGATGTCGGATA
++
+FFFFFFFFFFFFFFFFF@D9
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
+TCTGTCTTTGATTCCTGCCT
++
+;FBBFBFB///;99999BA9
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
+ACCCAGTAGACTAGTGCGTC
++
+9...:.9//9////;/9-.-
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
+GGGTGATAGACCTGTGATCC
++
+BF./AAF/BFFBBF/F/BFB
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
+TTCCTGCCTCATCCTATTAT
++
+//;/;9/A/;/9BFFFBFBB
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
+CGTGATGTCTTATTTAAGGG
++
+F?.AFFF/BFFEFFFFFEFF
+@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
+GTCGCAGTATCTGTCTTTGA
++
+FFBEFFF:9FFFFBBFFFF/
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
+TGTCGGATACAGTTCACTTT
++
+FFBFFFFFFFFFFFFEFFF0
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
+TGAAGTAGGAACCAGATGTC
++
+FF/FFFFFFFFFFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
+TGGGCTATTTAGGTTTTATG
++
+FF?B---;B99//9/;A//9
+@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
+CTGTCTCTTATACACATCTC
++
+FFFFEFFFFFFFFBFFFBFF
+@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
+GACCTGTGATCCATCGTGAT
++
+FFFFFFBFFFFFFFFFEFF/
+@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
+TTAGGTTTTATGACCCTGAA
++
+FFBBFBBFFFFFE1:BFFB0
+@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
+GATACCTGTCTCTTATACAC
++
+F-A.B//;BFFEF//BB///
+@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
+CGCACCTACGTTCAATATTA
++
+B.DABFFFFFFB.//:FB//
+@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
+CTTTGATTCCTGCCTCATCC
++
+FBB/:FF9BF/BF.BBFF/:
+@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
+ATTCCTGCCTCATCCTATTT
++
+BFBFFBFF.0:000BFFBF0
+@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
+ACCTGTGATCCATCGTGATG
++
+FFFFFFF/9FFFFBDEFFFF
+@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
+GAACATACTTACTAAAGTGT
++
+-9@DDBFFFFFFFFBBFFF0
+@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
+TGTGGGCTATTTAGGTTTTT
++
+FE:AAFEF.BFFFFF;9FF.
+@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
+GCAGTATCTGGCTTTGATTC
++
+..;A;/B/;B//;;//BBF/
+@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
+CTCCGAGCCCACGAGACTAA
++
+//FFF-=--.99=--.9///
+@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
+ATTCCTGCCTCATCCTATTA
++
+EFFFFFFFAF///9FF/BB/
+@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
+GTATGCCGTCTTCTGCTTGA
++
+AFFFFFF;;BFE/BFFFFB:
+@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
+AACTACAACCCACGGACGCA
++
+;B.//////;...---9---
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
+TATTATTTATCGCACCTACG
++
+F/FFBFFFFFFADE>BFFF9
+@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
+TTTAGGTTTTATGACCCTGA
++
+FBFFFF;FFEBFBFFB./9.
+@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
+TTTAGGTTTTATGACCCTGA
++
+FFFFFFBFFFFFBBFFBBF/
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
+CCTGAAGTAGGAACCAGATG
++
+FFFFFFFFFFFFBFF.AA/9
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
+ACTAAGGCGAATCTCGTATG
++
+9DFFF/FFBF;-.:;.9A//
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
+TCAATATTACAGGCGAACAT
++
+FFFFFBF//:BFBACF--9/
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
+ATCCTATTATTTATCGCACC
++
+FFFFFFFFFFF/BFBEFFC-
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
+CTGTCTCTTATACACCTCTC
++
+BFFFBBFFF//B/////-;9
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
+GACCTGTGATCCATCGTGAT
++
+FFFFBF;BBFFBFFFFE?F/
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
+GAACCCTGTCTCTTATACAC
++
+EFF?EFFFF0BFFFFBBB00
+@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
+GGATACCTGTCTCTTATACA
++
+EFFFBF/BF/FFEFFBFFF/
+@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
+TGGGCTATTTAGGTTTTATG
++
+BE.99FAFFFFFFF/;EFF/
+@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
+TGTGGGCTATTTAGGTTTTA
++
+EEFEFFFBFBFBBFFFFF./
+@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
+TGAAGTAGGAACCAGATGTG
++
+BB./:BFFF/F;AA?F////
+@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
+TGTGATCCATCGTGATGTCT
++
+FF9FBFFB/F/.;FFFF/BB
+@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
+TATGCCGTCTTCTGCTTGAA
++
+AAFFFFFFAFABBFFFB/;;
+@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
+AGACTAAGGCGAATCTCGTT
++
+-@DBFFFFBFD---.;BAA.
+@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
+CTTATACACATCTCCGAGCC
++
+FFB/;F/99/FBFFBDCF;B
+@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
+GGCGAATCTCGTATGCAGTT
++
+FFFFA;;;FF..9//;/;;/
+@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
+GATACAGTCCACTTTAGCTA
++
+CCDFFFFFFF0BFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
+TGCCTCATCCTATTACTGTC
++
+9;F./B//BBFB9////99/
+@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
+AGGCGAATATCGTATGCCGT
++
+BF/99D-;.//.../9;B-.
