Mercurial > repos > big-tiandm > mirplant2
diff preProcess.xml @ 50:7b5a48b972e9 draft
Uploaded
author | big-tiandm |
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date | Fri, 05 Dec 2014 00:11:02 -0500 |
parents | c75593f79aa9 |
children | 8b8c356e6db5 |
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--- a/preProcess.xml Wed Dec 03 02:03:27 2014 -0500 +++ b/preProcess.xml Fri Dec 05 00:11:02 2014 -0500 @@ -12,7 +12,7 @@ <!--command interpreter="perl">miPlant.pl -i $input -format $format -gfa $gfa -idx $index -pre $pre -mat $mat -rfam $rfam -idx2 $idx2 -D $D -a $a -M $M -min $min -max $max -mis $mis -e $e -f $f -v $v -r $r -dis $dis -flank $flank -mfe $mfe -t $t -o $output</command--> - <command interpreter="perl">miRPlant.pl + <command interpreter="perl">preProcess.pl ## Change this to accommodate the number of threads you have available. -t \${GALAXY_SLOTS:-4} -path \$SCRIPT_PATH @@ -25,7 +25,16 @@ ## Do or not annotate rfam non-miRNA RNAs #if $params.annotate_rfam == "yes": - -rfam $rfam + ## prepare Rfam bowtie index + #set rfam_index_path = '' + #if str($params.annotate_rfam.reference_rfam.source) == "history": + bowtie-build "$params.annotate_rfam.reference_rfam.own_file" rfam; ln -s "$params.annotate_rfam.reference_rfam.own_file" rfam.fa; + #set rfam_index_path = 'rfam' + #else: + #set rfam_index_path = $params.annotate_rfam.reference_rfam.index.fields.path + #end if + + -rfam ${rfam_index_path}.fa -idx2 $rfam_index_path -v $v #end if ## prepare bowtie index @@ -37,7 +46,7 @@ #set index_path = $reference_genome.index.fields.path #end if - -format $format -gfa ${index_path}.fa -idx $index_path -a $a -M $mapnt -min $min -max $max -mis $mismatch -v $v > run.log + -format $format -gfa ${index_path}.fa -idx $index_path -phred $phred -a $a -M $mapnt -min $min -max $max -mis $mismatch > run.log </command> <inputs> @@ -75,6 +84,10 @@ <!--param name="gfa" type="data" label="genome sequence fasta file"/--> <!--param type="data" name="index" label="genome sequence bowtie index"/--> + <param name="phred" type="select" lable="input quals are Phred+64 or Phred+33" multiple="false"> + <option value="64">Phred+64</option> + <option value="33" selected="true">Phred+33</option> + </param> <param name="a" type="text" value="ATCTCGTATG" label="3' adapter sequence" /> <param name="mapnt" type="integer" value="8" label="minimum adapter map nts" /> <param name="min" type="integer" value="19" label="minimum microRNA length" /> @@ -87,8 +100,27 @@ <option value="no">no</option> </param> <when value="yes"> - <param name="rfam" type="data" label="rfam sequence file" /> - <!--param type="data" name="idx2" label="rfam sequence bowtie index " --> + <!--param name="rfam" type="data" label="rfam sequence file" /--> + <when value="yes"> + <!--param name="rfam" type="data" label="rfam sequence file" /--> + <conditional name="reference_rfam"> + <param name="source" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options"> + <option value="indexed">Use a built-in index</option> + <option value="history">Use one from the history</option> + </param> + <when value="indexed"> + <param name="index" type="select" label="Select a reference" help="If your reference of interest is not listed, contact the Galaxy team"> + <options from_data_table="rfam_bowtie_indexes"> + <filter type="sort_by" column="2"/> + <validator type="no_options" message="No indexes are available for the selected input dataset"/> + </options> + </param> + </when> + <when value="history"> + <param name="own_file" type="data" format="fasta" metadata_name="dbkey" label="Select the reference" /> + </when> + </conditional> + <param name="v" type="integer" value="0" label="report end-to-end hits less than v mismatches for rfam mapping"/> </when> </conditional> <!-- params --> @@ -112,6 +144,8 @@ <data format="txt" name="Rfam not mapped FASTA file" from_work_dir="preProcess/rfam_match/rfam_not_mapped.fa" label="${tool.name} on ${on_string}: Rfam not mapped FASTA file"> <filter>(params['annotate_rfam'] == 'Yes')</filter> </data> + <data format="html" name="input config" from_work_dir="preProcess/input_config" label="${tool.name} on ${on_string}: input config"/> + </outputs> <help>