Mercurial > repos > big-tiandm > mirplant2
diff count_rfam_express.pl @ 8:3ed8026af2d5 draft
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author | big-tiandm |
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date | Fri, 25 Jul 2014 05:20:08 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/count_rfam_express.pl Fri Jul 25 05:20:08 2014 -0400 @@ -0,0 +1,1800 @@ +#!/usr/bin/perl -w +#Filename: +#Author: Tian Dongmei +#Email: tiandm@big.ac.cn +#Date: 2013/7/19 +#Modified: +#Description: +my $version=1.00; + +use strict; +use Getopt::Long; +use File::Basename; + +my %opts; +GetOptions(\%opts,"i=s","o=s","tag:s","h"); +if (!(defined $opts{i} and defined $opts{o} ) || defined $opts{h}) { #necessary arguments +&usage; +} + +my $filein=$opts{'i'}; +my $fileout=$opts{'o'}; + +my $marks=defined $opts{'tag'} ? $opts{'tag'} : ""; + +if(!(defined $opts{'tag'})){ + my $line=`head -1 $filein`; + my @tmp=split/\t/,$line; + $tmp[0]=~/:([\d|_]+)_x(\d+)$/; + my @ss=split/_/,$1; + for (my $i=1;$i<=@ss;$i++) { + $marks .="Smp$i;"; + } +} + +my @marks=split/\;/,$marks; + +my %rfam_key; +while(<DATA>){ + chomp; + if(/^(\S+)\s+(\S+)$/){ + $rfam_key{$1}=$2; + } +} + + +my %reads; +my %tags; +open IN,"<$filein"; +while (my $aline=<IN>) { + chomp $aline; + my @tmp=split/\t/,$aline; + $tmp[0]=~/:([\d|_]+)_x(\d+)$/; + + my @exp=split/_/,$1; + my @tag=split/\;/,$tmp[2]; + + if (defined $rfam_key{$tag[0]}) { + for (my $i=0;$i<@exp;$i++) { + $reads{$rfam_key{$tag[0]}}[$i]+=$exp[$i]; + $tags{$rfam_key{$tag[0]}}[$i]++ if($exp[$i]!=0); + } + }else{ + for (my $i=0;$i<@exp;$i++) { + $reads{other}[$i]+=$exp[$i]; + $tags{other}[$i]++ if($exp[$i]!=0); + } + } + +} +close IN; + +$"="\t"; ##### @array print in \t +open OUT,">$fileout"; +print OUT "####################################\n# small RNA expressed reads number #\n####################################\n"; +print OUT "#RNAname\t@marks\n"; +foreach my $key (keys %reads) { + print OUT $key; + for (my $i=0;$i<@{$reads{$key}} ;$i++) { + print OUT "\t",$reads{$key}[$i]; + } + print OUT "\n"; +} + +print OUT "\n\n####################################\n# small RNA expressed tags number #\n####################################\n"; +print OUT "#RNAname\t@marks\n"; + +foreach my $key (keys %tags) { + print OUT $key; + for (my $i=0;$i<@{$reads{$key}} ;$i++) { + if(defined $tags{$key}[$i]){print OUT "\t",$tags{$key}[$i];} + else{print OUT "\t0";} + } + print OUT "\n"; +} + +close OUT; +$"=" "; ##### @array print in \t + +sub usage{ +print <<"USAGE"; +Version $version +Usage: +$0 -i -tag -o +options: +-i input file# rfam bowtie bwt. format mapping result +-tag [string] sample marks# eg. sampleA;sampleB;sampleC +-o output file + +-h help +USAGE +exit(1); +} + +__DATA__ +RF00635 lncRNA +RF01868 lncRNA +RF01869 lncRNA +RF01870 lncRNA +RF01871 lncRNA +RF01872 lncRNA +RF01873 lncRNA +RF01874 lncRNA +RF01875 lncRNA +RF01876 lncRNA +RF01877 lncRNA +RF01878 lncRNA +RF01879 lncRNA +RF01880 lncRNA +RF01881 lncRNA +RF01882 lncRNA +RF01883 lncRNA +RF01884 lncRNA +RF01885 lncRNA +RF01886 lncRNA +RF01887 lncRNA +RF01888 lncRNA +RF01889 lncRNA +RF01890 lncRNA +RF01891 lncRNA +RF01892 lncRNA +RF01893 lncRNA +RF01894 lncRNA +RF01904 lncRNA +RF01905 lncRNA +RF01906 lncRNA +RF01907 lncRNA +RF01908 lncRNA +RF01909 lncRNA +RF01928 lncRNA +RF01929 lncRNA +RF01930 lncRNA +RF01931 lncRNA +RF01932 lncRNA +RF01933 lncRNA +RF01934 lncRNA +RF01935 lncRNA +RF01946 lncRNA +RF01947 lncRNA +RF01948 lncRNA +RF01950 lncRNA +RF01951 lncRNA +RF01952 lncRNA +RF01953 lncRNA +RF01954 lncRNA +RF01955 lncRNA +RF01956 lncRNA +RF01957 lncRNA +RF01958 lncRNA +RF01961 lncRNA +RF01962 lncRNA +RF01963 lncRNA +RF01964 lncRNA +RF01965 lncRNA +RF01966 lncRNA +RF01967 lncRNA +RF01968 lncRNA +RF01969 lncRNA +RF01970 lncRNA +RF01971 lncRNA +RF01972 lncRNA +RF01973 lncRNA +RF01974 lncRNA +RF01975 lncRNA +RF01976 lncRNA +RF01977 lncRNA +RF01978 lncRNA +RF01979 lncRNA +RF01980 lncRNA +RF01981 lncRNA +RF01983 lncRNA +RF01984 lncRNA +RF01985 lncRNA +RF01986 lncRNA +RF01987 lncRNA +RF01992 lncRNA +RF02038 lncRNA +RF02039 lncRNA +RF02040 lncRNA +RF02041 lncRNA +RF02042 lncRNA +RF02043 lncRNA +RF02044 lncRNA +RF02045 lncRNA +RF02046 lncRNA +RF02047 lncRNA +RF02085 lncRNA +RF02086 lncRNA +RF02087 lncRNA +RF02089 lncRNA 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