+@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
+GACCCTGAAGTAGCTGTCTC
++
+BBFFBFFFF///;/9B;///
+@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
+GGGCTATTTAGGCTTTATGC
++
+EDAA.A/BFFFFFFFFFEB/
+@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
+AAGGGGAACGTGTGGGCCTG
++
+BFBFFB9..AF?FFFFFFF9
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
+GTATCTGTCTTTGATTCCTG
++
+9-/;B9BF/9BB//B/;///
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
+ATGACCCTGAAGTAGGAACC
++
+FFFFFBFFF.BFFFBFBBA;
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
+AGGGTGATAGACCTGTGATC
++
+/99;;:.//:FFFFBBF//9
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
+CTTATACACATCTCCGAGCC
++
+BFFFFB/BFFBFFF/ADCAD
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
+TGCTTGCAAAAAAAAAATAG
++
+/BBFFF.9/FF?DA=@9./9
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
+CTGTCTTTGATTCCTGCCTC
++
+FBBFFFF/BBFFFFBFF9B;
+@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
+ACATCTCCGAGCCCACGAGA
++
+FFEFFFFED-DCFF?.:BFA
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
+ATTAAAGTGTGTTAATTAAT
++
+B//B/;/;9//9//;B/:B9
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
+CATCGTGATGTCTTATTTAG
++
+B/FBDE9/;99/B/BFFEF/
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
+CACATCTCCGAGCCCACGAG
++
+FFFFB:FFFFD=CAEEEF>-
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
+TACGTTCAATATTACAGGCG
++
+FFFBA/F//BFFFBF/;/A-
+@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
+GTCGCAGTATCTGTCTTTGA
++
+F/B?A@F-9BBFF//BB/9/
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
+CGAATCTCGTATGCCGTCTT
++
+FAD--.99;A?////:.9A.
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
+ATGACCTTGAAGTAGGAACC
++
+FFF/BF//9/B//9:B//..
+@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
+TTTATGACCCTGAAGTAGGA
++
+FFFFFFF/B./FB///;BF/
+@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
+AGCTACCTCCAAGTGTTATG
++
+FF/BFFB/:.BB//;FBFFF
+@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
+AGACTAAGGCGAAGTTCATT
++
+;-;AFFE;/99-9--/;///
+@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
+TCGTGATGTCTTATTTAAGG
++
+FFFEABF//99FFEFFFBFF
+@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
+GGGCTATTTAGGTTTTATGA
++
+9AD;.FFFB/B/;FBA/B//
+@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
+GCAACAAACACACATCCAGA
++
+F./B/..9.9:...//////
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
+TGGGGGCTATTTAGGTTTTT
++
+;A.-=A--:BBFB90;;FE.
+@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
+TAATTATTGCTTGTAGGACA
++
+F/;BFF/;/////9/////.
+@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
+ACATACTTACTAAATTGTGT
++
+-9-99/B9F/://///99/;
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
+ATCTGTCTATTATACACATC
++
+FFFFFFFFFFFFFFFFEFF0
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_hardtrimmed5_pair1_.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_hardtrimmed5_pair1_.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
+ATCTGGTTCCTACTTCAGGG
++
+BCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
+GTTATTATTATGTCCTACAA
++
+AAAAAFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
+GTATCCGACATCTGGTTCCT
++
+CCCCCFDDDDDFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
+ATTAATTAACACACTTTAGT
++
+33AA?DFD5BDFGGGFEBDG
+@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
+AATTAACACACTTTAGTAAG
++
+CCCCDFFFFCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
+ATTATGTCCTACAAGCATTA
++
+ABCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
+CGCTCCGGGCCCATAACACT
++
+>A@AAAAD2ADDFFGGGGGF
+@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
+CCTTAAATAAGACATCACGA
++
+ABBBBFFFFFFFGGGGGGGC
+@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
+ATATTGAACGTAGGTGCGAT
++
+BCCBCFFFFFBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
+ATCAAAGACAGATACTGCGA
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
+CACTTGGGGGTAGCTAAAGT
++
+BBBCBFF@CCBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
+GCCCACACGTTCCCCTTAAA
++
+ABCCBCCBBFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
+ATATTGAACGTAGGTGCGAT
++
+BBBBBFFFFFAFGG5GGGGG
+@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
+GTATCCGACATCTGGTTCCT
++
+AAABCFCABBCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
+CTCCGGGCCCATAACACTTG
++
+CCDCCCCCCBCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
+GTATCCGACATCTGGTTCCT
++
+CBCCCFCCCCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
+GTAGCTAAAGTGAACTGTAT
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
+GTATCCGACATCTGGTTCCT
++
+AAABAFCCCCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
+GCCCATAACACTTGGGGGTA
++
+BBBBBFFFBDFFGFBCG2EG
+@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
+AAAGCGAACTGTATCCGACA
++
+ABB?AABBBBFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
+AAGACAGATACTGCGACATA
++
+BBBBBFFFFFFFGGEEGGFG
+@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
+GACATAGGGTGCTCCGGCTC
++
+ABBBBFFFFBAAGGGFEFGG
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
+AAATAAGACATCACGATGGA
++
+3A3AAFFFFBFFGGG2CF?4
+@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
+TAACACTTGGGGGTAGCTAA
++
+CCCBCFFFFFCCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
+GTATCCGACATCTGGTTCCT
++
+BABCBFBACCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
+ACGTAGGTGCGATAAATAAT
++
+CDDCCFCFFFBCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
+CACTTGGGGGTAGCTAAAGT
++
+ABA?AFBAB2>AFGGGGFFG
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
+CACGATGGATCACAGGTCTA
++
+AAABABCAFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
+ATTCAATTGTTATTATTATG
++
+AAAABFFFFFFFGGGGGGGC
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
+AGCTAAAGTGAACTGTATCC
++
+AAA?AFFF3DFFFGGBGFF3
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
+CTACAAGCATTAATTAATTA
++
+BBBBBFFBFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
+GGCCCATAACACTTGGGGGT
++
+CCCCCCFFFFFFGGGGGGGE
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
+ATAAATAATAGGATGAGGCA
++
+CCCCBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
+ATTGAACGTAGGTGCGATAA
++
+CDDDDFFFBFFCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
+TTAGTAAGTATGTTCGCCTG
++
+AA1A1DFFFFFBGG3A111F
+@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
+GTCCTACAAGCATTAATTAA
++
+A3AAAFFFFFCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
+GTTATTATTATGTCCTACAA
++
+AABCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
+TATGCTCGCCTGTAATATTG
++
+EDCCDFFCEEDCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
+TAATAGGATGAGGCAGGAAT
++
+CCCCCFBFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
+TCTGGTTCCTACTTCAGGGT
++
+BABA?DFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
+GGGGGTAGCTAAAGTGAACT
++
+ABB@3>AAFFFFFEAGGGFG
+@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
+TAGCTAAAGTGAACTGTATC
++
+CBCBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
+TTATGATTATGTCCTACAAG
++
+BAAAAFFFBFFFGGGGGGFF
+@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
+AACCTAAATAGCCCACACGT
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
+CTTTAGTAAGTATGTTCGCC
++
+ABBBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
+CCATAACACTTGGGGGTAGC
++
+3>>A@FFFFFFFGGGFEECF
+@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
+GTATCCGACATCTGGTTCCT
++
+A>ABAFA?D@AFGGGGFGGG
+@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
+TAGCTAAAGTGAACTGTATC
++
+@?AAAFFBFBFFFGGFGFGG
+@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
+GATCACAGGTCTATCACCCT
++
+ABBAAFFF@FFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
+GACAGATACTGCGACATAGG
++
+BBBBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
+GTATCCGACATCTGGTTCCT
++
+BBBAAFABDBBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
+GTATGTTCGCCTGTAATATT
++
+BABBBFFDABBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
+GTATGTTCGCCTGTAATATT
++
+BBBCCFFFDEDEGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
+ATATTGAACGTAGGTGCGAT
++
+ABCBBFFFFFAFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
+AATAAGACATCACGATGGAT
++
+BBBBBFBBFFFFGGGGCEGG
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
+CTTCAGGGTCATAAAACCTA
++
+3AABABFFBFFFGCFGGGGG
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
+AACTGTATCCGACATCTGGT
++
+ABBBA@FFFFBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
+GCTAAAGTGAACTGTATCCG
++
+1AAAAF1B13BBBEGGGFGG
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
+GTTATTATTATGTCCTACAA
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
+TAGGTGCGATAAATAATAGG
++
+BCCBAFFBCCCCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
+ATAATAGGATGAGGCAGGAA
++
+BCCCBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
+TTAGTAAGTATGTTCGCCTG
++
+AAAACFFFFFFFGGGFGGGG
+@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
+CTTTAGTAAGTATGTTCGCC
++
+3ABBBFFFFF@FGGGGGGGC
+@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
+ATTGAACGTAGGTGCGATAA
++
+BBBBBFFF?FFAGFBFGGEG
+@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
+TTATTATGTCCTACAAGCAT
++
+CCBBCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
+ATCAAAGACAGATACTGCGA
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
+AATCAAAGACAGATACTGCG
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
+AAATAGCCCACACGTTCCCC
++
+BBB@A4FFFBBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
+GGGTCATAAAACCTAAATAG
++
+?A3AA?DFFFFFGGGGGGGF
+@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
+TAATAGGATGAGGCAGGAAT
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
+AAAGTGAACTGTATCCGACA
++
+>AAA3BDFFFFDGBFFEEGG
+@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
+CCTGTAATATTGAACGTAGG
++
+AABBBFFFFFBBF5BGGFGA
+@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
+GTAATATTGAACGTAGGTGC
++
+33>A?FFFDFBFGCG4EGFG
+@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
+TAGTAAGTATGTTCGCCTGT
++
+BABAADFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
+AATTAACACACTTTAGTAAG
++
+BBBBBFFFFBBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
+TAACACTTGGGGGTAGCTAA
++
+AAA?1CFFFFADGEEGFGB1
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
+CTGTAATATTGAACGTAGGT
++
+BBBBBFFFFFFFGGGGGGCB
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
+CATTCAATTGTTATTATTAT
++
+ABBBAFFFFFFFFGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
+AAATAATAGGATGAGGCAGG
++
+BBBBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
+ATAAATAATAGGATGAGGCA
++
+AABBBFFFFFFFCGGGCGGG
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
+ACTTGGGGGTAGCTAAAGTG
++
+3ABB?FAB?DAAGGGFBGGB
+@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
+ATCAAAGACAGATACTGCGA
++
+ABAAAFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
+GCCAACACGTTCCCCTTAAA
++
+>>A3>AAAACFFFGFGFGGG
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
+GTCCTACAAGCATTAATTAA
++
+3>3AAFFFFFAFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
+ACACACTTTAGTAAGTATGT
++
+CBCCDCFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
+TCCTACTTCAGGGTCATAAA
++
+AA3AAFFFFFFFGGGFGFFG
+@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
+GGCCCATAACACTTGGGGGT
++
+1>>1AAFFFFFBGGFE1EEE
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
+GTATCCGACATCTGGTTCCT
++
+AAABABBBBBBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
+CTGTAATATTGAACGTAGGT
++
+AABABFFFFFFFF4GGGGEB
+@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
+CGCCTGTAATATTGAACGTA
++
+AABBBBFFFFFFGGGFGGGG
+@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
+GGAATCAAAGACAGATACTG
++
+AA?AAFFFFFFFGGGGGFBF
+@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
+GATCACAGGTCTATCACCCT
++
+AA>1>11F1D1DDFFFFGGG
+@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
+CCTACAAGCATTAATTAATT
++
+>AAAAFFFBFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
+TAGTAAGTATGTTCGCCTGT
++
+ABBBBFFFFFFFGGGFGGGG
+@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
+CGTTCCCCTTAAATAAGACA
++
+>A33>FFFFFF@FFFGGGGF
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
+CTTTAGTAAGTATGTTCGCC
++
+AABCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
+TAAATAAGACATCACGATGG
++
+BBBBAFFBDFBFBGGGGGFG
+@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
+CCTAAATAGCCCACACGTTC
++
+11>>ABFFFFFFGGCGC1BB
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
+TCCTACAAGCATTAATTAAT
++
+CCCCCFFFFCFFGGGGGGGG
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/paired_hardtrimmed5_pair2_.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_hardtrimmed5_pair2_.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
+ATCTGGTTCCTACTTCAGGG
++
+BCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
+GTTATTATTATGTCCTACAA
++
+AAAAAFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
+GTATCCGACATCTGGTTCCT
++
+CCCCCFDDDDDFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
+ATTAATTAACACACTTTAGT
++
+33AA?DFD5BDFGGGFEBDG
+@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
+AATTAACACACTTTAGTAAG
++
+CCCCDFFFFCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
+ATTATGTCCTACAAGCATTA
++
+ABCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
+CGCTCCGGGCCCATAACACT
++
+>A@AAAAD2ADDFFGGGGGF
+@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
+CCTTAAATAAGACATCACGA
++
+ABBBBFFFFFFFGGGGGGGC
+@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
+ATATTGAACGTAGGTGCGAT
++
+BCCBCFFFFFBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
+ATCAAAGACAGATACTGCGA
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
+CACTTGGGGGTAGCTAAAGT
++
+BBBCBFF@CCBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
+GCCCACACGTTCCCCTTAAA
++
+ABCCBCCBBFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
+ATATTGAACGTAGGTGCGAT
++
+BBBBBFFFFFAFGG5GGGGG
+@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
+GTATCCGACATCTGGTTCCT
++
+AAABCFCABBCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
+CTCCGGGCCCATAACACTTG
++
+CCDCCCCCCBCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
+GTATCCGACATCTGGTTCCT
++
+CBCCCFCCCCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
+GTAGCTAAAGTGAACTGTAT
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
+GTATCCGACATCTGGTTCCT
++
+AAABAFCCCCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
+GCCCATAACACTTGGGGGTA
++
+BBBBBFFFBDFFGFBCG2EG
+@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
+AAAGCGAACTGTATCCGACA
++
+ABB?AABBBBFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
+AAGACAGATACTGCGACATA
++
+BBBBBFFFFFFFGGEEGGFG
+@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
+GACATAGGGTGCTCCGGCTC
++
+ABBBBFFFFBAAGGGFEFGG
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
+AAATAAGACATCACGATGGA
++
+3A3AAFFFFBFFGGG2CF?4
+@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
+TAACACTTGGGGGTAGCTAA
++
+CCCBCFFFFFCCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
+GTATCCGACATCTGGTTCCT
++
+BABCBFBACCCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
+ACGTAGGTGCGATAAATAAT
++
+CDDCCFCFFFBCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
+CACTTGGGGGTAGCTAAAGT
++
+ABA?AFBAB2>AFGGGGFFG
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
+CACGATGGATCACAGGTCTA
++
+AAABABCAFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
+ATTCAATTGTTATTATTATG
++
+AAAABFFFFFFFGGGGGGGC
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
+AGCTAAAGTGAACTGTATCC
++
+AAA?AFFF3DFFFGGBGFF3
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
+CTACAAGCATTAATTAATTA
++
+BBBBBFFBFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
+GGCCCATAACACTTGGGGGT
++
+CCCCCCFFFFFFGGGGGGGE
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
+ATAAATAATAGGATGAGGCA
++
+CCCCBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
+ATTGAACGTAGGTGCGATAA
++
+CDDDDFFFBFFCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
+TTAGTAAGTATGTTCGCCTG
++
+AA1A1DFFFFFBGG3A111F
+@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
+GTCCTACAAGCATTAATTAA
++
+A3AAAFFFFFCFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
+GTTATTATTATGTCCTACAA
++
+AABCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
+TATGCTCGCCTGTAATATTG
++
+EDCCDFFCEEDCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
+TAATAGGATGAGGCAGGAAT
++
+CCCCCFBFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
+TCTGGTTCCTACTTCAGGGT
++
+BABA?DFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
+GGGGGTAGCTAAAGTGAACT
++
+ABB@3>AAFFFFFEAGGGFG
+@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
+TAGCTAAAGTGAACTGTATC
++
+CBCBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
+TTATGATTATGTCCTACAAG
++
+BAAAAFFFBFFFGGGGGGFF
+@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
+AACCTAAATAGCCCACACGT
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
+CTTTAGTAAGTATGTTCGCC
++
+ABBBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
+CCATAACACTTGGGGGTAGC
++
+3>>A@FFFFFFFGGGFEECF
+@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
+GTATCCGACATCTGGTTCCT
++
+A>ABAFA?D@AFGGGGFGGG
+@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
+TAGCTAAAGTGAACTGTATC
++
+@?AAAFFBFBFFFGGFGFGG
+@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
+GATCACAGGTCTATCACCCT
++
+ABBAAFFF@FFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
+GACAGATACTGCGACATAGG
++
+BBBBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
+GTATCCGACATCTGGTTCCT
++
+BBBAAFABDBBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
+GTATGTTCGCCTGTAATATT
++
+BABBBFFDABBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
+GTATGTTCGCCTGTAATATT
++
+BBBCCFFFDEDEGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
+ATATTGAACGTAGGTGCGAT
++
+ABCBBFFFFFAFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
+AATAAGACATCACGATGGAT
++
+BBBBBFBBFFFFGGGGCEGG
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
+CTTCAGGGTCATAAAACCTA
++
+3AABABFFBFFFGCFGGGGG
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
+AACTGTATCCGACATCTGGT
++
+ABBBA@FFFFBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
+GCTAAAGTGAACTGTATCCG
++
+1AAAAF1B13BBBEGGGFGG
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
+GTTATTATTATGTCCTACAA
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
+TAGGTGCGATAAATAATAGG
++
+BCCBAFFBCCCCGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
+ATAATAGGATGAGGCAGGAA
++
+BCCCBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
+TTAGTAAGTATGTTCGCCTG
++
+AAAACFFFFFFFGGGFGGGG
+@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
+CTTTAGTAAGTATGTTCGCC
++
+3ABBBFFFFF@FGGGGGGGC
+@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
+ATTGAACGTAGGTGCGATAA
++
+BBBBBFFF?FFAGFBFGGEG
+@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
+TTATTATGTCCTACAAGCAT
++
+CCBBCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
+ATCAAAGACAGATACTGCGA
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
+AATCAAAGACAGATACTGCG
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
+AAATAGCCCACACGTTCCCC
++
+BBB@A4FFFBBBGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
+GGGTCATAAAACCTAAATAG
++
+?A3AA?DFFFFFGGGGGGGF
+@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
+TAATAGGATGAGGCAGGAAT
++
+CCCCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
+AAAGTGAACTGTATCCGACA
++
+>AAA3BDFFFFDGBFFEEGG
+@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
+CCTGTAATATTGAACGTAGG
++
+AABBBFFFFFBBF5BGGFGA
+@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
+GTAATATTGAACGTAGGTGC
++
+33>A?FFFDFBFGCG4EGFG
+@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
+TAGTAAGTATGTTCGCCTGT
++
+BABAADFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
+AATTAACACACTTTAGTAAG
++
+BBBBBFFFFBBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
+TAACACTTGGGGGTAGCTAA
++
+AAA?1CFFFFADGEEGFGB1
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
+CTGTAATATTGAACGTAGGT
++
+BBBBBFFFFFFFGGGGGGCB
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
+CATTCAATTGTTATTATTAT
++
+ABBBAFFFFFFFFGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
+AAATAATAGGATGAGGCAGG
++
+BBBBBFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
+ATAAATAATAGGATGAGGCA
++
+AABBBFFFFFFFCGGGCGGG
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
+ACTTGGGGGTAGCTAAAGTG
++
+3ABB?FAB?DAAGGGFBGGB
+@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
+ATCAAAGACAGATACTGCGA
++
+ABAAAFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
+GCCAACACGTTCCCCTTAAA
++
+>>A3>AAAACFFFGFGFGGG
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
+GTCCTACAAGCATTAATTAA
++
+3>3AAFFFFFAFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
+ACACACTTTAGTAAGTATGT
++
+CBCCDCFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
+TCCTACTTCAGGGTCATAAA
++
+AA3AAFFFFFFFGGGFGFFG
+@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
+GGCCCATAACACTTGGGGGT
++
+1>>1AAFFFFFBGGFE1EEE
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
+GTATCCGACATCTGGTTCCT
++
+AAABABBBBBBFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
+CTGTAATATTGAACGTAGGT
++
+AABABFFFFFFFF4GGGGEB
+@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
+CGCCTGTAATATTGAACGTA
++
+AABBBBFFFFFFGGGFGGGG
+@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
+GGAATCAAAGACAGATACTG
++
+AA?AAFFFFFFFGGGGGFBF
+@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
+GATCACAGGTCTATCACCCT
++
+AA>1>11F1D1DDFFFFGGG
+@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
+CCTACAAGCATTAATTAATT
++
+>AAAAFFFBFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
+TAGTAAGTATGTTCGCCTGT
++
+ABBBBFFFFFFFGGGFGGGG
+@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
+CGTTCCCCTTAAATAAGACA
++
+>A33>FFFFFF@FFFGGGGF
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
+CTTTAGTAAGTATGTTCGCC
++
+AABCCFFFFFFFGGGGGGGG
+@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
+TAAATAAGACATCACGATGG
++
+BBBBAFFBDFBFBGGGGGFG
+@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
+CCTAAATAGCCCACACGTTC
++
+11>>ABFFFFFFGGCGC1BB
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
+TCCTACAAGCATTAATTAAT
++
+CCCCCFFFFCFFGGGGGGGG
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/sanger_full_range_report_results1.txt
--- a/test-data/sanger_full_range_report_results1.txt Tue Jul 30 06:26:49 2019 -0400
+++ b/test-data/sanger_full_range_report_results1.txt Fri Oct 08 09:57:52 2021 +0000
@@ -3,21 +3,24 @@
==========================
Input filename: input_1.fastq
Trimming mode: single-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Unable to auto-detect most prominent adapter from the first specified file (count Nextera: 0, count smallRNA: 0, count Illumina: 0)
+Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length before a sequence gets removed: 20 bp
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq
-Processing reads on 1 core in single-end mode ...
-Finished in 0.00 s (1608 us/read; 0.04 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.01 s (5282 µs/read; 0.01 M reads/minute).
=== Summary ===
@@ -31,10 +34,10 @@
=== Adapter 1 ===
-Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times.
+Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times
No. of allowed errors:
-0-9 bp: 0; 10-13 bp: 1
+1-9 bp: 0; 10-13 bp: 1
Bases preceding removed adapters:
A: 0.0%
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/sanger_full_range_report_results1gz.txt
--- a/test-data/sanger_full_range_report_results1gz.txt Tue Jul 30 06:26:49 2019 -0400
+++ b/test-data/sanger_full_range_report_results1gz.txt Fri Oct 08 09:57:52 2021 +0000
@@ -3,11 +3,14 @@
==========================
Input filename: input_1.fastq.gz
Trimming mode: single-end
-Trim Galore version: 0.6.3
-Cutadapt version: 2.4
-Number of cores used for trimming: 1
+Trim Galore version: 0.6.7
+Cutadapt version: 3.4
+Python version: could not detect
+Number of cores used for trimming: 4
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
+Unable to auto-detect most prominent adapter from the first specified file (count Illumina: 0, count Nextera: 0, count smallRNA: 0)
+Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
@@ -15,10 +18,10 @@
Output file will be GZIP compressed
-This is cutadapt 2.4 with Python 3.7.3
-Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz
-Processing reads on 1 core in single-end mode ...
-Finished in 0.02 s (7871 us/read; 0.01 M reads/minute).
+This is cutadapt 3.4 with Python 3.9.6
+Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz
+Processing reads on 4 cores in single-end mode ...
+Finished in 0.01 s (5217 µs/read; 0.01 M reads/minute).
=== Summary ===
@@ -32,10 +35,10 @@
=== Adapter 1 ===
-Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times.
+Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times
No. of allowed errors:
-0-9 bp: 0; 10-13 bp: 1
+1-9 bp: 0; 10-13 bp: 1
Bases preceding removed adapters:
A: 0.0%
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/sanger_full_range_results1.fastq.gz
Binary file test-data/sanger_full_range_results1.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/sanger_full_range_results2.fastq.gz
Binary file test-data/sanger_full_range_results2.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/sanger_full_range_results3.fastq.gz
Binary file test-data/sanger_full_range_results3.fastq.gz has changed
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/trimmed_polyA_reads_pair1.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/trimmed_polyA_reads_pair1.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
+ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
++
+BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
+GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
++
+AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A//
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF.
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
+ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG
++
+33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
+AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT
++
+3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
+@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1
+ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT
++
+CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
+CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT
++
+ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
+CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC
++
+AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.-
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
+ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC
++
+AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
+AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
++
+AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
+CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
++
+BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
+@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1
+GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
++
+CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
+ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
++
+CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
+ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC
++
+CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
+TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
++
+AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/:
+@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
+TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT
++
+CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0
+@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
+TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
++
+BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/
+@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT
++
+A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/
+@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1
+TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
++
+@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9--
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9
+@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1
+GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
++
+BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9
+@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1
+GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
++
+BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
+ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
++
+ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
+AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG
++
+BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A//
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
+CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT
++
+3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
+AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
++
+ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC-
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
+GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC
++
+1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
+GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT
++
+CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
+TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC
++
+BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/
+@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
+CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT
++
+AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
+TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG
++
+AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;///
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
+CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
++
+BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA;
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
+CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
++
+ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
+AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC
++
+BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
+ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG
++
+AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
+ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC
++
+3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B;
+@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1
+ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG
++
+ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
+GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
++
+>>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
+GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG
++
+3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
+ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG
++
+CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>-
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
+TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
++
+AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A-
+@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1
+GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
++
+1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT
++
+AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A.
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
+CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC
++
+AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//..
+@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1
+CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG
++
+AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF
+@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1
+TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
++
+ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:.../////
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
+CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT
++
+AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE.
+@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1
+TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC
++
+BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
+TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC
++
+CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
diff -r 084bbd8ba7b8 -r cd7e644cae1d test-data/trimmed_polyA_reads_pair2.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/trimmed_polyA_reads_pair2.fastqsanger Fri Oct 08 09:57:52 2021 +0000
@@ -0,0 +1,396 @@
+@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
+ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
++
+BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
+@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
+GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
++
+AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A//
+@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF.
+@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
+ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG
++
+33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9
+@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
+AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT
++
+3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
+@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1
+ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT
++
+CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D
+@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
+CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT
++
+ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9
+@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
+CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC
++
+AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.-
+@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
+ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC
++
+AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB
+@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
+AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
++
+AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
+@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
+CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
++
+BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
+@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1
+GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
++
+CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF
+@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
+ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
++
+CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0
+@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
+ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC
++
+CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0
+@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
+TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
++
+AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/:
+@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
+TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT
++
+CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0
+@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
+TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
++
+BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/
+@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT
++
+A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/
+@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1
+TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
++
+@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9--
+@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
++
+BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9
+@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1
+GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
++
+BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9
+@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1
+GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
++
+BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF
+@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
+ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
++
+ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
+@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
+AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG
++
+BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A//
+@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
+CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT
++
+3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/
+@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
+AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
++
+ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC-
+@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
+GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC
++
+1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9
+@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
+GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT
++
+CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/
+@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
+TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC
++
+BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/
+@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
+CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT
++
+AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9
+@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
+TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG
++
+AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;///
+@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
+CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
++
+BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA;
+@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
+CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
++
+ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9
+@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
+AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC
++
+BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD
+@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
+ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG
++
+AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9
+@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
+ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC
++
+3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B;
+@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1
+ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG
++
+ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF
+@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
+GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
++
+>>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
+@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
+GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG
++
+3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/
+@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
+ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG
++
+CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>-
+@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
+TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
++
+AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A-
+@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1
+GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
++
+1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9
+@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
+GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT
++
+AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A.
+@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
+CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC
++
+AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//..
+@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1
+CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG
++
+AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF
+@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1
+TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
++
+ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:.../////
+@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
+CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT
++
+AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE.
+@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1
+TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC
++
+BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
+@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
+TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC
++
+CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
diff -r 084bbd8ba7b8 -r cd7e644cae1d trim_galore.xml
--- a/trim_galore.xml Tue Jul 30 06:26:49 2019 -0400
+++ b/trim_galore.xml Fri Oct 08 09:57:52 2021 +0000
@@ -1,50 +1,10 @@
-
-
+
Quality and adapter trimmer of reads
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
- ^[ACTGNactgn]*$
-
-
-
-
-
-
-
-
- ^[ACTGNactgn]*$
-
-
-
-
- Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed.
- This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality.
- (--three_prime_clip_R1)
-
-
- Instructs Trim Galore! to remove N bp from the 3' end of read 2 after
- adapter/quality trimming has been performed. This may remove some unwanted bias from
- the 3' end that is not directly related to adapter sequence or basecall quality.
-
-
+ macros.xml
-
- trim-galore
-
+
+
trim_galore --version
@@ -92,6 +52,9 @@
trim_galore
+ ## according the develpers 4 cores could be a sweet spot, anything above has diminishing returns
+ --cores \${GALAXY_SLOTS:-4}
+
## we only support fastqsanger
--phred33
@@ -177,6 +140,18 @@
--dont_gzip
#end if
+ ## Trimming settings
+ #if $trimming.settingsType == 'custom'
+ #if $trimming.hardtrim5
+ --hardtrim5 $trimming.hardtrim5
+ #end if
+ #if $trimming.hardtrim3
+ --hardtrim3 $trimming.hardtrim3
+ #end if
+ $trimming.clock
+ $trimming.polyA
+ #end if
+
## Trim Galore is finished, rename the output if compressed
&&
if [ -f input_1_trimmed.fq.gz ] ; then mv input_1_trimmed.fq.gz input_1_trimmed.fq ; fi
@@ -188,12 +163,37 @@
if [ -f input_1_unpaired_1.fq.gz ] ; then mv input_1_unpaired_1.fq.gz input_1_unpaired_1.fq ; fi
&&
if [ -f input_2_unpaired_2.fq.gz ] ; then mv input_2_unpaired_2.fq.gz input_2_unpaired_2.fq ; fi
+ &&
+ if [ -f input_1.clock_UMI.R1.fq.gz ] ; then mv input_1.clock_UMI.R1.fq.gz input_1.clock_UMI.R1.fq ; fi
+ &&
+ if [ -f input_2.clock_UMI.R2.fq.gz ] ; then mv input_2.clock_UMI.R2.fq.gz input_2.clock_UMI.R2.fq ; fi
+
+ ## Rename hardtrimmed files
+ #if $trimming.settingsType == 'custom'
+ &&
+ if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq.gz input_1_hardtrim.fq ; fi
+ &&
+ if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq.gz input_2_hardtrim.fq ; fi
+ &&
+ if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq.gz input_1_hardtrim.fq ; fi
+ &&
+ if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq.gz input_2_hardtrim.fq ; fi
+ &&
+ if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq input_1_hardtrim.fq ; fi
+ &&
+ if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq input_2_hardtrim.fq ; fi
+ &&
+ if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq input_1_hardtrim.fq ; fi
+ &&
+ if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq input_2_hardtrim.fq ; fi
+ #end if
## Trim Galore! run is finished. Move the report files to the proper place
#if $params.settingsType == "custom" and $params.report:
&&
cat ./*_trimming_report.txt > '$report_file'
#end if
+ && ls -lah
]]>
@@ -226,7 +226,7 @@
-
+
@@ -261,7 +261,7 @@
-
+
@@ -274,35 +274,84 @@
label="Screen quality-trimmed sequences for 'CAA' or 'CGA' at the start of the read and, if found, removes the first two basepairs" />
+
+
+
+
+
+
+
+
+
+
+
+
+
+
singlePaired['sPaired'] == "single"
+ trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+
+ singlePaired['sPaired'] == "single"
+ trimming['settingsType'] == "custom"
+ trimming['hardtrim3'] != '' or trimming['hardtrim5'] != ''
+
+
+
singlePaired['sPaired'] == "paired_collection"
+ trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+ trimming['clock'] == False
-
+
+
params['settingsType'] == "custom"
params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"
singlePaired['sPaired'] == "paired_collection"
+ trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+
+
+
+
+ singlePaired['sPaired'] == "paired_collection"
+ trimming['settingsType'] == "custom"
+ trimming['hardtrim3'] or trimming['hardtrim5']
+
+
+
+
+
+
+ singlePaired['sPaired'] == "paired_collection"
+ trimming['settingsType'] == "custom"
+ trimming['clock']
+
+
+
singlePaired['sPaired'] == "paired"
+ trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+ trimming['clock'] == False
singlePaired['sPaired'] == "paired"
+ trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+ trimming['clock'] == False
params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"
singlePaired['sPaired'] == "paired"
+
+
+ singlePaired['sPaired'] == "paired"
+ trimming['settingsType'] == 'custom'
+ trimming['hardtrim3'] or trimming['hardtrim5']
+
+
+
+ singlePaired['sPaired'] == "paired"
+ trimming['settingsType'] == 'custom'
+ trimming['hardtrim3'] or trimming['hardtrim5']
+
+
+
+
+ singlePaired['sPaired'] == "paired"
+ trimming['settingsType'] == 'custom'
+ trimming['clock']
+
+
+
+ singlePaired['sPaired'] == "paired"
+ trimming['settingsType'] == 'custom'
+ trimming['clock']
+
+
params['settingsType'] == "custom"
params['report'] == True
@@ -325,50 +404,50 @@
-
+
-
+
-
+
-
+
-
+
-
+
-
+
-
+
-
+
@@ -378,7 +457,7 @@
-
+
@@ -389,7 +468,7 @@
-
+
@@ -414,7 +493,7 @@
-
+
@@ -439,6 +518,54 @@
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
-
+